Cardiovascular Research Focus by Proteintech
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Proteintech offers a wide range of markers and related antibodies for cardiovascular research. Slideshow includes: Age-associated Changes in Cardiovascular Tissues mTOR in Cardiac Physiology and Disease Cardiac Troponin T Antibody Cardio-related Antibodies
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Basics of Immunohistochemistry (IHC)
Immunohistochemistry (IHC) is a highly sensitive method that allows the localization of antigen within a cell or a tissue with high resolution. The method is based on the use of a primary antibody that specifically binds to its complementary antigen. The bound antibody may then be visualized by a variety of methods such as colorimetric end points.
Presentation of Frank Hills in 1st International Antibody Validation Forum 2014
Presentation of Frank Hills in 1st International Antibody Validation Forum 2014St John's Laboratory Ltd
After graduating with an honours degree in Biochemistry Dr Hills worked for several years as a Clinical Scientist at St Bartholomew's hospital in London. He was awarded his PhD in 2002 from the faculty of Medicine at Queen Mary University of London. He continued his interest in reproductive science at Imperial College London where he worked as a postdoctoral researcher before joining Middlesex in 2004 as a lecturer. Dr Hills has published many high profile original research articles on various aspects of obstetric pathology including pre-eclampsia, recurrent miscarriage, preterm labour and fetal distress as well as several articles in the area of assisted reproduction. Currently, he is research interests include investigating the role of glycosaminoglycans and proteoglycans on the development of placental pathology and breast cancer. Dr Hills teaches a range of topics in biomedical science including clinical biochemistry, cellular and developmental biology as well as statistical analysis. He is author of around 30 peer-reviewed scientific articles and has refereed manuscripts for a variety of journals in the area of reproduction and endocrinology.
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This method is used to visualise the localisation and quantity of a protein of interest. The target protein is bound to by a specific primary antibody, which in turn is detected by a secondary antibody conjugated to a fluorophore. A fluorescent or confocal microscope is used to visualise the protein.
Immunocytochemistry (ICC) differs from immunohistochemistry (IHC) in that the former is performed on samples of intact cells that have had most, if not all, of their surrounding extracellular matrix removed. In contrast, immunohistochemical samples are sections of biological tissue, where each cell is surrounded by tissue architecture and other cells normally found in the intact tissue. These differences cause the samples to be prepared differently. For ICC, the sample requires permeabilisation so that the antibodies can reach the intracellular targets. Depending on the thickness of the sample, IHC samples do not require this.
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This document provides information on immunohistochemistry (IHC), including:
1. IHC is used to detect antigens in tissues through antigen-antibody recognition at the light microscopic level. It applies immunologic principles and techniques to study cells and tissues.
2. The basic principle of IHC is a sharp visualization of target components in cells and tissues based on a satisfactory signal-to-noise ratio.
3. The main steps of IHC are tissue processing, antigen retrieval, primary/secondary antibody incubation, detection, counterstaining, and mounting. Proper controls and interpretation of results are also discussed.
Basics of Immunohistochemistry
Important auxiliary method for pathologists in routine diagnostic work as well as in basic and clinical research including exploration of biomarkers, as IHC allows confirmation of target molecule expressions in the context of microenvironment.
pathogenesis, diagnosis, and treatment of diseases are discovered.It is performed without destruction of histologic architecture. It can be used as an effective adjuvant to H & E diagnosis in a majority of tumor cases through the establishment of definitive diagnosis and also gives an insight into tumor histopathogenesis and prognosis.
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The document describes the process for producing monoclonal antibodies. Mice are immunized with an antigen to elicit an immune response. Spleen cells from mice with high antibody titers are then fused with myeloma cells to generate immortal hybridoma cells. These hybridoma cells are screened and those that secrete the desired antibodies are isolated and mass cultured to produce monoclonal antibodies specific to the target antigen.
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Monoclonal antibodies
Monoclonal antibodies are antibodies that are directed against a specific antigen. They are produced by a single clone of cells and can be obtained from immortalized B-lymphocytes or recombinant cell lines. The production of monoclonal antibodies involves immunizing an animal, fusing its spleen cells with myeloma cells to form hybridomas, selecting hybridomas that secrete the desired antibody, and propagating these cells to produce large amounts of monoclonal antibodies. Monoclonal antibodies have various applications in cancer therapy, organ transplantation, disease diagnosis, and protein purification. Recent developments include FDA approvals of monoclonal antibodies for treating cancer and blood disorders.
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This document summarizes immunohistochemistry (IHC) techniques. IHC combines immunological and histological methods to identify specific proteins in tissues using antigen-antibody reactions. The document discusses common IHC methods like direct, indirect, and enzyme-linked methods. It also covers important considerations for IHC like antibody selection, tissue fixation and processing, antigen retrieval, blocking, and controls. The goal of IHC is to visualize the distribution and localization of cellular components, but the quality of results depends highly on proper sample preparation.
Avacta Life Sciences Affimers Presentation Global Protein Engineering Summit ...
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You can read more about Affimer technology here http://www.avactalifesciences.com
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In addition, several companies were interested in the use of Affimers as an alternative to antibodies in diagnostic devices, mainly because they could generate binders against new biomarkers much more quickly and evaluate them in higher numbers.
The benefits of Affimer microarrays for biomarker discovery also resonated with diagnostic developers who appreciated the advantage of being able to evaluate significantly larger numbers of potential biomarkers more cost and time effectively than by mass spectrometry. The potential of the arrays for multiplexed solutions for clinical diagnosis and monitoring during drug trials was also something that generated interest amongst those delegates.
Matt Johnson, Chief Technical Officer of Avacta Life Sciences commented: "It was great to experience face to face the level of interest in Affimers. The majority of people I spoke to were either having problems raising antibodies to their target of interest or just couldn't use antibodies because of the type of assays they wanted to perform. Many of the presentations focused around the use of antibody fragments for intra-cellular studies which is a rapidly growing area that holds great interest for drug and diagnostics developers. It is an area where there are clear advantages for Affimers over antibody fragments which don't behave well in the cytoplasm.
"The general enthusiasm around Affimers was very encouraging and the amount of interest generated by the potential of Affimers as therapeutics and by the Affimer arrays for biomarker discovery only reinforces my excitement around this new technology."
Monoclonal Antibodies
This document discusses monoclonal antibodies (mAbs), including their definition, production process, structure, applications, and limitations. It describes how mAbs are produced through the fusion of immune spleen cells from immunized mice with myeloma cells, which results in hybridoma cells that can produce identical antibodies. The key steps involved are immunizing mice, screening for antibody production, preparing myeloma cells, fusing cells, cloning hybridomas, and selecting cells in HAT medium. mAbs have various analytical, preparative, and therapeutic applications, but also have limitations such as immunogenicity and production challenges.
Human monoclonal antibody development
The document describes a proprietary method from Bioworld Consulting Laboratories for generating human antibodies. It captures and immortalizes natural human antibody producing cells to produce antigen-specific human antibodies in vitro. This avoids losing antigen-specific B-cells associated with other methods. It produces natural human antibodies characterized without recombinant technology. Within 6-8 weeks it can obtain monoclonal antibody producing clones, compared to years for traditional methods.
Immunohistochemistry in pathology laboratory
Immunohistochemistry (IHC) utilizes labeled antibodies to localize specific antigens in tissue sections through antigen-antibody interactions visualized by markers like fluorescent dyes or enzymes. IHC allows visualization of the distribution and localization of cellular components within tissues. The process involves raising antibodies to target antigens, labeling the antibodies, and applying them to tissue sections using techniques like direct, indirect, or peroxidase anti-peroxidase (PAP) methods. IHC is a sensitive and specific technique that is useful for cancer diagnosis and research applications.
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AriMandlerPoster1
1) The document examines ATF3 expression in cardiomyocytes in response to phenylephrine (PE) treatment.
2) Immunofluorescent staining of cardiomyocytes showed increased ATF3 nuclear staining after 0.5 hours of PE treatment, which decreased after 2 hours.
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Recombinant therapeutic proteins
This document discusses the production of recombinant therapeutic proteins. It provides a timeline of important developments including the approval of the first recombinant human insulin in 1982. It outlines the typical process of drug production including transfection of cells, cell culture, purification and formulation. It discusses the advantages and disadvantages of expressing proteins in bacterial cells, yeast cells, insect cells, plants and transgenic animals. Finally, it provides examples of some important recombinant proteins that have been approved for human use to treat disorders like hemophilia and diabetes.
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This document provides guidance on optimizing immunofluorescence staining techniques. It discusses fundamental principles, sample preparation including fixation, controls, troubleshooting tips, and visualization methods. The key steps covered are fixation to preserve cell structure while maintaining epitopes, controlling for autofluorescence, choosing direct or indirect visualization depending on the target antigen expression level, and selecting mounting media based on the specific application. Proper controls and troubleshooting recommendations are also outlined to ensure specific and high quality staining results.
Immunohistochemistry
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.
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Basics of Immunohistochemistry (IHC)
Immunohistochemistry (IHC) is a highly sensitive method that allows the localization of antigen within a cell or a tissue with high resolution. The method is based on the use of a primary antibody that specifically binds to its complementary antigen. The bound antibody may then be visualized by a variety of methods such as colorimetric end points.
Presentation of Frank Hills in 1st International Antibody Validation Forum 2014
Presentation of Frank Hills in 1st International Antibody Validation Forum 2014St John's Laboratory Ltd
After graduating with an honours degree in Biochemistry Dr Hills worked for several years as a Clinical Scientist at St Bartholomew's hospital in London. He was awarded his PhD in 2002 from the faculty of Medicine at Queen Mary University of London. He continued his interest in reproductive science at Imperial College London where he worked as a postdoctoral researcher before joining Middlesex in 2004 as a lecturer. Dr Hills has published many high profile original research articles on various aspects of obstetric pathology including pre-eclampsia, recurrent miscarriage, preterm labour and fetal distress as well as several articles in the area of assisted reproduction. Currently, he is research interests include investigating the role of glycosaminoglycans and proteoglycans on the development of placental pathology and breast cancer. Dr Hills teaches a range of topics in biomedical science including clinical biochemistry, cellular and developmental biology as well as statistical analysis. He is author of around 30 peer-reviewed scientific articles and has refereed manuscripts for a variety of journals in the area of reproduction and endocrinology.
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This document provides information about monoclonal antibodies including their production, types, and applications. It discusses how monoclonal antibodies are produced using the hybridoma technique which fuses antibody producing B cells with myeloma cells. This results in immortal cell lines that produce identical antibodies targeting a single epitope. The document contrasts monoclonal and polyclonal antibodies and describes the evolution of monoclonal antibodies from murine to humanized and human forms to reduce immunogenicity. It also covers monoclonal antibody nomenclature, pharmacokinetics, adverse effects, and therapeutic uses.
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This document provides an overview of immunohistochemistry (IHC), including its history, basic methods, considerations, and applications. IHC combines histology, immunology, and biochemical techniques to visualize the distribution and localization of specific cellular components within tissues through antigen-antibody reactions tagged with visible labels. Key steps discussed include fixation, sectioning, antigen retrieval, blocking, controls, direct and indirect antibody binding methods, detection methods including enzymatic and fluorescence techniques, and important considerations for optimizing IHC experiments.
Monoclonal antibody and its delivery
This document discusses monoclonal antibodies, including their structure, types, methods of preparation, applications, and marketed products. Monoclonal antibodies are homogeneous antibodies produced by identical immune cells that are clones of a single parent cell. They are prepared using the hybridoma technology which involves immunizing an animal, fusing immune cells with myeloma cells to form hybridomas, screening and selecting hybridomas that produce the desired monoclonal antibody. Monoclonal antibodies have various therapeutic applications in areas such as cancer, autoimmune diseases, organ transplants, and infections. They are also used for diagnostic purposes.
Immunocytochemistry Protocol
This method is used to visualise the localisation and quantity of a protein of interest. The target protein is bound to by a specific primary antibody, which in turn is detected by a secondary antibody conjugated to a fluorophore. A fluorescent or confocal microscope is used to visualise the protein.
Immunocytochemistry (ICC) differs from immunohistochemistry (IHC) in that the former is performed on samples of intact cells that have had most, if not all, of their surrounding extracellular matrix removed. In contrast, immunohistochemical samples are sections of biological tissue, where each cell is surrounded by tissue architecture and other cells normally found in the intact tissue. These differences cause the samples to be prepared differently. For ICC, the sample requires permeabilisation so that the antibodies can reach the intracellular targets. Depending on the thickness of the sample, IHC samples do not require this.
Do you have a technical question? Get in touch: info@stjohnslabs.com
Immunohistochemistry
This document provides information on immunohistochemistry (IHC), including:
1. IHC is used to detect antigens in tissues through antigen-antibody recognition at the light microscopic level. It applies immunologic principles and techniques to study cells and tissues.
2. The basic principle of IHC is a sharp visualization of target components in cells and tissues based on a satisfactory signal-to-noise ratio.
3. The main steps of IHC are tissue processing, antigen retrieval, primary/secondary antibody incubation, detection, counterstaining, and mounting. Proper controls and interpretation of results are also discussed.
Basics of Immunohistochemistry
Important auxiliary method for pathologists in routine diagnostic work as well as in basic and clinical research including exploration of biomarkers, as IHC allows confirmation of target molecule expressions in the context of microenvironment.
pathogenesis, diagnosis, and treatment of diseases are discovered.It is performed without destruction of histologic architecture. It can be used as an effective adjuvant to H & E diagnosis in a majority of tumor cases through the establishment of definitive diagnosis and also gives an insight into tumor histopathogenesis and prognosis.
Production of Monoclonal Antibodies and its application : Rituximab
The document describes the process for producing monoclonal antibodies. Mice are immunized with an antigen to elicit an immune response. Spleen cells from mice with high antibody titers are then fused with myeloma cells to generate immortal hybridoma cells. These hybridoma cells are screened and those that secrete the desired antibodies are isolated and mass cultured to produce monoclonal antibodies specific to the target antigen.
Søren nielsen ihc pitfalls - leica 2011
The document discusses common pitfalls in diagnostic immunohistochemistry (IHC), including issues that can occur in pre-analytic, analytic, and post-analytic phases. Some key pitfalls mentioned are insufficient tissue fixation time, improper epitope retrieval methods, suboptimal antibody choices, and lack of appropriate controls. The author emphasizes the importance of validation, controls, and following standardized protocols to avoid false positives and false negatives in IHC.
Monoclonal antibodies
Monoclonal antibodies are antibodies that are directed against a specific antigen. They are produced by a single clone of cells and can be obtained from immortalized B-lymphocytes or recombinant cell lines. The production of monoclonal antibodies involves immunizing an animal, fusing its spleen cells with myeloma cells to form hybridomas, selecting hybridomas that secrete the desired antibody, and propagating these cells to produce large amounts of monoclonal antibodies. Monoclonal antibodies have various applications in cancer therapy, organ transplantation, disease diagnosis, and protein purification. Recent developments include FDA approvals of monoclonal antibodies for treating cancer and blood disorders.
Immunohistochemistry
This document summarizes immunohistochemistry (IHC) techniques. IHC combines immunological and histological methods to identify specific proteins in tissues using antigen-antibody reactions. The document discusses common IHC methods like direct, indirect, and enzyme-linked methods. It also covers important considerations for IHC like antibody selection, tissue fixation and processing, antigen retrieval, blocking, and controls. The goal of IHC is to visualize the distribution and localization of cellular components, but the quality of results depends highly on proper sample preparation.
Avacta Life Sciences Affimers Presentation Global Protein Engineering Summit ...
Avacta Life Sciences Exhibits Affimers at Global Protein Engineering Summit
Avacta Life Sciences exhibited recently at the Global Protein Engineering Summit ("PEGS") where it presented its Affimer technology.
You can read more about Affimer technology here http://www.avactalifesciences.com
PEGS is considered to be the essential protein engineering meeting where commercial and academic progress in protein engineering is showcased and this year it attracted over 1800 delegates from across the globe to Boston. Avacta Life Sciences presented its Affimer technology for the first time at a PEGS meeting with technical exhibits and a presentation by the CSO, Paul Ko Ferrigno, entitled "Biological Recognition: Beyond the Antibody."*
The exhibition booth was busy with over 80 delegates talking to the Avacta Life Science management team over the four days of the summit. The feedback on the Affimer technology was very positive, in particular, the short development times and excellent stability were highlighted by delegates as key advantages of Affimers over antibodies. There was also a strong interest in Affimers from the management of companies developing biological therapeutics who were keen to learn more about the potential of Affimers as novel therapeutics.
In addition, several companies were interested in the use of Affimers as an alternative to antibodies in diagnostic devices, mainly because they could generate binders against new biomarkers much more quickly and evaluate them in higher numbers.
The benefits of Affimer microarrays for biomarker discovery also resonated with diagnostic developers who appreciated the advantage of being able to evaluate significantly larger numbers of potential biomarkers more cost and time effectively than by mass spectrometry. The potential of the arrays for multiplexed solutions for clinical diagnosis and monitoring during drug trials was also something that generated interest amongst those delegates.
Matt Johnson, Chief Technical Officer of Avacta Life Sciences commented: "It was great to experience face to face the level of interest in Affimers. The majority of people I spoke to were either having problems raising antibodies to their target of interest or just couldn't use antibodies because of the type of assays they wanted to perform. Many of the presentations focused around the use of antibody fragments for intra-cellular studies which is a rapidly growing area that holds great interest for drug and diagnostics developers. It is an area where there are clear advantages for Affimers over antibody fragments which don't behave well in the cytoplasm.
"The general enthusiasm around Affimers was very encouraging and the amount of interest generated by the potential of Affimers as therapeutics and by the Affimer arrays for biomarker discovery only reinforces my excitement around this new technology."
Monoclonal Antibodies
This document discusses monoclonal antibodies (mAbs), including their definition, production process, structure, applications, and limitations. It describes how mAbs are produced through the fusion of immune spleen cells from immunized mice with myeloma cells, which results in hybridoma cells that can produce identical antibodies. The key steps involved are immunizing mice, screening for antibody production, preparing myeloma cells, fusing cells, cloning hybridomas, and selecting cells in HAT medium. mAbs have various analytical, preparative, and therapeutic applications, but also have limitations such as immunogenicity and production challenges.
Human monoclonal antibody development
The document describes a proprietary method from Bioworld Consulting Laboratories for generating human antibodies. It captures and immortalizes natural human antibody producing cells to produce antigen-specific human antibodies in vitro. This avoids losing antigen-specific B-cells associated with other methods. It produces natural human antibodies characterized without recombinant technology. Within 6-8 weeks it can obtain monoclonal antibody producing clones, compared to years for traditional methods.
Immunohistochemistry in pathology laboratory
Immunohistochemistry (IHC) utilizes labeled antibodies to localize specific antigens in tissue sections through antigen-antibody interactions visualized by markers like fluorescent dyes or enzymes. IHC allows visualization of the distribution and localization of cellular components within tissues. The process involves raising antibodies to target antigens, labeling the antibodies, and applying them to tissue sections using techniques like direct, indirect, or peroxidase anti-peroxidase (PAP) methods. IHC is a sensitive and specific technique that is useful for cancer diagnosis and research applications.
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Presentation of Frank Hills in 1st International Antibody Validation Forum 2014
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Seminar 112108
The document summarizes research using redox ICAT and mass spectrometry techniques to identify 102 proteins in mouse heart tissue that are targets of reduction by thioredoxin 1 (Trx1) under conditions of cardiac stress and hypertrophy. The identified Trx1 reduction targets are involved in metabolic pathways like glycolysis, the TCA cycle, and beta-oxidation, as well as the electron transport chain, mitochondrial permeability transition pore, and myofibrils. The Trx1 reduction network appears to facilitate ATP production and utilization, contributing to Trx1's anti-hypertrophic functions.
AriMandlerPoster1
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2) Immunofluorescent staining of cardiomyocytes showed increased ATF3 nuclear staining after 0.5 hours of PE treatment, which decreased after 2 hours.
3) Quantitative PCR analysis found c-Jun mRNA was induced after 2 hours of PE, but ATF3, Egr1, and Fos mRNA levels remained unchanged.
Mb cardiovascular
The document discusses cardiovascular biomarkers and tools for discovering and detecting markers of cardiac disease. It summarizes Enzo Life Sciences' portfolio of products including immunoassays, antibodies, activity assays, and small molecule libraries for analyzing cardiac function, inflammation, vascular biology, and more. The tools can be used to study conditions like heart failure, blood clotting disorders, and cardiovascular diseases. Enzo provides over 300 immunoassay kits, 3000 antibodies and activity assays to enable research on signaling pathways, apoptosis, vascular regulation and other processes relevant to cardiac health and disease.
Mechanism of Rhein Inhibiting Acute Myocardial Infarction–Induced Endoplasmic...
Mechanism of Rhein Inhibiting Acute Myocardial Infarction–Induced Endoplasmic...ANALYTICAL AND QUANTITATIVE CYTOPATHOLOGY AND HISTOPATHOLOGY
This study explored whether the compound Rhein can inhibit stress in the endoplasmic reticulum and mitochondria caused by acute myocardial infarction through activating the PI3K/Akt/ERK signaling pathway. A rat model of myocardial infarction was used. Rats treated with Rhein showed improved cardiac function and reduced cardiomyocyte apoptosis compared to untreated rats. Rhein was found to inhibit expression of endoplasmic reticulum and mitochondrial proteins associated with stress and apoptosis, while elevating expression of proteins in the PI3K/Akt/ERK pathway. The results suggest Rhein can protect against myocardial damage from infarction by reducing endoplasmic reticulum and mitochondrial dysfunction through this signaling pathway.Mb cell death-apoptosis
This document discusses apoptosis analysis tools from Enzo Life Sciences. It describes their products for monitoring the intrinsic and extrinsic apoptosis pathways from the cell membrane to the nucleus. Their product lines include modulators and screening assays, immunoassays and antibodies, and live cell analysis tools for detecting hallmarks of apoptosis like phosphatidylserine exposure, chromatin condensation, DNA damage, and caspase activation. The document provides examples of specific products for analyzing apoptotic pathways.
Oxidative stress and atrial fibrillation: a long journey in to the clinic? (P...
Oxidative stress and atrial fibrillation: a long journey in to the clinic? (P...Vall d'Hebron Institute of Research (VHIR)
This document summarizes research on the role of oxidative stress and the enzyme NADPH oxidase 2 (NOX2) in atrial fibrillation (AF). The key points are:
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2. Experiments in animal models show that increased atrial NOX2 activity, through genetic overexpression of NOX2, is sufficient to create a substrate for AF induction.
3. Pharmacological inhibition of NOX2, such as with statins, prevents AF in animal models and reduces the risk of postoperative AFGraduate Seminar - Halloween 2009
- The document discusses neuronal cell death in Alzheimer's disease, which is linked to two protein aggregates - amyloid beta plaques and tau tangles. When amyloid beta is introduced to healthy mouse neurons in culture, it leads to rapid cell death.
- Amyloid beta treatment of the neurons increases the activity of calcium-dependent proteases (calpains) and cysteine proteases (caspases), which quickly degrade tau through cleavage into lower molecular weight fragments. This degradation of tau by proteases appears to contribute to neuronal cell death.
- The phosphorylation of tau by kinases such as GSK3 beta and CDK5 also increases after amyloid beta treatment, further implicating tau changes in the neuronal death caused by amyloid
Nobels days 2013
1) The document examines how 17β-estradiol may reduce vascular inflammation by down-regulating the NLRP3 inflammasome and related proinflammatory cytokines like IL-6 and IL-8 in human umbilical vein endothelial cells (HUVECs) and aortic smooth muscle cells (AOSMCs).
2) Exogenous 17β-estradiol was shown to significantly decrease expression of NLRP3, caspase-1, and IL-1β in HUVECs and also reduced IL-1β, IL-6, and IL-8 levels in AOSMCs.
3) Down-regulation of the NLRP3 inflammasome and proinflammatory cytokines by estrogen may be one mechanism for
101111 als
Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease that causes progressive weakness due to the loss of motor neurons in the brain and spinal cord. The cause of ALS is largely unknown, though some genetic and environmental factors have been associated with increased risk. While currently there is no cure for ALS, treatments aim to slow progression and manage symptoms such as spasticity, pain, and respiratory failure through medications, nutrition support, and respiratory assistance.
Axonal Polyneuropathy with Anti-Caspr1 Igg1 Paranodal Antibodies
The main electrophysiological manifestation of
autoimmune paranodopathy with anti-contactin-associated protein
1 (Caspr1) positive reported previously was demyelination, with
or without axonal involvement. Here, we reported a patient with
anti-Caspr1 IgG1 positive who had severe axonal involvement accompanied less evidence of demyelination.
Cardiomyocyte inflammation and SNRK
Cardiac inflammation plays a major role during myocardial infarction, reduction of inflammation decreases the severity of disease progression. SNRK is a AMPK family member protein which is highly involved in decreasing the inflammation in heart and protects cardiac function.
Liver ischemia/reperfusion injury, a setting in which the functional mass is ...
Liver ischemia/reperfusion injury, a setting in which the functional mass is ...Prof. Hesham N. Mustafa
The document discusses a study on the effects of the phosphodiesterase type-5 (PDE5) inhibitor tadalafil on liver ischemia/reperfusion injury in rats. The study found that tadalafil treatment before ischemia/reperfusion injury helped restore normal liver enzyme levels, reduced oxidative stress and inflammation in liver tissue, and decreased levels of tumor necrosis factor-alpha, interleukin-6, and intercellular adhesion molecule-1. Histological analysis also showed tadalafil treatment helped protect against liver damage. The findings suggest that modulating the inflammatory response may be one mechanism by which tadalafil provides hepatoprotection against ischemia/reperfusion injury.Examining Drug Candidates for Pulmonary Arterial Hypertension: The Ups and Do...
Originally presented at the 2014 Safety Pharmacology Society Annual Meeting, this presentation offers a clear understanding of the differences as well as the benefits of monocrotaline, hypoxia with and without VEGF inhibition and immunodeficiency as preclinical initiators of PAH. A past SPS president, Dr. Gralinski will also provided insight on how your compounds may prevent or reverse PAH on the path to the clinic.
BBRC pcsk9
This study investigated the expression and function of PCSK9 in pancreatic islets. The key findings were:
1) PCSK9 was expressed specifically in somatostatin-producing delta cells in human pancreatic islets, but not in alpha or beta cells.
2) PCSK9 deficiency led to increased LDLR protein levels in mouse pancreatic islets, mainly in beta cells. However, PCSK9 deficiency did not alter cholesterol content or insulin secretion from mouse islets.
3) In vivo studies in mice found that glucose tolerance and insulin secretion were similar between PCSK9 deficient and normal mice, suggesting PCSK9 does not impact insulin secretion.
Alpha Anti-Trypsin
Introduction
Structure of the protein
Hubs network with other's protein
Function
Incase of Deficiency
Case study
Conclusion
References
Human, Eukaryotic And Vitro Associations Of Murine Sec...
The document describes experiments to study the effects of vitamin D receptor (VDR) binding to vitamin D response elements (VDREs). Reporter plasmids containing wildtype or mutant VDREs upstream of firefly luciferase were constructed. These plasmids were transfected into HEK293T cells to assess VDR binding and activation of luciferase expression in response to vitamin D treatment.
Research Lapchak
The document summarizes a research study that tested the effects of combining a von Willebrand factor neutralizing antibody (AJW200) with low-dose tissue plasminogen activator (tPA) treatment after embolic stroke in rabbits. The study found that the combination of AJW200 and low-dose tPA significantly improved behavioral outcomes compared to low-dose tPA or IgG control, and produced similar effects to standard-dose tPA alone. This suggests that neutralizing von Willebrand factor may help suppress acute stroke responses like inflammation and platelet aggregation, and could allow the use of lower, safer tPA doses for stroke treatment.
Marcos Malumbres - Centro Nacional de Investigaciones Oncológica (CNIO).
El miércoles 27 de septiembre del 2017 organizamos en la Fundación Ramón Areces un Simposio Internacional sobre nuevas perspectivas en la investigación sobre el cáncer. En colaboración con el Centro Nacional de Investigaciones Oncológicas (CNIO) y Weizmann Institute for Science.
Tpa aptamer
This document describes a study that used SELEX (Systematic Evolution of Ligands by EXponential enrichment) to isolate RNA aptamers that bind to tissue-type plasminogen activator (tPA) and inhibit its interaction with low-density lipoprotein receptor family members, specifically low density lipoprotein receptor-related protein-1 (LRP-1). Two aptamers, K18 and K32, were found to efficiently inhibit the association between tPA and LRP-1 without interfering with tPA's fibrinolytic activity. These aptamers may improve the safety of thrombolytic treatment for cerebral ischemic stroke by restricting tPA's activity to the blood vessel lumen.
DICLE poster JWT edits
ABCC10 loss enhances the effectiveness of chemotherapy drugs like docetaxel in lung cancer cells. Knocking down ABCC10 increased apoptosis and decreased tumor growth in response to docetaxel treatment. ABCC10 loss also reduced activation of signaling proteins involved in cell growth like EGFR, AKT and SRC. This suggests ABCC10 promotes chemoresistance and tumor growth by regulating these oncogenic pathways.
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Mechanism of Rhein Inhibiting Acute Myocardial Infarction–Induced Endoplasmic...
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Oxidative stress and atrial fibrillation: a long journey in to the clinic? (P...
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Axonal Polyneuropathy with Anti-Caspr1 Igg1 Paranodal Antibodies
Axonal Polyneuropathy with Anti-Caspr1 Igg1 Paranodal Antibodies
Liver ischemia/reperfusion injury, a setting in which the functional mass is ...
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Examining Drug Candidates for Pulmonary Arterial Hypertension: The Ups and Do...
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Human, Eukaryotic And Vitro Associations Of Murine Sec...
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Marcos Malumbres - Centro Nacional de Investigaciones Oncológica (CNIO).
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This document provides information on an immunoprecipitation and chromatin immunoprecipitation workshop. It discusses the differences between IP, Co-IP, ChIP, and RIP techniques. It also outlines considerations for performing IP and ChIP experiments such as antibody choice, sample preparation, crosslinking, sonication, washing buffers, and troubleshooting tips. The workshop covers both theoretical background and practical protocols for IP and ChIP.
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Unlocking the mysteries of reproduction: Exploring fecundity and gonadosomati...
The pygmy halfbeak Dermogenys colletei, is known for its viviparous nature, this presents an intriguing case of relatively low fecundity, raising questions about potential compensatory reproductive strategies employed by this species. Our study delves into the examination of fecundity and the Gonadosomatic Index (GSI) in the Pygmy Halfbeak, D. colletei (Meisner, 2001), an intriguing viviparous fish indigenous to Sarawak, Borneo. We hypothesize that the Pygmy halfbeak, D. colletei, may exhibit unique reproductive adaptations to offset its low fecundity, thus enhancing its survival and fitness. To address this, we conducted a comprehensive study utilizing 28 mature female specimens of D. colletei, carefully measuring fecundity and GSI to shed light on the reproductive adaptations of this species. Our findings reveal that D. colletei indeed exhibits low fecundity, with a mean of 16.76 ± 2.01, and a mean GSI of 12.83 ± 1.27, providing crucial insights into the reproductive mechanisms at play in this species. These results underscore the existence of unique reproductive strategies in D. colletei, enabling its adaptation and persistence in Borneo's diverse aquatic ecosystems, and call for further ecological research to elucidate these mechanisms. This study lends to a better understanding of viviparous fish in Borneo and contributes to the broader field of aquatic ecology, enhancing our knowledge of species adaptations to unique ecological challenges.
Nucleic Acid-its structural and functional complexity.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
ESR spectroscopy in liquid food and beverages.pptx
With increasing population, people need to rely on packaged food stuffs. Packaging of food materials requires the preservation of food. There are various methods for the treatment of food to preserve them and irradiation treatment of food is one of them. It is the most common and the most harmless method for the food preservation as it does not alter the necessary micronutrients of food materials. Although irradiated food doesn’t cause any harm to the human health but still the quality assessment of food is required to provide consumers with necessary information about the food. ESR spectroscopy is the most sophisticated way to investigate the quality of the food and the free radicals induced during the processing of the food. ESR spin trapping technique is useful for the detection of highly unstable radicals in the food. The antioxidant capability of liquid food and beverages in mainly performed by spin trapping technique.
THEMATIC APPERCEPTION TEST(TAT) cognitive abilities, creativity, and critic...
THEMATIC APPERCEPTION TEST(TAT) cognitive abilities, creativity, and critic...Abdul Wali Khan University Mardan,kP,Pakistan
hematic appreciation test is a psychological assessment tool used to measure an individual's appreciation and understanding of specific themes or topics. This test helps to evaluate an individual's ability to connect different ideas and concepts within a given theme, as well as their overall comprehension and interpretation skills. The results of the test can provide valuable insights into an individual's cognitive abilities, creativity, and critical thinking skillsEquivariant neural networks and representation theory
Or: Beyond linear.
Abstract: Equivariant neural networks are neural networks that incorporate symmetries. The nonlinear activation functions in these networks result in interesting nonlinear equivariant maps between simple representations, and motivate the key player of this talk: piecewise linear representation theory.
Disclaimer: No one is perfect, so please mind that there might be mistakes and typos.
dtubbenhauer@gmail.com
Corrected slides: dtubbenhauer.com/talks.html
bordetella pertussis.................................ppt
Bordettela is a gram negative cocobacilli spread by air born drop let
如何办理(uvic毕业证书)维多利亚大学毕业证本科学位证书原版一模一样
原版纸张【微信：741003700 】【(uvic毕业证书)维多利亚大学毕业证】【微信：741003700 】学位证，留信认证（真实可查，永久存档）offer、雅思、外壳等材料/诚信可靠,可直接看成品样本，帮您解决无法毕业带来的各种难题！外壳，原版制作，诚信可靠，可直接看成品样本。行业标杆！精益求精，诚心合作，真诚制作！多年品质 ,按需精细制作，24小时接单,全套进口原装设备。十五年致力于帮助留学生解决难题，包您满意。
本公司拥有海外各大学样板无数，能完美还原海外各大学 Bachelor Diploma degree, Master Degree Diploma
1:1完美还原海外各大学毕业材料上的工艺：水印，阴影底纹，钢印LOGO烫金烫银，LOGO烫金烫银复合重叠。文字图案浮雕、激光镭射、紫外荧光、温感、复印防伪等防伪工艺。材料咨询办理、认证咨询办理请加学历顾问Q/微741003700
留信网认证的作用:
1:该专业认证可证明留学生真实身份
2:同时对留学生所学专业登记给予评定
3:国家专业人才认证中心颁发入库证书
4:这个认证书并且可以归档倒地方
5:凡事获得留信网入网的信息将会逐步更新到个人身份内，将在公安局网内查询个人身份证信息后，同步读取人才网入库信息
6:个人职称评审加20分
7:个人信誉贷款加10分
8:在国家人才网主办的国家网络招聘大会中纳入资料，供国家高端企业选择人才
Phenomics assisted breeding in crop improvement
As the population is increasing and will reach about 9 billion upto 2050. Also due to climate change, it is difficult to meet the food requirement of such a large population. Facing the challenges presented by resource shortages, climate
change, and increasing global population, crop yield and quality need to be improved in a sustainable way over the coming decades. Genetic improvement by breeding is the best way to increase crop productivity. With the rapid progression of functional
genomics, an increasing number of crop genomes have been sequenced and dozens of genes influencing key agronomic traits have been identified. However, current genome sequence information has not been adequately exploited for understanding
the complex characteristics of multiple gene, owing to a lack of crop phenotypic data. Efficient, automatic, and accurate technologies and platforms that can capture phenotypic data that can
be linked to genomics information for crop improvement at all growth stages have become as important as genotyping. Thus,
high-throughput phenotyping has become the major bottleneck restricting crop breeding. Plant phenomics has been defined as the high-throughput, accurate acquisition and analysis of multi-dimensional phenotypes
during crop growing stages at the organism level, including the cell, tissue, organ, individual plant, plot, and field levels. With the rapid development of novel sensors, imaging technology,
and analysis methods, numerous infrastructure platforms have been developed for phenotyping.
Deep Software Variability and Frictionless Reproducibility
Deep Software Variability and Frictionless ReproducibilityUniversity of Rennes, INSA Rennes, Inria/IRISA, CNRS
The ability to recreate computational results with minimal effort and actionable metrics provides a solid foundation for scientific research and software development. When people can replicate an analysis at the touch of a button using open-source software, open data, and methods to assess and compare proposals, it significantly eases verification of results, engagement with a diverse range of contributors, and progress. However, we have yet to fully achieve this; there are still many sociotechnical frictions.
Inspired by David Donoho's vision, this talk aims to revisit the three crucial pillars of frictionless reproducibility (data sharing, code sharing, and competitive challenges) with the perspective of deep software variability.
Our observation is that multiple layers — hardware, operating systems, third-party libraries, software versions, input data, compile-time options, and parameters — are subject to variability that exacerbates frictions but is also essential for achieving robust, generalizable results and fostering innovation. I will first review the literature, providing evidence of how the complex variability interactions across these layers affect qualitative and quantitative software properties, thereby complicating the reproduction and replication of scientific studies in various fields.
I will then present some software engineering and AI techniques that can support the strategic exploration of variability spaces. These include the use of abstractions and models (e.g., feature models), sampling strategies (e.g., uniform, random), cost-effective measurements (e.g., incremental build of software configurations), and dimensionality reduction methods (e.g., transfer learning, feature selection, software debloating).
I will finally argue that deep variability is both the problem and solution of frictionless reproducibility, calling the software science community to develop new methods and tools to manage variability and foster reproducibility in software systems.
Exposé invité Journées Nationales du GDR GPL 2024
3D Hybrid PIC simulation of the plasma expansion (ISSS-14)
3D Particle-In-Cell (PIC) algorithm,
Plasma expansion in the dipole magnetic field.
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Evaporation step counter work. I have done a physical experiment.
(Work in progress.)
Cytokines and their role in immune regulation.pptx
This presentation covers the content and information on "Cytokines " and their role in immune regulation .
EWOCS-I: The catalog of X-ray sources in Westerlund 1 from the Extended Weste...
Context. With a mass exceeding several 104 M⊙ and a rich and dense population of massive stars, supermassive young star clusters
represent the most massive star-forming environment that is dominated by the feedback from massive stars and gravitational interactions
among stars.
Aims. In this paper we present the Extended Westerlund 1 and 2 Open Clusters Survey (EWOCS) project, which aims to investigate
the influence of the starburst environment on the formation of stars and planets, and on the evolution of both low and high mass stars.
The primary targets of this project are Westerlund 1 and 2, the closest supermassive star clusters to the Sun.
Methods. The project is based primarily on recent observations conducted with the Chandra and JWST observatories. Specifically,
the Chandra survey of Westerlund 1 consists of 36 new ACIS-I observations, nearly co-pointed, for a total exposure time of 1 Msec.
Additionally, we included 8 archival Chandra/ACIS-S observations. This paper presents the resulting catalog of X-ray sources within
and around Westerlund 1. Sources were detected by combining various existing methods, and photon extraction and source validation
were carried out using the ACIS-Extract software.
Results. The EWOCS X-ray catalog comprises 5963 validated sources out of the 9420 initially provided to ACIS-Extract, reaching a
photon flux threshold of approximately 2 × 10−8 photons cm−2
s
−1
. The X-ray sources exhibit a highly concentrated spatial distribution,
with 1075 sources located within the central 1 arcmin. We have successfully detected X-ray emissions from 126 out of the 166 known
massive stars of the cluster, and we have collected over 71 000 photons from the magnetar CXO J164710.20-455217.
The use of Nauplii and metanauplii artemia in aquaculture (brine shrimp).pptx
Although Artemia has been known to man for centuries, its use as a food for the culture of larval organisms apparently began only in the 1930s, when several investigators found that it made an excellent food for newly hatched fish larvae (Litvinenko et al., 2023). As aquaculture developed in the 1960s and ‘70s, the use of Artemia also became more widespread, due both to its convenience and to its nutritional value for larval organisms (Arenas-Pardo et al., 2024). The fact that Artemia dormant cysts can be stored for long periods in cans, and then used as an off-the-shelf food requiring only 24 h of incubation makes them the most convenient, least labor-intensive, live food available for aquaculture (Sorgeloos & Roubach, 2021). The nutritional value of Artemia, especially for marine organisms, is not constant, but varies both geographically and temporally. During the last decade, however, both the causes of Artemia nutritional variability and methods to improve poorquality Artemia have been identified (Loufi et al., 2024).
Brine shrimp (Artemia spp.) are used in marine aquaculture worldwide. Annually, more than 2,000 metric tons of dry cysts are used for cultivation of fish, crustacean, and shellfish larva. Brine shrimp are important to aquaculture because newly hatched brine shrimp nauplii (larvae) provide a food source for many fish fry (Mozanzadeh et al., 2021). Culture and harvesting of brine shrimp eggs represents another aspect of the aquaculture industry. Nauplii and metanauplii of Artemia, commonly known as brine shrimp, play a crucial role in aquaculture due to their nutritional value and suitability as live feed for many aquatic species, particularly in larval stages (Sorgeloos & Roubach, 2021).
原版制作(carleton毕业证书)卡尔顿大学毕业证硕士文凭原版一模一样
原版纸张【微信：741003700 】【(carleton毕业证书)卡尔顿大学毕业证】【微信：741003700 】学位证，留信认证（真实可查，永久存档）offer、雅思、外壳等材料/诚信可靠,可直接看成品样本，帮您解决无法毕业带来的各种难题！外壳，原版制作，诚信可靠，可直接看成品样本。行业标杆！精益求精，诚心合作，真诚制作！多年品质 ,按需精细制作，24小时接单,全套进口原装设备。十五年致力于帮助留学生解决难题，包您满意。
本公司拥有海外各大学样板无数，能完美还原海外各大学 Bachelor Diploma degree, Master Degree Diploma
1:1完美还原海外各大学毕业材料上的工艺：水印，阴影底纹，钢印LOGO烫金烫银，LOGO烫金烫银复合重叠。文字图案浮雕、激光镭射、紫外荧光、温感、复印防伪等防伪工艺。材料咨询办理、认证咨询办理请加学历顾问Q/微741003700
留信网认证的作用:
1:该专业认证可证明留学生真实身份
2:同时对留学生所学专业登记给予评定
3:国家专业人才认证中心颁发入库证书
4:这个认证书并且可以归档倒地方
5:凡事获得留信网入网的信息将会逐步更新到个人身份内，将在公安局网内查询个人身份证信息后，同步读取人才网入库信息
6:个人职称评审加20分
7:个人信誉贷款加10分
8:在国家人才网主办的国家网络招聘大会中纳入资料，供国家高端企业选择人才
Nucleophilic Addition of carbonyl compounds.pptx
Nucleophilic addition is the most important reaction of carbonyls. Not just aldehydes and ketones, but also carboxylic acid derivatives in general.
Carbonyls undergo addition reactions with a large range of nucleophiles.
Comparing the relative basicity of the nucleophile and the product is extremely helpful in determining how reversible the addition reaction is. Reactions with Grignards and hydrides are irreversible. Reactions with weak bases like halides and carboxylates generally don’t happen.
Electronic effects (inductive effects, electron donation) have a large impact on reactivity.
Large groups adjacent to the carbonyl will slow the rate of reaction.
Neutral nucleophiles can also add to carbonyls, although their additions are generally slower and more reversible. Acid catalysis is sometimes employed to increase the rate of addition.
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Equivariant neural networks and representation theory
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mô tả các thí nghiệm về đánh giá tác động dòng khí hóa sau đốt
mô tả các thí nghiệm về đánh giá tác động dòng khí hóa sau đốt
bordetella pertussis.................................ppt
bordetella pertussis.................................ppt
Deep Software Variability and Frictionless Reproducibility
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Cardiovascular Research Focus by Proteintech
- 1. 1Cardiovascular Research Focus www.ptglab.com CARDIOVASCULAR RESEARCH FOCUS www.ptglab.com Proteintech offers a wide range of markers and related antibodies for cardiovascular research. IF analysis of (4% PFA) fixed mouse heart tissue using TNNI3 antibody (21652-1-AP) at a dilution of 1:50.
- 2. 2Cardiovascular Research Focus www.ptglab.com CONTENTS Age-associated Changes in Cardiovascular Tissues mTOR in Cardiac Physiology and Disease Cardiac Troponin T Antibody Cardio-related Antibodies $99 Control Antibodies Contact Us 3–4 5 6 7 8 9
- 3. 3Cardiovascular Research Focus www.ptglab.com AGE-ASSOCIATED CHANGES IN CARDIOVASCULAR TISSUES Age-associated changes within the cardiovascular system contribute to deregulation of molecular pathways and pathological alterations. These include: –– Hypertrophy –– Altered left ventricular diastolic function –– Abnormalities in arterial stiffness –– Impaired endothelial function
- 4. 4Cardiovascular Research Focus www.ptglab.com AGE-ASSOCIATED CHANGES IN CARDIOVASCULAR TISSUES Young Heart Mature Heart
- 5. 5Cardiovascular Research Focus www.ptglab.com mTOR IN CARDIAC PHYSIOLOGY & DISEASE Inhibition of mTOR presents evidence for being cardioprotective during aging and cardiac stress. –– Antibody Name mTOR –– Catalog Number 20657-1-AP Product Focus Immunofluorescent analysis of HeLa cells using mTOR antibody (20657-1-AP) at a dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunohistochemistry of paraffin-embedded human testis using mTOR antibody (20657-1-AP) at a dilution of 1:50 (10x objective). MCF7 cells were subjected to SDS PAGE followed by western blot with mTOR antibody (20657-1-AP) at a dilution of 1:500. –– Type Rabbit Polyclonal –– Publications 19 publications –– Applications ELISA, IF, IHC, IP, WB
- 6. 6Cardiovascular Research Focus www.ptglab.com CARDIAC TROPONIN T ANTIBODY cTnT is a cardiac regulatory protein involved in the control of calcium-mediated interaction between actin and myosin. –– Antibody Name cTnT –– Catalog Number 15513-1-AP Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using cTnT antibody (15513-1-AP) at a dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunohistochemistry of paraffin-embedded human heart using cTnT antibody (15513-1-AP) at a dilution of 1:50 (40x objective). Mouse heart tissue was subjected to SDS PAGE followed by western blot with cTnT antibody (15513-1-AP) at a dilution of 1:1000. –– Type Rabbit Polyclonal –– Publications 5 publications –– Applications ELISA, IF, IHC, WB Product Focus
- 7. 7Cardiovascular Research Focus www.ptglab.com CARDIO-RELATED ANTIBODIES Antibody Name Catalog Number Applications Publications ACTA2/smooth muscle actin 14395-1-AP ELISA, FC, IF, IHC, IP, WB 57 CD31 (PECAM-1) 11265-1-AP ELISA, FC, IF, IHC, IP, WB 15 MYL7 17283-1-AP ELISA, IF, IHC, IP, WB 1 Myosin light chain 2 10906-1-AP ELISA, FC, IF, IHC, IP, WB 44 Immunohistochemistry of paraffin-embedded human heart tissue slide using ACTA2 antibody (14395-1-AP) at a dilution of 1:5000 (40x objective). Immunofluorescent analysis of (-20ºC Ethanol) fixed C2C12 cell using ACTA2 antibody (14395-1-AP) at a dilution of 1:50. Immunohistochemistry of paraffin-embedded human lung cancer using PECAM1,CD31 antibody (11265-1-AP) at a dilution of 1:50 (10x objective). Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using MYL2 antibody (10906-1-AP) at a dilution of 1:50. Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using MYL7 antibody (17283-1-AP) at a dilution of 1:50.
- 8. 8Cardiovascular Research Focus www.ptglab.com $99 CONTROL ANTIBODIES Popular tag/control antibodies are just $99 each. GAPDH, beta Tubulin, and 6*His tag antibodies included. See the full list of tag/control antibodies at ptglab.com PROMOTION Corn whole-plant tissue was subjected to SDS PAGE followed by western blot with GAPDH antibody (60004-1-Ig) at a dilution of 1:10000. Immunofluorescent analysis of HepG2 cells using beta- Tubulin antibody (66240-1- Ig) at a dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
- 9. 9Cardiovascular Research Focus www.ptglab.com CONTACT US proteintech@ptglab.com europe@ptglab.com service@ptglab.com Available 24 hours via Live Chat and 9–5 (CDT) via phone. Proteintech Group Proteintech Europe Proteintech Support US Head Office United Kingdom China Office Please visit us at www.ptglab.com for more information about our antibodies and technical tips.