This document provides an overview of the BOLE program about biodiversity at MacRitchie Reservoir. It defines key terms like biodiversity and describes the different kingdoms of living things found in Singapore like mammals, birds, fish, insects, reptiles, amphibians, arachnids, plants, fungi and microorganisms. It outlines the characteristics and native species within each group. It also discusses life cycles, leaf studies, water quality parameters, biodiversity conservation issues, and provides a briefing on the upcoming nature trail, highlighting animals and plants that may be observed and safety guidelines.