The curriculum vitae outlines the professional experience and qualifications of Polagani Srinivasa Rao, who has over 11 years of experience developing and validating analytical methods using LC-MS/MS to quantify pharmaceutical drugs, metabolites, and other compounds in biological samples under GLP and FDA regulations. He currently works as a Manager of Bioanalytical Research at Indoco Remides, where he leads projects involving method development, validation, sample preparation, and regulatory compliance. Rao also has publications in peer-reviewed journals and presents technical information at meetings and seminars.

1. Curriculum Vitae
Polagani Srinivasa Rao,
E-mail: srinu_polagani@yahoo.co.in
Hyderabad,
Contact No: 9700693699
Objective:
Seeking quality environment where my knowledge and experience can be shared.
Accomplished, results-oriented professional interested in working in a bio-
analytical/analytical research field. Expert in the qualitative and quantitative analysis
of drugs and metabolites and NCEs using mass spectrometric techniques.
Demonstrated experience in chromatographic method development/validation for
pharmaceutical drugs, drug intermediates, under GLP/FDA regulations.
I am a highly motivated bioanalytical scientist with hands on experience in
research and managing complex interdisciplinary team projects focused on proteomic
methods and technology development for research in life sciences. My research
involves the development of novel bioanalytical methods for the determination of
pharmaceutical drugs such using LC-MS/MS and also acquired special skills in sample
preparation of biomolecules. I successfully designed and conducted various analytical
experiments and validated for the biomolecules for a pharmacokinetic/bioequivalence
studies.
Experience:
• Presently I am working with Indoco Remides (Previously its known as Piramal
Clinical Research a division of Piramal Healthcare Limited) as a Manager-
Bioanalytical research (January 2007-till date). Previously worked with Vimta Labs
(August 2004-January 2007).
• Outstanding 11 years track record of successful experiences in developing and
applying the state-of-the-art to identify, and quantify pharmaceutical drugs and their
metabolites in the biological samples.
• Hands on experience in DMPK studies and developing an analytical method for
NCEs.
• As a technical expert I regularly use specialized analytical techniques (ABI/SCIEX
4000/3000/2000) for quantitation of drugs and metabolites in biological samples.
• A strong background in chromatographic method development of various biological
compounds. Provided support for new LC/MS system installation, preventative
maintenance, troubleshooting and minor repairs of analytical scientific equipments.
• Additional expertise in biological sample preparation (from tissues, serum and
plasma), various extraction methods such as protein purification, and liquid-liquid
extraction or solid phase extraction.

2. Educational Qualifications: M.Sc Organic Chemistry with from Andhra
University, B.Sc (M.P.C) with from Kakatiya
University.
Skills:
• Applied analytical method validation for new market/research products and
successfully handled the regulatory audits like GLP and US-FDA.
• Developed and validated sensitive and selective analytical methods for pharma
drug products and metabolites using HPLC and HPLC-MS instrumental techniques.
• Presented technical information about assigned projects at meetings and seminars
and obtained theoretical and practical understanding of the full range of drug
development process.
• Contributed to the implementation of reviewing and verifying data in laboratory
notebooks and technical reports. Supervise hiring, training and coaching of junior
chemists.
• Maintained and enforced applicable government regulatory, safety, occupational
health, and environmental requirements.
• Provided updates to production/QA regarding progress and issues. Ensured that
projects were conducted under GLP guidelines and the FDA guidance and were
correctly documented for global submission.
• Prioritized workloads and maintained a strong internal and external network.
Demonstration of enthusiasm, creativity, initiative, problem-solving skills, track
record of tangible productivity, significant contribution to the team and company
goals.
• Writing day-to-day analytical reports/lab manuals/protocols and co-coordinating
with production/QA. Involved in reviewing technical documents and effectively
manage both internal and external resources assigned to my project(s).
• Established strong working relationship with analytical development and other
cross-functional groups with in and outside the company.
Additional Information:
PUBLICATION LIST
1. Simultaneous determination of atorvastatin and aspirin in human plasma by
LC-MS/MS with positive/negative polarity switch and its application to a
human pharmacokinetic study. Ramakrishna Gajula, Vasu Babu Ravi,
Srinivasa Rao Polagani, Rambabu Maddela, Sobha Busa, Jaswanth Kumar
Inamadugu, Nageswara Rao Pilli*(as a corresponding author). Scientia
Pharmaceutica (2012) DOI: 10.3797/scipharm.1206-12.
2. A rapid and sensitive liquid chromatography–tandem mass spectrometric
assay for cycloserine in 50 µL of human plasma: Its pharmacokinetic
application. Srinivasa Rao Polagani, Nageswara Rao Pilli, Rambabu

3. Maddela, Ramakrishna Gajula, Venkateswarlu Gandu. Journal of
Pharmaceutical and Biomedical Analysis (Accepted on 26th
Dec 2012).
3. Simultaneous determination of atorvastatin, metformin and glimepiride in
human plasma by LC–MS/MS and its application to a human pharmacokinetic
study. Srinivasa Rao Polagani, Nageswara Rao Pilli, Ramakrishna Gajula,
Venkateswarlu Gandu. Journal of Pharmaceutical Analysis, (2012),
doi.org/10.1016/j.jpha.2012.09.002.
4. Simultaneous Determination of Ritonavir and Lopinavir in Human Plasma after
Protein Precipitation and LC-MS-MS. Rajasekhar Damaramadugu,
Jaswanthkumar Inamadugu, Ravi Kanneti, Srinivasulu Polagani,
Venkateswarlu Ponneri. Chromatographia May 2010, Volume 71, Issue 9-10,
pp 815-824.( DOI 10.1365/s10337-010-1550-9)
5. Liquid chromatography–tandem mass spectrometric assay for the light
sensitive calcium channel antagonist lacidipine in human plasma. Vijaya
Kumari Karraa
, Nageswara Rao Pillia
, Srinivasa Rao Polaganib
, Jaswanth
Kumar Inamaduguc
, J.V.L.N. Seshagiri Raod
. Journal of Pharmaceutical and
Biomedical Analysis.Volume 66, July 2012, Pages 211–
217(http://dx.doi.org/10.1016/j.jpba.2012.02.022, How to Cite or Link Using
DOI)
6. High performance liquid chromatography mass spectrometric method for the
simultaneous quantification of pravastatin and aspirin in human plasma:
Pharmacokinetic application.Srinivasa Rao Polagania,
, Nageswara Rao
Pillib
, Venkateswarlu Gandu. Journal of Pharmaceutical Analysis.Volume 2,
Issue3,June2012,Pages206–213.DOI
http://dx.doi.org/10.1016/j.jpha.2012.01.001,
7. Rapid liquid chromatographic -tandem mass spectrometric method
for the quantification of pravastatin in human plasma. Nageswara Rao Pilli1,2,
Jaswanth Kumar Inamadugu2, Vijaya Kumari Karra1, SrinivasaRao
Polagani2 and J. V. L. N. Seshagiri Rao3* Scholars Research LibraryDer
Pharmacia Lettre, 2012, 4 (1):142-151
8. A rapid and sensitive liquid chromatography-tandem mass spectrometric
assay for the montelukast in human plasma: A pharmacokinetic study. 7th
National conference on “Thermodynamic of chemical, biological and
environmental processes”srivenkateswara university tirupati.
Personal profile:
Fathers Name : NAGESWARARAO
Date of Birth : 18th
August 1979
Languages known : Telugu, English & Hindi
Declaration
I here by declare that the above furnished information is true to the best of my
knowledge.
(Polagani Srinivasa Rao)