This document provides an overview of a GMO investigator kit and workshop for teaching students how to detect genetically modified crops. The kit allows students to extract and amplify DNA from food samples, perform PCR and electrophoresis to identify GMOs. It contains materials for 8 student workstations to complete the activity over 3 lab sessions. The workshop introduces students to genetically modified foods and guides them through extracting DNA from food products, setting up PCR reactions, electrophoresis of PCR products, and analyzing results to identify GMOs. Common genetically modified food crops approved in the US are also listed.

1. TOPIC – DETECTION OF
GENETICALLY MODIFIED CROP
AND TRANSGENE IN NONGM
CROPS
SEED TECHNOLOGY (AGRI-2015)

2. •Submitted To –
Dr.Arvind kumar
•Submitted by
•Priyanka Yadav -19SBAS2080109
•Arjun Singh-19SBAS2080060
•Akshay choudhary- 19
SBAS2080050
•Siddarth Sirohi- 19
SBAS2080037

3. Stan Hitomi
Coordinator – Math & Science
San Ramon Valley Unified School District
Danville, CA
Kirk Brown
Lead Instructor, Edward Teller Education Center
Science Chair, Tracy High School
and Delta College, Tracy, CA
Sherri Andrews, Ph.D.
Curriculum and Training Specialist
Bio-Rad Laboratories
Essy Levy, M.Sc.
Curriculum and Training Specialist
Bio-Rad Laboratories
GMO
Investigator Kit
Instructors

4. Why teach
GMO testing? • Inquiry-based
• Real-world test
• Environmental Science
• Plant Physiology
• Genetics and biotechnology
• Bioinformatics/Data Mining
• Standards-based

6. GMO
Investigator
Kit
Advantages
• Extract and amplify DNA from different food
samples
• Perform genuine diagnostic procedures
• Use PCR and electrophoresis to find GMO
foods
• Sufficient materials for 8 student
workstations
• Complete the activity in three 45 minute
lab sessions
• Laboratory extensions: Real-Time PCR

7. GMO
Workshop
Time Line • Introduction to GM foods
• DNA extraction of food products
• Set up PCR reactions
• Electrophorese PCR products
• Analysis and interpretation of
results

8. GMO
Investigator
Procedures
Overview

9. What is a
GMO?
"genetically modified organism (GMO)"
an organism in which the genetic material
has been altered in a way that does not
occur naturally by mating and/or natural
recombination

10. US Approval for GM food crops
•Corn
•Soy
•Papaya
•Canola
•Potato
•Chicory
•Rice
•Squash
•Sugarbeet
•Tomatoes
Approval does not necessarily mean these
crops are distributed
Database of GM crops: www.agbios.com
Which foods
contain GM
product?

11. Which foods
contain GM
product?
0
10
20
30
40
50
60
70
80
90
100
1995
1996
1997
1998
1999
2000
2001
2002
2003
2004
%
of
all
crop
planted
GM corn GM soy
Sources: 1996-1999 Fernandez and McBride, 2000-2004:
USDA, National Agriculture Statistics Service, Acreage.