The document compares the metabolizable energy (ME) values of different poultry feeds estimated using a regression equation and feeding trials. It finds that all commercially produced feeds have lower ME than National Research Council standards. ME values calculated from feeding trials are similar to commercial feed values but different from values estimated using the regression equation. The regression equation method is less accurate but more effective than calculation from feeding trials.

2.  Comparison of metabolizable energy values of
different types of poultry feeds estimated using
regression equation and feeding trials
 Comparison of metabolizable energy values of
commercially produce poultry feeds with National
Research Council standards

3.  Estimation of metabolizable energy using regression equation
• Feeds were proximately analyzed
•Metabolizable energy of poultry feeds were estimated using
proximate analyzed data referring following equation
• ME (KJ/kg DM) = (31.97fat +14.14 CP +14.48 NFE - 18.37
CF)

4.  Calculation of metabolizable energy using in vivo (bio
assay)trials
 Twelve weeks old 45 Japanese quails (Coturnix coturnix japonica) were housed in 15 cages
 Five experimental feeds were assigned to 15 groups of birds with three replicates
 One week was allocated for five type of feeds
 preliminary period extended for 3 days
 Samples of excreta collected from each replicate group during four consecutive days
 Analyze dry matter(DM), acid insoluble ash (AIA), and gross energy content(GE) of feed and
excreta
 Gross energy content of feed and dried excreta samples were measured using bomb calorimeter
(IKA C 700Calorimeter)
 Metabolizable energy values of the eighteen feed samples were calculated using following equation
ME feed (kcal/kg) = GE feed×
𝑨𝑰𝑨𝒇𝒆𝒆𝒅
𝑨𝑰𝑨𝒆𝒙𝒄𝒓𝒆𝒕𝒂
×
𝑮𝑬𝒆𝒙𝒄𝒓𝒆𝒕𝒂
𝑮𝑬𝒇𝒆𝒆𝒅

5. 0
500
1000
1500
2000
2500
3000
3500
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18
MetabolizableEnergy(ME)Kcal/KgDM
feed type
1.Comparison of metabolizable energy values with NRC
classification
ME
Commercial
feed
ME NRC
 All commercially produced poultry feeds
contained low amount metabolizable
energy than NRC recommendations
 It was not depend on the brand of feed
 Value difference of feed with
recommendation change according to the
type of feed( broiler or layer feed)
 Statistically analyzed using Chi-square
test
 Commercial poultry feeds ME values
significant with (P<0.05) NRC values
 There is association between NRC and
commercial poultry feeds

6. 1500
2000
2500
3000
3500
1 2 3 4 5 6 7 8 9 101112131415161718
MetabolizableenergyKcal/KgDM
poultry feed
ME estimated ME calculated
0
500
1000
1500
2000
2500
3000
3500
4000
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18
Metabolizableenergy(Kcal/KgDM)
Poultry feed
ME calculated ME Commercial feed
Comparison of ME commercial feed with ME
calculated values values
Comparison of ME estimated values with ME
calculated values

7.  Equality of variance of,
ME calculated and ME commercial is not significant
(P>0.05)
Therefore Equality of variance of ME calculated and ME
commercial are accepted
 Two-sample T-Test,
ME calculated and ME commercial is not significant (p>0.05)
Therefore means of ME calculated and ME commercial are
same

8. Comparison with ME commercial feed with ME estimated
values values
 Equality of variance of,
ME estimated and ME
commercial is not significant
(P>0.05)
Therefore Equality of
variance of ME estimated and
ME commercial are accepted
 Two-sample T-Test,
ME estimated and ME
commercial is not significant
(p>0.05)
Therefore means of ME
estimated and ME commercial
are same
0
500
1000
1500
2000
2500
3000
3500
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18
Metabolizableenergy(Kcal/KgDM)
poultry feed
ME estimated ME Commercial feed

9. 2000
2100
2200
2300
2400
2500
2600
2700
2800
2900
3000
ME esti ME
comm
ME cal ME
comm
Meatabolizableenergy(Kcal/KgDM)
ME
esti
ME
com
m
ME
cal
ME
com
m
Comparison of ME commercial with ME estimated and
ME calculated values values
360034003200300028002600240022002000
3300
3200
3100
3000
2900
2800
2700
2600
2500
MEcalculated(Kcal/kgDM)
MEestimated(Kcal/kgDM)
Estimated and calculated values with
correlation analysis
r =0.607 ( P<0.05)

10.  All commercially produced poultry feeds contained low amount metabolizable energy than
NRC recommendations
 It was not depend on the brand of feed
 Value difference of feed with recommendation change according to the type of feed( broiler or
layer feed)
 Statistically analyzed using Chi-square test
 Commercial poultry feeds ME values significant with (P<0.05) NRC values
 There is association between NRC and commercial poultry feedsBut those two methods give
approximately accurate values for the evaluating energy of compound feeds of poultry.
 New researches should be directed to need various needs of birds in various conditions
 Such as different phase of growth, genetic and processing of feed.
 Metabolizable energy estimation using regression equation method not
similar to values obtained using with the calculation using feeding trials
 Regression equation methods is more effective than calculating using
feeding trials