SlideShare a Scribd company logo
Hari Narayan Kushwaha, M. Pharm. (Gold Medalist), PhD
Research Scientist, (From 30th
March 2015)
TEVA API India Pvt Ltd., Plot No.: 2-G,2-H,2-I, Ecotech-II, Udyog Vihar, Greater Noida-201306.
Mobile No.: 09582232006 E-Mail: drharinarayan.cdri@gmail.com
Passport No.: K2483305 Date of Issue: 26/03/2012 Date of Issue: 25/03/2022
CAREERS:
Challenging, resourceful and pioneering skills with demonstrated achievements in LC-MS/MS,
Impurity profiling, Genotoxic evaluation and quantitation, HPLC/UPLC method development and
validation for impurity profiling and assay, pharmacokinetics and metabolism, molecular, cellular
studies etc. for solving problems of Drug Discovery. Proficient in communication, association,
deliberate planning and team constructions.
DBT-POSTDOCTORAL RESEARCH ASSOCIATE (2013-2015)
CSIR-Indian Institute of Toxicology Research, M. G. Marg, Lucknow, India - 226001
Project: Role Of Phytochemicals For The Protection Of Human Skin Against Harmful Effect Of Solar
Ultraviolet Radiations funded by Department of Biotechnology (DBT) Govt. of India.
EDUCATION:
Ph. D. (Pharmacy: April 8th
, 2013)
Duration: November 2008-November 2012.
Thesis Title: “Drug-Drug Interaction and Pharmacokinetic studies of Novel Trioxane Antimalarial
Molecules with Antiepileptic Drugs”.
Mentor : Dr. S. K. Singh, Senior Principal Scientist & In-Charge, Pharmacokinetics &
Metabolism Division, CSIR-Central Drug Research Institute, Lucknow, U.P., India.
(www.cdriindia.org)
University: Faculty of Pharmacy, Integral University, Lucknow, U.P., India.
(www.integraluniversity.ac.in)
M. Pharm.
Duration: May 2005-April 2007
Subject: Pharmaceutical Biotechnology, Gold Medal (9.06, CGPA),
College: Kanak Manjari Institute of Pharmaceutical Sciences, Rourkela, Orissa.
(www.kmips.com)
University: Biju Patnaik University of Technology, Rourkela, Orissa, India. (www.bput.ac.in)
B. Pharm.
Duration: September 2000-November 2004
Subject: Pharmacy (72.15 %)
College: College of Pharmaceutical Sciences, Berhampur, Orissa. (www.cpsmohuda.org)
University: Biju Patnaik University of Technology, Rourkela, Orissa, India.
TECHNICAL EXPERTISE AND RESEARCH EXPERIENCE:
 Genotoxic Impurity identification, elucidation, profiling and validation including carryover
studies.
 API method development, impurity profiling, validation, report preparation.
 Different dosage form preparation and their evaluation.
 Preparation of standard operating protocols (SOP), Standard Testing protocols (STP) and
data analysis and compiling of final report by collating different results.
 Well versed with GLP, ICH and US-FDA guidelines for preclinical and clinical studies including
analytical method development as well as GMP and cGMP guidelines.
 Analytical and Bioanalytical :
 Working knowledge, method upgradation and expertise in LC-MS/MS (4600-QTOF, API-4000,
4000Q-Trap, 5500Q-Trap ABSciex Biosystems), EMPOWER for HPLC-UV, UHPLC
(NEXERA, Waters, Agilent), Chorona Ion Chromatography, TLC, High Throughput Screening
1
Hari Narayan Kushwaha, M. Pharm. (Gold Medalist), PhD
(HTS) System (Freedom EVO of TECAN), 2-D Gel electrophoresis (Bio-Rad, USA),
spectrophotometer and fluorescence plate reader.
 Method development, validation and stability studies as per ICH and/or US-FDA guidelines in
biological matrices: blood, serum, plasma, urine, feces, bile and different tissues.
 Quantitative and qualitative analysis of in-vitro and in-vivo metabolites, photodegraded drugs
after ultraviolet radiation exposure as well as impurity profiling.
 Pharmacokinetics and Metabolic Studies:
 Expertise in dosing, sample collection, bile duct cannulation and in-situ absorption study to
rats, rabbits and monkeys.
 Pharmacokinetic, toxicokinetic, Protein binding, blood partitioning and drug permeability
studies of drugs and new chemical entities in rat, rabbit, monkey and human models to
establish absorption, distribution, metabolism and excretion.
 In-vitro metabolic studies in liver S-9/microsomal fractions and metabolite profiling.
 Cellular :
 Plasmid isolation and cloning, isotopic labeling for protein expression and purification by Ni-
NTA and FPLC.
 Well versed with cell culture techniques of primary cells and cell lines for assessment of drug
phototoxicity through MTT and NRU assay.
 Analysis of Reactive Oxygen Species (ROS) generation, DNA damage using fluorescence
microscopy and comet assay technique and cell cycle regulation through Flow cytometry.
 Characterization of photosensitive marker genes and protein expression through Real Time
PCR, western blot and immunofluorescence technique.
SOFTWARES: Empower, Chromsword, Analyst, LightSight, Metabolite ID, Phoneix-WinNonLin,
NLME, IVIVC, ChemBioDraw, PD Quest 2-D spot analysis.
PROJECTS APPROVED, AWARDS AND OTHER ACTIVITIES:
 DBT-Postdoctoral Research Associate project on “Role Of Phytochemicals For The
Protection Of Human Skin Against Harmful Effect Of Solar Ultraviolet Radiations”
awarded in 2013 by Indian Institute of Science (IISc), Bangalore for Department of
Biotechnology, Govt. of India, New Delhi, India.
 CSIR- Direct Senior Research Fellowship project on “Pharmacokinetics And Metabolic
Studies Of CDRI Novel Compounds” was awarded by Council of Scientific & Industrial
Research, New Delhi, India from November 2008- November 2012.
 Active reviewer in journal: Drug Research.
 Life member of
i Laboratory Animal Science Association of India [Membership No: 448].
ii Indian Society of Mass spectrometry [Membership No: LM789].
iii Indian Photobiology System.
 Successfully passed General course on Intellectual Property Rights of WIPO worldwide
academy, Switzerland.
 Team CDRI was awarded with “CSIR Technology Award-Innovation for 2009” on
discovery and development of Trioxane Antimalarial agents 97/78 and 99/411.
 Best Poster Award 2009: “LC-MS/MS assay for quantification of 97-78 and its metabolite
97-63, a promising trioxane antimalarial in human plasma” at 11
th
ISMAS Triennial
International Conference on Mass Spectrometry, November 24-28, Hyderabad, India.
 Best Poster Award 2014 for “Caspase 3 dependent apoptotic cell death induced by
Benzophenone-1 through mitochondrial signaling pathway at environmental UVR” at
2
Hari Narayan Kushwaha, M. Pharm. (Gold Medalist), PhD
National Conference on Ethanopharmacology and biotechnology in drug development:
Prospects and challenges, November 14-15, Jhansi, India.
 2
nd
position in Debate competition on World Aids Day (2003) organized by Rotract Club of
College of Pharmaceutical Sciences, Mohuda, Berhampur, Orissa, India.
 Lecturer in Department of Pharmacy, Rameshwaram Institute of Technology and
Management, Lucknow U.P., India from 02/04/2007 to 03/11/2008.
 Assisted Six Ph.D scholars and provided training to eight post-graduate (M. Pharma/ M.S.-
Pharma/ M. Tech.).
PATENTS AWARDED / FILED:
1. P. C. Verma, J. Gupta, D. P. Singh, V. Gupta, H. N. Kushwaha, , et. al. Antidiabetic action of
pregnane-oximino-ether compounds. (Application No.: DSTM/PAT/03/2012, application date
10-05-2012)
2. P. C. Verma, J. Gupta, D. P. Singh, V. Gupta, H. N. Kushwaha, , et. al. Pregnane-Oximino-
Aminoalkylethers And Process For Preparation Thereof, Useful As Antidiabetic And
Antidyslipidemic Agents. (US Application No.: 14/763,480 filed in 2016)
PUBLICATIONS:
1. G.C. Kisku, Markandeya, H Kushwaha, S Arora. Environmental Health Risk Estimation of
Heavy Metals Accumulated in Soil and Cultivated Plants Irrigated with Industrial Effluents. Adv
Recycling Waste Manag. 1 (2016) 108.
2. D. Chopra, L. Ray, A. Dwivedi, S. K. Tiwari, J. Singh, K. P. Singh, H. N. Kushwaha, S. Jahan,
A. Pandey, S. K. Gupta, R. K. Chaturvedi, A. B. Pant,R. S. Ray, K. C. Gupta. Photoprotective
efficiency of PLGA-curcumin nanoparticles versus curcumin through the involvement of
ERK/AKT pathway under ambient UV-R exposure in HaCaT cell line. Biomaterials 84 (2016)
25-41
3. S. Goyal, S. K. Amar, A. Dwivedi, S. F. Mujtaba, H. N. Kushwaha et. al. Photosensitized 2-
amino-3-hydroxypyridine-induced mitochondrial apoptosis via Smac/DIABLO in human skin
cells. Toxicology and Applied Pharmacology 297 (2016) 12–21
4. A. K. Srivastav, S. F. Mujtaba, A. Dwivedi, S. K. Amar, S. Goyal, A. Verma, Hari N. Kushwaha
et. al. Photosensitized rose Bengal-induced phototoxicity on humanmelanoma cell line under
natural sunlight exposure. Journal of Photochemistry & Photobiology, B: Biology 156 (2016)
87–99
5. S. Goyal, S. K. Amar, D. Dubey, M. K. Pal, J. Singh, A. Verma, H. N. Kushwaha, R. S. Ray.
Involvement of cathepsin B in mitochondrial apoptosis byp-phenylenediamine under ambient
UV radiation. Journal of Hazardous Materials 300 (2015) 415–425
6. S. K. Amar, S. Goyal, S. F. Mujtaba, A. Dwivedi, H. N. Kushwaha et. al. Role of type I and
type II reactions in DNA damage and activation of caspase 3 via mitochondrial pathway
induced by photosensitized benzophenone. Toxicology Letters 235 (2015) 84–95
7. S.F. Mujtaba, A. Dwivedi , N. Yadav, R Shekar, H. N. Kushwaha, et. al. Superoxide mediated
photomodification and DNA damage induced apoptosis by benz(a)anthracene via
mitochondrial mediated pathway. (Accepted November, 2014) Journal of Photochemistry and
Photobiology B: Biology
3
Hari Narayan Kushwaha, M. Pharm. (Gold Medalist), PhD
8. Y. Singh, M. Hidau, A. Misra, H. N. Kushwaha, et. al. UFLC Method Development and
Validation of a Novel Triethylamine containing Thiophene S006-830-an Antitubercular
molecule and its Application to Pharmacokinetic and Bioavailability Studies in SD Rats.
Accepted (October, 2014) Drug Testing and Analysis.
9. Y. Singh*, H. N. Kushwaha*, A. Misra, et. al. Time Course of the Changes in Novel Trioxane
Antimalarial, 99/411 Pharmacokinetics upon Antiepileptic Drugs co-administration in SD Rats.
Malaria Treatment and Research. Accepted (September, 2014)(* Equal Authorship)
10. H. N. Kushwaha, N. K. Mohan, et. al. Pharmacokinetic Study And Bioavailability Of A Novel
Synthetic Trioxane Antimalarial Compound 97/63 In Rats. Malaria Treatment and Research.
Volume 2014 (2014), Article ID 759392.
11. R. P. Singh, S. Sabarinath, H. N. Kushwaha, et. al. Multiple-dose pharmacokinetics of a
novel, synthetic trioxane-antimalarial compound 97/78 in rats using LC-MS/MS method.
International Journal of Bioassays, 3(07): 3122-3126 (2014).
12. A. Ahmad, A. K. Chauhan, H. N. Kushwaha, et. al. Preferential desulfurization of Dibenzyl
sulfide by an isolated Gordonia sp. IITR100. 3Biotech (BTEC), (May, 2014) DOI
10.1007/s13205-014-0221-1.
13. A. K. Tripathi, A. Dwivedi, M. K. Pal, N. Rastogi, S. Ali, B. H. M. Prabhu, H. N. Kushwaha, et.
al. Attenuated neuroprotective effect of riboflavin under UV-B irradiation via miR-203/c-Jun
signaling pathway in vivo and in vitro. Journal of Biomedical Science, 21:39 (May, 2014).
14. N. Shafiq, S. Rajagopalan, H. N. Kushwaha, et. al. Single ascending dose safety and
pharmacokinetics of CDRI-97/78: First in human study of a novel antimalarial drug. Malaria
Research and Treatment, (January, 2014) http://dx.doi.org/10.1155/2014/372521.
15. A. Dwivedi , S. F. Mujtaba, N. Yadav, H. N. Kushwaha, et. al. Cellular and molecular
mechanism of ofloxacin induced apoptotic cell death under ambient UV-A and sunlight
exposure. Free Radical Research, 48(3): 333-46 (2014).
16. N. Gautam, R. P. Singh, H. N. Kushwaha, et. al. Liquid chromatography tandem mass
spectrometry method for determination of antidiabetic chalcones derivative S001-469 in rat
plasma, urine and feces: application to pharmacokinetic study. Drug Research, 64, 377-383
(2014).
17. A. Misra, H. N. Kushwaha, N. Gautam, et. al. Bioanalytical LC-MS/MS method development
and validation of novel antidiabetic candidate S007-1261 in rat plasma and its application to
pharmacokinetic and oral bioavailability studies. Drug Research, (October, 2013) DOI:
10.1055/s-0033-1358739.
18. R. S. Ray, S. F. Mujtaba, A. Dwivedi, N. Yadav, A. Verma, H. N. Kushwaha, et. al. Singlet
oxygen mediated DNA damage induced phototoxicity by Ketoprofen resulting in mitochondrial
depolarization and lysosomal destabilization, Toxicology, 15;314(2-3):229-37 (2013).
19. H. N. Kushwaha, A. Misra, et. al. Effect of Carbamazepine on pharmacokinetic parameters of
CDRI-97/78, after Coadministration in rats, ArzneimittelForschung / Drug Research, 63(6):
282-8 (2013).
20. N. Yadav, A. Dwivedi, S.F. Mujtaba, H.N. Kushwaha, et. al. Ambient UVA-induced expression
of p53 and apoptosis in human skin melanoma A375 cell line by quinine, Photochemistry and
Photobiology, 89(3): 655-64 (2013).
21. H. N. Kushwaha, N. Gautam, et. al. Intersex effect of lamotrigine on pharmacokinetic
parameters of CDRI-97/78, a novel trioxane antimalarial compund in rats, Arzneimittel-
Forschung / Drug Research, 62 (6): 274-279 (2012).
22. A. Dwivedi, S. F. Mujtaba, H. N. Kushwaha, et. al. Photosensitizing Mechanism and
4
Hari Narayan Kushwaha, M. Pharm. (Gold Medalist), PhD
Identification of Levofloxacin Photoproducts at Ambient UV Radiation, Photochemistry and
Photobiology, 88: 344–355 (2012).
23. H. N. Kushwaha, N. Gautam, et. al. Liquid chromatographic tandem mass spectrometric
assay for simultaneous quantification of 97/78 and its in-vivo metabolite 97/63, a novel
trioxane antimalarial in human plasma and its application to protein binding study.
Arzneimittel-Forschung / Drug Research, 61 (7) 425-432 (2011)
24. N. Gautam, H. N. Kushwaha, et. al. LC-MS/MS assay for quantification of a novel antidiabetic
flavone derivative S002-857 in rat plasma, urine and feces: Application in pharmacokinetic
studies. Chromatographia, 73 (9) 905-912 (2011)
25. N. Gautam, H.N. Kushwaha, et. al. In-situ absorption, protein binding and pharmacokinetic
studies of new synthetic antidiabetic and antidyslipidemic flavone derivative S002-853 in rats.
Journal of pharmacy and pharmacology. 62, 604-609 (2010).
PUBLISHED ABSTRACTS FROM CONFERENCES:
1. H. N. Kushwaha, N. Gautam, et. al. Effect of carbamazepine- an antiepileptic on
pharmacokinetics profile of an antimalarial trioxane in rats. Medicinal Chemistry Research, 19,
S68-69, 2010.
2. N. Gautam, H. N. Kushwaha, et. al. Excretion study of a novel antidiabetic S002-853 in rat
urine and feces quantified by LC-MS/MS method. Medicinal Chemistry Research, 19, S91-92,
2010.
3. S. K. Amar,S. Gyyal, D. Dubey, H. N. Kushwaha, et. al. Caspase 3 dependent apoptotic cell
death induced by Benzophenone-1 through mitochondrial signaling pathway at environmental
UVR. Journal of Biomedical and Therapeutics Sciences (ISSN: 2394-2274), 1(1): P-77, 2014
4. S. Goyal, S. K. Amar, A. Dwivedi, D. Chopra, S. F. Mujtaba, H. N. Kushwaha, et. al. Role of
Smac/DIABLO in mitochondrial dependent apoptosis by 2-amino-3-hydroxypyridine under
ambient UV R exposure. Journal of Biomedical and Therapeutics Sciences (ISSN: 2394-
2274), 1(1): P-93, 2014
MANUSCRIPT COMMUNICATED:
1. R. P. Singh, S. Sabarinath, H. N. Kushwaha, et. al. Comparative pharmacokinetics and oral
bioavailability of Racemate and individual stereo-isomers of CDRI-97/78, a new antimalarial
agent in monkeys. Drug Research.
2. S. K. Amar, S. Goyal, S.F. Mujtaba, A. Dwivedi, H. N. Kushwaha, D. Chopra, A. verma, P. K.
Singh, R. S. Ray. Oxidative stress and DNA damage mediated apoptosis and identification of
marker proteins by photosensitized Benzophenone under environmental UV radiation.
Archieves of Toxicology.
MANUSCRIPT UNDER PREPARATION:
1. H. N. Kushwaha, A. Dwivedi , A. K. Tripathi, et. al. LC-MS/MS cellular uptake analysis
revealed photoproduct formation of photoexcited CDRI novel trioxane antimalarial causing
type I mediated apoptotic cell death of HaCaT cell line.
5
Hari Narayan Kushwaha, M. Pharm. (Gold Medalist), PhD
2. N. Gautam, H. N. Kushwaha, S. Pandey, S. K. Singh. Mass spectrometric development and
validation of 99/411 in different tissues and matrices as well as single and multiple dose
pharmacokinetics.
3. R. P. Singh, S. Sabarinath, H. N. Kushwaha, et. al. Multiple-dose pharmacokinetics confirm
no accumulation and dose proportionality of 97-78, a novel antimalarial agent in Rhesus
monkeys.
REVIEWS:
1. S. Goyal, S.K. Amar, H.N. Kushwaha, et. al. Toxicological potential of Parabens- a widely
used preservative. GJMS. (Accepted November, 2014)
BOOK CHAPTER:
5. H.N. Kushwaha, N. Gautam, S. K. Singh. LC-MS/MS assay for quantification of 97/78 and its
metabolite 97/63, a promising trioxane antimalarial in human plasma, 11th
ISMAS-TRICON-
2009 (ISBN: 978-81-904442-2-4) S.K. Aggrarwal, P.G. Jaison et al. (Editors), p 342-346
(2009).
PRESENTATIONS:
INVITED LECTURE:
1. Ist Faculty Development Programme (FDP) sponsored by Dr. A.P.J. Abdul Kalam Technical
University, Lucknow, from 27.01.2016 to 31.01.2016 at Shri Ram Murti Smarak College of
Engg. & Tech. (Pharmacy) Bareilly, U.P.
ORAL:
1. A. Dwivedi, S. F. Mujtaba, H. N. Kushwaha, et. al. Photosensitizing action of ofloxacin at
ambient UV-A exposure: a mechanistic study. National Seminar on Reactive Oxygen Species:
Roles in Animal and Plant Biology, Lucknow University on 23-24 December, 2011.
2. H. N. Kushwaha, A. Khurmi, S. K. Singh. Development and evaluation of nifedipine as
transdermal gel. Symposium on Dendrimer in Nano Drug Delivery, CSIR-National Botanical
Research Institute, Lucknow on 24-25 December, 2011.
POSTERS:
1. H. N. Kushwaha, N. Gautam, S. K. Singh. LC-MS/MS assay for quantification of 97/78 and its
metabolite 97/63, a promising trioxane antimalarial in human plasma, 11th
ISMAS-TRICON-
2009 November 24-28, 2009, Hyderabad, India. (Best poster)
2. H. N. Kushwaha, N. Gautam, H. Kumar, S.K. Singh. Effect of gabapentin - an antiepileptic on
pharmacokinetics profile of 97-78, an antimalarial trioxane in rats, 2nd
International
Symposium on Drug Metabolism and Pharmacokinetics, Applications toward Drug Discovery
and Development, February 2010, NIPER, Punjab, India.
3. H. N. Kushwaha, N. Gautam, S. K. Singh. Effect of carbamazepine- an antiepileptic on
pharmacokinetics profile of an antimalarial trioxane in rats. 4th International Symposium on
Current Trends in Drug Discovery Research, February 17-21, 2010 at CSIR-CDRI, Lucknow,
6
Hari Narayan Kushwaha, M. Pharm. (Gold Medalist), PhD
India.
4. N. Gautam, H. N. Kushwaha, S.K. Singh. Excretion study of a novel antidiabetic S002-853 in
rat urine and feces quantified by LC-MS/MS method, 4th International Symposium on Current
Trends in Drug Discovery Research, February 17-21, 2010 at CSIR-CDRI, Lucknow, India.
5. N. Gautam, H. N. Kushwaha, H. Kumar, R. Pratap, S. K. Singh. Pharmacokinetic and
excretion studies of a novel antidiabetic S002-857 in rats. 2nd
International Symposium on
Drug Metabolism and Pharmacokinetics, Applications toward Drug Discovery and
Development, February 2010, NIPER, Punjab, India.
6. N. Gautam, H. Kumar, H. N. Kushwaha, S. K. Singh. Pharmacokinetics, SGF/SIF, and in-situ
absorption study of a novel antidiabetic compound S002-857 synthesized by CDRI, 2nd
International Symposium on Drug Metabolism and Pharmacokinetics, Applications toward
Drug Discovery and Development, February 2010, NIPER, Punjab, India.
7. H. N. Kushwaha, N. Gautam, A. Misra, B. Singh, S. K. Singh. Effect of Phenytoin- an
antiepileptic on pharmacokinetics profile of an antimalarial trioxane in rats. 3rd
International
Symposium on Drug Metabolism and Pharmacokinetics, Applications toward Drug Discovery
and Development, February 2011, NIPER, Punjab, India.
8. H. N. Kushwaha, H. H. Siddiqui, S. K. Singh. Comparative pharmacokinetic drug
interaction between Gabapentin, an antiepileptic and CDRI-97/78, an antimalarial in male and
female rats. AICTE Seminar held at I.T.S. College of Pharmacy, Ghaziabad on 5-6 August,
2011.
9. H. N. Kushwaha, H. Kumar, A. Misra, Y. Singh, S. K. Singh. Pharmacokinetic interaction
between CDRI-97/78, novel trioxane antimalarial and Topiramate in male and female SD rats.
4th
NIPER (RBL)-CDRI Symposium on Medicinal Chemisrty and Pharmaceutical Sciences at
CSIR-Central Drug Research Institute, Lucknow on 23-25 Feburary, 2012.
10. A. Verma, H. N. Kushwaha, M.C. Pant, R.S. Ray. Protection against ultraviolet radiation
induced damage in human keratanocytes by oleanolic and ursolic acid. X oint Annual
Conference of ISMOCD & IAE, National Institute of Malaria Research Goa Medical College
and Hospital, Goa on 12-12 October, 2014
11. S. K. Amar,S. Gyyal, D. Dubey, H. N. Kushwaha, et. al. Caspase 3 dependent apoptotic cell
death induced by Benzophenone-1 through mitochondrial signaling pathway at environmental
UVR. National Conference on Ethanopharmacology and biotechnology in drug development:
Prospects and challenges, Budelkhand University, Jhansi on 14-15 November, 2014. (Best
Poster)
12. S. Goyal, S. K. Amar, A. Dwivedi, D. Chopra, S. F. Mujtaba, H. N. Kushwaha, et. al. Role of
Smac/DIABLO in mitochondrial dependent apoptosis by 2-amino-3-hydroxypyridine under
ambient UV R exposure. National Conference on Ethanopharmacology and biotechnology in
drug development: Prospects and challenges, Budelkhand University, Jhansi on 14-15
November, 2014.
PERSONAL INFORMATION:
Name: Hari Narayan Kushwaha
Nationality: Indian (by Birth)
Date of birth: 01st May 1980
Place of birth: Durjanpur, Dist. Ballia, Uttar Pradesh, India
Gender: Male
7
Hari Narayan Kushwaha, M. Pharm. (Gold Medalist), PhD
Permanent Address: House at 33A, Near Maurya Caterers, Kanchan Nagar,
Mohamadpur Khatri, Kalyanpur, Luknow (U.P.)-226022, India.
REFERENCES:
1. Dr. Ratan Singh Ray
Principal Scientist and Head,
Photobiology Division,
CSIR-Indian Institute of Toxicology Research,
Lucknow -226 001 (U.P.) India.
Mobile No.: 09935882991
E-mail: ratanray.2013@rediffmail.com
2. Dr. S. K. SINGH
Pharmacokinetics And Metabolism Division,
CSIR-Central Drug Research Institute,
Lucknow -226 001 (U.P.) India,
Mobile No.: 0919450360338,
E-mail: shiokumar@yahoo.com.
: DECLARATION:
I hereby declare that the above information is factually correct to the best of my
knowledge. I also understand that I will be disqualified if any of the information is found to be wrong.
Date:
Place: Greater Noida ------------------------------------
Signature of the Candidate
8

More Related Content

What's hot

Manish adhikari Resume
Manish adhikari ResumeManish adhikari Resume
Manish adhikari Resume
Manish Adhikari
 
CV Girish sept
CV Girish septCV Girish sept
CV Girish septGirish Rai
 
2016 CV Upendra Nayek
2016 CV Upendra Nayek2016 CV Upendra Nayek
2016 CV Upendra Nayekupendra nayek
 
CURRICULUM VITAE
CURRICULUM  VITAECURRICULUM  VITAE
CURRICULUM VITAEAnuj Thakur
 
Cv dr rahul hajare-september-2017
Cv dr rahul hajare-september-2017Cv dr rahul hajare-september-2017
Cv dr rahul hajare-september-2017
Rrahul Hajare (Post Doc., Ph.D)
 
Bio data- kundan
Bio data- kundanBio data- kundan
Bio data- kundan
Dr Kundan Prasad
 
Cv industry research updated
Cv industry research updatedCv industry research updated
Cv industry research updated
Pawan Yadav
 
Curriculum vitae
Curriculum vitaeCurriculum vitae
Curriculum vitae
Dr.Kamal Uddin zaidi
 
curriculum vitae
curriculum vitaecurriculum vitae
curriculum vitae
Dr.Kamal Uddin zaidi
 
Curriculum Vitae - Sagar Naskar
Curriculum Vitae - Sagar NaskarCurriculum Vitae - Sagar Naskar
Curriculum Vitae - Sagar Naskarsagar naskar
 
Sudharsan cv new
Sudharsan cv newSudharsan cv new
Sudharsan cv newhashiny
 

What's hot (19)

Manish adhikari Resume
Manish adhikari ResumeManish adhikari Resume
Manish adhikari Resume
 
Rajesh Resume2016
Rajesh Resume2016Rajesh Resume2016
Rajesh Resume2016
 
CV_Murlidhar Jadhav_BT_2015
CV_Murlidhar Jadhav_BT_2015CV_Murlidhar Jadhav_BT_2015
CV_Murlidhar Jadhav_BT_2015
 
CV- Dr. MANOJ
CV- Dr. MANOJCV- Dr. MANOJ
CV- Dr. MANOJ
 
CV Girish sept
CV Girish septCV Girish sept
CV Girish sept
 
2016 CV Upendra Nayek
2016 CV Upendra Nayek2016 CV Upendra Nayek
2016 CV Upendra Nayek
 
Mariah H Goodall CV
Mariah H Goodall CVMariah H Goodall CV
Mariah H Goodall CV
 
dtpcv Dec 2016
dtpcv Dec 2016dtpcv Dec 2016
dtpcv Dec 2016
 
CURRICULUM VITAE
CURRICULUM  VITAECURRICULUM  VITAE
CURRICULUM VITAE
 
Cv dr rahul hajare-september-2017
Cv dr rahul hajare-september-2017Cv dr rahul hajare-september-2017
Cv dr rahul hajare-september-2017
 
Biplab Giri_CV
Biplab Giri_CVBiplab Giri_CV
Biplab Giri_CV
 
dtpcv M2015
dtpcv M2015dtpcv M2015
dtpcv M2015
 
Prashantha_Docking
Prashantha_DockingPrashantha_Docking
Prashantha_Docking
 
Bio data- kundan
Bio data- kundanBio data- kundan
Bio data- kundan
 
Cv industry research updated
Cv industry research updatedCv industry research updated
Cv industry research updated
 
Curriculum vitae
Curriculum vitaeCurriculum vitae
Curriculum vitae
 
curriculum vitae
curriculum vitaecurriculum vitae
curriculum vitae
 
Curriculum Vitae - Sagar Naskar
Curriculum Vitae - Sagar NaskarCurriculum Vitae - Sagar Naskar
Curriculum Vitae - Sagar Naskar
 
Sudharsan cv new
Sudharsan cv newSudharsan cv new
Sudharsan cv new
 

Viewers also liked

Jayanthi putri k [130641310]
Jayanthi putri k [130641310]Jayanthi putri k [130641310]
Jayanthi putri k [130641310]
jayanthiputri
 
Melissa Newell Resume (1) (2)
Melissa Newell Resume (1) (2)Melissa Newell Resume (1) (2)
Melissa Newell Resume (1) (2)Melissa Newell
 
Kelompok 13
Kelompok 13Kelompok 13
Kelompok 13
jayanthiputri
 
Jayanthi putri k [130641310]
Jayanthi putri k [130641310]Jayanthi putri k [130641310]
Jayanthi putri k [130641310]
jayanthiputri
 
Jayanthi putri k [130641310]
Jayanthi putri k [130641310]Jayanthi putri k [130641310]
Jayanthi putri k [130641310]jayanthiputri
 
Presentation1
Presentation1Presentation1
Presentation1
Shoaib Sultan
 
NOISE REDUCTION IN GEAR TRAIN
NOISE REDUCTION IN GEAR TRAINNOISE REDUCTION IN GEAR TRAIN
NOISE REDUCTION IN GEAR TRAIN
Raju Gollangi
 
satyam scam (sonali kumari 2608)
satyam scam (sonali kumari 2608)satyam scam (sonali kumari 2608)
satyam scam (sonali kumari 2608)Sonali Chauhan
 
Financial function of excel
Financial function of excel Financial function of excel
Financial function of excel
Sonali Chauhan
 
Applied line card 2015
Applied line card  2015Applied line card  2015
Applied line card 2015
Christopher Emerson
 
NOISE REDUCTION IN GEAR TRAIN
NOISE REDUCTION  IN  GEAR TRAIN NOISE REDUCTION  IN  GEAR TRAIN
NOISE REDUCTION IN GEAR TRAIN
Raju Gollangi
 
Abbud_Capstone_Urban-Design_Toolkit
Abbud_Capstone_Urban-Design_ToolkitAbbud_Capstone_Urban-Design_Toolkit
Abbud_Capstone_Urban-Design_Toolkitfernandoabbud
 
Cv viktoria saxby_2017_svenska
Cv viktoria saxby_2017_svenskaCv viktoria saxby_2017_svenska
Cv viktoria saxby_2017_svenska
Viktoria Saxby
 

Viewers also liked (18)

Jayanthi putri k [130641310]
Jayanthi putri k [130641310]Jayanthi putri k [130641310]
Jayanthi putri k [130641310]
 
Melissa Newell Resume (1) (2)
Melissa Newell Resume (1) (2)Melissa Newell Resume (1) (2)
Melissa Newell Resume (1) (2)
 
Kelompok 13
Kelompok 13Kelompok 13
Kelompok 13
 
Jayanthi putri k [130641310]
Jayanthi putri k [130641310]Jayanthi putri k [130641310]
Jayanthi putri k [130641310]
 
Jayanthi putri k [130641310]
Jayanthi putri k [130641310]Jayanthi putri k [130641310]
Jayanthi putri k [130641310]
 
Presentation1
Presentation1Presentation1
Presentation1
 
NOISE REDUCTION IN GEAR TRAIN
NOISE REDUCTION IN GEAR TRAINNOISE REDUCTION IN GEAR TRAIN
NOISE REDUCTION IN GEAR TRAIN
 
satyam scam (sonali kumari 2608)
satyam scam (sonali kumari 2608)satyam scam (sonali kumari 2608)
satyam scam (sonali kumari 2608)
 
Financial function of excel
Financial function of excel Financial function of excel
Financial function of excel
 
Applied line card 2015
Applied line card  2015Applied line card  2015
Applied line card 2015
 
NOISE REDUCTION IN GEAR TRAIN
NOISE REDUCTION  IN  GEAR TRAIN NOISE REDUCTION  IN  GEAR TRAIN
NOISE REDUCTION IN GEAR TRAIN
 
Abbud_Capstone_Urban-Design_Toolkit
Abbud_Capstone_Urban-Design_ToolkitAbbud_Capstone_Urban-Design_Toolkit
Abbud_Capstone_Urban-Design_Toolkit
 
Resume_020717
Resume_020717Resume_020717
Resume_020717
 
Resume
ResumeResume
Resume
 
Cv viktoria saxby_2017_svenska
Cv viktoria saxby_2017_svenskaCv viktoria saxby_2017_svenska
Cv viktoria saxby_2017_svenska
 
Sethi_Anjali
Sethi_AnjaliSethi_Anjali
Sethi_Anjali
 
Resume
ResumeResume
Resume
 
Payal Gupta CV
Payal Gupta CVPayal Gupta CV
Payal Gupta CV
 

Similar to CV_Hari Narayan Kushwaha

Arvind Dabhade_CV (3)4
Arvind Dabhade_CV (3)4Arvind Dabhade_CV (3)4
Arvind Dabhade_CV (3)4Arvind Dabhade
 
Anusha S Daragshetti Resume
Anusha S Daragshetti ResumeAnusha S Daragshetti Resume
Anusha S Daragshetti ResumeAnusha Desai
 
Cv dr-rahul- hajare jan- 2017
Cv  dr-rahul- hajare   jan- 2017Cv  dr-rahul- hajare   jan- 2017
Cv dr-rahul- hajare jan- 2017
Rrahul Hajare (Post Doc., Ph.D)
 
Cv dr-rahul- hajare jan- 2017
Cv  dr-rahul- hajare   jan- 2017Cv  dr-rahul- hajare   jan- 2017
Cv dr-rahul- hajare jan- 2017
Rrahul Hajare (Post Doc., Ph.D)
 
Curriculum vitae bharat patnaik.doc
Curriculum vitae bharat patnaik.docCurriculum vitae bharat patnaik.doc
Curriculum vitae bharat patnaik.doc
Bharat Bhusan Patnaik
 
Anamika cv
Anamika cvAnamika cv
Anamika cv
Dr. Anamika Gupta
 
Curriculum vitae Bahaderjeet - cup
Curriculum vitae Bahaderjeet - cupCurriculum vitae Bahaderjeet - cup
Curriculum vitae Bahaderjeet - cupbahaderjit singh
 
Dr.B N Chaitanya
Dr.B N ChaitanyaDr.B N Chaitanya
Dr.B N Chaitanya
Dr. B N CHAITANYA
 
Dr. B. N. Chaitanya
Dr. B. N. Chaitanya Dr. B. N. Chaitanya
Dr. B. N. Chaitanya
Dr. B N CHAITANYA
 
Prof. Archana-moon-resume
Prof. Archana-moon-resumeProf. Archana-moon-resume
Prof. Archana-moon-resume
Prof. Archana Moon
 
Dr.GYM-CV
Dr.GYM-CVDr.GYM-CV
Dr.GYM-CVgymeti
 
Resume - Palur Gunasekar
Resume - Palur GunasekarResume - Palur Gunasekar
Resume - Palur GunasekarPalur Gunasekar
 
Cv dr rahul hajare-september-2017
Cv dr rahul hajare-september-2017Cv dr rahul hajare-september-2017
Cv dr rahul hajare-september-2017
Rrahul Hajare (Post Doc., Ph.D)
 

Similar to CV_Hari Narayan Kushwaha (20)

Arvind Dabhade_CV (3)4
Arvind Dabhade_CV (3)4Arvind Dabhade_CV (3)4
Arvind Dabhade_CV (3)4
 
Anusha S Daragshetti Resume
Anusha S Daragshetti ResumeAnusha S Daragshetti Resume
Anusha S Daragshetti Resume
 
Cv dr-rahul- hajare jan- 2017
Cv  dr-rahul- hajare   jan- 2017Cv  dr-rahul- hajare   jan- 2017
Cv dr-rahul- hajare jan- 2017
 
Cv dr-rahul- hajare jan- 2017
Cv  dr-rahul- hajare   jan- 2017Cv  dr-rahul- hajare   jan- 2017
Cv dr-rahul- hajare jan- 2017
 
Resume
ResumeResume
Resume
 
Curriculum vitae bharat patnaik.doc
Curriculum vitae bharat patnaik.docCurriculum vitae bharat patnaik.doc
Curriculum vitae bharat patnaik.doc
 
CV YBT full yamini 9-11-16
CV YBT full yamini  9-11-16CV YBT full yamini  9-11-16
CV YBT full yamini 9-11-16
 
RESUME-CKK
RESUME-CKKRESUME-CKK
RESUME-CKK
 
Anamika cv
Anamika cvAnamika cv
Anamika cv
 
Dr rahul-hajare cv-
Dr rahul-hajare cv-Dr rahul-hajare cv-
Dr rahul-hajare cv-
 
Curriculum vitae Bahaderjeet - cup
Curriculum vitae Bahaderjeet - cupCurriculum vitae Bahaderjeet - cup
Curriculum vitae Bahaderjeet - cup
 
Dr.B N Chaitanya
Dr.B N ChaitanyaDr.B N Chaitanya
Dr.B N Chaitanya
 
Dr. B. N. Chaitanya
Dr. B. N. Chaitanya Dr. B. N. Chaitanya
Dr. B. N. Chaitanya
 
CV_Parvez
CV_ParvezCV_Parvez
CV_Parvez
 
Prof. Archana-moon-resume
Prof. Archana-moon-resumeProf. Archana-moon-resume
Prof. Archana-moon-resume
 
Glory francis.
Glory francis.Glory francis.
Glory francis.
 
Dr.GYM-CV
Dr.GYM-CVDr.GYM-CV
Dr.GYM-CV
 
CV.1
CV.1CV.1
CV.1
 
Resume - Palur Gunasekar
Resume - Palur GunasekarResume - Palur Gunasekar
Resume - Palur Gunasekar
 
Cv dr rahul hajare-september-2017
Cv dr rahul hajare-september-2017Cv dr rahul hajare-september-2017
Cv dr rahul hajare-september-2017
 

CV_Hari Narayan Kushwaha

  • 1. Hari Narayan Kushwaha, M. Pharm. (Gold Medalist), PhD Research Scientist, (From 30th March 2015) TEVA API India Pvt Ltd., Plot No.: 2-G,2-H,2-I, Ecotech-II, Udyog Vihar, Greater Noida-201306. Mobile No.: 09582232006 E-Mail: drharinarayan.cdri@gmail.com Passport No.: K2483305 Date of Issue: 26/03/2012 Date of Issue: 25/03/2022 CAREERS: Challenging, resourceful and pioneering skills with demonstrated achievements in LC-MS/MS, Impurity profiling, Genotoxic evaluation and quantitation, HPLC/UPLC method development and validation for impurity profiling and assay, pharmacokinetics and metabolism, molecular, cellular studies etc. for solving problems of Drug Discovery. Proficient in communication, association, deliberate planning and team constructions. DBT-POSTDOCTORAL RESEARCH ASSOCIATE (2013-2015) CSIR-Indian Institute of Toxicology Research, M. G. Marg, Lucknow, India - 226001 Project: Role Of Phytochemicals For The Protection Of Human Skin Against Harmful Effect Of Solar Ultraviolet Radiations funded by Department of Biotechnology (DBT) Govt. of India. EDUCATION: Ph. D. (Pharmacy: April 8th , 2013) Duration: November 2008-November 2012. Thesis Title: “Drug-Drug Interaction and Pharmacokinetic studies of Novel Trioxane Antimalarial Molecules with Antiepileptic Drugs”. Mentor : Dr. S. K. Singh, Senior Principal Scientist & In-Charge, Pharmacokinetics & Metabolism Division, CSIR-Central Drug Research Institute, Lucknow, U.P., India. (www.cdriindia.org) University: Faculty of Pharmacy, Integral University, Lucknow, U.P., India. (www.integraluniversity.ac.in) M. Pharm. Duration: May 2005-April 2007 Subject: Pharmaceutical Biotechnology, Gold Medal (9.06, CGPA), College: Kanak Manjari Institute of Pharmaceutical Sciences, Rourkela, Orissa. (www.kmips.com) University: Biju Patnaik University of Technology, Rourkela, Orissa, India. (www.bput.ac.in) B. Pharm. Duration: September 2000-November 2004 Subject: Pharmacy (72.15 %) College: College of Pharmaceutical Sciences, Berhampur, Orissa. (www.cpsmohuda.org) University: Biju Patnaik University of Technology, Rourkela, Orissa, India. TECHNICAL EXPERTISE AND RESEARCH EXPERIENCE:  Genotoxic Impurity identification, elucidation, profiling and validation including carryover studies.  API method development, impurity profiling, validation, report preparation.  Different dosage form preparation and their evaluation.  Preparation of standard operating protocols (SOP), Standard Testing protocols (STP) and data analysis and compiling of final report by collating different results.  Well versed with GLP, ICH and US-FDA guidelines for preclinical and clinical studies including analytical method development as well as GMP and cGMP guidelines.  Analytical and Bioanalytical :  Working knowledge, method upgradation and expertise in LC-MS/MS (4600-QTOF, API-4000, 4000Q-Trap, 5500Q-Trap ABSciex Biosystems), EMPOWER for HPLC-UV, UHPLC (NEXERA, Waters, Agilent), Chorona Ion Chromatography, TLC, High Throughput Screening 1
  • 2. Hari Narayan Kushwaha, M. Pharm. (Gold Medalist), PhD (HTS) System (Freedom EVO of TECAN), 2-D Gel electrophoresis (Bio-Rad, USA), spectrophotometer and fluorescence plate reader.  Method development, validation and stability studies as per ICH and/or US-FDA guidelines in biological matrices: blood, serum, plasma, urine, feces, bile and different tissues.  Quantitative and qualitative analysis of in-vitro and in-vivo metabolites, photodegraded drugs after ultraviolet radiation exposure as well as impurity profiling.  Pharmacokinetics and Metabolic Studies:  Expertise in dosing, sample collection, bile duct cannulation and in-situ absorption study to rats, rabbits and monkeys.  Pharmacokinetic, toxicokinetic, Protein binding, blood partitioning and drug permeability studies of drugs and new chemical entities in rat, rabbit, monkey and human models to establish absorption, distribution, metabolism and excretion.  In-vitro metabolic studies in liver S-9/microsomal fractions and metabolite profiling.  Cellular :  Plasmid isolation and cloning, isotopic labeling for protein expression and purification by Ni- NTA and FPLC.  Well versed with cell culture techniques of primary cells and cell lines for assessment of drug phototoxicity through MTT and NRU assay.  Analysis of Reactive Oxygen Species (ROS) generation, DNA damage using fluorescence microscopy and comet assay technique and cell cycle regulation through Flow cytometry.  Characterization of photosensitive marker genes and protein expression through Real Time PCR, western blot and immunofluorescence technique. SOFTWARES: Empower, Chromsword, Analyst, LightSight, Metabolite ID, Phoneix-WinNonLin, NLME, IVIVC, ChemBioDraw, PD Quest 2-D spot analysis. PROJECTS APPROVED, AWARDS AND OTHER ACTIVITIES:  DBT-Postdoctoral Research Associate project on “Role Of Phytochemicals For The Protection Of Human Skin Against Harmful Effect Of Solar Ultraviolet Radiations” awarded in 2013 by Indian Institute of Science (IISc), Bangalore for Department of Biotechnology, Govt. of India, New Delhi, India.  CSIR- Direct Senior Research Fellowship project on “Pharmacokinetics And Metabolic Studies Of CDRI Novel Compounds” was awarded by Council of Scientific & Industrial Research, New Delhi, India from November 2008- November 2012.  Active reviewer in journal: Drug Research.  Life member of i Laboratory Animal Science Association of India [Membership No: 448]. ii Indian Society of Mass spectrometry [Membership No: LM789]. iii Indian Photobiology System.  Successfully passed General course on Intellectual Property Rights of WIPO worldwide academy, Switzerland.  Team CDRI was awarded with “CSIR Technology Award-Innovation for 2009” on discovery and development of Trioxane Antimalarial agents 97/78 and 99/411.  Best Poster Award 2009: “LC-MS/MS assay for quantification of 97-78 and its metabolite 97-63, a promising trioxane antimalarial in human plasma” at 11 th ISMAS Triennial International Conference on Mass Spectrometry, November 24-28, Hyderabad, India.  Best Poster Award 2014 for “Caspase 3 dependent apoptotic cell death induced by Benzophenone-1 through mitochondrial signaling pathway at environmental UVR” at 2
  • 3. Hari Narayan Kushwaha, M. Pharm. (Gold Medalist), PhD National Conference on Ethanopharmacology and biotechnology in drug development: Prospects and challenges, November 14-15, Jhansi, India.  2 nd position in Debate competition on World Aids Day (2003) organized by Rotract Club of College of Pharmaceutical Sciences, Mohuda, Berhampur, Orissa, India.  Lecturer in Department of Pharmacy, Rameshwaram Institute of Technology and Management, Lucknow U.P., India from 02/04/2007 to 03/11/2008.  Assisted Six Ph.D scholars and provided training to eight post-graduate (M. Pharma/ M.S.- Pharma/ M. Tech.). PATENTS AWARDED / FILED: 1. P. C. Verma, J. Gupta, D. P. Singh, V. Gupta, H. N. Kushwaha, , et. al. Antidiabetic action of pregnane-oximino-ether compounds. (Application No.: DSTM/PAT/03/2012, application date 10-05-2012) 2. P. C. Verma, J. Gupta, D. P. Singh, V. Gupta, H. N. Kushwaha, , et. al. Pregnane-Oximino- Aminoalkylethers And Process For Preparation Thereof, Useful As Antidiabetic And Antidyslipidemic Agents. (US Application No.: 14/763,480 filed in 2016) PUBLICATIONS: 1. G.C. Kisku, Markandeya, H Kushwaha, S Arora. Environmental Health Risk Estimation of Heavy Metals Accumulated in Soil and Cultivated Plants Irrigated with Industrial Effluents. Adv Recycling Waste Manag. 1 (2016) 108. 2. D. Chopra, L. Ray, A. Dwivedi, S. K. Tiwari, J. Singh, K. P. Singh, H. N. Kushwaha, S. Jahan, A. Pandey, S. K. Gupta, R. K. Chaturvedi, A. B. Pant,R. S. Ray, K. C. Gupta. Photoprotective efficiency of PLGA-curcumin nanoparticles versus curcumin through the involvement of ERK/AKT pathway under ambient UV-R exposure in HaCaT cell line. Biomaterials 84 (2016) 25-41 3. S. Goyal, S. K. Amar, A. Dwivedi, S. F. Mujtaba, H. N. Kushwaha et. al. Photosensitized 2- amino-3-hydroxypyridine-induced mitochondrial apoptosis via Smac/DIABLO in human skin cells. Toxicology and Applied Pharmacology 297 (2016) 12–21 4. A. K. Srivastav, S. F. Mujtaba, A. Dwivedi, S. K. Amar, S. Goyal, A. Verma, Hari N. Kushwaha et. al. Photosensitized rose Bengal-induced phototoxicity on humanmelanoma cell line under natural sunlight exposure. Journal of Photochemistry & Photobiology, B: Biology 156 (2016) 87–99 5. S. Goyal, S. K. Amar, D. Dubey, M. K. Pal, J. Singh, A. Verma, H. N. Kushwaha, R. S. Ray. Involvement of cathepsin B in mitochondrial apoptosis byp-phenylenediamine under ambient UV radiation. Journal of Hazardous Materials 300 (2015) 415–425 6. S. K. Amar, S. Goyal, S. F. Mujtaba, A. Dwivedi, H. N. Kushwaha et. al. Role of type I and type II reactions in DNA damage and activation of caspase 3 via mitochondrial pathway induced by photosensitized benzophenone. Toxicology Letters 235 (2015) 84–95 7. S.F. Mujtaba, A. Dwivedi , N. Yadav, R Shekar, H. N. Kushwaha, et. al. Superoxide mediated photomodification and DNA damage induced apoptosis by benz(a)anthracene via mitochondrial mediated pathway. (Accepted November, 2014) Journal of Photochemistry and Photobiology B: Biology 3
  • 4. Hari Narayan Kushwaha, M. Pharm. (Gold Medalist), PhD 8. Y. Singh, M. Hidau, A. Misra, H. N. Kushwaha, et. al. UFLC Method Development and Validation of a Novel Triethylamine containing Thiophene S006-830-an Antitubercular molecule and its Application to Pharmacokinetic and Bioavailability Studies in SD Rats. Accepted (October, 2014) Drug Testing and Analysis. 9. Y. Singh*, H. N. Kushwaha*, A. Misra, et. al. Time Course of the Changes in Novel Trioxane Antimalarial, 99/411 Pharmacokinetics upon Antiepileptic Drugs co-administration in SD Rats. Malaria Treatment and Research. Accepted (September, 2014)(* Equal Authorship) 10. H. N. Kushwaha, N. K. Mohan, et. al. Pharmacokinetic Study And Bioavailability Of A Novel Synthetic Trioxane Antimalarial Compound 97/63 In Rats. Malaria Treatment and Research. Volume 2014 (2014), Article ID 759392. 11. R. P. Singh, S. Sabarinath, H. N. Kushwaha, et. al. Multiple-dose pharmacokinetics of a novel, synthetic trioxane-antimalarial compound 97/78 in rats using LC-MS/MS method. International Journal of Bioassays, 3(07): 3122-3126 (2014). 12. A. Ahmad, A. K. Chauhan, H. N. Kushwaha, et. al. Preferential desulfurization of Dibenzyl sulfide by an isolated Gordonia sp. IITR100. 3Biotech (BTEC), (May, 2014) DOI 10.1007/s13205-014-0221-1. 13. A. K. Tripathi, A. Dwivedi, M. K. Pal, N. Rastogi, S. Ali, B. H. M. Prabhu, H. N. Kushwaha, et. al. Attenuated neuroprotective effect of riboflavin under UV-B irradiation via miR-203/c-Jun signaling pathway in vivo and in vitro. Journal of Biomedical Science, 21:39 (May, 2014). 14. N. Shafiq, S. Rajagopalan, H. N. Kushwaha, et. al. Single ascending dose safety and pharmacokinetics of CDRI-97/78: First in human study of a novel antimalarial drug. Malaria Research and Treatment, (January, 2014) http://dx.doi.org/10.1155/2014/372521. 15. A. Dwivedi , S. F. Mujtaba, N. Yadav, H. N. Kushwaha, et. al. Cellular and molecular mechanism of ofloxacin induced apoptotic cell death under ambient UV-A and sunlight exposure. Free Radical Research, 48(3): 333-46 (2014). 16. N. Gautam, R. P. Singh, H. N. Kushwaha, et. al. Liquid chromatography tandem mass spectrometry method for determination of antidiabetic chalcones derivative S001-469 in rat plasma, urine and feces: application to pharmacokinetic study. Drug Research, 64, 377-383 (2014). 17. A. Misra, H. N. Kushwaha, N. Gautam, et. al. Bioanalytical LC-MS/MS method development and validation of novel antidiabetic candidate S007-1261 in rat plasma and its application to pharmacokinetic and oral bioavailability studies. Drug Research, (October, 2013) DOI: 10.1055/s-0033-1358739. 18. R. S. Ray, S. F. Mujtaba, A. Dwivedi, N. Yadav, A. Verma, H. N. Kushwaha, et. al. Singlet oxygen mediated DNA damage induced phototoxicity by Ketoprofen resulting in mitochondrial depolarization and lysosomal destabilization, Toxicology, 15;314(2-3):229-37 (2013). 19. H. N. Kushwaha, A. Misra, et. al. Effect of Carbamazepine on pharmacokinetic parameters of CDRI-97/78, after Coadministration in rats, ArzneimittelForschung / Drug Research, 63(6): 282-8 (2013). 20. N. Yadav, A. Dwivedi, S.F. Mujtaba, H.N. Kushwaha, et. al. Ambient UVA-induced expression of p53 and apoptosis in human skin melanoma A375 cell line by quinine, Photochemistry and Photobiology, 89(3): 655-64 (2013). 21. H. N. Kushwaha, N. Gautam, et. al. Intersex effect of lamotrigine on pharmacokinetic parameters of CDRI-97/78, a novel trioxane antimalarial compund in rats, Arzneimittel- Forschung / Drug Research, 62 (6): 274-279 (2012). 22. A. Dwivedi, S. F. Mujtaba, H. N. Kushwaha, et. al. Photosensitizing Mechanism and 4
  • 5. Hari Narayan Kushwaha, M. Pharm. (Gold Medalist), PhD Identification of Levofloxacin Photoproducts at Ambient UV Radiation, Photochemistry and Photobiology, 88: 344–355 (2012). 23. H. N. Kushwaha, N. Gautam, et. al. Liquid chromatographic tandem mass spectrometric assay for simultaneous quantification of 97/78 and its in-vivo metabolite 97/63, a novel trioxane antimalarial in human plasma and its application to protein binding study. Arzneimittel-Forschung / Drug Research, 61 (7) 425-432 (2011) 24. N. Gautam, H. N. Kushwaha, et. al. LC-MS/MS assay for quantification of a novel antidiabetic flavone derivative S002-857 in rat plasma, urine and feces: Application in pharmacokinetic studies. Chromatographia, 73 (9) 905-912 (2011) 25. N. Gautam, H.N. Kushwaha, et. al. In-situ absorption, protein binding and pharmacokinetic studies of new synthetic antidiabetic and antidyslipidemic flavone derivative S002-853 in rats. Journal of pharmacy and pharmacology. 62, 604-609 (2010). PUBLISHED ABSTRACTS FROM CONFERENCES: 1. H. N. Kushwaha, N. Gautam, et. al. Effect of carbamazepine- an antiepileptic on pharmacokinetics profile of an antimalarial trioxane in rats. Medicinal Chemistry Research, 19, S68-69, 2010. 2. N. Gautam, H. N. Kushwaha, et. al. Excretion study of a novel antidiabetic S002-853 in rat urine and feces quantified by LC-MS/MS method. Medicinal Chemistry Research, 19, S91-92, 2010. 3. S. K. Amar,S. Gyyal, D. Dubey, H. N. Kushwaha, et. al. Caspase 3 dependent apoptotic cell death induced by Benzophenone-1 through mitochondrial signaling pathway at environmental UVR. Journal of Biomedical and Therapeutics Sciences (ISSN: 2394-2274), 1(1): P-77, 2014 4. S. Goyal, S. K. Amar, A. Dwivedi, D. Chopra, S. F. Mujtaba, H. N. Kushwaha, et. al. Role of Smac/DIABLO in mitochondrial dependent apoptosis by 2-amino-3-hydroxypyridine under ambient UV R exposure. Journal of Biomedical and Therapeutics Sciences (ISSN: 2394- 2274), 1(1): P-93, 2014 MANUSCRIPT COMMUNICATED: 1. R. P. Singh, S. Sabarinath, H. N. Kushwaha, et. al. Comparative pharmacokinetics and oral bioavailability of Racemate and individual stereo-isomers of CDRI-97/78, a new antimalarial agent in monkeys. Drug Research. 2. S. K. Amar, S. Goyal, S.F. Mujtaba, A. Dwivedi, H. N. Kushwaha, D. Chopra, A. verma, P. K. Singh, R. S. Ray. Oxidative stress and DNA damage mediated apoptosis and identification of marker proteins by photosensitized Benzophenone under environmental UV radiation. Archieves of Toxicology. MANUSCRIPT UNDER PREPARATION: 1. H. N. Kushwaha, A. Dwivedi , A. K. Tripathi, et. al. LC-MS/MS cellular uptake analysis revealed photoproduct formation of photoexcited CDRI novel trioxane antimalarial causing type I mediated apoptotic cell death of HaCaT cell line. 5
  • 6. Hari Narayan Kushwaha, M. Pharm. (Gold Medalist), PhD 2. N. Gautam, H. N. Kushwaha, S. Pandey, S. K. Singh. Mass spectrometric development and validation of 99/411 in different tissues and matrices as well as single and multiple dose pharmacokinetics. 3. R. P. Singh, S. Sabarinath, H. N. Kushwaha, et. al. Multiple-dose pharmacokinetics confirm no accumulation and dose proportionality of 97-78, a novel antimalarial agent in Rhesus monkeys. REVIEWS: 1. S. Goyal, S.K. Amar, H.N. Kushwaha, et. al. Toxicological potential of Parabens- a widely used preservative. GJMS. (Accepted November, 2014) BOOK CHAPTER: 5. H.N. Kushwaha, N. Gautam, S. K. Singh. LC-MS/MS assay for quantification of 97/78 and its metabolite 97/63, a promising trioxane antimalarial in human plasma, 11th ISMAS-TRICON- 2009 (ISBN: 978-81-904442-2-4) S.K. Aggrarwal, P.G. Jaison et al. (Editors), p 342-346 (2009). PRESENTATIONS: INVITED LECTURE: 1. Ist Faculty Development Programme (FDP) sponsored by Dr. A.P.J. Abdul Kalam Technical University, Lucknow, from 27.01.2016 to 31.01.2016 at Shri Ram Murti Smarak College of Engg. & Tech. (Pharmacy) Bareilly, U.P. ORAL: 1. A. Dwivedi, S. F. Mujtaba, H. N. Kushwaha, et. al. Photosensitizing action of ofloxacin at ambient UV-A exposure: a mechanistic study. National Seminar on Reactive Oxygen Species: Roles in Animal and Plant Biology, Lucknow University on 23-24 December, 2011. 2. H. N. Kushwaha, A. Khurmi, S. K. Singh. Development and evaluation of nifedipine as transdermal gel. Symposium on Dendrimer in Nano Drug Delivery, CSIR-National Botanical Research Institute, Lucknow on 24-25 December, 2011. POSTERS: 1. H. N. Kushwaha, N. Gautam, S. K. Singh. LC-MS/MS assay for quantification of 97/78 and its metabolite 97/63, a promising trioxane antimalarial in human plasma, 11th ISMAS-TRICON- 2009 November 24-28, 2009, Hyderabad, India. (Best poster) 2. H. N. Kushwaha, N. Gautam, H. Kumar, S.K. Singh. Effect of gabapentin - an antiepileptic on pharmacokinetics profile of 97-78, an antimalarial trioxane in rats, 2nd International Symposium on Drug Metabolism and Pharmacokinetics, Applications toward Drug Discovery and Development, February 2010, NIPER, Punjab, India. 3. H. N. Kushwaha, N. Gautam, S. K. Singh. Effect of carbamazepine- an antiepileptic on pharmacokinetics profile of an antimalarial trioxane in rats. 4th International Symposium on Current Trends in Drug Discovery Research, February 17-21, 2010 at CSIR-CDRI, Lucknow, 6
  • 7. Hari Narayan Kushwaha, M. Pharm. (Gold Medalist), PhD India. 4. N. Gautam, H. N. Kushwaha, S.K. Singh. Excretion study of a novel antidiabetic S002-853 in rat urine and feces quantified by LC-MS/MS method, 4th International Symposium on Current Trends in Drug Discovery Research, February 17-21, 2010 at CSIR-CDRI, Lucknow, India. 5. N. Gautam, H. N. Kushwaha, H. Kumar, R. Pratap, S. K. Singh. Pharmacokinetic and excretion studies of a novel antidiabetic S002-857 in rats. 2nd International Symposium on Drug Metabolism and Pharmacokinetics, Applications toward Drug Discovery and Development, February 2010, NIPER, Punjab, India. 6. N. Gautam, H. Kumar, H. N. Kushwaha, S. K. Singh. Pharmacokinetics, SGF/SIF, and in-situ absorption study of a novel antidiabetic compound S002-857 synthesized by CDRI, 2nd International Symposium on Drug Metabolism and Pharmacokinetics, Applications toward Drug Discovery and Development, February 2010, NIPER, Punjab, India. 7. H. N. Kushwaha, N. Gautam, A. Misra, B. Singh, S. K. Singh. Effect of Phenytoin- an antiepileptic on pharmacokinetics profile of an antimalarial trioxane in rats. 3rd International Symposium on Drug Metabolism and Pharmacokinetics, Applications toward Drug Discovery and Development, February 2011, NIPER, Punjab, India. 8. H. N. Kushwaha, H. H. Siddiqui, S. K. Singh. Comparative pharmacokinetic drug interaction between Gabapentin, an antiepileptic and CDRI-97/78, an antimalarial in male and female rats. AICTE Seminar held at I.T.S. College of Pharmacy, Ghaziabad on 5-6 August, 2011. 9. H. N. Kushwaha, H. Kumar, A. Misra, Y. Singh, S. K. Singh. Pharmacokinetic interaction between CDRI-97/78, novel trioxane antimalarial and Topiramate in male and female SD rats. 4th NIPER (RBL)-CDRI Symposium on Medicinal Chemisrty and Pharmaceutical Sciences at CSIR-Central Drug Research Institute, Lucknow on 23-25 Feburary, 2012. 10. A. Verma, H. N. Kushwaha, M.C. Pant, R.S. Ray. Protection against ultraviolet radiation induced damage in human keratanocytes by oleanolic and ursolic acid. X oint Annual Conference of ISMOCD & IAE, National Institute of Malaria Research Goa Medical College and Hospital, Goa on 12-12 October, 2014 11. S. K. Amar,S. Gyyal, D. Dubey, H. N. Kushwaha, et. al. Caspase 3 dependent apoptotic cell death induced by Benzophenone-1 through mitochondrial signaling pathway at environmental UVR. National Conference on Ethanopharmacology and biotechnology in drug development: Prospects and challenges, Budelkhand University, Jhansi on 14-15 November, 2014. (Best Poster) 12. S. Goyal, S. K. Amar, A. Dwivedi, D. Chopra, S. F. Mujtaba, H. N. Kushwaha, et. al. Role of Smac/DIABLO in mitochondrial dependent apoptosis by 2-amino-3-hydroxypyridine under ambient UV R exposure. National Conference on Ethanopharmacology and biotechnology in drug development: Prospects and challenges, Budelkhand University, Jhansi on 14-15 November, 2014. PERSONAL INFORMATION: Name: Hari Narayan Kushwaha Nationality: Indian (by Birth) Date of birth: 01st May 1980 Place of birth: Durjanpur, Dist. Ballia, Uttar Pradesh, India Gender: Male 7
  • 8. Hari Narayan Kushwaha, M. Pharm. (Gold Medalist), PhD Permanent Address: House at 33A, Near Maurya Caterers, Kanchan Nagar, Mohamadpur Khatri, Kalyanpur, Luknow (U.P.)-226022, India. REFERENCES: 1. Dr. Ratan Singh Ray Principal Scientist and Head, Photobiology Division, CSIR-Indian Institute of Toxicology Research, Lucknow -226 001 (U.P.) India. Mobile No.: 09935882991 E-mail: ratanray.2013@rediffmail.com 2. Dr. S. K. SINGH Pharmacokinetics And Metabolism Division, CSIR-Central Drug Research Institute, Lucknow -226 001 (U.P.) India, Mobile No.: 0919450360338, E-mail: shiokumar@yahoo.com. : DECLARATION: I hereby declare that the above information is factually correct to the best of my knowledge. I also understand that I will be disqualified if any of the information is found to be wrong. Date: Place: Greater Noida ------------------------------------ Signature of the Candidate 8