1. Gene expression in eukaryotic cells is regulated through the interaction of cis-acting DNA elements like promoters and enhancers with trans-acting transcription factors. Promoters contain core elements and proximal elements that bind transcription factors to initiate low-level or high-level transcription respectively. Enhancers can regulate genes from long distances by looping DNA.
2. Chromatin structure and epigenetic modifications also regulate gene expression. Genes packaged in condensed heterochromatin are generally transcriptionally silent, while genes in open euchromatin can be expressed. Methylation of CpG islands can silence genes by blocking transcription factor binding or inducing heterochromatin formation. Histone modifications like acetylation also regulate gene
Eukaryotic gene regulation PART II 2013Jill Howlin
This document provides an overview of techniques for analyzing gene expression and regulation. It discusses RNA sequencing (RNA-seq) and its applications like whole transcriptome analysis and mRNA sequencing. It also covers chromatin immunoprecipitation followed by sequencing (ChIP-seq) to map DNA-protein interactions genome-wide. Microarrays are mentioned as earlier techniques that were replaced by high-throughput sequencing methods allowing for comprehensive analysis of transcription factors, histone modifications, and regulatory elements without prior sequence knowledge.
Gene expression in eukaryotes is regulated at multiple levels, including transcription, RNA processing, mRNA degradation, and protein degradation. Transcription is controlled through chromatin remodeling, transcription factors, enhancers, promoters, and repressors. Alternative RNA splicing allows different mRNAs and proteins to be produced from the same gene. Non-coding RNAs like miRNAs can degrade mRNAs or block translation. Proteins are degraded by proteasomes and ubiquitin pathways. Microarrays allow comparison of gene expression between cell types by detecting differences in mRNA levels.
The document discusses gene expression and its regulation in eukaryotic cells. It states that while all somatic cells contain the same genetic information, differential gene expression gives rise to different cell types through the control of which genes are expressed. Gene expression in eukaryotes is regulated at multiple levels, including epigenetic, transcriptional, post-transcriptional, translational and post-translational levels. Cancer arises due to mutations that modify cell cycle control genes, causing uncontrolled cell growth. Gene expression changes in cancer can be caused by mutations, epigenetic alterations, or changes in transcriptional or post-transcriptional regulation.
The document discusses genes in prokaryotes. It defines key terms like gene, prokaryotic gene, and operon. It explains that prokaryotic genes consist of a promoter region, RNA coding sequence, and terminator region. Gene expression involves transcription and translation processes that occur in the cytoplasm. Gene regulation is achieved through repressible and inducible operons like the trp and lac operons, which are controlled by repressor and activator proteins that bind to DNA in response to environmental stimuli.
Cell surface and intrcellular receptorsEstherShoba1
Cell surface and intracellular receptors play important roles in signal transduction. There are two main types of receptors - internal receptors located in the cytoplasm that directly influence gene expression, and cell surface receptors that span the plasma membrane and convert extracellular signals into intracellular signals. Cell surface receptors include enzyme-linked receptors with intracellular enzyme domains, ion channel-linked receptors that open channels for ion flow, and G-protein-linked receptors that activate intracellular G-proteins to transmit signals. Defects in cell surface receptors can cause diseases.
Eukaryotic gene regulation PART II 2013Jill Howlin
This document provides an overview of techniques for analyzing gene expression and regulation. It discusses RNA sequencing (RNA-seq) and its applications like whole transcriptome analysis and mRNA sequencing. It also covers chromatin immunoprecipitation followed by sequencing (ChIP-seq) to map DNA-protein interactions genome-wide. Microarrays are mentioned as earlier techniques that were replaced by high-throughput sequencing methods allowing for comprehensive analysis of transcription factors, histone modifications, and regulatory elements without prior sequence knowledge.
Gene expression in eukaryotes is regulated at multiple levels, including transcription, RNA processing, mRNA degradation, and protein degradation. Transcription is controlled through chromatin remodeling, transcription factors, enhancers, promoters, and repressors. Alternative RNA splicing allows different mRNAs and proteins to be produced from the same gene. Non-coding RNAs like miRNAs can degrade mRNAs or block translation. Proteins are degraded by proteasomes and ubiquitin pathways. Microarrays allow comparison of gene expression between cell types by detecting differences in mRNA levels.
The document discusses gene expression and its regulation in eukaryotic cells. It states that while all somatic cells contain the same genetic information, differential gene expression gives rise to different cell types through the control of which genes are expressed. Gene expression in eukaryotes is regulated at multiple levels, including epigenetic, transcriptional, post-transcriptional, translational and post-translational levels. Cancer arises due to mutations that modify cell cycle control genes, causing uncontrolled cell growth. Gene expression changes in cancer can be caused by mutations, epigenetic alterations, or changes in transcriptional or post-transcriptional regulation.
The document discusses genes in prokaryotes. It defines key terms like gene, prokaryotic gene, and operon. It explains that prokaryotic genes consist of a promoter region, RNA coding sequence, and terminator region. Gene expression involves transcription and translation processes that occur in the cytoplasm. Gene regulation is achieved through repressible and inducible operons like the trp and lac operons, which are controlled by repressor and activator proteins that bind to DNA in response to environmental stimuli.
Cell surface and intrcellular receptorsEstherShoba1
Cell surface and intracellular receptors play important roles in signal transduction. There are two main types of receptors - internal receptors located in the cytoplasm that directly influence gene expression, and cell surface receptors that span the plasma membrane and convert extracellular signals into intracellular signals. Cell surface receptors include enzyme-linked receptors with intracellular enzyme domains, ion channel-linked receptors that open channels for ion flow, and G-protein-linked receptors that activate intracellular G-proteins to transmit signals. Defects in cell surface receptors can cause diseases.
Genome size, organization,& gene regulation in prokaryotes (lac-operon)Iqra Wazir
Genome size refers to the total amount of DNA in an organism and can vary widely between species. Prokaryotic genomes typically consist of a single circular chromosome between 0.6-10 megabases in length, and sometimes plasmids up to 1.7 megabases. Gene regulation in prokaryotes occurs at the transcriptional level through operons, which contain multiple genes regulated by a single promoter. The lac operon in E. coli contains genes to break down lactose which are regulated by a repressor protein; in the presence of lactose or its isomer allolactose, the repressor detaches from the operator and allows transcription.
Gene expression involves transcription of DNA into mRNA and translation of mRNA into protein. There are two main types of genes - constitutive genes which are continually expressed, and inducible genes which are expressed only when needed. Gene expression is regulated at multiple levels, including transcription, RNA processing, translation, and post-translational modification. Key mechanisms of transcriptional control include operons in prokaryotes and the actions of transcription factors in eukaryotes.
Regulation of gene expression allows organisms to benefit from efficiency, conserving energy and cell size. In prokaryotes, operons regulate groups of genes, turned on or off by repressors, activators, or inducers. Eukaryotes separate transcription and translation, introducing many regulatory mechanisms. These include epigenetic modifications, transcription factors, RNA processing, stability, and translation factors. Cancer arises from dysregulation of genes controlling cell growth, especially tumor suppressors and oncogenes.
1. Gene expression can be regulated positively or negatively at the levels of transcription, RNA processing, translation and protein activity through the actions of regulatory proteins and hormones.
2. Hormones like steroids enter cells and bind nuclear receptors to activate transcription, while peptide hormones signal through cell surface receptors and secondary messengers.
3. Key mechanisms of transcriptional control include chromatin remodeling, DNA methylation, and the binding of transcription factors to regulatory sequences which can either promote or block transcription initiation.
Promoters are regions of DNA that initiate gene transcription. They are located upstream of genes and can range from 100-1000 base pairs long. Promoters contain an RNA polymerase binding site and initiation site to control transcription. There are several types of promoters including constitutive, inducible, tissue-specific, and synthetic promoters. Constitutive promoters induce expression in all tissues, while inducible promoters respond to environmental or chemical factors and tissue-specific promoters function in particular tissues.
Gene regulation in prokaryotes and eukaryotes can occur at multiple levels. In prokaryotes, the lac operon is a classic example of negative gene regulation at the transcription level. The lac operon is induced in the presence of lactose, when the lactose binds to the repressor and inactivates it, allowing transcription. In eukaryotes, gene regulation can occur through transcription factors binding promoter regions to activate transcription, or through displacement factors blocking transcription. Regulation can also occur post-transcriptionally or post-translationally.
This document discusses regulation of gene expression in eukaryotes. It describes six main levels of control: transcription, RNA processing, mRNA transport, mRNA translation, mRNA degradation, and protein degradation. Key differences between prokaryotic and eukaryotic gene expression are explained, such as eukaryotes possessing nuclei and more complex regulation. Examples of short-term regulation including the GAL gene pathway in yeast and hormone response are provided.
This document discusses gene regulation in prokaryotes and eukaryotes. It explains that gene regulation allows cells to only express genes when they are needed. In prokaryotes, gene regulation typically occurs through operons at the transcriptional level. Eukaryotic gene regulation is more complex and can occur through epigenetic, transcriptional, post-transcriptional, translational and post-translational mechanisms. Key methods of regulation include chromatin remodeling, transcription factor binding, RNA processing, mRNA degradation, and protein degradation.
This PowerPoint is applicable for the medical, paramedical, and all the life science students who read the mechanism of gene expression. This is equally useful for teachers as well. This is the comprehensive coverage on the aforementioned topic.
1) Eukaryotic gene expression is regulated at multiple levels including transcription, chromatin structure, post-transcriptional processing, and translation.
2) Regulation allows for adaptation and tissue-specific gene expression during development. Key differences from prokaryotes include the lack of operons and more complex regulation in eukaryotes.
3) Gene expression can be regulated short-term through transcriptional control, as seen in yeast galactose-utilizing genes, or long-term for development through mechanisms like chromatin remodeling.
Promoters are regions of DNA located near gene transcription start sites that initiate gene transcription. There are two main types of promoters based on their CG content: high CG content promoters associated with constitutively expressed genes, and low CG content promoters associated with tissue-specific genes. Bidirectional promoters simultaneously control pairs of genes on opposite DNA strands that are coexpressed. Promoters can be constitutive and initiate transcription in all tissues, inducible and require biotic/abiotic factors, or tissue-specific and active only in certain tissues.
This document discusses the control of gene expression in prokaryotes and eukaryotes. In prokaryotes, genes are organized into operons and expressed through positive or negative transcriptional control when needed for metabolic pathways. The lac operon is negatively regulated by the lac repressor. In eukaryotes, general transcription factors and specific transcription factors regulate transcription, and chromatin structure and post-transcriptional mechanisms like alternative splicing further control gene expression.
Epigenetics refers to chemical modifications of DNA and histones that regulate gene expression without altering the underlying DNA sequence. There are several key epigenetic mechanisms:
1. Histone modifications such as acetylation and methylation can open or close chromatin and thereby activate or repress genes.
2. DNA methylation involves adding methyl groups to cytosine bases in CpG islands in gene promoters, repressing gene expression.
3. Non-coding RNAs can regulate genes as signals, decoys, guides, or scaffolds.
Epigenetic modifications are important for normal development and can be influenced by environmental factors. They regulate processes like X-chromosome inactivation and genomic imprinting, where genes are expressed based
Bacterial genetics covers the structure and function of bacterial genetic material, DNA and RNA. It discusses DNA replication, transcription, translation and protein synthesis. It defines different types of mutations like point mutations, frameshift mutations, deletions and their effects. It also describes three main mechanisms of genetic transfer in bacteria - transformation, transduction and conjugation. Transformation involves uptake of naked DNA by competent bacteria. Transduction involves transfer of DNA between bacteria by bacteriophages. Conjugation involves transfer of plasmids containing conjugation genes between "donor" and "recipient" bacteria through cell-to-cell contact.
Gene expression in eukaryotes is controlled at multiple levels, including chromatin structure, transcription, RNA processing, and translation. Chromatin structure determines if genes are transcriptionally active or inactive. Transcription is regulated by the interaction of promoters, transcription factors, and enhancers. RNA processing controls splicing and transport of mRNA. Finally, translation and post-translational modifications further regulate gene expression. Overall, eukaryotic gene expression is tightly controlled through complex mechanisms at the chromatin, transcription, RNA, translation, and protein levels.
The presentation illustrates the basic modes of cell signalling pathways for undergraduate students. It mentions variety of examples of cell signalling with different receptors, ligands and target molecules
1. The document outlines different types of cellular receptors including cell surface receptors like G protein-coupled receptors and tyrosine kinase receptors, as well as intracellular receptors.
2. It provides examples of hormone signaling pathways, noting that hormones activate receptors which then trigger intracellular second messenger systems like cAMP, calcium, or phosphorylation to produce cellular effects.
3. The mechanism of insulin signaling is described as an example of receptor tyrosine kinase activation, showing how insulin binding leads to phosphorylation of intracellular targets and downstream effects on processes like glucose uptake and fat/protein synthesis.
Genome size, organization,& gene regulation in prokaryotes (lac-operon)Iqra Wazir
Genome size refers to the total amount of DNA in an organism and can vary widely between species. Prokaryotic genomes typically consist of a single circular chromosome between 0.6-10 megabases in length, and sometimes plasmids up to 1.7 megabases. Gene regulation in prokaryotes occurs at the transcriptional level through operons, which contain multiple genes regulated by a single promoter. The lac operon in E. coli contains genes to break down lactose which are regulated by a repressor protein; in the presence of lactose or its isomer allolactose, the repressor detaches from the operator and allows transcription.
Gene expression involves transcription of DNA into mRNA and translation of mRNA into protein. There are two main types of genes - constitutive genes which are continually expressed, and inducible genes which are expressed only when needed. Gene expression is regulated at multiple levels, including transcription, RNA processing, translation, and post-translational modification. Key mechanisms of transcriptional control include operons in prokaryotes and the actions of transcription factors in eukaryotes.
Regulation of gene expression allows organisms to benefit from efficiency, conserving energy and cell size. In prokaryotes, operons regulate groups of genes, turned on or off by repressors, activators, or inducers. Eukaryotes separate transcription and translation, introducing many regulatory mechanisms. These include epigenetic modifications, transcription factors, RNA processing, stability, and translation factors. Cancer arises from dysregulation of genes controlling cell growth, especially tumor suppressors and oncogenes.
1. Gene expression can be regulated positively or negatively at the levels of transcription, RNA processing, translation and protein activity through the actions of regulatory proteins and hormones.
2. Hormones like steroids enter cells and bind nuclear receptors to activate transcription, while peptide hormones signal through cell surface receptors and secondary messengers.
3. Key mechanisms of transcriptional control include chromatin remodeling, DNA methylation, and the binding of transcription factors to regulatory sequences which can either promote or block transcription initiation.
Promoters are regions of DNA that initiate gene transcription. They are located upstream of genes and can range from 100-1000 base pairs long. Promoters contain an RNA polymerase binding site and initiation site to control transcription. There are several types of promoters including constitutive, inducible, tissue-specific, and synthetic promoters. Constitutive promoters induce expression in all tissues, while inducible promoters respond to environmental or chemical factors and tissue-specific promoters function in particular tissues.
Gene regulation in prokaryotes and eukaryotes can occur at multiple levels. In prokaryotes, the lac operon is a classic example of negative gene regulation at the transcription level. The lac operon is induced in the presence of lactose, when the lactose binds to the repressor and inactivates it, allowing transcription. In eukaryotes, gene regulation can occur through transcription factors binding promoter regions to activate transcription, or through displacement factors blocking transcription. Regulation can also occur post-transcriptionally or post-translationally.
This document discusses regulation of gene expression in eukaryotes. It describes six main levels of control: transcription, RNA processing, mRNA transport, mRNA translation, mRNA degradation, and protein degradation. Key differences between prokaryotic and eukaryotic gene expression are explained, such as eukaryotes possessing nuclei and more complex regulation. Examples of short-term regulation including the GAL gene pathway in yeast and hormone response are provided.
This document discusses gene regulation in prokaryotes and eukaryotes. It explains that gene regulation allows cells to only express genes when they are needed. In prokaryotes, gene regulation typically occurs through operons at the transcriptional level. Eukaryotic gene regulation is more complex and can occur through epigenetic, transcriptional, post-transcriptional, translational and post-translational mechanisms. Key methods of regulation include chromatin remodeling, transcription factor binding, RNA processing, mRNA degradation, and protein degradation.
This PowerPoint is applicable for the medical, paramedical, and all the life science students who read the mechanism of gene expression. This is equally useful for teachers as well. This is the comprehensive coverage on the aforementioned topic.
1) Eukaryotic gene expression is regulated at multiple levels including transcription, chromatin structure, post-transcriptional processing, and translation.
2) Regulation allows for adaptation and tissue-specific gene expression during development. Key differences from prokaryotes include the lack of operons and more complex regulation in eukaryotes.
3) Gene expression can be regulated short-term through transcriptional control, as seen in yeast galactose-utilizing genes, or long-term for development through mechanisms like chromatin remodeling.
Promoters are regions of DNA located near gene transcription start sites that initiate gene transcription. There are two main types of promoters based on their CG content: high CG content promoters associated with constitutively expressed genes, and low CG content promoters associated with tissue-specific genes. Bidirectional promoters simultaneously control pairs of genes on opposite DNA strands that are coexpressed. Promoters can be constitutive and initiate transcription in all tissues, inducible and require biotic/abiotic factors, or tissue-specific and active only in certain tissues.
This document discusses the control of gene expression in prokaryotes and eukaryotes. In prokaryotes, genes are organized into operons and expressed through positive or negative transcriptional control when needed for metabolic pathways. The lac operon is negatively regulated by the lac repressor. In eukaryotes, general transcription factors and specific transcription factors regulate transcription, and chromatin structure and post-transcriptional mechanisms like alternative splicing further control gene expression.
Epigenetics refers to chemical modifications of DNA and histones that regulate gene expression without altering the underlying DNA sequence. There are several key epigenetic mechanisms:
1. Histone modifications such as acetylation and methylation can open or close chromatin and thereby activate or repress genes.
2. DNA methylation involves adding methyl groups to cytosine bases in CpG islands in gene promoters, repressing gene expression.
3. Non-coding RNAs can regulate genes as signals, decoys, guides, or scaffolds.
Epigenetic modifications are important for normal development and can be influenced by environmental factors. They regulate processes like X-chromosome inactivation and genomic imprinting, where genes are expressed based
Bacterial genetics covers the structure and function of bacterial genetic material, DNA and RNA. It discusses DNA replication, transcription, translation and protein synthesis. It defines different types of mutations like point mutations, frameshift mutations, deletions and their effects. It also describes three main mechanisms of genetic transfer in bacteria - transformation, transduction and conjugation. Transformation involves uptake of naked DNA by competent bacteria. Transduction involves transfer of DNA between bacteria by bacteriophages. Conjugation involves transfer of plasmids containing conjugation genes between "donor" and "recipient" bacteria through cell-to-cell contact.
Gene expression in eukaryotes is controlled at multiple levels, including chromatin structure, transcription, RNA processing, and translation. Chromatin structure determines if genes are transcriptionally active or inactive. Transcription is regulated by the interaction of promoters, transcription factors, and enhancers. RNA processing controls splicing and transport of mRNA. Finally, translation and post-translational modifications further regulate gene expression. Overall, eukaryotic gene expression is tightly controlled through complex mechanisms at the chromatin, transcription, RNA, translation, and protein levels.
The presentation illustrates the basic modes of cell signalling pathways for undergraduate students. It mentions variety of examples of cell signalling with different receptors, ligands and target molecules
1. The document outlines different types of cellular receptors including cell surface receptors like G protein-coupled receptors and tyrosine kinase receptors, as well as intracellular receptors.
2. It provides examples of hormone signaling pathways, noting that hormones activate receptors which then trigger intracellular second messenger systems like cAMP, calcium, or phosphorylation to produce cellular effects.
3. The mechanism of insulin signaling is described as an example of receptor tyrosine kinase activation, showing how insulin binding leads to phosphorylation of intracellular targets and downstream effects on processes like glucose uptake and fat/protein synthesis.
Similar to Ch20-1 euk gene reg.pdfhaywbwklwnwyw7wjo (20)
Gender and Mental Health - Counselling and Family Therapy Applications and In...PsychoTech Services
A proprietary approach developed by bringing together the best of learning theories from Psychology, design principles from the world of visualization, and pedagogical methods from over a decade of training experience, that enables you to: Learn better, faster!
LAND USE LAND COVER AND NDVI OF MIRZAPUR DISTRICT, UPRAHUL
This Dissertation explores the particular circumstances of Mirzapur, a region located in the
core of India. Mirzapur, with its varied terrains and abundant biodiversity, offers an optimal
environment for investigating the changes in vegetation cover dynamics. Our study utilizes
advanced technologies such as GIS (Geographic Information Systems) and Remote sensing to
analyze the transformations that have taken place over the course of a decade.
The complex relationship between human activities and the environment has been the focus
of extensive research and worry. As the global community grapples with swift urbanization,
population expansion, and economic progress, the effects on natural ecosystems are becoming
more evident. A crucial element of this impact is the alteration of vegetation cover, which plays a
significant role in maintaining the ecological equilibrium of our planet.Land serves as the foundation for all human activities and provides the necessary materials for
these activities. As the most crucial natural resource, its utilization by humans results in different
'Land uses,' which are determined by both human activities and the physical characteristics of the
land.
The utilization of land is impacted by human needs and environmental factors. In countries
like India, rapid population growth and the emphasis on extensive resource exploitation can lead
to significant land degradation, adversely affecting the region's land cover.
Therefore, human intervention has significantly influenced land use patterns over many
centuries, evolving its structure over time and space. In the present era, these changes have
accelerated due to factors such as agriculture and urbanization. Information regarding land use and
cover is essential for various planning and management tasks related to the Earth's surface,
providing crucial environmental data for scientific, resource management, policy purposes, and
diverse human activities.
Accurate understanding of land use and cover is imperative for the development planning
of any area. Consequently, a wide range of professionals, including earth system scientists, land
and water managers, and urban planners, are interested in obtaining data on land use and cover
changes, conversion trends, and other related patterns. The spatial dimensions of land use and
cover support policymakers and scientists in making well-informed decisions, as alterations in
these patterns indicate shifts in economic and social conditions. Monitoring such changes with the
help of Advanced technologies like Remote Sensing and Geographic Information Systems is
crucial for coordinated efforts across different administrative levels. Advanced technologies like
Remote Sensing and Geographic Information Systems
9
Changes in vegetation cover refer to variations in the distribution, composition, and overall
structure of plant communities across different temporal and spatial scales. These changes can
occur natural.
Chapter wise All Notes of First year Basic Civil Engineering.pptxDenish Jangid
Chapter wise All Notes of First year Basic Civil Engineering
Syllabus
Chapter-1
Introduction to objective, scope and outcome the subject
Chapter 2
Introduction: Scope and Specialization of Civil Engineering, Role of civil Engineer in Society, Impact of infrastructural development on economy of country.
Chapter 3
Surveying: Object Principles & Types of Surveying; Site Plans, Plans & Maps; Scales & Unit of different Measurements.
Linear Measurements: Instruments used. Linear Measurement by Tape, Ranging out Survey Lines and overcoming Obstructions; Measurements on sloping ground; Tape corrections, conventional symbols. Angular Measurements: Instruments used; Introduction to Compass Surveying, Bearings and Longitude & Latitude of a Line, Introduction to total station.
Levelling: Instrument used Object of levelling, Methods of levelling in brief, and Contour maps.
Chapter 4
Buildings: Selection of site for Buildings, Layout of Building Plan, Types of buildings, Plinth area, carpet area, floor space index, Introduction to building byelaws, concept of sun light & ventilation. Components of Buildings & their functions, Basic concept of R.C.C., Introduction to types of foundation
Chapter 5
Transportation: Introduction to Transportation Engineering; Traffic and Road Safety: Types and Characteristics of Various Modes of Transportation; Various Road Traffic Signs, Causes of Accidents and Road Safety Measures.
Chapter 6
Environmental Engineering: Environmental Pollution, Environmental Acts and Regulations, Functional Concepts of Ecology, Basics of Species, Biodiversity, Ecosystem, Hydrological Cycle; Chemical Cycles: Carbon, Nitrogen & Phosphorus; Energy Flow in Ecosystems.
Water Pollution: Water Quality standards, Introduction to Treatment & Disposal of Waste Water. Reuse and Saving of Water, Rain Water Harvesting. Solid Waste Management: Classification of Solid Waste, Collection, Transportation and Disposal of Solid. Recycling of Solid Waste: Energy Recovery, Sanitary Landfill, On-Site Sanitation. Air & Noise Pollution: Primary and Secondary air pollutants, Harmful effects of Air Pollution, Control of Air Pollution. . Noise Pollution Harmful Effects of noise pollution, control of noise pollution, Global warming & Climate Change, Ozone depletion, Greenhouse effect
Text Books:
1. Palancharmy, Basic Civil Engineering, McGraw Hill publishers.
2. Satheesh Gopi, Basic Civil Engineering, Pearson Publishers.
3. Ketki Rangwala Dalal, Essentials of Civil Engineering, Charotar Publishing House.
4. BCP, Surveying volume 1
How to Setup Warehouse & Location in Odoo 17 InventoryCeline George
In this slide, we'll explore how to set up warehouses and locations in Odoo 17 Inventory. This will help us manage our stock effectively, track inventory levels, and streamline warehouse operations.
1. 1
Gene Regulation in Eukaryotes
• All cells in an organism contain all the DNA:
– all genetic info
• Must regulate or control which genes are
turned on in which cells
• Genes turned on determine cells’ function
– E.g.) liver cells express genes for liver enzymes but not
genes for stomach enzymes
2. 2
Proteins act in trans
DNA sites act only in cis
• Trans acting elements (not DNA) can diffuse
through cytoplasm and act at target DNA
sites on any DNA molecule in cell (usually
proteins)
• Cis acting elements (DNA sequences) can
only influence expression of adjacent genes
on same DNA molecule
/27
3. 3
Eukaryotic Promoters
trans-acting proteins control transcription from
class II (RNA pol II) promoters
• Basal factors bind to
the core promoter
– TBP – TATA box binding
protein
– TAF – TBP associated
factors
• RNA polymerase II
binds to basal
factors
Fig. 17.4 a
4. 4
Eukaryotic Promoters
• Promoter proximal elements are required for high
levels of transcription.
• They are further upstream from the start site, usually
at positions between -50 and -500.
• These elements generally function in either
orientation.
• Examples include:
– The CAAT box consensus sequence CCAAT
– The GC box consensus sequence GGGCGG
– Octamer consensus sequence AGCTAAAT
5. 5
Regulatory elements that map near a
gene are cis-acting DNA sequences
• cis-acting elements
– Core Promoter – Basal level expression
• Binding site for TATA-binding protein and associated factors
– Promoter Proximal Elements - True level of expression
• Binding sites for transcription factors
Core
6. 6
Eukaryotic Promoter Elements
• Various combinations of core and proximal elements
are found near different genes.
• Promoter proximal elements are key to gene
expression.
– Activators, proteins important in transcription regulation, are
recognized by promoter proximal elements.
– Housekeeping genes
• used in all cell types for basic cellular functions
• have common promoter proximal elements
• are recognized by activator proteins found in all cells.
– Genes expressed only in some cell types or at particular times have
promoter proximal elements recognized by activator proteins found
only in specific cell types or times.
7. 7
Eukaryotic Enhancer Sequences
• Enhancers are another cis-acting element.
• They are required for maximal transcription of a
gene.
! Enhancers can be upstream or downstream of the transcription
initiation site
! They may modulate from a distance of thousands of base pairs away
from the initiation site.
! Enhancers contain short sequence elements, some similar to
promoter sequences.
! Activators bind these sequences and other protein complexes form,
postulated to bring the enhancer complex close to the promoter and
increasing transcription.
8. 8
Regulatory elements that map near a
gene are cis-acting DNA sequences
• cis-acting elements
– Promoter – very close to gene’s initiation site
– Enhancer
• can lie far way from gene
• Can be reversed
• Augment or repress basal levels of transcription
9. 9
Regulatory elements that act on the promoter or
enhancer sequences are trans-acting factors
• Genes that encode
proteins that interact
directly or indirectly
with target genes cis-
acting elements
– Known genetically as
transcription factors
– Identified by:
• Mapping
• Biochemical studies
to identify proteins
that bind in vitro to
cis-acting elements
10. 10
How do Enhancers work if they are so far
away from the promoter?
• Possible looping of DNA
• Brings transcription factors together
11. 11
Transcription Factors
• Also called activator proteins and silencer proteins
• Bind to promoter, enhancer, and silencer DNA in
specific ways
• Interact with other proteins to activate and increase
transcription as much as 100-fold above basal
levels
– or repress transcription in the case of silencers/repressors
• Two structural domains mediate these functions
– DNA-binding domain
– Transcription-activator domain
12. 12
• Transcriptional
activators bind
to specific
promoters and
enhancers at
specific times to
increase
transcriptional
levels
Fig. 17.5 a
Transcription Factors
14. 14
Some proteins affect transcription without
binding to DNA
• Coactivator –
– binds to and affects activator protein which binds to DNA
– Does not itself bind to DNA
• Corepressors
– binds to and affects silencer/repressor protein which
binds to DNA
– Does not itself bind to DNA
15. 15
Localization of activator domains using
recombinant DNA constructs
• Fusion constructs from
three parts of gene
encoding an activator
protein
• Reporter gene can only
be transcribed if
activator domain is
present in the fusion
construct
• Part B contains
activation domain, but
not part A or C
Fig. 17.6
16. 16
Most eukaryotic activators must form
dimers to function
• Eukaryotic transcription factor protein structure
– Homomers – multimeric proteins composed of identical subunits
– Heteromers – multimeric proteins composed of nonidentical subunits
Fig. 17.7 a
18. 18
Myc-Max system is a regulatory mechanism for
switching between activation and repression
• Myc polypeptide has an activation domain
• Max polypeptide does not have an activation
domain
Fig. 17.10
19. 19
Myc-Max system is a regulatory mechanism for
switching between activation and repression
• Myc cannot form
homodimers or bind
DNA, but has
transactivation domain
• Max homodimers can
bind DNA, but cannot
transactivate (has no
transactivation domain)
• Only Myc-Max
heterodimer can bind
DNA and transactivate
Fig. 17.10
20. 20
Gene Repression results when only the
Max polypeptide is made in the cell
• Gene Activation occurs when both Myc and
Max are made in the cell
•Max prefers Myc as a partner
•Always heterodimerizes if possible
• Gene Repression results when only the
Max polypeptide is made in the cell
•Only homodimerizes when there is no
myc available
23. 23
Role of Chromatin in Gene Regulation
• Two broad classes of chromatin:
– Euchromatin: Majority chromatin is in its extended (decondensed) state
during interphase, only condenses during mitosis.
– Heterochromatin: Remains highly condensed even in interphase. Accounts
for the dark staining regions seen in interphase chromatin. Heterochromatin is
further classified as:
• Constitutive: always inactive and condensed: e.g. repetitive DNA, centromeric
DNA
• Facultative: can exist in both forms. E.g.: Female X chromosome in mammals.
24. 24
• Barr bodies:
– example of heterchromatin decreasing gene activity
• Barr bodies = X Inactivation
• inactivation of one X chromosome to control for dosage compensation in
female mammals
– One X chromosome appears in interphase cells as a darkly stained
heterochromatin mass
– Most of the genes are turned off on the barr body
– Random inactivation of one of the X chromosomes early in development.
– Not the same X in all cells
Epigenetic effects on gene regulation
25. 25
X Inactivation Example
• Calico cats
• Fur color pattern
• Heterozygous for fur color Oo on X chromosomes
– O = orange
– o = black
– White is caused by another gene present in calicos
• Cells where the O allele chromosome is inactivated produce
black pigment
• Cells where the o allele chromosome is inactivated produce
orange pigment
27. 27
How chromosomal packaging influences
gene activity
• Decompaction precedes gene expression
– Boundary elements delimit areas of decompaction
– Nucleosomes in the decompacted area unwind to allow
initiation of transcription
• Transcription factors (nonhistone proteins) unwind nucleosomes
and dislodge histones at 5’ end of genes
• Unwound portion is open to interaction with RNA polymerase
which can recognize promotor and initiate gene expression
30. 30
Epigenetic effects on gene regulation
• Chemical modifications of DNA
• Does NOT change base sequence - NOT a mutation
• Usually methylation of Cytosine in CG sequences
• Example: Extreme condensation silences expression
• Heterochromatin
– Highly compacted even during interphase
– Usually found in regions near centromere
– Constitutive heterochromatin remains condensed most of time in all
cells (e.g., Y chromosomes in flies and humans)
• Remember - Euchromatin
– Contains most genes
– Active regions
33. 33
Transcriptional silencing via methylation:
Blocking transcription factor binding
Transcriptional
activator binds to
unmethylated DNA
This would inhibit the
initiation of transcription
35. 35
• Histone Code is modification of histone tails
by acetylation
• Remember:
– the nucleosome
is an octet of
histone proteins
Epigenetic effects on gene regulation
36. 36
Epigenetic effects on gene regulation
• Histone Acetylation = Gene Activation
– Acetyl groups added to histone tails
• Hyperacetylation = Gene Activation
• Hypoacetylation = Gene Silencing
• Remember:
• DNA methylation = Gene Silencing