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Introduction
The insurmountable rising cost of inorganic fertilizers is inevitably uncontrollable in the
coming production years. Looking into this perspective the farmers has to look for an alternative
measures to sustain his farming business profitability.
On December 27, 2005 Her Excellency President Gloria MacapagalArroyo signed
Executive Order 481 on the Promotion andDevelopment of OrganicAgriculture in the Philippines.
Then, Former Agriculture Secretary Domingo F. Panganiban signed Administrative Order No. 9
series of 2006 or the Implementing
Rules and Regulations (IRR) of EO 481. With these development the Department of
Agriculture has come up with programs and projects in support to EO 481. Thus, the Agricultural
Training Institute in Region 8 made this material on bio-organic inputs in response to the
program.
This material discusses steps on the preparation, the materials needed, the applications
and benefits of the different bio-organic inputs. It is hope that this material will provide the
needed knowhow on bio-organic inputs that would eventually help farmers improve their
farming.
Objectives
1. To produce homemade BIO FERTILIZERS using inexpensive basic materials.
2. To identify its different use and applications.
3. To make recommendations to improve the production and to maximize its effectivity.
The Bio-Organic Inputs
This material discusses the following organic inputs:
1. Lactic Acid Bacteria Serum (LABS)
2. Fermented Amino Acid (FAA)
3. .Vermitea (VCT)
4. Oriental Herbal Nutrients (OHN)
5. Fermented Plant Juice (FPJ)
6. Fermented Fruit Juice (FFJ)
7. Indigenous Microorganism (IMO)
LACTIC ACID BACTERIA SERUM
Lactic acid bacteria (LAB) are widespread microorganisms which
can be found in any environment rich mainly in carbohydrates, LAB are
anaerobic microorganisms that decompose sugar in the absence of
oxygen. Normally, they are separated and cultured with rice washed
water and milk. This is how Lactic Acid Bacteria Serum (LABS) is obtained.
Lactic Acid Bacteria Serum is now used for its ability to convert
waste into organic matter and basic materials. And they thrive and feed
on the ammonia released in the decomposition normally associated with
the foul odor (removes foul odor). Lastly, they serves as defense against
pathogenic diseases such as harmful viruses and fungi.
Today LABS application can be observe on fieldof natural farming,
livestock production, aquaculture, and even in households.
MATERIALS TO BE USED:
 1kg Rice
 Plastic Basin
 Water  1 kg of Powdered Milk
 1 Kg of Molasses  Strainer (or Clean cloth)
 Paper and Rubber band  Funnel
 Jar or Container
PROCEDURES:
STEP 1: Put 700 ml of water to 1 kg rice then by use of hand stir, swirl and crush the mixture
until milky colored water is attained. (The water is now a rich source of carbohydrates) Obtain
rice wash by filtering the mixture using clean cloth.
STEP 2: Place 500 ml rice wash to a jar and cover it with paper (use rubber band to tie the paper
covering). Leave the jar in cool dark place for 5-7 days.
STEP 3: After a week, when the bran has risen and it smells a little sour and forms 3 layers. This
is indicating the rice wash is infected with various microbes. Take about 100 ml of clear water
by straining again the mixture.
STEP 4: Add 1 L of milk (to obtain 1:10 Water and Milk ratio). You may use skim or powdered
milk, although fresh milk is the best. Cover the jar with paper same as before. Leave for 5-7
days in cool dark place.
STEP 5: After about 1 week you’ll see curds (made of carbohydrate, protein, and fat) or the
white part and yellow liquid or whey, enriched with lactic acid bacteria from the fermentation
of the milk. Extract the whey by pouring through a strainer
STEP 6: Add Molasses or brown sugar in equal part to the collected whey. This is to preserve
the LABS at room temperature. So, if you have 1L of serum, add 1kilo sugar or 1L molasses.
Otherwise store in fridge to keep. Then place it in clean container and label it accordingly
USES AND APPLICATION OF LABS
Based on our research we summarizes the different uses of LABS:
 It can be used as alternative strategy to antibiotics growth promoters used in farm
animals. Because continuous use of antibiotics may increase bacterial resistance, which
can threaten the health of both animals and humans. Mix 2tbsp to 1L water, then add
that mixture to animal’s water at 2tbsp/L (so the animal’s water contains little less than a
quarter tsp/L of lacto serum). But this is very flexible. The Lacto serum is not harmful, so
it’s just about adding enough to be effective, without wasting it.
 In plants, spraying diluted solution of lactic acid bacteria serum (2tbsp. /1 liter of water)
to the plant and soil helps plant growth and makes them healthier. As it is applied to the
soil or the leaves, these beneficial bacteria aid in the decomposition process, thus
allowing more food to be available and assimilated by the plant. And it is also known to
produce enzymes and natural antibiotics aiding effective digestion and has antibacterial
properties that serves as defense against fungi and viruses.
 In Aquaculture, with regular addition of this beneficial organism to poor quality water
(becauseof high amount of ammonia that pollutes water) ammonia problem is minimized
and helps hasten or complete the denitrification or converting wastes into forms not
harmful to fish. Add lacto at roughly 1L per 700m3 of fish-containing water.
 Other applications: Used for its odor killing ability in animal wastes, clogged drains, foul
odor of canals and addition to beneficial organism in composts.

RECCOMENDATION
We recommend that:
1. Use LABS in morning, to avoid exposure to direct sunlight with UV that may kill or
reduce their effectivity.
2. Avoid store milk that have antibiotic components.
3. During production and storage keep away the container from rainwater for this may
cause contamination. Also keep it safe from wild animals.
4. Do not use plastic lids or covering that will prevent aeration. That’s why we use paper.
5. Use gloves and protective gears, aside from LAB it is possible that it contains harmfull
organism that may enter your body through exposed body parts (ex. wounds)
REFERENCES
Ikeda, David M. et al. Sustainable Agriculture, “Farming: Lactic Acid Bacteria”.
August 2013, CTHR. Minoa, Hawaii
Gilliland, S. E., 1990. Health and nutritional benefits from lactic acid bacteria. FEMS Microbiol
Rev. 1990 Sep;7(1-2):175-88.
Lim, A. K., 2005. Handout on natural farming system and technology seminar. Davao: Tribal
Mission Foundation International, Inc.
Guolong Zhang, The Use of Lactic Acid Bacteria as a Probiotic in Swine Diets, Mar. 2015 URL:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4384071/
Rory Turnbull, DIY Lacto Bacillus Culture, Jan. 2013 URL:
https://permies.com/t/25812/permaculture/DIY-Lacto-Bacillus-Culture
Gil and Patrick, 2016 URL:
http://theunconventionalfarmer.com/recipes/lactobacillus-serum/
Dugas, J., n.d. Lactic acid bacteria. URL:
http://www.waksmanfoundation.org/labs/mbl/lactic.html.
FISH AMINO ACID
In “Natural Farming” natural materials are used instead of chemical inputs. One of the
important tools used in Natural farming is Fish Amino Acid or FAA. These kind of bio fertilizers
are easily made and prepared and help to reduce expenses of farmers.
The Fish Amino Acid (FAA) is a liquid made
from fish. FAA is of great value to both plants and
microorganisms in their growth, because it
contains various types of amino acids and
abundant amount of nutrients. Studies have
proved that Amino Acids can directly or indirectly
influence the physiological activities of the plant.
Amino Acids are also supplied to plant by
incorporating them into the soil. It helps in
improving the micro flora of the soil thereby
facilitating the assimilation of nutrients.
FAA is produced by fermenting fresh fish by-products (bones, head, skin, and other
tankage parts) with brown sugar. Fresh fish scraps is collected and composted in a bucket added
with molasses to the mixture to help build up microbes, and speed up decomposition. The brown
matter molasses controls the odor and absorbs organic nitrogen from the fish. The bucket of fish
is then made to rot for at least one week. The liquid is used to breed beneficial microbes and
supply extra proteins to the emulsion.
It provides an NPK ratio of 4-1-1 and is most often used as foliar feed to provide a quick nitrogen
boost.
MATERIALS NEEDED IN MAKING FISH AMINO ACID
1. 1 kilo of Fish trash (head, bone, intestines, scales, gills, etc)
2. 1 kilo of molasses
3. Jar or Contatiner
4. Paper and String/Rubber Band
5. Bottle for storage
STEP BY STEP PROCEDURE
1. Collect a kilo of Fish Trash. And cut into pieces
2. Place the Fish Trash inside the Jar.
3. Put in a kilo of molasses in the Jar and mix.
4. Cover the Jar with paper and tie it with the string.
5. Place the jar in a cool dry place. Away from sunlight and rain. Leave it there for 20 days.
6. After 20 days, separate the liquid from the solids. The liquid has the Fish Amino Acids
(FAA).
7. Transfer liquid into clean plastic bottles.
USES AND RATES OF APPLICATION OF FERMENTED FRUIT JUICE
PROPER USAGE: 1-2tsp per Liter of water
 Plants- Aside from organic nitrogen sources, they also supply phosphorus, potassium,
amino acids, proteins and trace elements or micro-nutrients that are really needed to
provide deep nutrition to your soil community and plants. One of the benefits of fish
emulsion is that they provide a slower release of nutrients into the soil without over-
feeding all at once.
 What more, using fish emulsion as our foliar spray helps us get rid of pests too. They
hate the fishy smell, no matter how miniscule.
 Soil – (1) Use as a soil drench as opposed to foliar spray. Whenever we drench the soil
with fish, we build up the soil’s microbial activities, supplying lots of nitrogen to the
roots and topsoil. (2) Inoculate compost to boost fungal population. (3) (4) Mix in water
when watering plants, as a natural fish fertilizer and to enhance populations of micro-
organisms in the soil
 Animals- Mix with water for an effective protein/lacto boost for your animals. They'll
love or hate it
TIPS AND SUGGESTIONS
 The bucket is stirred daily to get air into the mixture. Remember air allows for better
decomposition and better aerobic microbial growth.
 You will likely be able to get fish scraps or wastes in places where fish is cleaned prior
selling or consumption. The fish section of the wet market will usually have traders
removing fish parts like intestines, gills, liver, gall bladder, heart, fins, tail, scales and
even bones. Typically, these are thrown away and so you could just ask them for free.
 The pulp that will be left behind after extracting and collecting the fish emulsion is not
wasted. It may be used as a good starter mix for the next batch of fish emulsion,
because of its state of decomposition. It may also be used to feed a compost pile that
can later be applied and mixed into your garden soil.
 Never use directly to plant leaves or soil, you should dilute it with water. you should
dilute fish amino acid with water.
 It is not recommendable to use FAA during the period of reproductive growth, because
it may induce over growth.
 The optimum temperature for storage ranges between 23-250C andNo direct sunlight.
Acool place is recommended.
REFERENCES
http://www.downtoearth.ph/pages/Fish-Emulsion-as-Fertilizer-and-Pesticide.html
http://www.practicalgardening.blackdovenest.com/2012/03/easy-homemade-fish-emulsion-
fertilizer.html
http://www.ridgedalepermaculture.com/blog/making-fish-hydrolysate
http://www.gardeningknowhow.com/garden-how-to/soil-fertilizers/fish-emulsion-
fertilizer.htm
http://farming.pinakbet.net/2011/12/how-to-make-fish-amino-acid-faa/
http://www.ctahr.hawaii.edu/oc/freepubs/pdf/SA-12.pdf
VERMITEA
The presence of earthworms in soil ecosystems is
an indicator of the well- being of a system. Earthworms
have the unique ability to convert elements such as
minerals, nutrients and microbes from the soil or
composting systems into an excretion that contains the
vastest amount of plant nutrients, microbes and growth
elements that do exist. The excretion is called vermicast or
vermicompost.
Vermicompost is a compost that has been digested
by worms (vermi) and does not need to be turned, because
worms "turn" the organic matter in their digestive tract,
eliminating work for gardeners.
Vermicompost tea is a liquid derived from soaking
and or brewing compost in water and is used as a foliar and
soil application to plants. Vermicompost tea protected the
leaves and stems of plants from the top down and not only from the soil. Direct application on
the plant dramatically boosted its growth and vitality. The extract was rich in microbes, which
helps restore plants after being exposed to pesticides and other environmental damage. It can
contain the three basic plant nutrients: nitrogen in the form of nitrate or ammonium (N03 and
NH4); phosphorus (P); and potassium (K).
MATERIALS NEEDED IN MAKING VERMITEA
1. Large clean bucket,
2. High quality vermicompost / worm castings
3. Some type of permeable bag like nylon material (such as nylon stockings) for holding
the compost;
4. Aged water – if you are using tap water you should let it sit for a day or two so as to
remove the chlorine. Preferably, use some rainwater or pond water if you have some on
hand.
5. A bucket
STEP BY STEP PROCEDURE
1. Dissolve 2kg of molasses on the 200L water placed in a container
2. Put 10kg vermicast in a nylon stocking or similar permeable bag. .
3. Tie off nylon, and dip and submerged in water-molasses mixture. Cover the
container to keep away from insect and animals. Place the brewing in a shaded area
out of direct sunlight
4. After 3 days remove the vermicast sack. Mix the water mixture and this will be the
Vermitea. Harvest and transfer to clean containers.
USES AND RATES OF APPLICATION OF VERMITEA
Use a ratio of 10 parts water to 1 part tea
 Disease- For disease suppression on fruits, vegetables and ornamentals, applications can
be made as often as every 5 to ten days.
 As A Root Drench- Can be used unfiltered by applying directly to the soil area around a
plant. The tea will step down into the root system. Root feeding is not affected by rainy
weather.
 Root Zone- Spray it over the root zone to feed the plant through the roots. I prefer to
put compost tea in a watering can and water the leaves. A good amount drips off to
enrich the soil. This is fast, easy, and effective.
 Transplant Solution- Another great use for compost tea: use it as a transplant solution,
to help your plants in transitioning to their new environment
 Compost Pile- If you have any tea left over, consider putting it on your compost pile.
This can help accelerate the microbial activity of your compost pile, which means
quicker composting!
 Plant. - Compost Tea can be applied about every two weeks to your garden plants once
plants and transplants have become established. By established, we simply mean that
they have been in the ground 7 to 10 days and are over the initial shock of transplant.
TIPS AND SUGGESTIONS
 Apply VCT early in the day, to allow the foliage to dry before night, which reduces the
chance of foliar diseases
 Use well-aged, finished compost
 Unfinished compost may contain harmful pathogens and compost that is too old may be
nutritionally deficient. COMPOST TEA and MANURE TEA ARE NOT THE SAME THING!
Manure teas may be made in the same way but are not generally recommended as foliar
sprays and are not as nutritionally well-balanced.)
 If molasses are not readily available, you can use brown sugar.
 Best if you put out the sack of vermicast 3 times a day.
 Water your garden as you normally would
 Water your houseplants
 Use the worm compost tea to water seedlings or baby plants
 Cover a whole plant with worm compost tea including the leaves. Many people believe
that the beneficial microbes in worm tea help protect plants from diseases. A sprayer or
spray bottle works well. Be sure to strain the worm compost tea before adding it to the
spray bottle.
 Serve worm compost tea at your next tea party.
REFERENCES
http://www.homecompostingmadeeasy.com/composttea.html
http://www.healthy-vegetable-gardening.com/using-compost-tea.html
http://farming.pinakbet.net/2013/02/how-to-make-vermi-tea/
http://www.extension.umn.edu/garden/yard-garden/soils/vermicompost-tea/
http://www.ijsrp.org/research-paper-0416/ijsrp-p5276.pdf
ORIENTAL HERBAL NUTRIENT
Oriental Herbal Nutrient (OHN), a fermented
extractof herbs,is usedin Natural Farmingto provide
plants and soil microorganisms with micro-nutrients,
which may optimize their resilience to environmental
stresses (wind, heat, drought, etc.). OHN is a mixture
of edible, aromatic herbs extracted with alcohol and
fermented with brown sugar. It is used to discourage
the growth of anaerobic, potentially pathogenic
microbesandencourage beneficialaerobicmicrobesin
the soil and on plants.). These herbs are used in the
creation of OHN.
MATERIALS NEEDED IN INDIGENOUS MICRO ORGANISM
1. 3 or 5 of Plant material examples are: Garlic, Ginger, Onion, Siling Labuyo, Oregano etc.
2. Beer
3. Gin or CoconutVinegar
4. BrownSugar or Molasses
5. Jar or container
6. Paper and Rubber Band
STEP BY STEP PROCEDURE
1. Chopequal amount of choice of herbs(Ex.1kg of Garlic, Gingerand Onion). Anycombinationof
the above will work. Aneasierwayinsteadof choppingispressing,hammeringthemtobreakthe
fibers. Place in the jar or container. Add beer to the level of the ingredients. Cover with paper
and ensure with rubber band, keep in a dark, cool place. Leave for 12 hrs.
2. Add Molasses the amount of used individual herb (1kg molasses using above example) .Cover
again with paper/rubber band and keep in a cool, dark place. Ferment for 7days.
3. AddGinorCoconutVinegar,equivalenttothe amountof Beerthatwasusedonday1.Coveragain
and keep in a dark, cool place.
4. After10 days.Time to harvestthe OHN.Justfilteroutthe sludge.Thisisconsideredthe firstcycle
5. Repeat Step 2-4 for the 2nd cycle
6. Repeat Step 2-4 for the 3rd cycle
USES AND RATES OF APPLICATION OF IMO
PROPERUSAGE: 1-2tsp perLiterof water
 PEST AND DISEASES
o It's primarilyusedasanIPMinput.ItkillsPMand downymoldverywell,promotinggood
colonization of microbes.
o Apply as a foliar spray on plants to repel insects. Apply during late afternoon or early
morning hours.
o When plants have soft rot or anthracnose add water soluble calcium (WCA 1000)
to this solution.
 ANIMAL
o OHN keeps our chickens healthy. Use it 3x a week to strengthen their immune system.
Whenthe chickensare sick,theyare givenOHN for3daysstraight. Use 2Tablespoonsfor
every liter of fresh water.
 SOIL
o Apply diluted OHN as a soil drench prior to planting to activate dormant soil
microorganisms.
 PEST AND DISEASES
o It's primarilyusedasanIPMinput.ItkillsPMand downymoldverywell,promotinggood
colonization of microbes.
o Apply as a foliar spray on plants to repel insects. Apply during late afternoon or early
morning hours.
o When plants have soft rot or anthracnose add water soluble calcium (WCA 1000)
to this solution.
REFERENCES
http://www.ctahr.hawaii.edu/oc/freepubs/pdf/SA-11.pdf
http://www.pinoybisnes.com/agri-business/natural-farming-technology-and-its-techniques/
http://www.cgnfindia.com/ohn.htmlhttp://solraya.blogspot.com/2011/02/oriental-herbal-nutriets-
ohn.html
FERMENTED PLANT JUICE
Fermented plant juice (FPJ) is
derivedfrommixingthe youngshootsof the
plants with molasses and/or crude sugar
and fermented in one (1) week. The shoots
of plantsare difficulttodissolve inwateror
anykindof oil butitcanbe done withalittle
amount of alcohol. The process of
fermenting the soft part of the plants with
molasses/crude sugar and with the
presence of microorganisms will result to a
small percentof alcoholwhichisresponsible
in extractingthe juice from the young parts
of the plants. FPJ is rich in micro and macro-nutrients. The primary elements that FPJ can provide are
nitrogenandsome micro-nutrientslikecalcium,molybdenum, manganese,ironandcarbon.The juice also
contains rich microorganisms which give strength to plants and animals
MATERIALS NEEDED IN MAKING FERMENTED PLANT JUICE
1. The part of the plantsusedfor this fermentationisthe shootbecause it is thispart that storesa
high percentage of nutrients coming from the soil and from the atmosphere. Moreover, plants
usedforFPJare those thatare fastgrowingsuchthat,if you cut the shootnow,regenerationof a
newpart will take effectinafewhours. We ourselvesuse: Kamote,Squash,Kangkong,Alugbati,
Banana,Bamboo. You may use otherswithsame characteristicsandthe onesreadilyavailablein
your area.
2. Youcan use eithercrude sugarormolassesorwhicheverisavailableandcanbe boughtatalower
price.
3. You will need basin, ceramic pot or plastic pail, net bag or cloth bag, paper or cloth for cover,
string, stone as weight, bolo, chopping board, marking pen and glass jars.
STEP BY STEP PROCEDURE
1. Shake off dirt from the Plants but do not wash in water. Washing will remove useful
microorganisms.If the ingredientsare too big,cut themto adequate sizes,about3 to 5 cm. This
increases contact surface area and promotes osmotic pressure.
2. Measure the weight of the ingredient and the weight of brown sugar. Brown sugar should be
between half of the weight of the ingredient.
3. Put the ingredientsandbrownsugarin a large wide containerandmix.Coverwithporouspaper
and leave for 1 to 2 hours.
4. Put the mixture intothe jar.It shouldfill up¾of the jar. It is importantthat the jar is nottoo full
or under full. The empty space is not empty. It is filledwith air, for optimum fermentation to
occur.
5. Puton the cover and tie the jar. A coverisneededtopreventinsectsfromgettingintothe mixture.
Paper is ideal because it lets the air in and out.
6. Remove the weight after 1 or 2 days. After the air has escaped, put back the cover again.
7. Putthe jarin a cool and shaded place.Donotopen,move orstirthe ingredientsduringthe process
of fermentation.
8. After15days,strainthe contentsof the pail andtransferliquidtoacleancontainer. Younowhave
Fermented Plant Juice which is a very good fertilizer.
USES AND RATES OF APPLICATION OF FERMENTED PLANT JUICE
 As seed treatment before sowing – soak the seeds in 0.2 % solution for 4 to 5 hours to facilitate
germination and as a start-up solution to germinating seeds.
 As a natural growthenhancer– FermentedPlantJuice made fromactivelygrowingplantpartsand
fastgrowingplantsmaycontainnatural growth hormonesandmineralizednitrogenthatpromotes
plantgrowth.Mix 1 teaspoonof FermentedPlantJuice perliterof waterandsprayonthe leavesor
applydirectlyto the soil around the plantsfrom seedlingstage upto pre-floweringstage.Youcan
applyweeklyordependingonplantvigor.Please note thatwith the use of FermentedPlantJuice,
there is no overdose; you may use it liberally. However, the soil must be watered first before
applying Fermented Plant Juice to avoid scorching of the roots.
 Apply Fermented Plant Juice to the soil to serve as source of energyto accelerate activities of soil
microorganism. This activity will make the nutrients available to the plants.
 Give Fermented Plant Juice, as drink, to livestock at 1 tbsp/liter to increase microbial activities in
gastrointestinal tracts. This would result to better absorption of nutrients.
 Spray to animal beddings to hasten manure decomposition.
TIPS AND SUGGESTIONS
o The FPJenhancesgrowthandprovidesfoodforthe indigenousmicroorganisms(IMO). We addit
to the drinkingwaterof our chickensandsprayit all aroundthe farm on the seedlings,vegetable
plots and fruit trees.
o The mostimportantrequirementwhenselectingplantsformakingFPJis to use the growingtips
of plantspeciesthatare fastgrowers.Flowers,flowerbuds,andimmature fruitscanalsobe used.
Hard orwoodyplantpartswill yieldlittleornoplantjuice.The plantsshouldbe vigorouslygrowing
at the time of collection.Plantpartsshouldbe harvestedwhilethe plantsare inrespirationmode
(before sunrise) and not in photosynthetic mode (during daylight), due to the effects these
processes have on plant chemistry.
o Polyethylene or glass products or clay jar may be used as a container. When usingglass bottles,
brown glass containers must be preferred.
o Store in a cool place. Select a shaded area where there is no direct sunlight and where the
temperature does not fluctuate. Direct sunlight should be avoided.
o The optimum temperature range is 1 to15ºC for storage (Use a Refrigerator if available) if you
want to keep for one year. Otherwise one can use within 30 days store at room temperature.
o WHEN NOT TO USE FPJ
 Duringexcessivevegetative growthorovergrowthdue toprolongedrainsorcloudyweather.
 FPJ made from the crop itself should not be used as this will promote further growth.
 During acidic or nitrogen-excessive crop conditions, which create a pest-attractive
environment.
 Conditions of too much moisture or of bad ventilation, which promotes fungal growth.
REFERENCES
http://businessdiary.com.ph/3468/how-to-make-fermented-plant-juice-or-fpj/
http://www.cgnfindia.com/fpj.html
http://www.freewebs.com/jumblebox/blogF/jumbleblog/fertilize2.html#.WDoLu9IrLcc
http://www.agnet.org/library.php?func=view&id=20110804162833
http://solraya.blogspot.com/2010/09/fermented-plant-juice-fpj.html
http://naturalfarminghawaii.net/wp-content/uploads/2013/09/Fermented-Plant-JuiceFPJ.pdf
FERMENTED FRUIT JUICE
Fermented fruit juice, also known as FFJ, is
a partof the ‘Natural farmingmethod’developedby
Dr. HanKyuChoof SouthKorea.Thisconcoctioncan
be made at home from many common overripe
sweetfruits.Itusesbrown(raw)sugartoextractthe
phytochemicals in fruits. The fermenting process
takes at least a week to complete. This liquid have
nutritional activation enzymes and help to increase
nutrients in the soil and therefore in the plants
growing in it, especially potassium levels. It is
commonly used as a foliar spray to enhance
floweringandfruitingstage.Now,itisalsouse inanimal productionasitgivessupplemental nutrientsto
animals.
MATERIALS NEEDED IN MAKING FERMENTED FRUIT JUICE
1. Locallyproducedsweetripe fruitslike mango,banana, papaya,strawberryandchico;ripe squashfruit
and matured carrot; and root crops particularly camote, cassava and gabi. Citrus fruits are not
recommended.You can make Fermented FruitJuice fromsingle material or a combination of materials.
The extract from the combination of banana, papaya, and squash have been proven to be effective in
flower induction and fruit setting by many organic farmers.
2. Youcan use eithercrude sugarormolassesorwhicheverisavailableorcanbe purchasedatlowercost.
3. You will also need ceramic pots or plastic pail, basin, net bag or cloth bag, paper or cloth for cover,
string, stone as weight, bolo, chopping board, marking pen, and glass jars for storage.
STEP BY STEP PROCEDURE
1. Prepare the Ripe Fruit Meat by peeling the skin and removing the seed of the fruit.
2. Chop the Ripe Fruit Meat into little pieces.
3. Put chopped materials in the jar and add exact amount/weight of crude sugar or molasses, and
then mix. You must make sure that all chopped materialsare coated with sugar or molasses so
that the juice can be extracted easily.
4. Cover the jar with paper and tie it securely with the string.
5. Leave the jar in a cool and dry place for 7 days to ferment. Away from sunlight and rain. Make
sure that the storage area is not infestedwithcockroachesormice,because theymightfeedon
the mixture and contaminate the extract. In 7 days, plant juice is extractedand fermented. The
fruitextractwill change itscolorfromyellow orangetobrown,andwillsmell sweetandalcoholic.
6. After 7 days, separate the liquid from the solids. The liquid is the Fermented Fruit Juice (FFJ).
USES AND RATES OF APPLICATION OF FERMENTED FRUIT JUICE
 Asflowerinducerandfruitsetter –FermentedFruitJuice madefromacombinationof ripe fruits
of banana,papayaand squashhave beenprovenbymany organicfarmersto be effective when
sprayedon the leavesatthe rate of 2 to 4 tbsp./gallonof waterat the onsetof floweringupto
fruit setting. These ripe fruits contain phosphorous and potassium which are necessary during
the flowering and fruit setting stage.
 As soil microorganism activity accelerator – Fermented Fruit Juice is applied directly to the soil
at the rate of 1tsp/literof water.The carbohydratesandsugar contentof Fermented FruitJuice
serve as source of energy of soil microorganism, thereby, accelerating their activity.Increased
microbial activities result to the availability of nutrients for plant’s uptake.
 As spray to animal beddingstohastenmanure decomposition –FermentedFruitJuice contains
beneficial microorganisms that help in the decomposition process.
 As a nutritious drink – a 20% Fermented Fruit Juice solution makes an excellent drink for both
human and livestock.
TIPS AND SUGGESTIONS
o Use any materials that are free from insect pests and diseases.
o Avoid using fruits that were treated with Calcium Carbide (kalburo) or any other chemical
treatments.
o Do not use white sugar.
o Youdo nothave tomix the brownsugarwithwatertomake itliquid.The risein temperaturedue
to fermentation will melt the brown sugar.
o If jars are too expensiveorare not readilyavailable inyourlocationyoucanuse plasticwater
containers.
o Always leave 1/3 of the bottle empty so the liquid can “breathe”
o Don not tighten the bottle lid for 2 weeks after bottling to allow gasses to escape and avoid a
sticky explosion
o Solid materials from the fermentation process can be given to animals as feeds or to plants as
compost
o FFJ should have a pleasant smell and sweet and tangy taste
o FFJ keeps for over a year or longer if refrigerated
REFERENCES
http://businessdiary.com.ph/3468/how-to-make-fermented-plant-juice-or-fpj/
http://www.cgnfindia.com/fpj.html
http://jumblebox.webs.com/blogF/jumbleblog/fertilize3.html#.WDeAiLIrKM8
http://www.pinoybisnes.com/food-business-ideas/how-to-make-fermented-fruit-juice-for-natural-
health/
http://homeguides.sfgate.com/make-fermented-fruit-plant-food-63700.html
http://theunconventionalfarmer.com/recipes/bloom-fertilizer/
INDIGENOUS MICRO ORGANISM
IndigenousMicro-Organism(IMO) isone
of the most basic and important input in natural
farming. IMO are naturally occurring microbes
from bacterial and fungal sources that have
uniquelyevolvedtothrive inyourparticulararea
and are collected and used to re-populate the
soil's micro-sphere and the plant's surfaces -
bringing them back into balance. This balance
serves as the foundation for a healthy soil
biology, productive nutrient recycling, and
optimized plant growth/production. A large
diversityof IMOcan be foundinforests,bamboo
grovesandareaswiththickaccumulationofplant
residues. Advantages of IMO are: (1) Organic &
chemical-free Safe, (2) cheap and easy to use. (3) Nature & environmental friendly
MATERIALS NEEDED IN INDIGENOUS MICRO ORGANISM
1. Container(bamboosplitopenorwoodenbox/plastictrayabout81/2”x11”x3”)
2. Manilapaper
3. String
4. 1 kg cookedcool rice
5. 1 kg molasses
6. Plasticbag
7. Plasticpail
STEP BY STEP PROCEDURE
1. Weigh1 kg of cookedcool rice and place inside achosencontainer.
2. Coverthe containerwithcleanManilapaper;tie withstringthenwrapwithplasticbag.
3. After3 days checkif there are white moldsontopof the rice. If none yet,returnandkeepit
there until there are enoughmolds.
4. Remove the moldyrice fromthe container,place inaplasticpail and add1 kg molasses. Be sure
that the containerisonly70% full.
5. Coverthe pail witha cleansheetof paperand store ina drycool place,awayfromsunlight.
Label accordingly.
6. After7 days,the mixture willyieldamudlike juice,youcanremove the liquid.
7. Label withthe name of the mixture,the startof the processand the date of harvest.
8. Strainand bottle.
USES AND RATES OF APPLICATION OF IMO
PROPERUSAGE: 1-2tsp perLiterof water
 EnrichesSoil -Usingof IMO buildsupthe soil’slife anddiversity.Theiressential workistobreak
downnutrientsintoformsthatare accessible toplantsandanimals.
 Protectscrops fromdiseases- The more sterilizedyoursoil isthe fewermicroorganismsitwill
have,thiswill invitesdiseasescausingthemtopropagate explosively.A webof livelyanddiverse
microorganismsprovidesthe necessarychecksandbalancespreventingthisfromoccurring.
 Plants- Applyasa foliarsprayor soil drench.Greatlyenhancesgrowthandhealthof plantsby
establishingahealthypopulationof microbesinthe soil andonleaf surfaces.Transportsfoodto
roots buildsahealthyecosystemfromthe groundup.Thisisan invaluable jobandthe greatest
benefitof thisserum.Aidsdiseaseresistance –fightspathogens,occupiesspacesthatcould
otherwise gotoharmful bacteria/molds.Aidcomposting –massivelyenhancescompost –there
will be a whole separate postonthisconceptAidorganicfertilizer.Addtoyournutrient
solution,microbesbreakdownorganicnutrientsintobio-availableformsthatplantscanutilize
directly
 Animals - Boostgrowthby enhancingdigestionandaidsdisease resistance.Fightthe bad
bacteria.Usedto inoculate farmyard(sprayground) where animalsoccupytomaintainhealthy
microbial system.Usedtocreate andmaintaina healthydeepbedlitterflooring.Itisthese
micro-organismsthatwill helpbreaksdown fecal matterrapidlyleavingyouwithafertilizerrich
material whilstensurenosmellsorflies

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BIOFERTILIZER

  • 1.
  • 2. Introduction The insurmountable rising cost of inorganic fertilizers is inevitably uncontrollable in the coming production years. Looking into this perspective the farmers has to look for an alternative measures to sustain his farming business profitability. On December 27, 2005 Her Excellency President Gloria MacapagalArroyo signed Executive Order 481 on the Promotion andDevelopment of OrganicAgriculture in the Philippines. Then, Former Agriculture Secretary Domingo F. Panganiban signed Administrative Order No. 9 series of 2006 or the Implementing Rules and Regulations (IRR) of EO 481. With these development the Department of Agriculture has come up with programs and projects in support to EO 481. Thus, the Agricultural Training Institute in Region 8 made this material on bio-organic inputs in response to the program. This material discusses steps on the preparation, the materials needed, the applications and benefits of the different bio-organic inputs. It is hope that this material will provide the needed knowhow on bio-organic inputs that would eventually help farmers improve their farming. Objectives 1. To produce homemade BIO FERTILIZERS using inexpensive basic materials. 2. To identify its different use and applications. 3. To make recommendations to improve the production and to maximize its effectivity. The Bio-Organic Inputs This material discusses the following organic inputs: 1. Lactic Acid Bacteria Serum (LABS) 2. Fermented Amino Acid (FAA) 3. .Vermitea (VCT) 4. Oriental Herbal Nutrients (OHN) 5. Fermented Plant Juice (FPJ) 6. Fermented Fruit Juice (FFJ) 7. Indigenous Microorganism (IMO)
  • 3. LACTIC ACID BACTERIA SERUM Lactic acid bacteria (LAB) are widespread microorganisms which can be found in any environment rich mainly in carbohydrates, LAB are anaerobic microorganisms that decompose sugar in the absence of oxygen. Normally, they are separated and cultured with rice washed water and milk. This is how Lactic Acid Bacteria Serum (LABS) is obtained. Lactic Acid Bacteria Serum is now used for its ability to convert waste into organic matter and basic materials. And they thrive and feed on the ammonia released in the decomposition normally associated with the foul odor (removes foul odor). Lastly, they serves as defense against pathogenic diseases such as harmful viruses and fungi. Today LABS application can be observe on fieldof natural farming, livestock production, aquaculture, and even in households. MATERIALS TO BE USED:  1kg Rice  Plastic Basin  Water  1 kg of Powdered Milk  1 Kg of Molasses  Strainer (or Clean cloth)  Paper and Rubber band  Funnel  Jar or Container PROCEDURES: STEP 1: Put 700 ml of water to 1 kg rice then by use of hand stir, swirl and crush the mixture until milky colored water is attained. (The water is now a rich source of carbohydrates) Obtain rice wash by filtering the mixture using clean cloth. STEP 2: Place 500 ml rice wash to a jar and cover it with paper (use rubber band to tie the paper covering). Leave the jar in cool dark place for 5-7 days. STEP 3: After a week, when the bran has risen and it smells a little sour and forms 3 layers. This is indicating the rice wash is infected with various microbes. Take about 100 ml of clear water by straining again the mixture. STEP 4: Add 1 L of milk (to obtain 1:10 Water and Milk ratio). You may use skim or powdered milk, although fresh milk is the best. Cover the jar with paper same as before. Leave for 5-7 days in cool dark place.
  • 4. STEP 5: After about 1 week you’ll see curds (made of carbohydrate, protein, and fat) or the white part and yellow liquid or whey, enriched with lactic acid bacteria from the fermentation of the milk. Extract the whey by pouring through a strainer STEP 6: Add Molasses or brown sugar in equal part to the collected whey. This is to preserve the LABS at room temperature. So, if you have 1L of serum, add 1kilo sugar or 1L molasses. Otherwise store in fridge to keep. Then place it in clean container and label it accordingly USES AND APPLICATION OF LABS Based on our research we summarizes the different uses of LABS:  It can be used as alternative strategy to antibiotics growth promoters used in farm animals. Because continuous use of antibiotics may increase bacterial resistance, which can threaten the health of both animals and humans. Mix 2tbsp to 1L water, then add that mixture to animal’s water at 2tbsp/L (so the animal’s water contains little less than a quarter tsp/L of lacto serum). But this is very flexible. The Lacto serum is not harmful, so it’s just about adding enough to be effective, without wasting it.  In plants, spraying diluted solution of lactic acid bacteria serum (2tbsp. /1 liter of water) to the plant and soil helps plant growth and makes them healthier. As it is applied to the soil or the leaves, these beneficial bacteria aid in the decomposition process, thus allowing more food to be available and assimilated by the plant. And it is also known to produce enzymes and natural antibiotics aiding effective digestion and has antibacterial properties that serves as defense against fungi and viruses.  In Aquaculture, with regular addition of this beneficial organism to poor quality water (becauseof high amount of ammonia that pollutes water) ammonia problem is minimized and helps hasten or complete the denitrification or converting wastes into forms not harmful to fish. Add lacto at roughly 1L per 700m3 of fish-containing water.  Other applications: Used for its odor killing ability in animal wastes, clogged drains, foul odor of canals and addition to beneficial organism in composts.  RECCOMENDATION We recommend that: 1. Use LABS in morning, to avoid exposure to direct sunlight with UV that may kill or reduce their effectivity. 2. Avoid store milk that have antibiotic components. 3. During production and storage keep away the container from rainwater for this may cause contamination. Also keep it safe from wild animals. 4. Do not use plastic lids or covering that will prevent aeration. That’s why we use paper. 5. Use gloves and protective gears, aside from LAB it is possible that it contains harmfull organism that may enter your body through exposed body parts (ex. wounds)
  • 5. REFERENCES Ikeda, David M. et al. Sustainable Agriculture, “Farming: Lactic Acid Bacteria”. August 2013, CTHR. Minoa, Hawaii Gilliland, S. E., 1990. Health and nutritional benefits from lactic acid bacteria. FEMS Microbiol Rev. 1990 Sep;7(1-2):175-88. Lim, A. K., 2005. Handout on natural farming system and technology seminar. Davao: Tribal Mission Foundation International, Inc. Guolong Zhang, The Use of Lactic Acid Bacteria as a Probiotic in Swine Diets, Mar. 2015 URL: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4384071/ Rory Turnbull, DIY Lacto Bacillus Culture, Jan. 2013 URL: https://permies.com/t/25812/permaculture/DIY-Lacto-Bacillus-Culture Gil and Patrick, 2016 URL: http://theunconventionalfarmer.com/recipes/lactobacillus-serum/ Dugas, J., n.d. Lactic acid bacteria. URL: http://www.waksmanfoundation.org/labs/mbl/lactic.html.
  • 6. FISH AMINO ACID In “Natural Farming” natural materials are used instead of chemical inputs. One of the important tools used in Natural farming is Fish Amino Acid or FAA. These kind of bio fertilizers are easily made and prepared and help to reduce expenses of farmers. The Fish Amino Acid (FAA) is a liquid made from fish. FAA is of great value to both plants and microorganisms in their growth, because it contains various types of amino acids and abundant amount of nutrients. Studies have proved that Amino Acids can directly or indirectly influence the physiological activities of the plant. Amino Acids are also supplied to plant by incorporating them into the soil. It helps in improving the micro flora of the soil thereby facilitating the assimilation of nutrients. FAA is produced by fermenting fresh fish by-products (bones, head, skin, and other tankage parts) with brown sugar. Fresh fish scraps is collected and composted in a bucket added with molasses to the mixture to help build up microbes, and speed up decomposition. The brown matter molasses controls the odor and absorbs organic nitrogen from the fish. The bucket of fish is then made to rot for at least one week. The liquid is used to breed beneficial microbes and supply extra proteins to the emulsion. It provides an NPK ratio of 4-1-1 and is most often used as foliar feed to provide a quick nitrogen boost. MATERIALS NEEDED IN MAKING FISH AMINO ACID 1. 1 kilo of Fish trash (head, bone, intestines, scales, gills, etc) 2. 1 kilo of molasses 3. Jar or Contatiner 4. Paper and String/Rubber Band 5. Bottle for storage STEP BY STEP PROCEDURE 1. Collect a kilo of Fish Trash. And cut into pieces 2. Place the Fish Trash inside the Jar. 3. Put in a kilo of molasses in the Jar and mix. 4. Cover the Jar with paper and tie it with the string. 5. Place the jar in a cool dry place. Away from sunlight and rain. Leave it there for 20 days. 6. After 20 days, separate the liquid from the solids. The liquid has the Fish Amino Acids (FAA). 7. Transfer liquid into clean plastic bottles.
  • 7. USES AND RATES OF APPLICATION OF FERMENTED FRUIT JUICE PROPER USAGE: 1-2tsp per Liter of water  Plants- Aside from organic nitrogen sources, they also supply phosphorus, potassium, amino acids, proteins and trace elements or micro-nutrients that are really needed to provide deep nutrition to your soil community and plants. One of the benefits of fish emulsion is that they provide a slower release of nutrients into the soil without over- feeding all at once.  What more, using fish emulsion as our foliar spray helps us get rid of pests too. They hate the fishy smell, no matter how miniscule.  Soil – (1) Use as a soil drench as opposed to foliar spray. Whenever we drench the soil with fish, we build up the soil’s microbial activities, supplying lots of nitrogen to the roots and topsoil. (2) Inoculate compost to boost fungal population. (3) (4) Mix in water when watering plants, as a natural fish fertilizer and to enhance populations of micro- organisms in the soil  Animals- Mix with water for an effective protein/lacto boost for your animals. They'll love or hate it TIPS AND SUGGESTIONS  The bucket is stirred daily to get air into the mixture. Remember air allows for better decomposition and better aerobic microbial growth.  You will likely be able to get fish scraps or wastes in places where fish is cleaned prior selling or consumption. The fish section of the wet market will usually have traders removing fish parts like intestines, gills, liver, gall bladder, heart, fins, tail, scales and even bones. Typically, these are thrown away and so you could just ask them for free.  The pulp that will be left behind after extracting and collecting the fish emulsion is not wasted. It may be used as a good starter mix for the next batch of fish emulsion, because of its state of decomposition. It may also be used to feed a compost pile that can later be applied and mixed into your garden soil.  Never use directly to plant leaves or soil, you should dilute it with water. you should dilute fish amino acid with water.  It is not recommendable to use FAA during the period of reproductive growth, because it may induce over growth.  The optimum temperature for storage ranges between 23-250C andNo direct sunlight. Acool place is recommended.
  • 9. VERMITEA The presence of earthworms in soil ecosystems is an indicator of the well- being of a system. Earthworms have the unique ability to convert elements such as minerals, nutrients and microbes from the soil or composting systems into an excretion that contains the vastest amount of plant nutrients, microbes and growth elements that do exist. The excretion is called vermicast or vermicompost. Vermicompost is a compost that has been digested by worms (vermi) and does not need to be turned, because worms "turn" the organic matter in their digestive tract, eliminating work for gardeners. Vermicompost tea is a liquid derived from soaking and or brewing compost in water and is used as a foliar and soil application to plants. Vermicompost tea protected the leaves and stems of plants from the top down and not only from the soil. Direct application on the plant dramatically boosted its growth and vitality. The extract was rich in microbes, which helps restore plants after being exposed to pesticides and other environmental damage. It can contain the three basic plant nutrients: nitrogen in the form of nitrate or ammonium (N03 and NH4); phosphorus (P); and potassium (K). MATERIALS NEEDED IN MAKING VERMITEA 1. Large clean bucket, 2. High quality vermicompost / worm castings 3. Some type of permeable bag like nylon material (such as nylon stockings) for holding the compost; 4. Aged water – if you are using tap water you should let it sit for a day or two so as to remove the chlorine. Preferably, use some rainwater or pond water if you have some on hand. 5. A bucket STEP BY STEP PROCEDURE 1. Dissolve 2kg of molasses on the 200L water placed in a container 2. Put 10kg vermicast in a nylon stocking or similar permeable bag. . 3. Tie off nylon, and dip and submerged in water-molasses mixture. Cover the container to keep away from insect and animals. Place the brewing in a shaded area out of direct sunlight 4. After 3 days remove the vermicast sack. Mix the water mixture and this will be the Vermitea. Harvest and transfer to clean containers. USES AND RATES OF APPLICATION OF VERMITEA Use a ratio of 10 parts water to 1 part tea
  • 10.  Disease- For disease suppression on fruits, vegetables and ornamentals, applications can be made as often as every 5 to ten days.  As A Root Drench- Can be used unfiltered by applying directly to the soil area around a plant. The tea will step down into the root system. Root feeding is not affected by rainy weather.  Root Zone- Spray it over the root zone to feed the plant through the roots. I prefer to put compost tea in a watering can and water the leaves. A good amount drips off to enrich the soil. This is fast, easy, and effective.  Transplant Solution- Another great use for compost tea: use it as a transplant solution, to help your plants in transitioning to their new environment  Compost Pile- If you have any tea left over, consider putting it on your compost pile. This can help accelerate the microbial activity of your compost pile, which means quicker composting!  Plant. - Compost Tea can be applied about every two weeks to your garden plants once plants and transplants have become established. By established, we simply mean that they have been in the ground 7 to 10 days and are over the initial shock of transplant. TIPS AND SUGGESTIONS  Apply VCT early in the day, to allow the foliage to dry before night, which reduces the chance of foliar diseases  Use well-aged, finished compost  Unfinished compost may contain harmful pathogens and compost that is too old may be nutritionally deficient. COMPOST TEA and MANURE TEA ARE NOT THE SAME THING! Manure teas may be made in the same way but are not generally recommended as foliar sprays and are not as nutritionally well-balanced.)  If molasses are not readily available, you can use brown sugar.  Best if you put out the sack of vermicast 3 times a day.  Water your garden as you normally would  Water your houseplants  Use the worm compost tea to water seedlings or baby plants  Cover a whole plant with worm compost tea including the leaves. Many people believe that the beneficial microbes in worm tea help protect plants from diseases. A sprayer or spray bottle works well. Be sure to strain the worm compost tea before adding it to the spray bottle.  Serve worm compost tea at your next tea party. REFERENCES http://www.homecompostingmadeeasy.com/composttea.html http://www.healthy-vegetable-gardening.com/using-compost-tea.html http://farming.pinakbet.net/2013/02/how-to-make-vermi-tea/ http://www.extension.umn.edu/garden/yard-garden/soils/vermicompost-tea/ http://www.ijsrp.org/research-paper-0416/ijsrp-p5276.pdf
  • 11. ORIENTAL HERBAL NUTRIENT Oriental Herbal Nutrient (OHN), a fermented extractof herbs,is usedin Natural Farmingto provide plants and soil microorganisms with micro-nutrients, which may optimize their resilience to environmental stresses (wind, heat, drought, etc.). OHN is a mixture of edible, aromatic herbs extracted with alcohol and fermented with brown sugar. It is used to discourage the growth of anaerobic, potentially pathogenic microbesandencourage beneficialaerobicmicrobesin the soil and on plants.). These herbs are used in the creation of OHN. MATERIALS NEEDED IN INDIGENOUS MICRO ORGANISM 1. 3 or 5 of Plant material examples are: Garlic, Ginger, Onion, Siling Labuyo, Oregano etc. 2. Beer 3. Gin or CoconutVinegar 4. BrownSugar or Molasses 5. Jar or container 6. Paper and Rubber Band STEP BY STEP PROCEDURE 1. Chopequal amount of choice of herbs(Ex.1kg of Garlic, Gingerand Onion). Anycombinationof the above will work. Aneasierwayinsteadof choppingispressing,hammeringthemtobreakthe fibers. Place in the jar or container. Add beer to the level of the ingredients. Cover with paper and ensure with rubber band, keep in a dark, cool place. Leave for 12 hrs. 2. Add Molasses the amount of used individual herb (1kg molasses using above example) .Cover again with paper/rubber band and keep in a cool, dark place. Ferment for 7days. 3. AddGinorCoconutVinegar,equivalenttothe amountof Beerthatwasusedonday1.Coveragain and keep in a dark, cool place. 4. After10 days.Time to harvestthe OHN.Justfilteroutthe sludge.Thisisconsideredthe firstcycle 5. Repeat Step 2-4 for the 2nd cycle 6. Repeat Step 2-4 for the 3rd cycle USES AND RATES OF APPLICATION OF IMO PROPERUSAGE: 1-2tsp perLiterof water  PEST AND DISEASES o It's primarilyusedasanIPMinput.ItkillsPMand downymoldverywell,promotinggood colonization of microbes. o Apply as a foliar spray on plants to repel insects. Apply during late afternoon or early morning hours. o When plants have soft rot or anthracnose add water soluble calcium (WCA 1000) to this solution.
  • 12.  ANIMAL o OHN keeps our chickens healthy. Use it 3x a week to strengthen their immune system. Whenthe chickensare sick,theyare givenOHN for3daysstraight. Use 2Tablespoonsfor every liter of fresh water.  SOIL o Apply diluted OHN as a soil drench prior to planting to activate dormant soil microorganisms.  PEST AND DISEASES o It's primarilyusedasanIPMinput.ItkillsPMand downymoldverywell,promotinggood colonization of microbes. o Apply as a foliar spray on plants to repel insects. Apply during late afternoon or early morning hours. o When plants have soft rot or anthracnose add water soluble calcium (WCA 1000) to this solution. REFERENCES http://www.ctahr.hawaii.edu/oc/freepubs/pdf/SA-11.pdf http://www.pinoybisnes.com/agri-business/natural-farming-technology-and-its-techniques/ http://www.cgnfindia.com/ohn.htmlhttp://solraya.blogspot.com/2011/02/oriental-herbal-nutriets- ohn.html
  • 13. FERMENTED PLANT JUICE Fermented plant juice (FPJ) is derivedfrommixingthe youngshootsof the plants with molasses and/or crude sugar and fermented in one (1) week. The shoots of plantsare difficulttodissolve inwateror anykindof oil butitcanbe done withalittle amount of alcohol. The process of fermenting the soft part of the plants with molasses/crude sugar and with the presence of microorganisms will result to a small percentof alcoholwhichisresponsible in extractingthe juice from the young parts of the plants. FPJ is rich in micro and macro-nutrients. The primary elements that FPJ can provide are nitrogenandsome micro-nutrientslikecalcium,molybdenum, manganese,ironandcarbon.The juice also contains rich microorganisms which give strength to plants and animals MATERIALS NEEDED IN MAKING FERMENTED PLANT JUICE 1. The part of the plantsusedfor this fermentationisthe shootbecause it is thispart that storesa high percentage of nutrients coming from the soil and from the atmosphere. Moreover, plants usedforFPJare those thatare fastgrowingsuchthat,if you cut the shootnow,regenerationof a newpart will take effectinafewhours. We ourselvesuse: Kamote,Squash,Kangkong,Alugbati, Banana,Bamboo. You may use otherswithsame characteristicsandthe onesreadilyavailablein your area. 2. Youcan use eithercrude sugarormolassesorwhicheverisavailableandcanbe boughtatalower price. 3. You will need basin, ceramic pot or plastic pail, net bag or cloth bag, paper or cloth for cover, string, stone as weight, bolo, chopping board, marking pen and glass jars. STEP BY STEP PROCEDURE 1. Shake off dirt from the Plants but do not wash in water. Washing will remove useful microorganisms.If the ingredientsare too big,cut themto adequate sizes,about3 to 5 cm. This increases contact surface area and promotes osmotic pressure. 2. Measure the weight of the ingredient and the weight of brown sugar. Brown sugar should be between half of the weight of the ingredient. 3. Put the ingredientsandbrownsugarin a large wide containerandmix.Coverwithporouspaper and leave for 1 to 2 hours.
  • 14. 4. Put the mixture intothe jar.It shouldfill up¾of the jar. It is importantthat the jar is nottoo full or under full. The empty space is not empty. It is filledwith air, for optimum fermentation to occur. 5. Puton the cover and tie the jar. A coverisneededtopreventinsectsfromgettingintothe mixture. Paper is ideal because it lets the air in and out. 6. Remove the weight after 1 or 2 days. After the air has escaped, put back the cover again. 7. Putthe jarin a cool and shaded place.Donotopen,move orstirthe ingredientsduringthe process of fermentation. 8. After15days,strainthe contentsof the pail andtransferliquidtoacleancontainer. Younowhave Fermented Plant Juice which is a very good fertilizer. USES AND RATES OF APPLICATION OF FERMENTED PLANT JUICE  As seed treatment before sowing – soak the seeds in 0.2 % solution for 4 to 5 hours to facilitate germination and as a start-up solution to germinating seeds.  As a natural growthenhancer– FermentedPlantJuice made fromactivelygrowingplantpartsand fastgrowingplantsmaycontainnatural growth hormonesandmineralizednitrogenthatpromotes plantgrowth.Mix 1 teaspoonof FermentedPlantJuice perliterof waterandsprayonthe leavesor applydirectlyto the soil around the plantsfrom seedlingstage upto pre-floweringstage.Youcan applyweeklyordependingonplantvigor.Please note thatwith the use of FermentedPlantJuice, there is no overdose; you may use it liberally. However, the soil must be watered first before applying Fermented Plant Juice to avoid scorching of the roots.  Apply Fermented Plant Juice to the soil to serve as source of energyto accelerate activities of soil microorganism. This activity will make the nutrients available to the plants.  Give Fermented Plant Juice, as drink, to livestock at 1 tbsp/liter to increase microbial activities in gastrointestinal tracts. This would result to better absorption of nutrients.  Spray to animal beddings to hasten manure decomposition. TIPS AND SUGGESTIONS o The FPJenhancesgrowthandprovidesfoodforthe indigenousmicroorganisms(IMO). We addit to the drinkingwaterof our chickensandsprayit all aroundthe farm on the seedlings,vegetable plots and fruit trees. o The mostimportantrequirementwhenselectingplantsformakingFPJis to use the growingtips of plantspeciesthatare fastgrowers.Flowers,flowerbuds,andimmature fruitscanalsobe used. Hard orwoodyplantpartswill yieldlittleornoplantjuice.The plantsshouldbe vigorouslygrowing at the time of collection.Plantpartsshouldbe harvestedwhilethe plantsare inrespirationmode (before sunrise) and not in photosynthetic mode (during daylight), due to the effects these processes have on plant chemistry. o Polyethylene or glass products or clay jar may be used as a container. When usingglass bottles, brown glass containers must be preferred. o Store in a cool place. Select a shaded area where there is no direct sunlight and where the temperature does not fluctuate. Direct sunlight should be avoided.
  • 15. o The optimum temperature range is 1 to15ºC for storage (Use a Refrigerator if available) if you want to keep for one year. Otherwise one can use within 30 days store at room temperature. o WHEN NOT TO USE FPJ  Duringexcessivevegetative growthorovergrowthdue toprolongedrainsorcloudyweather.  FPJ made from the crop itself should not be used as this will promote further growth.  During acidic or nitrogen-excessive crop conditions, which create a pest-attractive environment.  Conditions of too much moisture or of bad ventilation, which promotes fungal growth. REFERENCES http://businessdiary.com.ph/3468/how-to-make-fermented-plant-juice-or-fpj/ http://www.cgnfindia.com/fpj.html http://www.freewebs.com/jumblebox/blogF/jumbleblog/fertilize2.html#.WDoLu9IrLcc http://www.agnet.org/library.php?func=view&id=20110804162833 http://solraya.blogspot.com/2010/09/fermented-plant-juice-fpj.html http://naturalfarminghawaii.net/wp-content/uploads/2013/09/Fermented-Plant-JuiceFPJ.pdf
  • 16. FERMENTED FRUIT JUICE Fermented fruit juice, also known as FFJ, is a partof the ‘Natural farmingmethod’developedby Dr. HanKyuChoof SouthKorea.Thisconcoctioncan be made at home from many common overripe sweetfruits.Itusesbrown(raw)sugartoextractthe phytochemicals in fruits. The fermenting process takes at least a week to complete. This liquid have nutritional activation enzymes and help to increase nutrients in the soil and therefore in the plants growing in it, especially potassium levels. It is commonly used as a foliar spray to enhance floweringandfruitingstage.Now,itisalsouse inanimal productionasitgivessupplemental nutrientsto animals. MATERIALS NEEDED IN MAKING FERMENTED FRUIT JUICE 1. Locallyproducedsweetripe fruitslike mango,banana, papaya,strawberryandchico;ripe squashfruit and matured carrot; and root crops particularly camote, cassava and gabi. Citrus fruits are not recommended.You can make Fermented FruitJuice fromsingle material or a combination of materials. The extract from the combination of banana, papaya, and squash have been proven to be effective in flower induction and fruit setting by many organic farmers. 2. Youcan use eithercrude sugarormolassesorwhicheverisavailableorcanbe purchasedatlowercost. 3. You will also need ceramic pots or plastic pail, basin, net bag or cloth bag, paper or cloth for cover, string, stone as weight, bolo, chopping board, marking pen, and glass jars for storage. STEP BY STEP PROCEDURE 1. Prepare the Ripe Fruit Meat by peeling the skin and removing the seed of the fruit. 2. Chop the Ripe Fruit Meat into little pieces. 3. Put chopped materials in the jar and add exact amount/weight of crude sugar or molasses, and then mix. You must make sure that all chopped materialsare coated with sugar or molasses so that the juice can be extracted easily. 4. Cover the jar with paper and tie it securely with the string. 5. Leave the jar in a cool and dry place for 7 days to ferment. Away from sunlight and rain. Make sure that the storage area is not infestedwithcockroachesormice,because theymightfeedon the mixture and contaminate the extract. In 7 days, plant juice is extractedand fermented. The fruitextractwill change itscolorfromyellow orangetobrown,andwillsmell sweetandalcoholic. 6. After 7 days, separate the liquid from the solids. The liquid is the Fermented Fruit Juice (FFJ).
  • 17. USES AND RATES OF APPLICATION OF FERMENTED FRUIT JUICE  Asflowerinducerandfruitsetter –FermentedFruitJuice madefromacombinationof ripe fruits of banana,papayaand squashhave beenprovenbymany organicfarmersto be effective when sprayedon the leavesatthe rate of 2 to 4 tbsp./gallonof waterat the onsetof floweringupto fruit setting. These ripe fruits contain phosphorous and potassium which are necessary during the flowering and fruit setting stage.  As soil microorganism activity accelerator – Fermented Fruit Juice is applied directly to the soil at the rate of 1tsp/literof water.The carbohydratesandsugar contentof Fermented FruitJuice serve as source of energy of soil microorganism, thereby, accelerating their activity.Increased microbial activities result to the availability of nutrients for plant’s uptake.  As spray to animal beddingstohastenmanure decomposition –FermentedFruitJuice contains beneficial microorganisms that help in the decomposition process.  As a nutritious drink – a 20% Fermented Fruit Juice solution makes an excellent drink for both human and livestock. TIPS AND SUGGESTIONS o Use any materials that are free from insect pests and diseases. o Avoid using fruits that were treated with Calcium Carbide (kalburo) or any other chemical treatments. o Do not use white sugar. o Youdo nothave tomix the brownsugarwithwatertomake itliquid.The risein temperaturedue to fermentation will melt the brown sugar. o If jars are too expensiveorare not readilyavailable inyourlocationyoucanuse plasticwater containers. o Always leave 1/3 of the bottle empty so the liquid can “breathe” o Don not tighten the bottle lid for 2 weeks after bottling to allow gasses to escape and avoid a sticky explosion o Solid materials from the fermentation process can be given to animals as feeds or to plants as compost o FFJ should have a pleasant smell and sweet and tangy taste o FFJ keeps for over a year or longer if refrigerated REFERENCES http://businessdiary.com.ph/3468/how-to-make-fermented-plant-juice-or-fpj/ http://www.cgnfindia.com/fpj.html http://jumblebox.webs.com/blogF/jumbleblog/fertilize3.html#.WDeAiLIrKM8 http://www.pinoybisnes.com/food-business-ideas/how-to-make-fermented-fruit-juice-for-natural- health/ http://homeguides.sfgate.com/make-fermented-fruit-plant-food-63700.html http://theunconventionalfarmer.com/recipes/bloom-fertilizer/
  • 18. INDIGENOUS MICRO ORGANISM IndigenousMicro-Organism(IMO) isone of the most basic and important input in natural farming. IMO are naturally occurring microbes from bacterial and fungal sources that have uniquelyevolvedtothrive inyourparticulararea and are collected and used to re-populate the soil's micro-sphere and the plant's surfaces - bringing them back into balance. This balance serves as the foundation for a healthy soil biology, productive nutrient recycling, and optimized plant growth/production. A large diversityof IMOcan be foundinforests,bamboo grovesandareaswiththickaccumulationofplant residues. Advantages of IMO are: (1) Organic & chemical-free Safe, (2) cheap and easy to use. (3) Nature & environmental friendly MATERIALS NEEDED IN INDIGENOUS MICRO ORGANISM 1. Container(bamboosplitopenorwoodenbox/plastictrayabout81/2”x11”x3”) 2. Manilapaper 3. String 4. 1 kg cookedcool rice 5. 1 kg molasses 6. Plasticbag 7. Plasticpail STEP BY STEP PROCEDURE 1. Weigh1 kg of cookedcool rice and place inside achosencontainer. 2. Coverthe containerwithcleanManilapaper;tie withstringthenwrapwithplasticbag. 3. After3 days checkif there are white moldsontopof the rice. If none yet,returnandkeepit there until there are enoughmolds. 4. Remove the moldyrice fromthe container,place inaplasticpail and add1 kg molasses. Be sure that the containerisonly70% full. 5. Coverthe pail witha cleansheetof paperand store ina drycool place,awayfromsunlight. Label accordingly. 6. After7 days,the mixture willyieldamudlike juice,youcanremove the liquid. 7. Label withthe name of the mixture,the startof the processand the date of harvest. 8. Strainand bottle.
  • 19. USES AND RATES OF APPLICATION OF IMO PROPERUSAGE: 1-2tsp perLiterof water  EnrichesSoil -Usingof IMO buildsupthe soil’slife anddiversity.Theiressential workistobreak downnutrientsintoformsthatare accessible toplantsandanimals.  Protectscrops fromdiseases- The more sterilizedyoursoil isthe fewermicroorganismsitwill have,thiswill invitesdiseasescausingthemtopropagate explosively.A webof livelyanddiverse microorganismsprovidesthe necessarychecksandbalancespreventingthisfromoccurring.  Plants- Applyasa foliarsprayor soil drench.Greatlyenhancesgrowthandhealthof plantsby establishingahealthypopulationof microbesinthe soil andonleaf surfaces.Transportsfoodto roots buildsahealthyecosystemfromthe groundup.Thisisan invaluable jobandthe greatest benefitof thisserum.Aidsdiseaseresistance –fightspathogens,occupiesspacesthatcould otherwise gotoharmful bacteria/molds.Aidcomposting –massivelyenhancescompost –there will be a whole separate postonthisconceptAidorganicfertilizer.Addtoyournutrient solution,microbesbreakdownorganicnutrientsintobio-availableformsthatplantscanutilize directly  Animals - Boostgrowthby enhancingdigestionandaidsdisease resistance.Fightthe bad bacteria.Usedto inoculate farmyard(sprayground) where animalsoccupytomaintainhealthy microbial system.Usedtocreate andmaintaina healthydeepbedlitterflooring.Itisthese micro-organismsthatwill helpbreaksdown fecal matterrapidlyleavingyouwithafertilizerrich material whilstensurenosmellsorflies