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Botristop at ABIM 2013, Premiere Biocontrol Event
- 1. “Early Stage and Demonstration trial results - Quillaja saponaria extract, effective against Botrytis cinerea in table grapes as an integrated solution for grower´s Botrytis Management Program” Paulina Sepulveda, MSc – INIA Chile ABIM 2013 -Research Collaboration-
- 2. Agenda • Chile in the World • Exports of Table Grapes • Disease: Grey Mold caused by Botrytis cinerea • Available Control Solutions • Our Solution • Experimental and Demostration trials • Summary
- 4. Thompson Seedless Grapes AGRÍCOLA LONQUÉN ISLA DE MAIPO, CHILE Field trials 2011-2012 Early Stage
- 5. Treatment Products Dose Botristop Quillay extract (BS) 2L-Hectare Integrated Boscalid-Botristop-Fenexamid- Botristop- Botristop label Grower Boscalid- Fenexamid-Citric extract-Fenexamid label Control ----- •Randomized Blocks •5 Treatments (4 plants) •3 Replications. Early Stage •5 sprays every 10 days •From veraison to harvest. T1 T5 T2 T4 T3 T4 T2 T3 T1 T5 T1 T4 T5 T2 T3
- 6. • 25 bunches per plant • Incidence (%), • Severity (Scale 0-4) • Disease index • Phytotoxicity Evaluation Methodology Pre harvest
- 7. Incidence of grey mold 05-03-2012: Treatments Percentage c c ab a
- 8. Disease index of grey mold 05-03-2012: Diseaseindex Treatments b ab a b
- 9. Field Trials 2012-2013 Demonstration Thompson Seedless Grapes AGRÍCOLA LONQUÉN ISLA DE MAIPO, CHILE
- 10. Demonstration Treatment Spraying machinery 800L/hectare Field test size: 1 hectare Data sampling: H9 H13 BS400Control H14 H23H18 Grower H19 BS200 H29 H33H28 integrated H24 Treatment Products Dose Botristop Quillay extract (BS) 2L/Hectare Integrated Boscalid–Botristop - Fenexamid-Botristop - Fenexamid-Botristop label Grower Boscalid- Fenexamid— Fenexamid- Citrus extract label Control No applications 4-6 sprays every 10 days from veraison to harvest
- 11. Evaluation Methodology • 75 bunches per treatment • Incidence (%), • Severity (Scale 0-4) • Disease index • Toxicity 1- Pre harvest 2- Harvest • Total weight of infected bunches
- 12. Incidence of grey mold Incidence %, 27/02/2013 b b a b
- 13. Incidence of grey mold Incidence %, 27/02/2013 a b a
- 14. 98,25% Infected grapes Control Botristop Integrated Grower kg/plant 1,838 0,705 0,486 0,534 Fit pre harvest result Avg. kilograms of damaged grapes per plant
- 15. Pos Post Harvest Evaluation • SO2 • Stored fruit at 0°C (30-45 days) • Evaluation of infected berries
- 16. Post Harvest Results Grapes stored at 0°C
- 17. Summary • Quillay extract (BS) represents a new alternative to control Botrytis cinerea. • BS effectively decreased the incidence of Botrytis cinerea when sprayed alone or alternated with chemical fungicides. • BS did not caused phytotoxicity in any of the doses evaluated. • Quillay production platform addresses consistency challenges of formulation´s active compounds.
- 18. “Early Stage and Demonstration trial results - Quillaja saponaria extract effective against Botrytis cinerea in table grapes as an integrated solution for grower´s Botrytis Management Program” -Thank You- V