Shirt P was washed at 150C, shirt Q at 350C, and shirt R at 650C. Shirt R, washed at the highest temperature, had the stain completely removed. Suggested changes to improve removal include washing at a higher temperature or adding more enzyme. The likely enzyme used is amylase, and a protease enzyme would be best for removing blood stains. Enzymes remove food stains by catalyzing reactions that break down the components of the stain.