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P.Tarrats, M. Rieradevall, N. Prat,
M.P. Mata, M. Morellón, J.Vegas,
J. Sánchez, B.L.Valero, A. Moreno
Geneva
5-10 July 2015
: Global Change in Mountain Lakes
Study and understand the present to explain the past
 “Paleoecological reconstructions using biological registers need
a good knowledge of the actual ecology and autoecology of the
species” (Birks and Birks, 2004).
 “It is important to combine paleoecological data with those
obtained from instrumental series and collected from ecological
techniques used in the study of actual ecosystems conditions”
(Peng et al., 2011)
CHIRONOMIDAE
 What?
 Insecta:Diptera
 Aquatics and semi-aquatic
 Larvae inhabit lake’s benthos
 In paleo: larvae head capsules
 Why?
 High taxonomic diversity and abundances
 The presence of different species reflects different
ecological conditions
 Taxonomy (identification): 
 Preservation (Paleo): 
Chironomid life cycle and larvae
 To characterize the modern chironomid community of Enol Lake.
 To evaluate which factors are responsible of changes in
community:
 Abundance
 Composition
We aim to study and understand the actual
Chironomidae community in order to improve the
interpretation of the chironomid subfossil record
Enol Lake
Location North Spain (Asturias)
Picos de Europa National Park
Origin Glacial
Altitude 1070 m asl
Max depth 22 m
Surface Area 12.2 ha
Location map of Enol Lake
Lake type Warm monomictic
Trophic state Oligotrophic
Lake water chemistry
Hard water HCO3
- & Ca
Alkaline
Low nutrient
contents
NO3
2-, NO2-
NH4+ &PO4
3-
Enol Lake
0
2
4
6
8
10
12
14
16
18
20
22
0 25 50 75 100 125 150
Depth(m)
DO (%)
May
July
September
November
0
2
4
6
8
10
12
14
16
18
20
22
5 10 15 20 25
Depth(m)
T (ºC)
May
July
September
November
No depth patterns Temperature and DO profiles of 4 samplings (May, July,
September and November) of 2013 in Enol Lake. Project
CLAM-1 (unpublished data)
 8 fieldwork samplings in 2013 and 2014: May, July,
September and November.
 Depth transect: samples every 2m of depth, 3 replicates
 Profundal zone: Ekman grab (15cm2)
 Littoral zone: kick-sampling (1m2)
Profile of Enol Lake showing
sampling metods and Ekman grab.
 A total of 204 samples were analyzed
Samples cleaning
and sediment
sieving
Chironomidae
larvae morphotype
classification
Macroinvertebrate
sorting (Family
level)
Chironomidae
larvae mounting
and identification
-KOH digestion
-Mounting in slides
-Identification under
a microscope (400x)
 A total of 14,248 Chironomidae larvae were identified
 26 species, 4 sub-families:
 Chironominae
▪ Tribe Chironomini: 11 species
▪ TribeTanytarsini: 4 species
 Orthocladiinae: 7 species
 Tanypodinae: 3 species
 Prodiamesinae: 1 specie
 Rare taxa (<2% in at least 2 samples) were removed in the
data analysis 16 species
Chironomus plumosus, Einfeldia pagana, Procladius choreus and Paratanytarsus bituberculatus from Enol Lake.
Profundal Littoral
Are there significant differences between… (ANOSIMTest)
•Years  p-value=0.57
• Samplings  p-value=0.26
•Depths  p-value= 0.01 R= 0.6
DCA length of gradients <3
RedundancyAnalysis (RDA)
Environmental variables:
•Temperature
• Dissolved Oxygen
• pH
• Depth
• Chara
Species
matrix
Stability of the community
Littoral
zone
Profundal
zone
Chara
zone
RDA Axis 1
RDAAxis2
RDA analysis of Enol Lake data. Axis 1 explains the
29.9% of species variance and the 65.1 % of species –
environment relation variance. Axis 2 explains the
10.3% of species variance and the 22.6% of species-
environment relation variance.
Chara-related taxa
Always high DO values
Profundal taxa
Always no Chara and low DO
Littoral taxa and sub-littoral taxa
-Great variability in Chara
-High DO values, although less than
in Chara-related taxa
Chara and Oxygen main drivers
 The community is quite stable: there are not significant
differences along the year and between years.
 Changes in the Chironomidae community composition and
abundance are mainly driven by the presence of Chara and
changes in the DO content .
 This results will allow us to improve the interpretation and
inference of the paleo record.
▪ Analyse the recent paleo record (1cm of sediment) at every depth to
understand the deposition of the chironomid head capsules.
▪ Analyse two sediment cores .
▪ Apply what we are learning from these studies when interpreting
the subfossil record.

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Chironomid community dynamics in Enol Lake (Picos de Europa National Park, Spain)

  • 1. P.Tarrats, M. Rieradevall, N. Prat, M.P. Mata, M. Morellón, J.Vegas, J. Sánchez, B.L.Valero, A. Moreno Geneva 5-10 July 2015
  • 2. : Global Change in Mountain Lakes Study and understand the present to explain the past  “Paleoecological reconstructions using biological registers need a good knowledge of the actual ecology and autoecology of the species” (Birks and Birks, 2004).  “It is important to combine paleoecological data with those obtained from instrumental series and collected from ecological techniques used in the study of actual ecosystems conditions” (Peng et al., 2011)
  • 3. CHIRONOMIDAE  What?  Insecta:Diptera  Aquatics and semi-aquatic  Larvae inhabit lake’s benthos  In paleo: larvae head capsules  Why?  High taxonomic diversity and abundances  The presence of different species reflects different ecological conditions  Taxonomy (identification):   Preservation (Paleo):  Chironomid life cycle and larvae
  • 4.  To characterize the modern chironomid community of Enol Lake.  To evaluate which factors are responsible of changes in community:  Abundance  Composition We aim to study and understand the actual Chironomidae community in order to improve the interpretation of the chironomid subfossil record
  • 5. Enol Lake Location North Spain (Asturias) Picos de Europa National Park Origin Glacial Altitude 1070 m asl Max depth 22 m Surface Area 12.2 ha Location map of Enol Lake
  • 6. Lake type Warm monomictic Trophic state Oligotrophic Lake water chemistry Hard water HCO3 - & Ca Alkaline Low nutrient contents NO3 2-, NO2- NH4+ &PO4 3- Enol Lake 0 2 4 6 8 10 12 14 16 18 20 22 0 25 50 75 100 125 150 Depth(m) DO (%) May July September November 0 2 4 6 8 10 12 14 16 18 20 22 5 10 15 20 25 Depth(m) T (ºC) May July September November No depth patterns Temperature and DO profiles of 4 samplings (May, July, September and November) of 2013 in Enol Lake. Project CLAM-1 (unpublished data)
  • 7.  8 fieldwork samplings in 2013 and 2014: May, July, September and November.  Depth transect: samples every 2m of depth, 3 replicates  Profundal zone: Ekman grab (15cm2)  Littoral zone: kick-sampling (1m2) Profile of Enol Lake showing sampling metods and Ekman grab.
  • 8.  A total of 204 samples were analyzed Samples cleaning and sediment sieving Chironomidae larvae morphotype classification Macroinvertebrate sorting (Family level) Chironomidae larvae mounting and identification -KOH digestion -Mounting in slides -Identification under a microscope (400x)
  • 9.  A total of 14,248 Chironomidae larvae were identified  26 species, 4 sub-families:  Chironominae ▪ Tribe Chironomini: 11 species ▪ TribeTanytarsini: 4 species  Orthocladiinae: 7 species  Tanypodinae: 3 species  Prodiamesinae: 1 specie  Rare taxa (<2% in at least 2 samples) were removed in the data analysis 16 species Chironomus plumosus, Einfeldia pagana, Procladius choreus and Paratanytarsus bituberculatus from Enol Lake.
  • 11. Are there significant differences between… (ANOSIMTest) •Years  p-value=0.57 • Samplings  p-value=0.26 •Depths  p-value= 0.01 R= 0.6 DCA length of gradients <3 RedundancyAnalysis (RDA) Environmental variables: •Temperature • Dissolved Oxygen • pH • Depth • Chara Species matrix Stability of the community
  • 12. Littoral zone Profundal zone Chara zone RDA Axis 1 RDAAxis2 RDA analysis of Enol Lake data. Axis 1 explains the 29.9% of species variance and the 65.1 % of species – environment relation variance. Axis 2 explains the 10.3% of species variance and the 22.6% of species- environment relation variance.
  • 13. Chara-related taxa Always high DO values Profundal taxa Always no Chara and low DO Littoral taxa and sub-littoral taxa -Great variability in Chara -High DO values, although less than in Chara-related taxa Chara and Oxygen main drivers
  • 14.  The community is quite stable: there are not significant differences along the year and between years.  Changes in the Chironomidae community composition and abundance are mainly driven by the presence of Chara and changes in the DO content .  This results will allow us to improve the interpretation and inference of the paleo record. ▪ Analyse the recent paleo record (1cm of sediment) at every depth to understand the deposition of the chironomid head capsules. ▪ Analyse two sediment cores . ▪ Apply what we are learning from these studies when interpreting the subfossil record.