Successfully reported this slideshow.
We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. You can change your ad preferences anytime.

Industrial scale production of amino acid fertilizer from fish waste and under utilized fish

4,160 views

Published on

The full proceedings paper is at: www.extension.org/72823

With a dramatically increasing world population and a world catch of fish of more than 140 million tons per year, there is obviously an increased need to utilize our marine sources with more intelligence and foresight. Large amounts of protein-rich by-products from the seafood industry along with under-utilized fish are discarded or processed into fish meal and fertilizer. Novel processing methods are needed to convert seafood by-products into more profitable and marketable products. Proteins from fish processing by-products can be modified to improve their quality, functional characteristics and nutritional value by enzymatic and chemical hydrolysis. Protein from fish by-products and under-utilized species, are rich in amino acids and could be used as fertilizer.

Published in: Education
  • Be the first to comment

Industrial scale production of amino acid fertilizer from fish waste and under utilized fish

  1. 1. Mahmoudreza Ovissipour, Gleyn E. Bledsoe, Barbara Rasco School of Food Science: Washington State University, University of Idaho Waste To Worth 2015 Seattle WA March 30 - April 3, 2015
  2. 2. Collaborators 2 Tabarestan Science and Technology Incubator, Sari, Iran Caspian Zist Fanavaran Co. Iran Tabarestan Genetics and Biotechnology Research Institute, Sari, Iran Sari Agricultural Sciences and Natural University, Sari, Iran
  3. 3. Wheat Production State of Art in Iran  Wheat production in 2014 in Iran: 13.5 million tons  Iran is the 12th largest producer of wheat in the world  Average precipitation in Iran: 270 mm  Average precipitation in U.S.A: 715 mm, and in WA: 976 mm  Problem: The salt concentration is increasing in water and soil which are used for wheat 3
  4. 4. Underutilized Fish  Anchovy Sprat (Clupeonella engrauliformis) 12000 Mtons per year (from the Caspian Sea)  Main application is fish meal production  Seafood production in Iran: 562,595 Mtons 4 Viscera (12-18%): Fish meal, Food and Feed, Oil, Enzymes Fillet (40-60%): Food Bone (9-15%): Fish meal, Collagen, Gelatin Head (9-12%): Fish meal, Food, Oil Skin (1-3%): Fish meal, Collagen, Gelatin, Leather Fish Wastes Enzymatic Hydrolysis Chemical Hydrolysis Autolysis and Enzymatic Hydrolysis Autolysis
  5. 5. Project  Produce amino acid fertilizer from under-utilized fish and fish waste at an industrial scale  Desired Effect: Increasing the wheat production  Desired Effect: Increasing the wheat resistance to salinity 5
  6. 6. Amino Acid Fertilizer Production 6 Under-utilized fish or fish waste Grinding water (1:2 w/v) Heating at 50 °C Adding Alcalase (1% v/w) Enzyme Deactivation 90°C, 10 min Centrifugation or Decanting to Separate Liquid and Solid Phases Enzymatic Hydrolysis 5 h
  7. 7. The Effect of Fish Amino Acid Fertilizer (FAAF) on Wheat 7 Roshan Cultivar Growth Chlorophy ll Salinity Res. 20 days trial Amino- Hirkan ™ A comparison with 4 commercial amino acid fertilizers was conducted 40 and 80 mM Greenhouse conditions: Temperature: 26°C Relative Humidity: 40% Photoperiod: 16L:8D
  8. 8. Results 8
  9. 9. 9 Parameter F-1 F-2 F-3 F-4 Amino-Hyrkan Chlorophyll (mg/g wet weight) 3.86±0.02b 3.92±0.08b 3.88±0.1b 4.11±0.02a b 4.48±0.13a Catalase (control) (U/mg Protein) 0.777±0.058 0.821±0.121 0.685±0.072 0.831±0.114 0.725±0.031 Catalase (40 Mm) (U/mg Protein) 0.784±0.042 0.756±0.055 0.745±0.43 0.786±0.0 62 0.765±0.053 Catalase (80 Mm) (U/mg Protein) 0.715±0.076 0.794±0.087 0.720±0.038 0.740±0.0 67 0.688±0.083 Peroxidase (control) (U/mg Protein) 6.12±0.14bB 5.87±0.2bB 6.34±0.16bC 6.82±0.8b C 8.84±0.14aC Peroxidase (40 Mm) (U/mg Protein) 8.95±0.08b A 8.9±0.03bA 9.08±0.05bB 9.13±0.08b B 11.23±0.06aB Peroxidase (80 Mm) (U/mg Protein) 9.14±0.20bA 9.05±0.11bA 9.45±0.14bA 10.22±0.11b A 13.54±0.36aA Chlorophyll Production and Salinity Resistance The influence of four different commercial amino acid fertilizers and Amino-Hyrkan™ on Roshan wheat cultivar chlorophyll, and response to salinity 1 Values with different lower case letters in same column are significantly different (P < 0.05) 2 Values with different upper case letters in same row are significantly different (P < 0.05)
  10. 10. Growth Parameter F-1 F-2 F-3 F-4 Amino- Hyrkan Seedling Height (cm) 21.34±0.75b 21.08±1.11b 21.64±0.54b 21.52±0.35b 27.45±0.83a Seedling weight (g) 4.1±0.42b 4.02±0.23b 4.14±0.63b 4.23±0.81b 6.3±0.5a 10 The influence of four different commercial amino acid fertilizers and Amino-Hyrkan™ on Roshan wheat cultivar growth Values with different upper case letters in same row are significantly different (P < 0.05)
  11. 11. Growth Amino acid Quantity (g/100 g) F-1 F-2 F-3 F-4 Amino- Hyrkan™ Histidine 5.77 5.87 7.92 8.1 9.12 Isoleucine 5.26 5.16 6.14 5.54 7.21 Leucine 6.91 6.11 7.42 6.87 7.67 Lysine 0.97 0.67 1.27 2.65 3.82 Methionine 0.85 1.55 1.32 2.64 1.52 Phenyl alanine 2.41 2.41 3.57 3.83 3.76 Tyrosine 0.48 1.42 1.11 1.19 1.47 Threonine 4.87 3.64 4.87 4.3 5.1 Tryptophan _ _ _ _ _ Arginine 7.08 6.73 7.78 6.83 8.32 Valine 7.2 6.91 7.42 8.83 7.87 Aspartic acid 8.32 9.66 10.33 9.23 11.45 Glycine 3.1 2.88 4.18 6.08 4.58 Alanine 2.23 2.15 1.86 2.86 2.7 Glutamic acid 12.76 14.22 15.31 16.23 15.65 Serine 5.42 4.98 6.38 7.38 6.63 Total 73.63 74.36 86.88 92.56 96.87 11 Amino acid composition of four commercial amino acid fertilizer and Amino-Hyrkan
  12. 12. Commercialization Lab Scale Test Greenhouse Test Farm Test 12
  13. 13. Other Related Products from Fish Wastes 13 Algae Soda Peptone from Tuna Wastes Feeds
  14. 14. Continuing Efforts  Optimization of amino acid fertilizer composition based on application to specific crops  Determination of the effects of seafood-based amino acid fertilizer on nutritional value, and the disease resistance of plants  Amino acid fertilizer production from other sources such as tuna wastes, stickwater, restaurant wastes, and slaughter house by-products 14
  15. 15. Seafood Processing Waste
  16. 16. Flying Fish Heads 16
  17. 17. Simple Fish Compost 17
  18. 18. Thank You! Questions ? 18

×