Evaluation of the safety of conventional lighting replacement by artificial d...Prof. Hesham N. Mustafa
Background
Short morning exposure to high illuminance visible electromagnetic radiations termed as artificial daylight is beneficial for the mental health of people living in geographical areas with important seasonal changes in daylight illuminance. However, the commercial success of high illuminance light sources has raised the question of the safety of long hour exposure.
Methods
We have investigated the effect of the replacement of natural daylight by artificial daylight in Swiss mice raised under natural lighting conditions. Mice were monitored for neurotoxicity and general health changes. They were submitted to a battery of conventional tests for mood, motor and cognitive functions’ assessment on exposure day (ED) 14 and ED20. Following sacrifice on ED21 due to marked signs of neurotoxicity, the expression of markers of inflammation and apoptosis was assessed in the entorhinal cortex and neurons were estimated in the hippocampal formation.
Results
Signs of severe cognitive and motor impairments, mood disorders, and hepatotoxicity were observed in animals exposed to artificial daylight on ED20, unlike on ED14 and unlike groups exposed to natural daylight or conventional lighting. Activated microglia and astrocytes were observed in the entorhinal cortex, as well as dead and dying neurons. Neuronal counts revealed massive neuronal loss in the hippocampal formation.
Conclusions
These results suggest that long hour exposure to high illuminance visible electromagnetic radiations induced severe alterations in brain function and general health in mice partly mediated by damages to the neocortex-entorhinal cortex-hippocampus axis. These findings raise caution over long hour use of high illuminance artificial light.
The International Large Detector (ILD) is a concept for a detector at the International
Linear Collider, ILC. The ILC will collide electrons and positrons at energies of initially
500 GeV, upgradeable to 1 TeV. The ILC has an ambitious physics program, which will
extend and complement that of the Large Hadron Collider (LHC). The ILC physics case
has been well documented, most recently in the ILC Reference Design Report, RDR [1]. A
hallmark of physics at the ILC is precision. The clean initial state and the comparatively
benign environment of a lepton collider are ideally suited to high precision measurements.
To take full advantage of the physics potential of ILC places great demands on the detector
performance. The design of ILD, which is based on the GLD [2] and the LDC [3] detector
concepts, is driven by these requirements. Excellent calorimetry and tracking are combined to
obtain the best possible overall event reconstruction, including the capability to reconstruct
individual particles within jets for particle flow calorimetry. This requires excellent spatial
resolution for all detector systems. A highly granular calorimeter system is combined with a
central tracker which stresses redundancy and efficiency. In addition, efficient reconstruction
of secondary vertices and excellent momentum resolution for charged particles are essential
for an ILC detector. The interaction region of the ILC is designed to host two detectors,
which can be moved into the beam position with a “push-pull” scheme. The mechanical
design of ILD and the overall integration of subdetectors takes these operational conditions
into account. The main features of ILD are outlined in the present document.
Dedicated Research Scientist with experience in bacteriology and virology research and patient service. . Possessing excellent communication skills and able to work as a part of a team, with proven ability to work in tight schedules and adherence to deadlines. I grew keen interest in viral research during this period. Two research articles have been prepared by me during this period. One manuscript entitled," Prevalence of Hepatitis A and Hepatitis E in West Bengal, India: a tertiary care hospital based study" has been accepted in JEMDS and will be published in next issue.
The second one which is on dengue intervention is under consideration for publication in a very reputed journal.
My Ph.D. is on biomedical device associated biofilm infections where I have studied incidence, antibiotic tolerance of bacteria within biofilm and tried to find out some intervention strategies. I have published seven original research article in this topic and few others are in queue.
Bacteria within biofilm shows extremely high resistance towards antimicrobials used than its free floating counterparts (approx. 1000 fold). I would like to investigate the causes of this high resistance.
Biomaterials for medical implantation research strategies - PubricaPubrica
Biomaterials and medical instruments are widely researched and incorporated, which greatly increase the quality of human life, thanks to the rapid advancement of biomedical science and practice
Continue Reading: https://bit.ly/2UOpyqw
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Email: sales@pubrica.com
WhatsApp : +91 9884350006
United Kingdom: +44-1618186353
Evaluation of the safety of conventional lighting replacement by artificial d...Prof. Hesham N. Mustafa
Background
Short morning exposure to high illuminance visible electromagnetic radiations termed as artificial daylight is beneficial for the mental health of people living in geographical areas with important seasonal changes in daylight illuminance. However, the commercial success of high illuminance light sources has raised the question of the safety of long hour exposure.
Methods
We have investigated the effect of the replacement of natural daylight by artificial daylight in Swiss mice raised under natural lighting conditions. Mice were monitored for neurotoxicity and general health changes. They were submitted to a battery of conventional tests for mood, motor and cognitive functions’ assessment on exposure day (ED) 14 and ED20. Following sacrifice on ED21 due to marked signs of neurotoxicity, the expression of markers of inflammation and apoptosis was assessed in the entorhinal cortex and neurons were estimated in the hippocampal formation.
Results
Signs of severe cognitive and motor impairments, mood disorders, and hepatotoxicity were observed in animals exposed to artificial daylight on ED20, unlike on ED14 and unlike groups exposed to natural daylight or conventional lighting. Activated microglia and astrocytes were observed in the entorhinal cortex, as well as dead and dying neurons. Neuronal counts revealed massive neuronal loss in the hippocampal formation.
Conclusions
These results suggest that long hour exposure to high illuminance visible electromagnetic radiations induced severe alterations in brain function and general health in mice partly mediated by damages to the neocortex-entorhinal cortex-hippocampus axis. These findings raise caution over long hour use of high illuminance artificial light.
The International Large Detector (ILD) is a concept for a detector at the International
Linear Collider, ILC. The ILC will collide electrons and positrons at energies of initially
500 GeV, upgradeable to 1 TeV. The ILC has an ambitious physics program, which will
extend and complement that of the Large Hadron Collider (LHC). The ILC physics case
has been well documented, most recently in the ILC Reference Design Report, RDR [1]. A
hallmark of physics at the ILC is precision. The clean initial state and the comparatively
benign environment of a lepton collider are ideally suited to high precision measurements.
To take full advantage of the physics potential of ILC places great demands on the detector
performance. The design of ILD, which is based on the GLD [2] and the LDC [3] detector
concepts, is driven by these requirements. Excellent calorimetry and tracking are combined to
obtain the best possible overall event reconstruction, including the capability to reconstruct
individual particles within jets for particle flow calorimetry. This requires excellent spatial
resolution for all detector systems. A highly granular calorimeter system is combined with a
central tracker which stresses redundancy and efficiency. In addition, efficient reconstruction
of secondary vertices and excellent momentum resolution for charged particles are essential
for an ILC detector. The interaction region of the ILC is designed to host two detectors,
which can be moved into the beam position with a “push-pull” scheme. The mechanical
design of ILD and the overall integration of subdetectors takes these operational conditions
into account. The main features of ILD are outlined in the present document.
Dedicated Research Scientist with experience in bacteriology and virology research and patient service. . Possessing excellent communication skills and able to work as a part of a team, with proven ability to work in tight schedules and adherence to deadlines. I grew keen interest in viral research during this period. Two research articles have been prepared by me during this period. One manuscript entitled," Prevalence of Hepatitis A and Hepatitis E in West Bengal, India: a tertiary care hospital based study" has been accepted in JEMDS and will be published in next issue.
The second one which is on dengue intervention is under consideration for publication in a very reputed journal.
My Ph.D. is on biomedical device associated biofilm infections where I have studied incidence, antibiotic tolerance of bacteria within biofilm and tried to find out some intervention strategies. I have published seven original research article in this topic and few others are in queue.
Bacteria within biofilm shows extremely high resistance towards antimicrobials used than its free floating counterparts (approx. 1000 fold). I would like to investigate the causes of this high resistance.
Biomaterials for medical implantation research strategies - PubricaPubrica
Biomaterials and medical instruments are widely researched and incorporated, which greatly increase the quality of human life, thanks to the rapid advancement of biomedical science and practice
Continue Reading: https://bit.ly/2UOpyqw
For our services: https://pubrica.com/sevices/research-services/
Why Pubrica:
When you order our services, We promise you the following – Plagiarism free | always on Time | 24*7 customer support | Written to international Standard | Unlimited Revisions support | Medical writing Expert | Publication Support | Biostatistical experts | High-quality Subject Matter Experts.
Contact us:
Web: https://pubrica.com/
Blog: https://pubrica.com/academy/
Email: sales@pubrica.com
WhatsApp : +91 9884350006
United Kingdom: +44-1618186353
The Topic is Radioprotective Efficacy of RK-IP-006 in mammalian system. Experiments performed were Antioxidant assay, SDS-PAGE, Western Blot to check the effect against radiation of 9Gy.
Gujarat Vibrant - International Conference on human GeneticsVibrant Gujarat
The conference was aimed to bring together internationally and nationally renowned scientists to discuss the latest developments, trends, technologies and clinical applications in various areas of human genetics.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
Comparative structure of adrenal gland in vertebrates
Manish adhikari Resume
1. 1
CURRICULUM VITAE
Dr. MANISH ADHIKARI
Postdoctoral Scientist
School of Engineering&AppliedSciences,
The George WashingtonUniversity
Science &EngineeringHall 3582
800 22nd
Street,Northwest
Washington,DC20052, USA
Phone:+1 (301)(728)(8296)
E-mail:manishadhikari85@gmail.com;mani85phd@gwu.edu
PERSONAL PROFILE
Name : MANISH ADHIKARI
Date of Birth : 03 Apr 1985
Father’sName : Dr. J. S. Adhikari
Sex : Male
Languages Known : English,Hindi
Address : 1001 Rockville PikeApt602
Rockville,MD20852, USA
EDUCATION
- 2019 to till present– Postdoctoral Scientist:Departmentof Mechanical & Aerospace
Engineering,The George WashingtonUniversity,WashingtonDC,USA.
- 2017 to 2019 – Research AssistantProfessor: PlasmaBioscience ResearchCentre,
KwangwoonUniversity,Seoul,Republicof SouthKorea.
- 2016 to 2017 – Postdoctoral Researcher:PlasmaBioscience ResearchCentre,Kwangwoon
University,Seoul,Republicof SouthKorea.
- 2014 to 2016 – Research Associate:Institute of NuclearMedicine &AlledSciences,Defence
Research & Development Organisation (DRDO), Delhi, India.
- 2014 to 2015 – Project Research Fellow: “Marie Curie Actions People International
Research Staff Exchange Scheme” at Faculty of Medicine, Trakia University, Stara Zagora,
Bulgaria.
- 2008 to 2014 – Ph.D/Doctorate (Life Sciences): Bharathiar University, Coimbatore, India.
Thesis Title: ‘Evaluation of selected natural products for radioprotective efficacy’.
- 2005 to 2007 – M.Sc. (Biomedical Sciences): Department of Biomedical Sciences,
Bundelkhand University, Jhansi, Uttar Pradesh, India.
- 2002 to 2005 – B.Sc. (Biology) : University of Delhi, Delhi, India.
2. 2
TECHNICAL SKILLS & TRAINING
More than 5.5 years (in total) of research experience (Post-PhD) in advanced Molecular
biology, Cancer biology & versatile knowledge and hands-on experiences in
multidisciplinaryareaslike molecularbiology, cell biology, protein expression in different
expression systems.
More than 3.5 years research experience in cold plasma treatment using different plasma
sources and in hand experience in maintaining and culturing various cancer cell lines.
Thoroughknowledge and in-handexpereinece offlowcytometerand did certificate course
on BD LSR II flow cytometer at BD FACSOrient course at BD-Jamia Hamdard University.
Ability to handle various research projects from the feasibility to the implementation
stage.
Focused and hardworking professional equipped with thorough knowledge and technical
understanding
Well versed with both, cell lines and animal models (Mice, Rats).
Well versed with routine molecular biology techniques like flow cytometry, cellular and
molecularimaging,westernblotting,cometassay, fluorescentmicroscopy,q-PCR, UV visible
spectroscopy etc.
PROJECTS HANDLED
“Plasma Bioscience and Medicine” Science Research Center (SRC) Project under National
Research Foundation of Korea, South Korea
“Studies on some herbal additives giving partial protection against toxic or
immunosuppressive effects of some mycotoxins and improving wound granulation” under
European Union’s Marie Curie International Research Staff Exchange Scheme.
“Equipment in the Electron paramagnetic resonance centre with the new apparatus,
necessary for investigation '' real time '' oxidative status of animals treated alone and in
combination with Indian and African antioxidants as natural protectors against chronic
mycotoxicity”underCompetitionForResearchProjects (Grant:- Ministryof Education, Sofia,
Bulgaria) 2015; Project number 6/ 2015 - 14000 lv.
“Evaluation of Selected Natural Products for their Radio-Protective Efficacy” at Nuclear
Medicine & Alled Sciences, Defence Research & Development Organisation, Delhi, India.
“Studies on Radio-Resistive Properties of Novel Bacterium, Isolated from Radioactive
Environment:Implication in Radioprotection” atNuclearMedicine &AlledSciences,Defence
Research & Development Organisation, Delhi, India.
PUBLICATIONS (in refereed journals)
1. Bhawana Adhikari*
, Manish Adhikari*
, Bhagirath Ghimire, Bishwa Chandra Adhikari,
Gyungsoon Park and Eun Ha Choi. Cold plasma seed priming modulates the growth, redox
homeostasis and stress response of tomato seedlings. Free Radical Biology & Medicine
(Communicated) (Co-first author).
2. Dayun Yan*
, Qihui Wang*
, Manish Adhikari*
, Denis B Zolotukhin , Xiaoliang Yao , Li Lin ,
Megan Kirschner , Jonathan H Sherman , Michael Keidar. Cold Atmospheric Plasma Action
on MelanomaCells:A NewFormof Cell Death.AdvancedScience (Communicated) (Co-first
3. 3
author).
3. Manish Adhikari, Bhawana Adhikari, Bhagirath Ghimire, Sanjula Baboota and Eun Ha Choi.
Cold atmospheric plasma and silymarin nanoemulsion activate Autophagy in human
melanoma cells. (2020). International Journal of Molecular Sciences, 21(6), 1939.
4. DharmendraKumarYadav*
, Manish Adhikari*
, SurendraKumar,BhagirathGhimire,Mi-Hyun
Kimand Eun-HaChoi.Coldatmosphericplasmageneratedreactive species aided inhibitory
effects on human melanoma cells: an in vitro and in silico study. (Co-first author) (2020).
Scientific Reports 10 (1), 1-15.
5. Manish Adhikari, Bhawana Adhikari, Anupriya Adhikari, Dayun Yan, Vikas Soni, Jonathan
Sherman and Michael Keidar. Cold atmospheric Plasma as a bovel therapeutic tool for the
treatment of brain cancer. (2020). Current Pharmaceutical Design [Epub ahead of print].
doi: 10.2174/1381612826666200302105715.
6. Sohail Mumtaz,PradeepBhartiya,NehaKaushik, Manish Adhikari et al. Pulsed high-power
microwavesdonotimpairthe functionsof skinnormal andcancer cellsin vitro: a short-term
biological evaluation. (2020). Journal of Advanced Research 22, 47-55.
7. Manish Adhikari, Bhawana Adhikari, Neha Kaushik, Su-Jae Lee, Nagendra Kumar Kaushik
and Eun Ha Choi. Melanoma growth analysis in blood serum and tissue using xenograft
model with response to cold atmospheric plasma activated medium. (2019). Applied
Sciences 9 (20), 4227.
8. Bhawana Adhikari*
, Manish Adhikari*
, Bhagirath Ghimire, Gyung Soon Park and Eun Ha
Choi. Cold Atmospheric Plasma Activated Water Irrigation Induces Defense Response in
Tomato Seedlings. (Co-first author) (2019). Scientific Reports 9 (1), 1-15.
9. NagendraKumarKaushik,NehaKaushik, ManishAdhikari,BhagirathGhimire, Nguyen Nhat
Linh, Yogendra Kumar Mishra, Su Jae Lee, Eun Ha Choi. Preventing the solid cancer
progression via release of anticancer-cytokines in co-culture with cold plasma-stimulated
macrophages. (2019). Cancers. 11 (6) 842.
10. Manish Adhikari, Neha Kaushik, Bhagirath Ghimire, Bhawana Adhikari, Sanjula Baboota,
AbdulazizA Al-Khedhairy,Rizwan Wahab, Su-Jae Lee, Nagendra Kumar Kaushik and Eun Ha
Choi. Coldatmosphericplasma and silymarin nanoemulsion synergistically inhibits human
melanoma tumorigenesis via targeting HGF/c-MET downstream pathway. (2019). Cell
Communication and Signaling. 17 (1) 52.
11. Nagendra Kumar Kaushik, Bhagirath Ghimire, Ying Li, Manish Adhikari, Mayura Veerana,
Neha Kaushik, Nayansi Jha, Bhawana Adhikari, Eun Ha Choi. Biological and medical
application of plasma activated media, water and solutions. (2018). Biological Chemistry.
400 (1): 39-62.
12. Yanka Karamalakova, Galina Nikolova, Manish Adhikari, Stoycho Stoev, Prerna Agarwal,
VeselinaGadjeva,ZhivkoZhelev.Oxidative-protective effectsof Tinospora cordifolia extract
on plasmaandspleencells after experimental ochratoxicosis. (2018). Comparative Clinical
Pathology. 27(6): 1487-1495.
13. Anupriya Adhikari, Neelam Kumari, Manish Adhikari, Nitin Kumar et al. Zinc complex of
tryptophan appended 1,4,7,10-tetraazacyclododecane as potential anticancer agent:
Synthesis and evaluation. (2017). Bioorganic & Medicinal Chemistry 25 (13): 3483-3490.
14. Manish Adhikari, Bhawana Negi, Neha Kaushik, Anupriya Adhikari et al. T-2 Mycotoxin:
Toxicological Effects and Decontamination Strategies. (2017). Oncotarget 8 (20): 33933-
33952.
15. Poonam Malhotra, Manish Adhikari, Saurabh Mishra et al. N-acetyl Tryptophan-
Glucopyranoside (NATG) as a countermeasure against gamma-radiation-induced immune-
suppression in murine macrophage J774A.1 cells. (2016). Free Radical Research 50 (11):
1265-1278.
4. 4
16. Yanka Karamalakova, Prerna Agarwal, Galina Nikolova, Manish Adhikari, Damodar Gupta,
StoychoStoevetal. Influenceof Ochratoxin-A andanextractof Tinospora Cordifolia against
Biochemical and Oxidative changes in mice spleen. (2016). Science & Technologies 6: 242-
251.
17. PrernaAgarwal,YankaKaramalakova, Manish Adhikari, Damodar Gupta et al. Investigation
on DPPH scavenging capacity before and after UV-irradiation of aqueous root extract of
Glycyrrhiza glabra. (2015). Journal of Bioscience and Biotechnology (Special Ed) 183-188.
18. Manish Adhikari, Rajesh Arora. Nano-silymarin provides protection against γ-radiation-
induced oxidative stress in cultured human embryonic kidney cells. (2015). Mutation
Research - Genetic Toxicology and Environmental Mutagenesis. 792: 1-11.
19. Poonam Malhotra, Manish Adhikari, Shravan K Singh, Raj Kumar. N-Acetyl Tryptophan-
Glucopyranoside (NATG) provides Radioprotection to Murine Macrophages J774A.1 cells.
(2015). Free Radical Research 49 (12): 1488-1498.
20. Manish Adhikari, Rajesh Arora. The Flavonolignan-Silymarin Protects Enzymatic,
Hematological, and Immune System Against -Radiation-Induced Toxicity. (2014).
Environmental Toxicology 31 (6) 641-654.
21. Anupriya Adhikari, Anupama Datta, Manish Adhikari et al. Preclinical Evaluation of DO3A-
Act-AQ:A Polyazamacrocyclic Monomeric Anthraquinone Derivative as Theranostic Agent.
(2014). Molecular Pharmaceutics, 11(2): 445-456.
22. Rajesh Arora, Manish Adhikari, et al Amelioration of -radiation-induced genotoxicity by
Nanosilymarin:A Comparative studyindicatespossible implications for chemical biological
radiological and nuclear (CBRN) defence. (2014). Trakia Journal of Sciences 12 Suppl (1): 1-
10.
23. Manish Adhikari, et al. In vitro studies on radioprotective efficacy of silymarin against -
irradiation. (2013). International Journal of Radiation Biology 89(3): 200-211.
24. Manish Adhikari,etal. A comparative evaluationof anantioxidantof natural origin derived
from Silybum marianum characterized by in vitro assays and electron paramagnetic
resonance spectroscopy. (2012). Trakia Journal of Sciences 10 Suppl (1): 17-24.
25. Manish Adhikari, et al. Evaluation of Silymarin as a Promising Radioprotector. (2010).
Zeitschrift für Naturforsch C (Max-Planck Institute Journal) 65c, 337-346.
26. Rajesh Arora, Raman Chawla, Atlar Singh Dhaker, Manish Adhikari, et al. Podophyllum
hexandrum as a Potential Botanical Supplement for the Medical Management of Nuclear
and Radiological Emergencies (NREs) and Free Radical-Mediated Ailments: Leads From In
Vitro/In Vivo Radioprotective Efficacy Evaluation. (2010). Journal of Dietary Supplement
7(1): 31-50.
27. Rajesh Arora, Raman Chawla, Atlar Singh Dhaker, Manish Adhikari, et al. Pro-Antioxidant
Activities of Fractions of a Novel Camptothecin-producing Endophyte (Entrophosphora
infrequens). (2010). Trakia Journal of Sciences 8(2): 1-15.
Book Chapter
1. Manish Adhikari, Anser Ali, Nagendra Kaushik, Eun Ha Choi. Perspective in Pigmentation
Disorders. (2018). Comprehensive Clinical Plasma Medicine, Springer International
Publishing, Ed. HR Metelmann, pp 363-400.
2. Rajesh Arora, A.S. Dhaker, Manish Adhikari et al. Strengthening the Armamentarum of
Indian Defence Forces for Radiological/ Nuclear Defence Preparedness: Potential of
Radiation Countermeasure Agents of Natural Origins. (2008). Life Science Pespective,
Macmillan publishers India Ltd. Ed. Manas K. Mandal. pp 72-96.
5. 5
Reviewer (List of Journals)
1. Plos One
2. Scientific Reports (Nature Publishing House, UK)
3. Oxidative Medicine & Cellular Longevity (Hindawi)
4. MDPI Cancers
5. MDPI Coatings
6. MDPI International Journal of Molecular Sciences
7. MDPI Plasma
8. Journal of Complementary and Integrative Medicine (De Gruyter)
9. International Journal of Biological Macromolecules (Elsevier)
10. Journal of Biomolecular Structure and Dynamics (Taylor & Francis)
11. Materials Today Chemistry (Elsevier)
ABSTRACTS AND SHORT COMMUNICATIONS
1. Manish Adhikari, Vikas Soni, Simonyan Hayk, Colin Young, Jonathan Sherman and Michael
Keidar.Combinationtreatmentof iocompatible coldplasmaandchemotherapeuticagent to
sensitize humanglioblastoma. 8th
International Conference on Plasma Medicine (ICPM-8),
Songdo Convensia, Incheon, Korea (14 – 19 June 2020).
2. Manish Adhikari, Vikas Soni, Simonyan Hayk, Colin Young, Jonathan Sherman and Michael
Keidar. Co-effect of He-Plasma and TMZ in regression of intracranial glioblastoma
multiforme model. 7th
International Workshop on Plasma for Cancer Treatment (IWPCT
2020), NC State University, Raleigh, North Carolina, USA (23 – 25 March 2020).
3. NagendraKaushik,NehaKaushik,NguyenNhatLinh,PradeepBhartiya, ManishAdhikari and
Bhagirath Ghimire. International Symposium for Plasma Biosciences (ISPB2019-9). St.
John’s Hotel (4F), Gangneung City, Korea (23 – 26 June 2019).
4. Eun Ha Choi, Manish Adhikari and Nagendra Kaushik. Interactions of functionalized
nanoparticles with Nonthermal Biocompatible Plasma (NBP) sources. The 8th
Advanced
Functional Materials & Devices (AFMD 2018), Catholic University of Leuven, Leuven,
Belgium (17-19 August 2018).
5. Manish Adhikari,NehaKaushik,SuJae Lee,NagendraKaushikand Eun Ha Choi. Anti-cancer
synergistic effect of Self Nano Emulsifying Drug Delivery System (SNEDDS) with cold
atmosphericplasma.7thInternational Conference on Plasma Medicine, Drexel University,
Philadelphia, USA (2018).
6. Nagendra Kaushik, Neha Kaushik, Manish Adhikari, Su-Jae Lee and Eun Ha Choi. Immuno-
modulatoryeffectof bio-plasmaagainst cancer and other dreadful disease. Clinical Plasma
Medicine, Elsevier (2018).
7. VerginaMateva,Yana Karamalakova,Prernaagarwal, ManishAdhikari and Galina Nikolova.
Immuno-modulatory effects of Curcuma longa L. extract against Ochratoxin (A)- induced
reactive oxygenspeciesandimmuneresponse. Leiden International (Bio) Medical Student
Conference, Leiden University Medical Center, The Netherland (2017).
8. NagendraKaushik,NehaKaushik, ManishAdhikari,EunHa Choi.Immuno-modulationeffect
of bio-plasma and its application in Cancer treatment. 1st
Joint workshop of INP-PBRC
Applied Plasma Medicine Centre (APMC), Kwangwoon University, Seoul, South Korea
(2017).
9. Shravan Kumar Singh, Manish Adhikari, Poonam Malhotra, Ashutosh Kumar Gupta, Neha
Chachhia, Darshana Singh and Raj Kumar. Proteomics and Bioinformaticsanalysis of
molecular mechanismsof Radioprotection offered by Radioresistant Bacterial Metabolite
RKIP-006. Journal of Proteins and Proteomics (Special Issue); 7, (4) (2016).
6. 6
10. Rajesh Arora, Damodar Gupta, Raman Chawla, Prerna Agarwal, Manish Adhikari et al.
Impact of climate change on mycotoxins in food: Management interventions by herbs of
Indian, European & South African origin. National seminar on “Challenges of climate
change and green environmental solutions”, Chaudhary Charan Singh University, Meerut,
India (2016).
11. Manish Adhikari, Rajesh Arora, Yana Karamalakova, Raj Kumar et al. -radiation induced
DNA damage attenuation by Nano-silymarin: An in vitro Approach. 70 years anniversaru
Scientific conference, Medical University of Plovdiv, Plovdiv, Bulgaria (2015).
12. Manish Adhikari, Yana Karamalakova, Veselin Ivanov, Damodar Gupta et al. Evaluation of
Silymarin as a Prospective Countermeasure against Radiation and Mycotoxin-induced
Toxicity. Medical conference, Stara Zagora, Bulgaria (2015).
13. Manish Adhikari,Yana Karamalakova,GalinaNikolova,DamodarGuptaetal. Nanosilymarin
as an antioxidantagent:Comparative in vitro studies. XIV Internatinal Congress of Medical
Sciences, Sofia, Bulgaria (2015).
14. Rajesh Arora, Manish Adhikari et al. Nanosilymarin as an effective radiation and biothreat
countermeasure agent: evidence from in vitro and in vivo studies. 1st
Trakia Medical Days,
International Scientific Conference, Stara Zagora Bulgaria (2014).
15. Manish Adhikari,Yanka Karamalakova,Veselin Ivanov, Antonie Zheleva, Veselina Gadjeva
and Rajesh Arora. Silymarin as a Potent Mitigator for Handling Radiological Emergencies.
Medical Conference, Stara Zagora, Bulgaria (2013).
16. Manish Adhikari, Veselin Ivanov, Atlar singh Dhaker, Kamini Bharti, Sanjula Baboota,
Veselina Gadjeva, Yanka Karamalakova, Roop Kumar Khar, Raman Chawla, Rakesh Kumar
Sharma and Rajesh Arora. Development, characterization and evaluation of silymarin
nanoemulsion as radioprotector. Bio Active Substances and Materials 2013 (BAMS 2013),
Novy Svet, Crimea, Ukraine. (2013).
17. Manish Adhikari et al. Silymarin can be used as a safe radiation countermeasure in the
eventof radiological emergencies:Evidence frominvitroandinvivostudies.(2012). Journal
of Cancer Research and Therapeutics, 8 (3), 487.
18. Jyoti Sharma, Manish Adhikari et al. Upregulation of Endogenous Antioxidant System by
Dietary Ingredient IBG15 Results in Mitigation of lonizing Radiation-Induced Conditioned
Taste Aversion (CTA) in Sprague Dawley Rats. (2010). Advanced Biotechnology, 10(5), 115.
19. Manish Adhikari,etal. The Radioprotective Properties of Silymarin for the Management of
Radiological Incidents. (2010). Advanced Biotechnology, 10(5) 128.
20. Poonam Malhotra, Manish Adhikari, et al. Optimizing Radioprotective Efficacy of a
Fractionated Extract of Podophyllum hexandrum for Mitigation of Radiation-Induced Free
Radical-MediatedBiological Effectsinthe Hemopoietic Syndrome Range. (2010). Advanced
Biotechnology, 10(5), 128.
21. Atlar Singh Dhaker, Manish Adhikari et al. EPR Spectroscopy Confirms Modification of
Oxidative Stress by Psoralea corylifolia lin. as the Mechanism of Radioprotection. (2010),
Advanced Biotechnology, 10(5), 129.
22. Atlar Singh Dhaker, Rajesh Arora, Manish Adhikari, et al. Study on the aqueous extract of
INM-2008 as a potent antioxidant and putative radio protector. (2008). Indian Journal of
Radiation Research, 5(3-4), 85.
23. Rajesh Arora, Atlar Singh Dhaker, Raman Chawla, Damodar Gupta, Manish Adhikari et al.
Critical issuesindevelopmentof novel radiation countermeasures from natural sources for
nuclearand radiological emergencies. (2008). Indian Journal of Radiation Research, 5(3-4),
108.
7. 7
24. RajeshArora,Raman Chawla,SatishChandraPuri,AtlarSinghDhaker, ManishAdhikari et al.
Fractionation, isolation, and chemical characterization of 3-O-β-D galactopyransoside of
quercetinformPodophyllumhexadrumRoyle andevaluationof itsbiological propertiesvis-à-
vis radioprotection. (2008). Indian Journal of Radiation Research, 5(3-4), 108-109.
25. Rajesh Arora, Atlar Singh Dhaker, Manish Adhikari et al. Evaluation of antioxidant and
radioprotective efficacy of a dietary ingredient commonly used in Indian Cuisine. (2008).
Indian Journal of Radiation Research, 5(3-4), 109.
PRESENTATIONS
1. Presented seminar on “Enhancement of Melanoma Sensitization by Cold Atmospheric
Plasmaassistedherbal based nanoemulsionmolecules:ABiological studies” presented at
2nd
InternationalProfessorsworkshoponPlasmaInformationExchange (IPWPIE2
2018), 80th
AnniversaryHall,KwangwoonUniversity,Seoul,Koreaon30th
November2018 (Invited Oral
Talk).
2. Presentation on “Combined effect of cold plasma and nanoemulsion based drug delivery
system for targetinghumanmelanoma” presentat8th
International SymposiumonPlasma
Biosciences (ISPB 2018), Songdo Convensia, Incheon, Korea from 24th
to 28th
July 2018
(Invited Oral Talk).
3. Topic entitled “Characterization and Biological studies of Self Nano Emulsifying Drug
Delivery System (SNEDDS) with cold atmosphericplasmaagainsthuman melanoma cells”
was presented at International Forum on Functional Material 2017 (IFFM-2017), Ramada
Plaza Jeju Hotel, Jeju, Korea from 27th
to 29th
June 2017 (Invited Oral Talk).
4. The work entitield“Silymarincan be used as a safe radiation countermeasure in the event
of radiological emergencies: Evidence from in vitro and in vivo studies.” was presented at
International Conference on Radiation Biology (ICRB-2012), ACTREC, Navi Mumbai, India
from 11th
to 13th
November-2012 (Poster presentation).
5. Presented work “The Radioprotective Properties of Silymarin for the Management of
Radiological Incidents” at International Conference on Radiation Biology (ICRB-2010), Sri
Ramachandra Medical College, Porur, Chennai, India from 28th
to 30th
November 2010
(Poster presentation).
6. The work entitled“UpregulationofEndogenousAntioxidant System by Dietary Ingredient
IBG15 Results in Mitigation of lonizing Radiation-Induced Conditioned Taste Aversion
(CTA) in Sprague Dawley Rats” has been presented at the International Conference on
Radiation Biology (ICRB-2010), Sri Ramachandra Medical College, Porur, Chennai, India
from 28th
to 30th
November 2010 (Poster presentation).
7. Presented work “Study on the aqueous extract of INM-2008 as a potent antioxidant and
putative radio protector” at International Conference on Radiation Biology (ICRB-2008),
Jaipur, India November 2010 (Poster presentation).
AWARDS AND MEMBERSHIPS
1. Postdoctoral Scientist at The George Washington University for in vivo studies using cold
atmospheric plasma technique, 22nd
April 2019 – till now.
2. Invitedas sessionchairof PlasmaCharacterization I at the Joint International Conference
on ICMAP 2018, APCPST 2018 and ISPB 2018 which was held at Songdo ConvensiA in
Incheon, South Korea during July 24-28, 2018.
3. Life-time membership of scholarly association by “Healthcare & Biological Science
Research Association” (HBSRA), approved by Eurasia Research, (Membership ID: HBSRA-
M18105).
8. 8
4. Certificate and training on laboratory animal use and management, conducted and
awarded by Korean association for laboratory animals (KAFLA) on 15th
September 2017 at
Daejeon, South Korea.
5. Post Doctoral Researcher at Plasma Bioscience Research Centre, Kwangwoon University,
Seoul,Republic of South Korea for carrying out research on therapeutic activity of Plasma
on Human Melanoma, 18th
September 2016 – 15th
April 2019.
6. Certificate and in-hand experience BD FACSOrient training course on BD LSR II flow
cytometer at BD-Jamia Hamdard FACS academy, 27th
June 2016-29th
June 2016.
7. European Union-Marie Curie Actions TRAVEL GRANT (Grant No.: EU FP7 Project PIRSES-
GA-2012-316067) for International Research Staff Exhange Scheme entitled “Studies on
some herbal additivesgivingpartial protectionagainsttoxicorimmunosuppressive effects
of some mycotoxins and improving wound granulation” organized at Department of
Medical Faculty, Trakia University, Stara Zagora, Bulgaria from 04th
December 2014 to 19
February 2015.
8. Best poster award- The work entitled “Evaluationofradioprotectiveefficacyofsilymarin”
has been adjudged as one of the best poster presentation at Medical Conference, Stara
Zagora, Bulgaria on 23rd
August-2012.
9. DRDO Research Associate fellowship for carrying out further research in the field of
radiation biology and biomedical sciences.
10. DRDO Junior & Senior Research Fellowship for carrying out doctoral research in the field
of biomedical sciences.
DECLARATION:
I hereby declare that all the above-mentioned facts are true to the best of my knowledge.
Place: Washington DC, USA (Manish Adhikari)
Name and Contact Address of Referees
Dr. Paban Agrawala
Scientist“E”,Divisionof
RadiationBiosciences,Institute
of NuclearMedicine &Allied
Sciences,BrigSKMazumdar
Marg, Delhi-110054,India
+91-9891479719
paban@inmas.drdo.in
Prof Michael Keidar
Professor
Mechanical and Aerospace
Engineering, The George
WashingtonUniversity
Science &EngineeringHall 3550
800 22nd Street,NW
Washington,DC20052, USA
+1-202-994-6929
keidar@gwu.edu
Prof Eun Ha Choi
Directorand Professor
PlasmaBioscience Research
Center,Kwangwoon
University,Dasanjae 102,
Nowon-Gu,Seoul 01897,
SouthKorea
+82-10-22405236
ehchoi@kw.ac.kr