Beyond Isometric Twitch: Utilizing lengthening, shortening and isotonic contr...InsideScientific
An important webinar for muscle researchers discussing characterization of complete muscle function by combining lengthening, shortening and isotonic contraction tests with traditional isometric twitch and tetanus measurements.
Quantifying muscle function continues to be an important part of any research where muscle is being directly or indirectly studied. However, a broad survey of literature reveals research remains heavily skewed towards isometric twitch and tetanus testing, which may not necessarily provide the most physiologically relevant data to the researcher. This webinar intends to discuss the subject of complete characterization of muscle and how scientists can combine a number of functional tests in their experimental design to better reveal scientific findings relating to muscle dynamics.
In this webinar, sponsored by Aurora Scientific, experts will show how to increase your experimental toolbox to create protocols that utilize lengthening, shortening and isotonic contractions, in addition to the more common isometric tests. Attendees will also learn when best to implement these new protocols to obtain the most complete data possible.
Three Techniques, One System: How to Effectively Characterize Complete Muscle...InsideScientific
An essential resource for all muscle researchers interested in methodology, best-practices, and techniques for in-vivo, in-vitro, and in-situ muscle experimentation.
As muscle researchers know, functional measurements and assays are the foundation of successful research and are a growing necessity for publication. However, choosing the right experimental technique to answer a broad array of questions can be a challenge.
In this exclusive webinar sponsored by Aurora Scientific, Drs. Christopher Ward and Ramzi Khairallah will discuss methodology, best-practices, and show attendees how to perform basic in-vivo, in-situ, in-vitro experimentation. Discussions will focus on how these techniques can answer questions about animal phenotyping, compound screening, and be used to evaluate interventions and therapies longitudinally. In addition, presenters will review how these techniques can be integrated with other whole animal physiology measurements, biological assays, and genetic tests to provide greater insights into muscle function.
Experimental Muscle Mechanics in Aging and DiseaseInsideScientific
The focus of this webinar is on the mechanisms underlying muscle impairment associated with aging and age-related diseases. This research evaluates the regulation of muscle contraction at the motor protein, single muscle fiber, and whole muscle levels using in vitro, in situ and in vivo methods in experimental and clinical settings. Specifically, Dr. Qaisar is interested in the contribution(s) of muscle SR stress and calcium dysregulation to loss of muscle mass and strength. Reduced calcium uptake by muscle SR plays a pivotal role in muscle impairment in aging while boosting SR calcium uptake with pharmacological activators can partially restore muscle mass and strength. Matt shares a technical overview of the experimental equipment used in this research. He discusses the design, component functionality, and the applications that can be explored with this system. He also shares some valuable insights into the parameters that can be measured and the data that can be produced with various experimental designs.
Key Learning Objectives
- Contractile dysfunction in sarcopenia involves alterations at motor protein, single fibers and whole muscle levels in rodent models of aging and elderly humans
- Loss of calcium regulation by muscle SR plays a pivotal role in muscle atrophy and weakness associated with sarcopenia
- Age-associated comorbidities such as COPD and Parkinson’s disease accelerated the sarcopenia phenotype in humans
- Circulating biomarkers such as CAF22 can be useful predictors of muscle mass and strength in accelerated sarcopenia
Walker E, Sandercock T, Perreault E. Influence of scaling assumptions on tendon stiffness estimation. American Society of Biomechanics 2012, Gainesville FL, August 16 2012. (podium)
Indian Dental Academy: will be one of the most relevant and exciting training center with best faculty and flexible training programs for dental professionals who wish to advance in their dental practice,Offers certified courses in Dental implants,Orthodontics,Endodontics,Cosmetic Dentistry, Prosthetic Dentistry, Periodontics and General Dentistry.
Beyond Isometric Twitch: Utilizing lengthening, shortening and isotonic contr...InsideScientific
An important webinar for muscle researchers discussing characterization of complete muscle function by combining lengthening, shortening and isotonic contraction tests with traditional isometric twitch and tetanus measurements.
Quantifying muscle function continues to be an important part of any research where muscle is being directly or indirectly studied. However, a broad survey of literature reveals research remains heavily skewed towards isometric twitch and tetanus testing, which may not necessarily provide the most physiologically relevant data to the researcher. This webinar intends to discuss the subject of complete characterization of muscle and how scientists can combine a number of functional tests in their experimental design to better reveal scientific findings relating to muscle dynamics.
In this webinar, sponsored by Aurora Scientific, experts will show how to increase your experimental toolbox to create protocols that utilize lengthening, shortening and isotonic contractions, in addition to the more common isometric tests. Attendees will also learn when best to implement these new protocols to obtain the most complete data possible.
Three Techniques, One System: How to Effectively Characterize Complete Muscle...InsideScientific
An essential resource for all muscle researchers interested in methodology, best-practices, and techniques for in-vivo, in-vitro, and in-situ muscle experimentation.
As muscle researchers know, functional measurements and assays are the foundation of successful research and are a growing necessity for publication. However, choosing the right experimental technique to answer a broad array of questions can be a challenge.
In this exclusive webinar sponsored by Aurora Scientific, Drs. Christopher Ward and Ramzi Khairallah will discuss methodology, best-practices, and show attendees how to perform basic in-vivo, in-situ, in-vitro experimentation. Discussions will focus on how these techniques can answer questions about animal phenotyping, compound screening, and be used to evaluate interventions and therapies longitudinally. In addition, presenters will review how these techniques can be integrated with other whole animal physiology measurements, biological assays, and genetic tests to provide greater insights into muscle function.
Experimental Muscle Mechanics in Aging and DiseaseInsideScientific
The focus of this webinar is on the mechanisms underlying muscle impairment associated with aging and age-related diseases. This research evaluates the regulation of muscle contraction at the motor protein, single muscle fiber, and whole muscle levels using in vitro, in situ and in vivo methods in experimental and clinical settings. Specifically, Dr. Qaisar is interested in the contribution(s) of muscle SR stress and calcium dysregulation to loss of muscle mass and strength. Reduced calcium uptake by muscle SR plays a pivotal role in muscle impairment in aging while boosting SR calcium uptake with pharmacological activators can partially restore muscle mass and strength. Matt shares a technical overview of the experimental equipment used in this research. He discusses the design, component functionality, and the applications that can be explored with this system. He also shares some valuable insights into the parameters that can be measured and the data that can be produced with various experimental designs.
Key Learning Objectives
- Contractile dysfunction in sarcopenia involves alterations at motor protein, single fibers and whole muscle levels in rodent models of aging and elderly humans
- Loss of calcium regulation by muscle SR plays a pivotal role in muscle atrophy and weakness associated with sarcopenia
- Age-associated comorbidities such as COPD and Parkinson’s disease accelerated the sarcopenia phenotype in humans
- Circulating biomarkers such as CAF22 can be useful predictors of muscle mass and strength in accelerated sarcopenia
Walker E, Sandercock T, Perreault E. Influence of scaling assumptions on tendon stiffness estimation. American Society of Biomechanics 2012, Gainesville FL, August 16 2012. (podium)
Indian Dental Academy: will be one of the most relevant and exciting training center with best faculty and flexible training programs for dental professionals who wish to advance in their dental practice,Offers certified courses in Dental implants,Orthodontics,Endodontics,Cosmetic Dentistry, Prosthetic Dentistry, Periodontics and General Dentistry.
it is very useful content for the basic knowledge of motor units .
Dedicated to my father shri satyaveer singh , my mother bateri devi and the entire lnipe family .
The Indian Dental Academy is the Leader in continuing dental education , training dentists in all aspects of dentistry and
offering a wide range of dental certified courses in different formats.for more details please visit
www.indiandentalacademy.com
Prehabilitation and Improved Performance for AthletesTina Nguyen
A prehabilitation program to reduce the incidence of anterior cruciate ligament (ACL) injuries. Researched and written as a final project upon gradate school.
Die Ameisen-Roboter BionicAnts sollen in Zukunft die Arbeit in Fabriken erleichtern. Durch Funk-Kommunikation werden selbst schwerste Lasten schnell und koordiniert transportiert.
it is very useful content for the basic knowledge of motor units .
Dedicated to my father shri satyaveer singh , my mother bateri devi and the entire lnipe family .
The Indian Dental Academy is the Leader in continuing dental education , training dentists in all aspects of dentistry and
offering a wide range of dental certified courses in different formats.for more details please visit
www.indiandentalacademy.com
Prehabilitation and Improved Performance for AthletesTina Nguyen
A prehabilitation program to reduce the incidence of anterior cruciate ligament (ACL) injuries. Researched and written as a final project upon gradate school.
Die Ameisen-Roboter BionicAnts sollen in Zukunft die Arbeit in Fabriken erleichtern. Durch Funk-Kommunikation werden selbst schwerste Lasten schnell und koordiniert transportiert.
Project Loon is a research and development project being developed by Google with the mission of providing Internet access to rural and remote areas. The project uses high-altitude balloons placed in the stratosphere at an altitude of about 32 km to create an aerial wireless network with up to 3G-like speeds. Because of the project's seemingly outlandish mission goals, Google named it "Project Loon"
A complete illustrated ppt on 3D printing technology. All the additive processes,Future and effects are well described with relevant diagram and images.Must download for attractive seminar presentation.3D Printing technology could revolutionize and re-shape the world. Advances in 3D printing technology can significantly change and improve the way we manufacture products and produce goods worldwide. If the last industrial revolution brought us mass production and the advent of economies of scale - the digital 3D printing revolution could bring mass manufacturing back a full circle - to an era of mass personalization, and a return to individual craftsmanship.
3D printer Technology _ A complete presentationVijay Patil
Please give a feedback if you like my presentation.
google drive download link :
https://drive.google.com/file/d/1LSLZ-eU8QvihgzJ5BO_sav1im_e0ck0a/view?usp=sharing
Measuring Work in Single Isolated Cardiomyocytes: Replicating the Cardiac CycleInsideScientific
A special webinar for basic cardiovascular researchers interested in a novel technique for measuring work output and replicating the four phases of the cardiac cycle at the single cell level.
The study of isolated cardiac myocytes provide a wealth of basic cellular and molecular information without the complications often associated with heterogeneous multicellular preparations. The overwhelming majority of data presented in myocyte studies, however, are reported in mechanically unloaded conditions. Join us for a practical demonstration of an exciting new technique where mechanical control of the cell reveals the myocyte's force-length relationship by varying pre- and afterload to achieve isometric, isotonic, and, ultimately, work-loop style contractions analogous to the pressure-volume relationship in whole heart studies.
In this exclusive webinar sponsored by IonOptix, Michiel Helmes presents methodology and best-practices that scientists should follow in order to replicate the cardiac cycle in an isolated cardiomyocyte. He discusses how this research method can be used to better address contractile function in cardiovascular disease studies and highlight critical features of the IonOptix MyoStretcher system that are important for this emerging and novel technique.
Electrical Muscle Stimulation for Speed and Strength Development - Derek HansenDerek Hansen
This presentation outlines the evolution of electrical stimulation technology for use in strength and speed development. Globus Sport and Health Technologies has adopted my SpeedCoach protocols to provide the optimal combination of technology and program planning for enhanced performance and recovery for athletes of all sports.
Motor Unit Conduction Velocity During Sustained Contraction Of The Vastus Med...Nosrat hedayatpour
in the current study, we analyzed the
Effect of eccentric exercise on the conduction velocity of
individual motor units at two locations of the vastus
medialis muscle during sustained contractions.
Learn how to implement musculoskeletal laser protocols into your practice. Discover the key clinical breakthroughs in laser therapy and laser protocols. Receive an up-to-date syllabus with graphics and take-home notes. Takeaway information guaranteed to expand your clinical knowledge.
Neuromuscular Adaptations to Sports Training Jill Costley
Neuromuscular Adaptations to Sports Training from my Undergrad Strength and Conditioning placement at the Sports Institute of Northern Ireland. Outcomes: 1) Understand the role of the brain and nervous system in relation to motor control 2) Understand the basic structure of a muscle fibre 3) Appreciate the implications and effects of training on the neuromuscular system. Hope it's useful to someone. Any critical feedback is welcome.
Non-uniform electromyographic activity during fatigue and recovery of the vas...Nosrat hedayatpour
The aim of the study was to investigate EMG signal features
during fatigue and recovery at three locations of the vastus
medialis and lateralis muscles.
Neuromuscular adaptation to exercise –application to practise
CBI Poster_Carissa Miyano- workloop-ALH edits (2)
1. Force produced during eccentric length changes in active mouse soleus and EDL muscles using the
workloop technique
Miyano CA, Felix I, Hessel AL, Dennison BB, Baker EM, Nishikawa KC
Northern Arizona University Center for Bioengineering Innovation
Introduction and Background Objectives Materials and Methods Cont. Results Cont.
The winding filament hypothesis proposes that the protein
N2A titin (red, below) binds to actin (blue) upon Ca2+ influx in
skeletal muscle, and PEVK titin (green) winds on thin
filaments during force development because the cross-
bridges (purple) not only translate but also rotate the thin
filaments.
The workloop technique
• Workloops are used in muscle physiology to test the
mechanical work and power output of muscle contractions
via in vitro muscle testing
• Force and length changes are combined to create a loop,
whose area constitutes the work produced
Results
Acknowledgements
• Muscles move through eccentric (lengthening) and
concentric (shortening) contractions
• Muscle activation during the shortening phase creates
positive work
• Work is done on the environment by the muscle during
shortening
• Application of stimulation during the lengthening phase
creates negative work
• Work is done on the muscle by the environment
Our objectives with the workloop technique
• To characterize eccentric length changes in mouse soleus and
(extensor digitorum longus) EDL muscles
• To compare eccentric length changes in wild-type
heterozygous mice and muscular dystrophy with myositis
(mdm) mice
• Mdm mice carry a deletion in the N2A region of the titin
gene
• Deletion stops titin from binding during activation, possibly
decreasing residual force enhancement during eccentric
contraction
Questions
• Under optimal conditions, what is the work output of mouse
soleus and EDL muscles in healthy wild-type mice?
• Under optimal conditions, what is the work output of mouse
soleus and EDL muscles in mdm mice?
• Will the workloop profile be different between fast twitch and
slow twitch muscles?
• 30 day old mice
• Soleus and EDL muscles were extracted
• Muscles were attached to an isotonic force lever servomotor
(below)
• Muscles remained in oxygenated Krebs solution while
attached to the force lever or while waiting to be used
Mice genotypes
• Wild-type (+,+)
• Heterozygous type (+,-)
• mdm mutant type (-,-)
Workloop trials
1. Optimal muscle length (Lo) was determined
2. Workloops were run using similar parameters for EDL and
soleus muscles
3. Sinusoidal length changes using a constant strain of + or -
5% of Lo while being phasically stimulated produced the
workloops
4. After 5 active trials with 4 full cycles each, passive trials were
conducted to check the integrity of the muscle
5. 5 passive trials were obtained to compare to active trials
6. Muscles were then removed and weighed to normalize forces
to body size
7. Changes in force production and muscle displacement were
controlled, monitored, and analyzed using in-house software
(LabView)
Parameters
EDL
Duration: 1250ms (5 oscillatory cycles of 250ms each)
Amplitude: ±5% Lo
Frequency 1 and 2: 4hz
Pulse rate: 130ms
Train Duration: 40ms
Train offset: 22.5%
Soleus
Duration: 1250ms (5 oscillatory cycle of 250ms each)
Amplitude: ±5% Lo
Frequency 1 and 2: 4hz
Pulse rate: 200ms
Train duration: 40ms
Train offset: 22.5%
EDL workloops
Soleus workloops
Thanks to the Nishikawa labs for helpful feedback. Our funding
sources include NSF IOS-1025806. NSF IOS 0742483 and
NSF IIP 1237878. We would also like to thank the NAU Bio
Annex for animal care.
Materials and Methods
Stationary End
Lever
Parallel Electrodes
Comparison of negative work between Wild-Type
(healthy) EDL and soleus
Comparison of negative work between genotypes of EDL
and soleus
Conclusions and Future Work
• Total mechanical work absorbed and the rate of force
development were attenuated in mdm mice compared to wild-
type mice
• The workloop profile for wild-type mice was substantially
different between fast and slow twitch muscle in a way that
maximized muscle performance and function
• The results suggest that the deletion in the N2A region of titin
leads to a decrease in negative work during repetitive
movements, however it does not change muscle
activation/deactivation mechanisms.
Future work
• Continued data collection
• Further studies will use more realistic workloop parameters
that focus on physiologically relevant length changes and
stimulation frequencies