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BIOSYNTHESIS OF SUCROSE
BY
Anju V Narayanan
• Sucrose is common sugar
• It is a disaccharide, a molecule composed of two monosaccharides: glucose
• and fructose
• Sucrose is formed by plants, algae and cyanobacteria but not by other
organisms
• It is found naturally in many food plants along with the monosaccharide
fructose
• In many fruits, such as pineapple and apricot, sucrose is the main sugar
• In others, such as grapes and pears, fructose is the main sugar
• Plants use sucrose as a storage molecule
• For quick energy, cells may store the sugar for later use
SUCROSE
• Sucrose is formed by the condensation polymerization of one
molecule of glucose and one molecule of fructose through
glycosidic linkage
• The linkage is formed between the carbon of the aldehyde group of
the glucose and the carbon of the ketone group of the fructose
• Glucose exists predominantly as a mixture of α and β "pyranose"
isomers, but only the α form links to fructose
• Fructose itself exists as a mixture of α and β "furanose" isomers,
but only the β isomer links to glucose
SUCROSE STRUCTURE
SUCROSE STRUCTURE
• The biosynthesis of sucrose occurs in the cytosol
• The biosynthesis of sucrose proceeds via the precursors UDP-
glucose and fructose 6-phosphate, catalyzed by the enzyme sucrose 6-
phosphate synthase and the conversion of sucrose 6- phosphate to
sucrose catalyzed by the enzyme sucrose 6-phosphate phosphatase
• The energy for the reaction is gained by the cleavage of uridine
diphosphate (UDP)
Continue…..
BIOSYNTHESIS OF SUCROSE
• Photosynthesis carried out by plants, algae and cyanobacteria is the major
source of fixed carbon for all life on earth
• In plant photosynthesis, carbon dioxide is fixed in the chloroplasts via the
Calvin cycle to yield triose phosphates (triose-P)
• Triose-P can be transported to the cytosol by a triose-P/phosphate
translocator
• In the cytosol, two triose-P molecules( Glyceraldehyde 3- phosphate and
dihydroxy acetone phosphate) produce one fructose 1,6-bisphosphate
(F1,6BP) molecule in a reaction catalyzed by aldolase
• F1,6BP is then further metabolized to yield other hexose phosphates, such
as fructose 6-phophate (F6P) and glucose 6-phosphate (G6P)
• Glucose 6-phosphate transforms to glucose 1- phosphate with the help of
enzyme, phosphoglucomutase
• Glucose 1- phosphate can be used to form UDP-glucose (UDP-G)
• Enzyme involved in this reaction is UDP-glucose pyrophosphorylase
• The pyrophosphate produced in the reaction is removed by pyrophosphatase
(2 Pi)
• UDP-G is combined with F6P to form sucrose 6-phosphate (sucrose-P) in a
reaction catalyzed by sucrose 6-phosphate synthase
• Sucrose 6- phosphate is dephosphorylated by sucrose 6- phosphate
phosphatase to form sucrose
Continue…..
2 Pi
Pyrophosphatase
THANK YOU

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BIOSYNTHESIS OF SUCROSE IN PLANTSN BACTERIA

  • 2. • Sucrose is common sugar • It is a disaccharide, a molecule composed of two monosaccharides: glucose • and fructose • Sucrose is formed by plants, algae and cyanobacteria but not by other organisms • It is found naturally in many food plants along with the monosaccharide fructose • In many fruits, such as pineapple and apricot, sucrose is the main sugar • In others, such as grapes and pears, fructose is the main sugar • Plants use sucrose as a storage molecule • For quick energy, cells may store the sugar for later use SUCROSE
  • 3. • Sucrose is formed by the condensation polymerization of one molecule of glucose and one molecule of fructose through glycosidic linkage • The linkage is formed between the carbon of the aldehyde group of the glucose and the carbon of the ketone group of the fructose • Glucose exists predominantly as a mixture of α and β "pyranose" isomers, but only the α form links to fructose • Fructose itself exists as a mixture of α and β "furanose" isomers, but only the β isomer links to glucose SUCROSE STRUCTURE
  • 5. • The biosynthesis of sucrose occurs in the cytosol • The biosynthesis of sucrose proceeds via the precursors UDP- glucose and fructose 6-phosphate, catalyzed by the enzyme sucrose 6- phosphate synthase and the conversion of sucrose 6- phosphate to sucrose catalyzed by the enzyme sucrose 6-phosphate phosphatase • The energy for the reaction is gained by the cleavage of uridine diphosphate (UDP) Continue…..
  • 6. BIOSYNTHESIS OF SUCROSE • Photosynthesis carried out by plants, algae and cyanobacteria is the major source of fixed carbon for all life on earth • In plant photosynthesis, carbon dioxide is fixed in the chloroplasts via the Calvin cycle to yield triose phosphates (triose-P) • Triose-P can be transported to the cytosol by a triose-P/phosphate translocator • In the cytosol, two triose-P molecules( Glyceraldehyde 3- phosphate and dihydroxy acetone phosphate) produce one fructose 1,6-bisphosphate (F1,6BP) molecule in a reaction catalyzed by aldolase • F1,6BP is then further metabolized to yield other hexose phosphates, such as fructose 6-phophate (F6P) and glucose 6-phosphate (G6P)
  • 7. • Glucose 6-phosphate transforms to glucose 1- phosphate with the help of enzyme, phosphoglucomutase • Glucose 1- phosphate can be used to form UDP-glucose (UDP-G) • Enzyme involved in this reaction is UDP-glucose pyrophosphorylase • The pyrophosphate produced in the reaction is removed by pyrophosphatase (2 Pi) • UDP-G is combined with F6P to form sucrose 6-phosphate (sucrose-P) in a reaction catalyzed by sucrose 6-phosphate synthase • Sucrose 6- phosphate is dephosphorylated by sucrose 6- phosphate phosphatase to form sucrose Continue…..