Fungi are non-green, plantlike multicellular organisms belonging to the kingdom Fungi. They grow on dead organic matter in damp and warm places. Moulds, Yeasts, mushrooms are common examples of fungi. (STRUCTURE OF FUNGI) Moulds can be easily observed as they grow on food, bread, fruits, leather and even on barks of the tree. Mushrooms can be seen growing during rainy seasons on damp logs, walls, on the ground.
Mushrooms - intro classification and productionDIVYA BHARDWAJ
identification of mushrooms
structure of mushrooms
human use
fungi culture
techniques used for mushroom culture
steps in culturing
composting
spawning
casing
pinning
harvesting
cultivation of paddy straw mushroom
Introduction to microbiology: Microbiology in daily life, Characteristics, and morphology of bacteria, fungi, virus, protozoa and algae. Control of micro-organisms- Growth curve; Influence of environmental factors on growth- PH, Water activity, O2 availability, Temperature, Pressure and Radiation.
Mushrooms - intro classification and productionDIVYA BHARDWAJ
identification of mushrooms
structure of mushrooms
human use
fungi culture
techniques used for mushroom culture
steps in culturing
composting
spawning
casing
pinning
harvesting
cultivation of paddy straw mushroom
Introduction to microbiology: Microbiology in daily life, Characteristics, and morphology of bacteria, fungi, virus, protozoa and algae. Control of micro-organisms- Growth curve; Influence of environmental factors on growth- PH, Water activity, O2 availability, Temperature, Pressure and Radiation.
Fungi is most abundantly found organism in earth, almost all parts of earth we found earth, here we represent some characteristic with their uses and disadvantages .
Fungi are eukaryotic organisms that include microorganisms such as yeasts, moulds and mushrooms. These organisms are classified under kingdom fungi.
PHARMACEUTICAL MICROBIOLOGY (BP303T) Unit-III Part-1 Study of morphology, cla...Ms. Pooja Bhandare
PHARMACEUTICAL MICROBIOLOGY (BP303T)Unit-IIIPart-1Study of morphology, classification, reproduction/replication and cultivation of fungi, Introduction fungi. Morphological Characteristics of fungi, CLASSIFICATION: Depending on cell morphology, fungi can be divided into 4 classes:
Moulds Yeasts ,Yeast like fungi and
Dimorphic fungi
Depending on their sexual spores formation fungi are divided into 4 classes:
Zygomycetes Ascomycetes
Basidiomycetes Dueteromycetes
Reproduction and sporulation;Vegetative, Asexual
and Sexual
Vegetative reproduction: Fragmentation ,Fission, budding, Sclerotia Rhizomorphs
Asexual reproduction: Zoospores
Sporangiospore, Conidia
Oidia Uredospores ,Basidiospores
Sexual reproduction:Planogametic copulation: Isogamy Heterogamy
Gametangial contact
Gametangial copulation Spermatization Somatogamy CULTIVATION OF FUNGI: Brain Heart Infusion (BHT) agar
Czapek’s agar
Mycobiotic agar Inhibitory mold agar (IMA)
Potato dextrose agar
Sabouraud’s dextrose agar (SDA):
Sabouraud’s heart infusion (SABHI) agar
Potato Flake agar
Potato dextrose-yeast extract agar (PDYA)
. Cornmeal agar
Malt extract agar (MEA)
This upload includes description of structure of microsporangium, microsporogenesis, pollen grain and megasporogenesis.
It will be helpful to the students for their quick reference.
The stem bears leaves, buds, flowers & fruits & helps to spread them out so that they can function properly. For example, it helps to spread out the leaves in such a way that they get the maximum possible sunlight and air..
It is a type of cyclic flow of nutrients between non- living components like soil, rock air and water and living organisms and micro oganism (Pseudomonas and Aeruginosa). It makes the biosphere dynamic. In oxygen cycle there is a cyclic flow of atmospheric oxygen. Photosynthesis releases oxygen in the atmosphere. Respiration absorbs oxygen. By photosynthesis plant produces organic molecules (C6 H12 O6). By respiration that organic molecule is oxidized to release energy and carbon oxide.
Fungi is most abundantly found organism in earth, almost all parts of earth we found earth, here we represent some characteristic with their uses and disadvantages .
Fungi are eukaryotic organisms that include microorganisms such as yeasts, moulds and mushrooms. These organisms are classified under kingdom fungi.
PHARMACEUTICAL MICROBIOLOGY (BP303T) Unit-III Part-1 Study of morphology, cla...Ms. Pooja Bhandare
PHARMACEUTICAL MICROBIOLOGY (BP303T)Unit-IIIPart-1Study of morphology, classification, reproduction/replication and cultivation of fungi, Introduction fungi. Morphological Characteristics of fungi, CLASSIFICATION: Depending on cell morphology, fungi can be divided into 4 classes:
Moulds Yeasts ,Yeast like fungi and
Dimorphic fungi
Depending on their sexual spores formation fungi are divided into 4 classes:
Zygomycetes Ascomycetes
Basidiomycetes Dueteromycetes
Reproduction and sporulation;Vegetative, Asexual
and Sexual
Vegetative reproduction: Fragmentation ,Fission, budding, Sclerotia Rhizomorphs
Asexual reproduction: Zoospores
Sporangiospore, Conidia
Oidia Uredospores ,Basidiospores
Sexual reproduction:Planogametic copulation: Isogamy Heterogamy
Gametangial contact
Gametangial copulation Spermatization Somatogamy CULTIVATION OF FUNGI: Brain Heart Infusion (BHT) agar
Czapek’s agar
Mycobiotic agar Inhibitory mold agar (IMA)
Potato dextrose agar
Sabouraud’s dextrose agar (SDA):
Sabouraud’s heart infusion (SABHI) agar
Potato Flake agar
Potato dextrose-yeast extract agar (PDYA)
. Cornmeal agar
Malt extract agar (MEA)
This upload includes description of structure of microsporangium, microsporogenesis, pollen grain and megasporogenesis.
It will be helpful to the students for their quick reference.
The stem bears leaves, buds, flowers & fruits & helps to spread them out so that they can function properly. For example, it helps to spread out the leaves in such a way that they get the maximum possible sunlight and air..
It is a type of cyclic flow of nutrients between non- living components like soil, rock air and water and living organisms and micro oganism (Pseudomonas and Aeruginosa). It makes the biosphere dynamic. In oxygen cycle there is a cyclic flow of atmospheric oxygen. Photosynthesis releases oxygen in the atmosphere. Respiration absorbs oxygen. By photosynthesis plant produces organic molecules (C6 H12 O6). By respiration that organic molecule is oxidized to release energy and carbon oxide.
Intestine part of the alimentary canal is prone to many infections which we term as nutritional diseases which may lead to its inflammation. The various infectious agents causing nutritional disorders are bacteria, virus, tapeworms, roundworms, threadworms, hookworm, pin worm etc. Here are some common nutritional diseases or disorders of the digestive system (marasmus)
INTRODUCTION OF ALTERNATIVE SOURCES OF ENERGY: We know that by the time conventional sources of energy will deplete soon due to improved technological progress and increased demand. To cater the rising demand, keeping a balance with limited with limited availability of conventional sources is very important. It is mandatory to look up to the alternative sources of energy which we also term as non-conventional sources of energy.
We perform many activities consciously or unconsciously, for example, when we eat food, our eyes help to locate the food, the nose smells it, hands bring food to the mouth, teeth chew and masticate it, the tongue pushes the food inside the alimentary canal, and so on. (NERVOUS SYSTEM FUNCTION) All these activities occur in a coordinate manner. The organ system in our body that brings about coordination and integration of body activities, is the nervous system.
LIKE BEGETS LIKE, which means young one resemble their parents, (MONOHYBRID CROSS & DIHYBRID CROSS) is the well-known dogma associated with heredity. Each species has similarities among themselves due to the cause of heredity. W.Bateson was the first one to coin the term genetics in 1905. It is derived from the greek word “genesis” means to grow into or to become . in other word, genetics is the study of heredity and variation.
Flowers are the reproductive parts of plants,which are responsible for the production of gametes or sex cells ((non flowering plants).
A flower is modified shoot in which the leaves are modified into floral parts. A blossom propagates a branch from a bud in the axil of a little leaf-like structure called the bract. A bract is commonly green due to presence of chlorophyll and is a very small structure (non flowering plants).
Events of fertilization
A] Pollination brings female and male gametophyte together- The male and female gametophytes must meet and unite their gametes to fertilize egg. This is done by pollination, in which pollen is placed on the stigma of the carpel.
B] Germination of pollen grain under suitable condition- The cytoplasm of the pollen grain absorbs sugar and water from the stigma and bulges out to produce a tube known as pollen tube by braking down exine of pollen grains. This germinating pollen tube grows down through the stigma and style of flower towards the micropyle of the ovary. This tube produces chemical, calcium such as that dissolves the tissues of the style and the tip of the pollen tube enters the ovary through the micropylar end occurring double fertilization.
The eyeball is a spherical structure having 2.5 diameters. Structurally, the eyeball has three layers:- sclerotic layer (outer layer); choroid (middle layer) and retina (inner layer).
Outermost layer contains sclera and cornea
Middle layer contains choroid, ciliary body and iris.
Innermost layer consists of retina
Human beings have always been perplexed about the origin and evolution of life on earth. For centuries, a number of theories and hypothesis have been suggested to explain the evolutionary process.
In the previous year, in a very short time life was created by some supernatural power. Most of the religions and civilizations still believe in it, however, they do not have a scientific explanation and more a matter of faith. There has always been an attempt by the scientists and naturalists with keen observation, to explain the evolution scientifically and to look for its evidences.
The process of evolution involves a gradual change or variation in the organisms generation after generation. It means that the organisms of present-day have arisen from ancestors that were simpler in an organisation.
It is a group of viruses that causes respiratory problems in animals and birds. Some viruses are not so dangerous, but due to biological reasons, some family members become lethal causing even death e.g. SARS (Severe acute respiratory syndrome)
MERS (Middle-east respiratory syndrome)
If we see at the sky for some time at night, then we will observe some stars forming groups with shapes. So, the shape formed by the group of stars is called a constellation diagram. The shapes of constellations resemble objects familiar to those people. Each constellation is signified by an animal, a human being, or some other objects it appears to resemble. All the constellations seem to move in the sky from east to west. This is due to the rotation of the earth on its axis from west to east.
The disease is the malfunctioning of the body organs due to one reason or the other. In other words, it is a disorder of the body. The state of the body when any of its normal functions are disturbed or when the structures are altered is called disease. We understand that to remain healthy, we need to do much more than just keep away from disease. CONGENITAL DISEASE: This type of disease is present right from the birth. This is caused either due to genetic disorders or gene mutation or environmental factors. These diseases are passed on from one generation to next generation. Some examples of congenital diseases are Haemophilia, Sickle cell anaemia, Colour blindness, Thalassaemia etc.
All living organisms are made up of a variety of body parts like roots, leaves, flowers, fruits, wood etc. in plants and bones, flesh, nerves etc in animals. These parts look different from each other, yet they are all composed of tiny units called cells. Moreover, many living organisms like Amoeba, Paramecium, Chlamydomonas etc. consist of only one cell.
All living beings are made up of cells. The structural and functional unit of life is a cell which is the building block of the body. New cell arises from the pre-existing cells by the process of cell division.
Cell division occurs in all living organisms. In unicellular organisms, cell division directly produces two individuals. In multicellular organisms or higher-level organisms, life begins from a single cell, as a zygote, whIch divides and redivides mitotically into a number of cells to form a complete organism.
In multicellular organisms, there are two types of cells.
a)The somatic cells or the body cells- They form the body of an organism.
b)The reproductive cells or sex cells- They are gamete-producing cells.
Bacteria are the simplest ,most primitive and unicellular organisms without a true nucleus. That is why they are prokaryotic. they are placed under kingdom Monera. they are found everywhere in air,water,soil,food,inside our body etc. they lack nucleus and other cell organelles of complex cellslike plants,they possess a cell wall. each bacterial cell has its nuclear material in the form of a single chromosome which is not enclosed in a nuclear membrane.
Atoms are too small to see even with a powerful microscope and too light to be weighed even on the most sensitive balance. The history of the discovery of the structure of an atom is fascinating but a complicated subject. Only 100 years ago, scientists believed that atoms were solid, indestructible particles. Since then many great scientists had contributed brilliantly to give us the today’s model of an atom. Over the centuries, many philosophers and scientists tried to develop a model of the atom.
Conduction of water in plants is less elaborate than that of animal as well as plants. Plants are less active, so their cells do not need quick supply of materials. So, the only substances which are to be supplied to a plant through vascular bundle are water and minerals, this process is called ascent of sap. These materials are not available from air. Transport system also helps to carry food prepared in the leaves to the various parts of the plant like stems , roots etc. Ascent of sap is executed by two types of vascular bundles: Xylem which transports water and Phloem which transports prepared food.
On the basis of the function and occurrence ,tissues present in animal body is called animal tissue. These animal tissue is classified into the following four types:
Epithelial tissue
Connective tissue
Muscular tissue
Nervous tissue
The ability of any living organism terms as acclimatization. It develops certain features which improves the chances of its survival in the changing environment. Plants and animals can adopt themselves in such a way that they can easily survive in the conditions where they use to live. We term this phenomenon adaptation also. Acclimatization is a trait of an organism. It favors the living organism by natural selection.
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1. CLASSIFICATION OF FUNGI: EASY
DRAWING OF ITS & ACTIVITIES
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CLASSIFICATION OF FUNGI
INTRODUCTION: –
Fungi are non-green, plantlike multicellular organisms belonging to the kingdom Fungi. They grow on dead
organic matter in damp and warm places. Moulds, Yeasts, mushrooms are common examples of fungi.
(STRUCTURE OF FUNGI) Moulds can be easily observed as they grow on food, bread, fruits, leather and
even on barks of the tree. Mushrooms can be seen growing during rainy seasons on damp logs, walls, on
the ground.
CHARACTERISTICS OF FUNGI: –
( tiny filaments of rhizopus that penetrate the substratum are called)
Fig#1 diagram of branching hyphae forming mycelium
* Fungi are non- green eukaryotes. ( cottony mass of fungus is called)
* It contains well membrane bound organelles like nucleus, mitochondria etc.
*It has a definite cell wall made up of fungal cellulose and chitin.
*Fungus thallus consists of tubular, branched and thread like structures called hyphae.
* The mass of hyphae forms a network which is called mycelium.
* The hyphae may be septate or aseptate.
* They do not contain chlorophyll, so they are heterotrophic.
* They cannot move freely hence non- motile.
* They reproduce mostly by spore formation (which is known as asexual reproduction) .
* Sexual reproduction may also take place sometime.
2. DIFFERENT TYPES OF FUNGI: – ( cottony mass of fungus is
called)
A) MOULDS: It is a type of fungi grown on dead organic matter like decaying fruits, old and destroyed
leather goods, and other perishable products ; that is why moulds are saprophytic in nature. They make their
appearance in the form of a fine, fluffy and cottony mass. Rhizopus and Mucor are the two common bread
moulds.
Fig#2 diagram of Rhizopus and Mucor
GENERAL STRUCTURE: – (STRUCTURE OF FUNGI) The cottony mass of both Rhizopus and Mucor
consists of mycelium which is made up of branched hyphae. Hyphae are tiny filaments containing cytoplasm
and nuclei. Their cell walls contain chitin. Living and growing part of fungi are hyphae. The mass of tangled
hyphae is known as Mycelium. The hyphae is aseptate i.e. the cytoplasm is not partitioned by septa into
separate cells. So the hyphae are multinucleate or coenocytic. These are of three types.
A) STOLONS: These run parallel to the surface and are slightly arched.
B) RHIZOIDAL HYPHAE: These are small and root-like hyphae produced in clusters from stolons at regular
intervals.
C) SPORANGIOPHORES: These are unbranched erect hyphae that arise in groups opposite to rhizoidal
hyphae. These bear sporangia which produce sporangiospores. ( simple mucor diagram)
In Mucor stolons are absent and rhizoids arise anywhere on the mycelium. In Rhizopus, many
sporangiophores arise from a single node on stolonr, while in Mucor only one sporangiophore arises from a
single node. ( hyphae that grow into a cottony mass)
NUTRITION: — (STRUCTURE OF FUNGI )Bread moulds are heterotrophs obtaining their nutrition from the
substratum on which they grow. The growing hyphae penetrate into the substratum on which they grow and
secrete enzymes. The enzymes break down organic materials like protein, lipids, and polysaccharides and
digest they food outside the body of fungus. It is called extracellular digestion. The digested food is absorbed
by the hyphae and is stored in the form of glycogen and oil droplets. ( rhizopus and mucor diagram)
RESPIRATION: In mould the mode of respiration is always aerobic i.e. fungus oxidises food in the presence
of oxygen. To get oxygen from air, the bread mould always grows on the exposed surface.
REPRODUCTION: Reproduction is both asexul and sexual in both Mucor and Rhizopus. Asexul
reproduction takes place by formation of non- motile spores while sexual reproduction takes place by
conjugation of isogametes or similar gametes. ( STRUCTURE OF FUNGI)
3. Fig#3 diagram of asexual reproduction of fungi
Fig#4 diagram og sexual reproduction of fungi
B) YEASTS: Yeasts are unicellular fungi of the genus Saccharomyce Yeast cell is very simple in structure
and oval in shape. It has a distinct cell wall, nucleus and nucleolus. The cytoplasm is granular and consists
of glycogen and oil globules. ( STRUCTURE OF FUNGI )
RESPIRATION:- ( STRUCTURE OF FUNGI)
Yeast respires anaerobically without using oxygen and produces carbon dioxide and ethyl alcohol. This is
called fermentation.
C6 H12 O6 ——– C2H5OH+ 2CO2+ 2 ATP ( structure of mucor)
REPRODUCTION:- ( STRUCTURE OF FUNGI)
Yeasts reproduce asexually by budding. Sexual reproduction takes place by conjugation.
4. Fig#5 diagram of a Yeast cell and its reproduction by budding
USE:- Yeasts have several uses –
a) yeasts are used in the production of bread, beer and wine.
b) they are used as nutritional supplements as they contain vitamin B and vitamin E.
C) MUSHROOMS:- Mushrooms are the largest of all fungi. These can be seen in abundance during rainy
season on damp logs , walls and on the ground. Mushrooms are umbrella-like structures. On the underside
of the cap are many gills radiating put from the centre to the margin. ( rhizopus and mucor diagram)
Mushrooms are important food. These are cultivated on a commercial scale for their nutritional value as they
rich in vitamin and proteins. Agaricus, Pennybun, Button mushrooms are ediblisjroom.
However, there are certain field mushrooms such as fly agaric and death caps( Amantia) which are deadly
poisonous and should not be eaten. (STRUCTURE OF FUNGI)
STEPS IN MUSHROOM CULTIVATION ( hyphae that grow into
a cottony mass)
5. Fig#6 diagram of Mushroom
Before mushroom cultivation we have to be ready in three ways.
a) Growing season:- In plain land winter is the best time for growing button mushroom. In hilly area
mushrooms can be cultivated through out the year.
b) Composting:- The compost is prepared by mixing the following ingredients in a certain proportion.
i) wheat or paddy straw
ii) chicken manure
iii) Ammonium sulphate
iv) urea
All the ingredients are mixed thoroughly on wetted staw . The compost which has a dark brown colour is kept
at about 50°C for about one week.
c) Mushroom house:- It should be properly ventilated.
PROCESS OF CULTIVATION
i) Filling:- The compost is filled in trays.
ii) Spawning:- At first mycelium of the selected mushroom is introduced in the compost. After spawning, trays
are arranged one above another in 4-5 tiers and left for a couple of days.
6. Fig#7 diagram of different stages in the development of Mushroom
iii) Casing:- A thin layar of soli is laid about 3cm over the compost surface. It gives support to the mushrooms
, provides humidity and regulates the temperature. ( simple mucor diagram)
iv) Cropping and harvesting:- After 15-20 day’s of casing , first crop of pin heads is visible. Small white
button stage develops 5-6 days after pin head stage. After that full grown mushrooms are harvested.
v) Preservation:- Since mushrooms are highly perishable, they can be preserved in proper way. They must
be kept by vacuum cooling. They must be kept freezed in dry condition. Drying should be done by solutions
of citric acid, ascorbic acid and brine.
PRECAUTIONS TAKEN IN MUSHROOM CULTIVATION
*There should be proper arrangement for circulation of air in the mushroom house .
* A temperature of 25⁰C for three days should maintained.
* After that a temperature of 16-18⁰C should be maintained.
DIFFERENCES IN BACTERIA AND FUNGUS
KEY POINT BACTERIA FUNGI
1) cellular organization unicellular multicellular
2)cell wall Made up of peptidoglycan Made up of chitin
3)nutrition
Autotrophic as well as
heterotrophic
heterotrophic
4)respiration Aerobic or anaerobic aerobic
5) reproduction
Asexual by fission; sexual by
conjugation
Aexual by budding, spore
formation; sexual by
conjugation
FOOD PRESERVATION (STRUCTURE OF FUNGI)
7. Most of the edible products easily perish if not preserved because most of them contain high moisture. Here
are some methods for food preservation.
1) HIGH TEMPERATURE OR STERILISATION:– By this process bacteria are killed by using steam, at very
high temperature, pressure, in autoclave or in pressure cooker. Canned food is preserved by this process.
2) DEHYDRATION OR DRYING:– By this process moisture or water content is dried which helps to stop
growth of microorganisms. Grains are preserved by this process. ( STRUCTURE OF FUNGI)
3) SALTING:– It is very old process. By this process plasmolysis of living tissue is done. This process is also
known as ” curing”. Fish, meat, pickles,are preserved by this process.
4) PASTEURIZATION:– It is a technique but which milk is sterilised. By this process milk is heated upto
65°C for 30 minutes and then chilled quickly. By this process many germs causing typhoid, cholera, and
diptheria are killed.
5) IRRADIATION:– It is the the latest technology of food preservation. By this process food is exposed to
gamma rays or x- rays to kill food spoiling bacteria. Packed meat, fish, vegetables can be preserved by this
process.
6) REFRIGERATION:– Chilling of milk and keeping vegetables, fruits, fish and meat, in a cold storage are
popular method of preservation. Frozen fruits and vegetables retain their flavour and freshness for a long
time. ( STRUCTURE OF FUNGI)
7) PRESERVATIVES:– Addition of preservatives in food help in plasmolysis and death of microorganisms.
Some popular preservatives are vinegar, salt, mustard oil, citric acid which are organic preservatives also.
Benjoic acid, sodium benzoate, ascorbic acid and propionic acid are chemical preservatives.