Column chromatography is a technique used to separate components of a mixture using a glass column packed with a stationary phase. The mixture is dissolved in a mobile phase which flows through the column, separating the components based on their interactions with the stationary phase. Key factors that affect column chromatography include the stationary phase material, column dimensions, mobile phase used, and temperature. Column chromatography has applications in purifying compounds, isolating drug constituents, and separating mixtures.
COLUMN CHROMATOGRAPHY - SEPERATION OF THE MIXTURE OF COMPONENTS IJN TO INDIVIDUAL COMPONENTS BY USING STATIONARY PHASE AND MOBILE PHASE UPON THE USING OF COLUMN
Thin-layer chromatography (TLC) is a chromatography technique used to separate non-volatile mixtures. Thin-layer chromatography is performed on a sheet of glass, plastic, or aluminium foil, which is coated with a thin layer of adsorbent material, usually silica gel, aluminium oxide (alumina), or cellulose.
COLUMN CHROMATOGRAPHY - SEPERATION OF THE MIXTURE OF COMPONENTS IJN TO INDIVIDUAL COMPONENTS BY USING STATIONARY PHASE AND MOBILE PHASE UPON THE USING OF COLUMN
Thin-layer chromatography (TLC) is a chromatography technique used to separate non-volatile mixtures. Thin-layer chromatography is performed on a sheet of glass, plastic, or aluminium foil, which is coated with a thin layer of adsorbent material, usually silica gel, aluminium oxide (alumina), or cellulose.
Chromatography is a powerful and advanced techniques for separating mixtures. Many types of chromatographic techniques are known, such as paper, thin layer, column chromatography, each with its own strength and weakness.
Chromatography system in general have a stationary phase and a mobile phase.
In column chromatography both phases are placed in a column container, i.e. all the chromatographic operations are carried out using column.
Column chromatography in chemistry is a method using for the Identification, separation and purify individual chemical compounds from mixtures of compounds in the large amount.
Column Chromatography is a separation technique in which components of mixture is separated by using a glass column packed with stationary phase and liquid mobile phase flowing continuously through the column.
It is suitable for the physical separation of gram quantities of material. A solvent acts as the mobile phase while a finely divided solid surface acts as the stationary phase.
Usually a glass tube with a diameter from 1cm to 10cm and a height of 20 cm to 50cm with a tap at the bottom, is used for this purpose.
Depending upon the flow of solvent down, column chromatography may be separated into two categories.
Gravity column chromatography
If the solvent is allowed to flow down the column by Gravity, or downward process, it is known as gravity column chromatography .
Flash chromatography
If the solvent is forced down the column by positive air pressure , it is called flash chromatography.
Objectives, applications & Theories in FILTRATIONAkankshaPatel55
Filtration is a physical separation process that separates solid matter and fluid from a mixture using a filter medium that has a complex structure through which only the fluid can pass. Solid particles that cannot pass through the filter medium are described as oversize and the fluid that passes through is called the filtrate. Oversize particles may form a filter cake on top of the filter and may also block the filter lattice, preventing the fluid phase from crossing the filter, known as blinding. The size of the largest particles that can successfully pass through a filter is called the effective pore size of that filter.
Filtration is used in many different applications, including:
Water treatment: to remove impurities from water, such as sediment, bacteria, and viruses.
Air filtration: to remove dust, pollen, and other allergens from air.
Oil filtration: to remove impurities from oil, such as dirt, metal shavings, and water.
Chemical processing: to separate solids from liquids in chemical reactions.
Food and beverage processing: to clarify liquids, such as juice, wine, and beer.
There are many different types of filters, each with its own advantages and disadvantages. Some common types of filters include:
Depth filters: These filters are made of a thick layer of material, such as paper, cloth, or sand. The solid particles are trapped in the pores of the filter medium.
Surface filters: These filters are made of a thin layer of material, such as a membrane. The solid particles are trapped on the surface of the filter medium.
Cartridge filters: These filters are self-contained units that can be easily replaced.
Bag filters: These filters are made of a bag of fabric that is suspended in a housing. The solid particles are trapped in the fabric of the bag.
Chromatography is a powerful and advanced techniques for separating mixtures. Many types of chromatographic techniques are known, such as paper, thin layer, column chromatography, each with its own strength and weakness.
Chromatography system in general have a stationary phase and a mobile phase.
In column chromatography both phases are placed in a column container, i.e. all the chromatographic operations are carried out using column.
Column chromatography in chemistry is a method using for the Identification, separation and purify individual chemical compounds from mixtures of compounds in the large amount.
Column Chromatography is a separation technique in which components of mixture is separated by using a glass column packed with stationary phase and liquid mobile phase flowing continuously through the column.
It is suitable for the physical separation of gram quantities of material. A solvent acts as the mobile phase while a finely divided solid surface acts as the stationary phase.
Usually a glass tube with a diameter from 1cm to 10cm and a height of 20 cm to 50cm with a tap at the bottom, is used for this purpose.
Depending upon the flow of solvent down, column chromatography may be separated into two categories.
Gravity column chromatography
If the solvent is allowed to flow down the column by Gravity, or downward process, it is known as gravity column chromatography .
Flash chromatography
If the solvent is forced down the column by positive air pressure , it is called flash chromatography.
Objectives, applications & Theories in FILTRATIONAkankshaPatel55
Filtration is a physical separation process that separates solid matter and fluid from a mixture using a filter medium that has a complex structure through which only the fluid can pass. Solid particles that cannot pass through the filter medium are described as oversize and the fluid that passes through is called the filtrate. Oversize particles may form a filter cake on top of the filter and may also block the filter lattice, preventing the fluid phase from crossing the filter, known as blinding. The size of the largest particles that can successfully pass through a filter is called the effective pore size of that filter.
Filtration is used in many different applications, including:
Water treatment: to remove impurities from water, such as sediment, bacteria, and viruses.
Air filtration: to remove dust, pollen, and other allergens from air.
Oil filtration: to remove impurities from oil, such as dirt, metal shavings, and water.
Chemical processing: to separate solids from liquids in chemical reactions.
Food and beverage processing: to clarify liquids, such as juice, wine, and beer.
There are many different types of filters, each with its own advantages and disadvantages. Some common types of filters include:
Depth filters: These filters are made of a thick layer of material, such as paper, cloth, or sand. The solid particles are trapped in the pores of the filter medium.
Surface filters: These filters are made of a thin layer of material, such as a membrane. The solid particles are trapped on the surface of the filter medium.
Cartridge filters: These filters are self-contained units that can be easily replaced.
Bag filters: These filters are made of a bag of fabric that is suspended in a housing. The solid particles are trapped in the fabric of the bag.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...University of Maribor
Slides from:
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Track: Artificial Intelligence
https://www.etran.rs/2024/en/home-english/
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
2. CONTENT
▶ History and introduction to chromatography
▶ Principle of column chromatography
▶ Requirements for column chromatography
▶ Factors affecting column chromatography
▶ Applications
3. History & introduction to
chromatography
▶ M. Tswett (1906) defined chromatography as the method
in which the components of a mixture are separated on an
adsorbent column in a flowing system.
▶ The international union of pure & applied
chemistry(IUPAC) definition of chromatography:
▶ “Chromatography is a physical method of separation in
which the components to be separated are distributed
between two phases, one of which is stationary, while the
other(mobile phase)moves in a definite direction”
4. Column chromatography
▶ Introduction:
▶ Column chromatography is a separation technique in
which components of mixture is separated by using a glass
column packed with stationary phase and the liquid
mobile phase flowing continuously through the column.
▶ Principle:
▶ Column adsorption chromatography
5. Principle
▶ A solid stationary phase and a liquid mobile phase is used and the
principle of separation is adsorption.
▶ When a mixture of component dissolved in the mobile phase, & is
introduced in to the column, the individual component move with
different rate depending upon the relative affinities.
▶ The compound with less affinity toward the stationary phase
(adsorbent) moves faster and hence eluted out of the column first.
The one with greater affinity towards the stationary phase moves
slower down the column & hence it is eluted later. Thus the
compounds are separated.
▶ The type of interaction between the stationary phase and the solute is
reversible in nature.
▶ The rate of movement of a component(R) is given by:
▶ R = rate of movement of component rate of movement of mobile
phase
6. Practical requirements
▶ 1. Column characteristics & selection
▶ 2. Stationary phases
▶ 3. Mobile phases
▶ 4. Preparation of column
▶ 5. Introduction of sample
▶ 6. Development of column
▶ 7. Detection of components
▶ 8. Recovery of components
7. COLUMN CHARACTERISTICS
▶ The material of the column is mostly good quality neutral glass since it should not
be affected by solvent, acid or alkali.
▶ The column dimension are important for effective column separations.
▶ The length : diameter ratio ranges from 10:1 to 30:1, for more efficiency the
length : diameter ratio ranges from 100:1
▶ The length of the column depends upon:
▶ Number of compounds to be separated
▶ Type of adsorbent used
▶ Quantity of the sample
▶ Affinity of compounds towards the adsorbent used
▶ Better separation will be obtained with a long narrow column than short thick
column because number of theoretical plates will be more.
10. MOBILE PHASE OR ELUENTS OR SOLVENTS
USED
▶ Mobile phase act as a solvent to introduce the mixture into the column as
developer to develop the zones for separation and as an eluent to remove the
pure component out to the column.
▶ Strain(1942) has arranged the solvents in order of eluting power. A grouping
of solvents in order of polarity index is known as eluotrophic series.
▶ Increasing eluting power: Light petroleum ether (petroleum ether, hexane,
heptane), Carbon tetra chloride, Cyclohexane, Carbon disulphide ,Benzene
Toluene, Chloroform, Ether Ethyl acetate, Acetone, Alcohols ,Water, Pyridine
Organic acids, Inorganic acids & bases.
11. Preparation (packing) of column:
▶ The bottom portion of the column is packed with cotton wool or glass
wool, above which the column of adsorbent is packed.
▶ After packing the column with the adsorbent, a similar paper dics is
kept on the top, so that the adsorbent layer is not disturbed, during
the introduction of the sample or mobile phase.
▶ There are two types of packing techniques:
▶ 1. Dry packing technique
▶ 2. wet packing technique
12. Dry packing technique
▶ In this technique, the required quantity of adsorbent is
packed in the column in dry form and the solvent allowed
to flow through the column till equilibrium is reached.
▶ Disadvantage:
▶ 1. air bubbles are entrapped in the column
▶ 2. cracks appear in the adsorbent present in the column
▶ 3. hence the uniformity flow character & clear band of
the separated component may not be obtained.
13.
14.
15. Wet packing technique:
▶ This is the ideal technique. The required amount of adsorbent is
mixed with the mobile phase in a beaker and poured in to the column.
▶ The stationary phase settles uniformly in the column.
▶ Advantages:
▶ 1. there is no entrapment of air bubbles.
▶ 2. there will not be any crack in the column of the adsorbent.
▶ 3. the band eluted from the column will be uniform and ideal for
separation.
16. Introduction of sample
▶ The sample which is usually a mixture of component is
dissolved in minimum quantity of the mobile phase.
▶ The entire sample is introduced into the column at once
and get adsorbed on the top portion of the column.
▶ From this zone, the individual samples can be separated
by a process of elution.
17. Development technique (elution)
▶ Elution:
▶ It is a common method used in column chromatography.
In this method a small volume of mixture to be separated
is added on the top of column & mobile phase is allowed
to flow through the column.
▶ There are two elution technique:
▶ 1. Isocratic elution technique
▶ 2. Gradient elution technique
18.
19. Isocratic & Gradient elution technique:
▶ Isocratic elution technique :
▶ In this elution technique, the same solvent composition or solvent of same
polarity is used through out the process of separation.
▶ Ex. Chloroform only
▶ Gradient elution technique :
▶ In this elution technique, solvents of gradually increasing polarity or
increasing elution strength are used during the process of separation.
▶ Initially low polar solvent is used followed by gradually increasing the polarity
of the solvent.
▶ Ex. Initially benzene, then chloroform, then Ethyl acetate etc.
20. Detection of component
▶ If the compounds separated in a column chromatography procedure are
colored, the progress of the separation can simply be monitored visually.
▶ If the compounds to be isolated from column chromatography are colorless.
In this case, different techniques are used;
▶ Using UV/visible detector.
▶ Using fluoresence detector.
▶ Refractive index detector.
▶ By monitoring the fraction by thin layer chromatography.
21. Recovery of components:
▶ The best technique to recover the components by process of elution.
▶ The components are called as eluate, the solvent are called as eleunt.
▶ Recovery is done by collecting different fraction of mobile phase of equal
volume like 10ml, 20ml, with fraction of 10 or 20 min.
▶ Eluting the sample: Components a, b, and c separate as column progresses,
Fractions can be collected in test tubes, vials, beakers.
▶ Analyze the fractions by thin-layer chromatography or by detectors.
22. Factor affecting column efficiency:
▶ 1. Dimension of the column: column efficiency has been improved by
increasing length/width ratio of the column.
▶ 2. Particle size of column packing: separation to be improved by decreasing
the particle size of the adsorbent.
▶ 3. Activity of the adsorbent
▶ 4. Temperature of the column: The speed of the elution increases at higher
temperatures.
▶ 5. Packing of the column: Dry or wet packing
▶ 6. Quality of solvents: solvents having low viscosities is giving better results.
23. Application of column chromatography
▶ ►Separation of mixture of compounds
▶ ►Purification process
▶ ►Isolation of active constituents
▶ ►Estimation of drugs in formulation
▶ ►Determination of primary and secondary glycosides in
digitalis leaf.
▶ ► separation of diastereomers & separation of
geometrical isomers.
24. Advantages & disadvantages:
▶ Advantages of C.C:
▶ Any type of mixture can be separated & Any quantity of mixture can be
separated
▶ Used as qualitative as well as quantitative analysis
▶ Wider choice of Mobile Phase
▶ Automation is possible
▶ Disadvantages of C.C:
▶ Time consuming
▶ more amount of Mobile Phase are required
▶ To over come this, HPLC is developed with a pump.