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Exotic Cotton Genotypes
Fiber Quality
and Their Effects on
Jeevan Adhikari
jeevan@uga.edu
swicofil.comwikipedia.org cottoninc.com
Fiber Quality???
Craig Taylor
220 thread count
$525
Thomas Pink
170 thread count
$350
us.thomaspink.com craigtaylor.com
beliefnet.comwordpress.com
Fiber Quality???
telegraph.com
Introduction
Exotic Cotton Genotypes and Fiber Quality
The cotton world
Cotton breeding
Challenges and opportunities
Research
Objectives
Materials and methods
Results
Expected outcome
Summary
The Cotton world
Introduction
Cotton Breeding
Challenges and
Opportunities
zazzle.com art.com sheinside.com
Cotton
 Major source of natural fiber
 “White Gold” – soft fluffy
staple fibers
 Significant economic impact
worldwide
 4th largest crop in USA
cropsoilandmore.com
Types of Cotton
 New world cotton  Old world cotton
Tree cotton – G. arboreum
Levant cotton – G. herbaceum
Upland cotton – G. hirsutum
Pima cotton – G. barbadense
cotton.org
cotton.org
The Cotton World
World Leaders in Cotton
USAChina BrazilIndia AustraliaPakistan Uzbekistan
33
29
7.5
13
11
10
7.4
2.5
4.5
4 4.2 2.8
Production (Billion kg)
Export (Billion kg)
cotton.org
0.1
0.5
30
Cotton in United States
wordpress.com
72
125
30
30
Texas
Georgia
California
Arkansas
N. Carolina
usda.org
Production (Million kg)
The State of Georgia
800
0
4800
4000
3200
2400
1600
6400
5600
2006 2007 2008 2009 2010 2011
Area planted (Square km)
georgiaagforecast.com
Cotton
Peanut
Corn
SoybeanWheat
Georgia
EasternAcala DeltaPlains
US Cotton Genotypes
cottoninc.com
PD94042Acala Maxxa DES56PM HS 200
The cotton world
Introduction
Cotton breeding
Challenges and
opportunities
zazzle.com art.com sheinside.com
Yield
southwestfarmpress.com tumblr.com laurachenoweth.com
Other Traits
Insect-pest resistance
Boll worm Spider mitesStink bug Plant bug
Disease resistance
Boll rot Wilt NematodesLeaf spot
flickr.com sripmc.org clemson.edu fightbugs.com today.agrilife.org mississippi-crops.com
Fiber color
Fiber quality
Fiber length Fiber fineness Fiber strength
Other Traits
mavensphotoblog.comdoa.go.th cottonindia.com cirad.com environmentalleader.com
Fiber Length
 Long fibers make full and even yarns.
 Long fibers reduce hairiness in yarns.
cottonpromotion.com studyblue.comtextilefabric.com
 Longer fibers are desirable in cotton industry.
Fiber Fineness
 Fiber strength ∞ Fiber fineness
 Coarse fiber produces heavy textiles.
 Coarse fiber reduces thread count.
 Very fine fiber produces weak fabric.
 Thread count ∞ Fabric quality ∞ Price
elevenpark.comcozyclothes.com
Fiber length
Fineness
Strength
Short Medium Long Extra long
Discount Base Premium Base Discount
Very weak Weak Base Strong
US Cotton Cultivars
cottoninc.org
The cotton world
Introduction
Cotton breeding
Challenges and
opportunities
zazzle.com art.com sheinside.com
Challenges
Negative association between yield and fiber quality
Genetic complexity of the trait
Narrow genetic diversity of cultivated cotton species
Negative Association Between
Yield and Fiber Quality
2.65
2.85
3.05
3.25
3.45
3.65
Length(cm)
1,000 2,000
Kg/sq.km
260
280
300
320
360
340
380
400
1,000 2,000
lbs/acre
Yield and fiber length Yield and fiber strength
Strength(KNmKg-1)
US 1999-2009 US 1999-2009
Clement et al. 2012
Kg/sq.km
Complex Nature of the Traits
 Multigenic
 Small additive effects
 Genes interact
 Influenced by the environment
specialityserpents.comtulane.eduwordpress.comestrellamountain.com
Reduced Genetic Diversity
 Formation of polyploid “AD” cotton
A
DA
AD
abcteach.com
Reduced Genetic Diversity
 Migration and distribution
AD
abcteach.com
Reduced Genetic Diversity
 Domestication and selection
abcteach.com
Opportunities
Negative association between yield and fiber quality
Positive association between fiber
length and fiber strength
Clement et al., 2012
1,000 2,000 3,000
Kg/sq.Km
2.65
2.85
3.05
3.25
3.45
3.65
Length(cm)
Yield and fiber length
US
1999-2009
Australia
2004-2009
Opportunities
 Linkage can be broken
1,000 2,000 3,000
Kg/sq.Km
260
280
300
320
360
340
380
400
Strength(KNmKg-1)
Yield and fiber strength
US
1999-2009
Australia
2004-2009
Opportunities
 Linkage can be broken
Clement et al., 2012
Opportunities
Negative association between yield and fiber quality
Positive association between fiber
length and fiber strength
Genetic complexity of the trait
Novel tools and techniques are
available
Opportunities
 Genetic markers help to dissect complex traits
Next exit
Dwarf Gene
2 cM
 Whole genome sequence available
Gossypium raimondii Gossypium hirsutum
specialneeds.com cicr.com wordpress.com wikipedia.org
Opportunities
Negative association between yield and fiber quality
Positive association between fiber
length and fiber strength
Genetic complexity of the trait
Novel tools and techniques are
available
Narrow genetic diversity of cultivated cotton species
More than 50 different species (wild
and cultivated) in Gossypium genus
Opportunities
 Wide diversity in the wild
crops.com usda.org cottoninc.com
 Verticillium wilt resistant
Opportunities
 Salt tolerant
 Naturally defoliating
 High oil content
 High fiber quality parameters
(Abdurakhomonov et al., 2005)
(Abdurakhomonov et al., 2005)
(McCarty and Jenkins , 1998)
(Iqbal et al., 2001)
(Smith et al, 1995)
cottoninc.com
Introduction
Exotic Cotton Genotypes and Fiber Quality
The cotton world
Cotton breeding
Challenges and opportunities
Research
Objectives
Materials and methods
Results
Expected outcome
Summary
Research
Objectives Materials and methods
Results Expected outcome
Objectives
 Explore exotic cotton accessions to increase
the genetic base of cultivated cotton.
 Evaluate the effects of exotic genotypes on
fiber quality traits.
 Evaluate the effects of different genetic
backgrounds on fiber quality traits.
Research
Objectives Materials and methods
Results Expected outcome
Plant Materials
PD94042Acala Maxxa DES56PM HS 200
MDN063
MDN257
MDN101
Genotype Trait Ranking Additive genetic variance in crosses with DP16
Yield
Lint%
Bollsize
Micronaire
2.5%Span
length
Elongation
Strength
Height
MDN063 Micronaire 1 (of 79) 0 -- ++ ++ -- ++ -- 0
MDN101 Selection
Index
1 (of 79) ++ ++ 0 0 ++ 0 + ++
MDN257 Length 1 (of 79) 0 -- -- - ++ -- 0 0
Why these three genotypes?
McCarty and Jenkins, 1993
Experimental populations
MDN101
DES56
Acala Maxxa
PM HS 200
PD94042
MDN257
MDN063
Exotic Parent Elite Parent
F1 Progeny
F2 Progeny
F3 Progeny
DNA extraction
Phenotype scoring
Population Development
Fiber quality traits scored
wattention.com swicofil.com
Fiber length Fiber strengthFiber fineness
uster.com
Fiber uniformity index Fiber elongation Short fiber content %
www.cirad.fr www.independentstitch.typepad.com
Research
Objectives Materials and methods
Results Expected outcome
0
2
4
6
8
10
12
14
16
18
77
78
79
80
81
82
83
84
85
86
MDN 101 Maxxa
F2
Uniformity index (%)
Frequency
Frequency
Fiber Length (cm)
0
2
4
6
8
10
12
14
16
18
2.5
2.9
3.3
3.7
4.1
4.5
4.9
5.3
5.7
6.1
6.5
MDN 101Maxxa
F2
Frequency
Fiber Fineness(millitex)
Frequency
Fiber Elongation (%)
Results
0
5
10
15
20
25
2.26
2.36
2.46
2.57
2.67
2.77
2.87
2.97
3.07
3.18
3.28
Frequency
Maxxa MDN 101
0
5
10
15
20
25
30
3.3
3.5
3.8
4.0
4.3
4.5
4.8
5.0
5.3
5.5
5.8
6.0
6.3
6.5
Frequency
MaxxaMDN 101
Traits UHM UI STR ELO SFC
MIC F2 0.03 0.23 0.05 0.33 -0.41*
F3 0.08 0.32 0.28 0.18 -0.59**
UHM F2 0.59** 0.73** 0.27 -0.56**
F3 0.74** 0.74** -0.31 -0.4*
UI F2 0.63** 0.45* -0.74**
F3 0.67** -0.12 -0.62**
STR F2 0.62** -0.69**
F3 -0.37 -0.49*
ELO F2 -0.65**
F3 0.003
** Significant at P= 0.01 * Significant at P= 0.05
Correlation
Results
Ongoing and Future Works
 Genotyping of the populations
 QTLs and/or alleles identification
 Correlating genotypic and phenotypic data
 Preparation of genetic maps
 Advancing populations for further selection
Research
Objectives Materials and methods
Results Expected outcome
Expected Outcome
 Identification of QTLs governing fiber quality
traits.
 Mapping of the QTLs to the existing genetic
map of cotton.
Summary
 Exotic lines introduce traits of value to the
cultivated gene pool.
 Cotton is an important fiber crop.
 Cultivated cotton gene pool is poor in valuable
genes and/or alleles.
 Exotic genotypes are a good source of diverse
genes and/or alleles.
 Genetic mapping and locating the genes
governing these valuable traits help in breeding
superior cotton cultivars.
“Strength lies in differences, not in similarities”
- Stephen R. Covey
THANK YOU
Embrace Diversity

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CRSS_8100_MS_March-05-2014

  • 1. Exotic Cotton Genotypes Fiber Quality and Their Effects on Jeevan Adhikari jeevan@uga.edu swicofil.comwikipedia.org cottoninc.com
  • 2. Fiber Quality??? Craig Taylor 220 thread count $525 Thomas Pink 170 thread count $350 us.thomaspink.com craigtaylor.com
  • 4. Introduction Exotic Cotton Genotypes and Fiber Quality The cotton world Cotton breeding Challenges and opportunities Research Objectives Materials and methods Results Expected outcome Summary
  • 5. The Cotton world Introduction Cotton Breeding Challenges and Opportunities zazzle.com art.com sheinside.com
  • 6. Cotton  Major source of natural fiber  “White Gold” – soft fluffy staple fibers  Significant economic impact worldwide  4th largest crop in USA cropsoilandmore.com
  • 7. Types of Cotton  New world cotton  Old world cotton Tree cotton – G. arboreum Levant cotton – G. herbaceum Upland cotton – G. hirsutum Pima cotton – G. barbadense cotton.org
  • 9. World Leaders in Cotton USAChina BrazilIndia AustraliaPakistan Uzbekistan 33 29 7.5 13 11 10 7.4 2.5 4.5 4 4.2 2.8 Production (Billion kg) Export (Billion kg) cotton.org 0.1 0.5
  • 10. 30 Cotton in United States wordpress.com 72 125 30 30 Texas Georgia California Arkansas N. Carolina usda.org Production (Million kg)
  • 11. The State of Georgia 800 0 4800 4000 3200 2400 1600 6400 5600 2006 2007 2008 2009 2010 2011 Area planted (Square km) georgiaagforecast.com Cotton Peanut Corn SoybeanWheat Georgia
  • 12. EasternAcala DeltaPlains US Cotton Genotypes cottoninc.com PD94042Acala Maxxa DES56PM HS 200
  • 13. The cotton world Introduction Cotton breeding Challenges and opportunities zazzle.com art.com sheinside.com
  • 15. Other Traits Insect-pest resistance Boll worm Spider mitesStink bug Plant bug Disease resistance Boll rot Wilt NematodesLeaf spot flickr.com sripmc.org clemson.edu fightbugs.com today.agrilife.org mississippi-crops.com
  • 16. Fiber color Fiber quality Fiber length Fiber fineness Fiber strength Other Traits mavensphotoblog.comdoa.go.th cottonindia.com cirad.com environmentalleader.com
  • 17. Fiber Length  Long fibers make full and even yarns.  Long fibers reduce hairiness in yarns. cottonpromotion.com studyblue.comtextilefabric.com  Longer fibers are desirable in cotton industry.
  • 18. Fiber Fineness  Fiber strength ∞ Fiber fineness  Coarse fiber produces heavy textiles.  Coarse fiber reduces thread count.  Very fine fiber produces weak fabric.  Thread count ∞ Fabric quality ∞ Price elevenpark.comcozyclothes.com
  • 19. Fiber length Fineness Strength Short Medium Long Extra long Discount Base Premium Base Discount Very weak Weak Base Strong US Cotton Cultivars cottoninc.org
  • 20. The cotton world Introduction Cotton breeding Challenges and opportunities zazzle.com art.com sheinside.com
  • 21. Challenges Negative association between yield and fiber quality Genetic complexity of the trait Narrow genetic diversity of cultivated cotton species
  • 22. Negative Association Between Yield and Fiber Quality 2.65 2.85 3.05 3.25 3.45 3.65 Length(cm) 1,000 2,000 Kg/sq.km 260 280 300 320 360 340 380 400 1,000 2,000 lbs/acre Yield and fiber length Yield and fiber strength Strength(KNmKg-1) US 1999-2009 US 1999-2009 Clement et al. 2012 Kg/sq.km
  • 23. Complex Nature of the Traits  Multigenic  Small additive effects  Genes interact  Influenced by the environment specialityserpents.comtulane.eduwordpress.comestrellamountain.com
  • 24. Reduced Genetic Diversity  Formation of polyploid “AD” cotton A DA AD abcteach.com
  • 25. Reduced Genetic Diversity  Migration and distribution AD abcteach.com
  • 26. Reduced Genetic Diversity  Domestication and selection abcteach.com
  • 27. Opportunities Negative association between yield and fiber quality Positive association between fiber length and fiber strength
  • 28. Clement et al., 2012 1,000 2,000 3,000 Kg/sq.Km 2.65 2.85 3.05 3.25 3.45 3.65 Length(cm) Yield and fiber length US 1999-2009 Australia 2004-2009 Opportunities  Linkage can be broken
  • 29. 1,000 2,000 3,000 Kg/sq.Km 260 280 300 320 360 340 380 400 Strength(KNmKg-1) Yield and fiber strength US 1999-2009 Australia 2004-2009 Opportunities  Linkage can be broken Clement et al., 2012
  • 30. Opportunities Negative association between yield and fiber quality Positive association between fiber length and fiber strength Genetic complexity of the trait Novel tools and techniques are available
  • 31. Opportunities  Genetic markers help to dissect complex traits Next exit Dwarf Gene 2 cM  Whole genome sequence available Gossypium raimondii Gossypium hirsutum specialneeds.com cicr.com wordpress.com wikipedia.org
  • 32. Opportunities Negative association between yield and fiber quality Positive association between fiber length and fiber strength Genetic complexity of the trait Novel tools and techniques are available Narrow genetic diversity of cultivated cotton species More than 50 different species (wild and cultivated) in Gossypium genus
  • 33. Opportunities  Wide diversity in the wild crops.com usda.org cottoninc.com
  • 34.  Verticillium wilt resistant Opportunities  Salt tolerant  Naturally defoliating  High oil content  High fiber quality parameters (Abdurakhomonov et al., 2005) (Abdurakhomonov et al., 2005) (McCarty and Jenkins , 1998) (Iqbal et al., 2001) (Smith et al, 1995) cottoninc.com
  • 35. Introduction Exotic Cotton Genotypes and Fiber Quality The cotton world Cotton breeding Challenges and opportunities Research Objectives Materials and methods Results Expected outcome Summary
  • 36. Research Objectives Materials and methods Results Expected outcome
  • 37. Objectives  Explore exotic cotton accessions to increase the genetic base of cultivated cotton.  Evaluate the effects of exotic genotypes on fiber quality traits.  Evaluate the effects of different genetic backgrounds on fiber quality traits.
  • 38. Research Objectives Materials and methods Results Expected outcome
  • 39. Plant Materials PD94042Acala Maxxa DES56PM HS 200 MDN063 MDN257 MDN101
  • 40. Genotype Trait Ranking Additive genetic variance in crosses with DP16 Yield Lint% Bollsize Micronaire 2.5%Span length Elongation Strength Height MDN063 Micronaire 1 (of 79) 0 -- ++ ++ -- ++ -- 0 MDN101 Selection Index 1 (of 79) ++ ++ 0 0 ++ 0 + ++ MDN257 Length 1 (of 79) 0 -- -- - ++ -- 0 0 Why these three genotypes? McCarty and Jenkins, 1993
  • 42. Exotic Parent Elite Parent F1 Progeny F2 Progeny F3 Progeny DNA extraction Phenotype scoring Population Development
  • 43. Fiber quality traits scored wattention.com swicofil.com Fiber length Fiber strengthFiber fineness uster.com Fiber uniformity index Fiber elongation Short fiber content % www.cirad.fr www.independentstitch.typepad.com
  • 44. Research Objectives Materials and methods Results Expected outcome
  • 45. 0 2 4 6 8 10 12 14 16 18 77 78 79 80 81 82 83 84 85 86 MDN 101 Maxxa F2 Uniformity index (%) Frequency Frequency Fiber Length (cm) 0 2 4 6 8 10 12 14 16 18 2.5 2.9 3.3 3.7 4.1 4.5 4.9 5.3 5.7 6.1 6.5 MDN 101Maxxa F2 Frequency Fiber Fineness(millitex) Frequency Fiber Elongation (%) Results 0 5 10 15 20 25 2.26 2.36 2.46 2.57 2.67 2.77 2.87 2.97 3.07 3.18 3.28 Frequency Maxxa MDN 101 0 5 10 15 20 25 30 3.3 3.5 3.8 4.0 4.3 4.5 4.8 5.0 5.3 5.5 5.8 6.0 6.3 6.5 Frequency MaxxaMDN 101
  • 46. Traits UHM UI STR ELO SFC MIC F2 0.03 0.23 0.05 0.33 -0.41* F3 0.08 0.32 0.28 0.18 -0.59** UHM F2 0.59** 0.73** 0.27 -0.56** F3 0.74** 0.74** -0.31 -0.4* UI F2 0.63** 0.45* -0.74** F3 0.67** -0.12 -0.62** STR F2 0.62** -0.69** F3 -0.37 -0.49* ELO F2 -0.65** F3 0.003 ** Significant at P= 0.01 * Significant at P= 0.05 Correlation Results
  • 47. Ongoing and Future Works  Genotyping of the populations  QTLs and/or alleles identification  Correlating genotypic and phenotypic data  Preparation of genetic maps  Advancing populations for further selection
  • 48. Research Objectives Materials and methods Results Expected outcome
  • 49. Expected Outcome  Identification of QTLs governing fiber quality traits.  Mapping of the QTLs to the existing genetic map of cotton.
  • 50. Summary  Exotic lines introduce traits of value to the cultivated gene pool.  Cotton is an important fiber crop.  Cultivated cotton gene pool is poor in valuable genes and/or alleles.  Exotic genotypes are a good source of diverse genes and/or alleles.  Genetic mapping and locating the genes governing these valuable traits help in breeding superior cotton cultivars.
  • 51. “Strength lies in differences, not in similarities” - Stephen R. Covey THANK YOU Embrace Diversity