BioCision provides products that enable consistent sample handling and processing through standardized cooling and freezing. Their CoolRack, CoolBox, and CoolCell products provide precise temperature control and uniform freezing profiles to reduce variability between trials. CoolRack modules rapidly cool or freeze samples in ice, dry ice, or liquid nitrogen and maintain highly consistent temperatures between wells. CoolBox allows ice-free transport and storage of samples. CoolCell provides a standardized and alcohol-free method for freezing cells that results in identical freezing profiles and high cell viability.
This document introduces several products from CoolProducts that aim to standardize sample handling and processing through consistent temperature control:
1) CoolRack is a modular system of tube and plate holders that can rapidly cool or freeze samples in a uniform and reproducible manner from -150°C to +200°C using various media like liquid nitrogen or dry ice.
2) CoolBox provides portable ice-free cooling or freezing of samples for up to 10 hours within 0.1°C accuracy across all wells.
3) CoolCell is an alcohol-free container that freezes cells in a highly reproducible manner with better viability compared to other methods.
Susan M. Smith Assistant: Jennifer L. Jones
Tel: 410-247-2500, x204 Tel: 410-247-2500, x205
Email: ssmith@biostorage.net Email: jjones@biostorage.net
Laboratory Director: Repository Manager:
Dr. John P. Williams Michael J. Wilson
Tel: 410-247-2500, x206 Tel: 410-247-2500, x207
Email: jwilliams@biostorage.net Email: mwilson@biostorage.net
Quality Assurance Manager: IT Manager:
Lisa A. Johnson, PhD Thomas E. Brown
Tel: 410
Biomatrica offers technology to stabilize biological samples like DNA, RNA, proteins, cells and assays at room temperature. Their core technology allows for more efficient handling, storage and shipping of samples without degradation. Their products include solutions to preserve and store genomic material, RNA, bacterial cells and assays at room temperature for long periods of time. Biomatrica's technology addresses a large market for sample storage and could be expanded to new areas like protein stabilization and diagnostics.
The document discusses the benefits of exercise for mental health. Regular physical activity can help reduce anxiety and depression and improve mood and cognitive functioning. Exercise causes chemical changes in the brain that may help protect against mental illness and improve symptoms.
This document introduces several products from CoolProducts that aim to standardize sample handling and processing through consistent temperature control:
1) CoolRack is a modular system of tube and plate holders that can rapidly cool or freeze samples in a uniform and reproducible manner from -150°C to +200°C using various media like liquid nitrogen or dry ice.
2) CoolBox provides portable ice-free cooling or freezing of samples for up to 10 hours within 0.1°C accuracy across all wells.
3) CoolCell is an alcohol-free container that freezes cells in a highly reproducible manner with better viability compared to other methods.
Susan M. Smith Assistant: Jennifer L. Jones
Tel: 410-247-2500, x204 Tel: 410-247-2500, x205
Email: ssmith@biostorage.net Email: jjones@biostorage.net
Laboratory Director: Repository Manager:
Dr. John P. Williams Michael J. Wilson
Tel: 410-247-2500, x206 Tel: 410-247-2500, x207
Email: jwilliams@biostorage.net Email: mwilson@biostorage.net
Quality Assurance Manager: IT Manager:
Lisa A. Johnson, PhD Thomas E. Brown
Tel: 410
Biomatrica offers technology to stabilize biological samples like DNA, RNA, proteins, cells and assays at room temperature. Their core technology allows for more efficient handling, storage and shipping of samples without degradation. Their products include solutions to preserve and store genomic material, RNA, bacterial cells and assays at room temperature for long periods of time. Biomatrica's technology addresses a large market for sample storage and could be expanded to new areas like protein stabilization and diagnostics.
The document discusses the benefits of exercise for mental health. Regular physical activity can help reduce anxiety and depression and improve mood and cognitive functioning. Exercise causes chemical changes in the brain that may help protect against mental illness and improve symptoms.
A telecom audit is an analysis of a company's telecommunications expenditures like local service, long distance, data, internet, wireless, and conferencing. The audit identifies billing errors and overcharges, which commonly amount to 15-25% of annual telecom spend. Conducting an audit can secure credits and refunds while reducing ongoing service costs. Tri-Solve is an expert auditing firm that identifies savings through audits without requiring changes to service providers. Their process involves analyzing bills at their office and presenting results with minimal client involvement.
This document provides the address of a residence designed by Michelle Kaufmann Design located in San Francisco, CA. The address given is 3457 Moore Drive in Los Angeles, CA. The document lists two addresses but does not provide any other details about the residence or design firm.
The document discusses In-Voice, a telecommunications solution provider that aims to simplify telecommunications and reduce costs for users. It offers various services and devices including callback services, softphone applications, global phone numbers and compatible mobile devices. In-Voice's services are designed to make communication affordable, flexible and convenient.
This document contains quotes from Stanford University praising the CoolCell cell freezing container. Stanford University found that the CoolCell provided slightly better cell viability (>90%) than their current containers and generates no isopropanol waste. Overall, the CoolCell has proven to set a new standard in cryopreservation according to Stanford University. The document also contains graphs showing that DNA exposed to ultraviolet light breaks down over time, while DNA protected by the Dark Reader maintains its supercoiled structure. The Dark Reader also blocks ambient light and ultraviolet light better than being in the dark.
Cyber bullying takes many forms such as harassment, threats, denigration, impersonation, and exclusion carried out online. Statistics show that over 40% of kids have experienced cyber bullying at least once with the majority not telling their parents. Both boys and girls can be cyber bullies with older teens being more likely. Teachers, parents, and teens all have roles to play in addressing cyber bullying through school policies, monitoring online activities, encouraging victims to speak up, and protecting personal information. The key is a preventative response through education and open communication.
The document appears to be contact information for an author or blogger named Toha MH. It includes their email address, blog site, and web site. It also includes links to additional writing samples and stories on other sites. The document is written in another language, but primarily serves to provide the author's online profiles and links to their published creative works.
The document discusses the many roles of a librarian in a vocational/technical school setting. It outlines that librarians must be flexible and think outside the box as their patrons are non-traditional library users. Librarians are encouraged to define their patron population's needs, which include students, teachers, curriculum materials, technology support, and public library resources. They should look at circulation statistics, class use, computer labs, and study spaces to identify how to best serve patrons' needs. The document provides examples of roles including curriculum specialist, technology specialist, public relations specialist, and more to engage patrons face-to-face, extend library hours, and develop collections to meet the school's needs.
Q-breaks offers a variety of exciting vacation packages around the world for families, couples, and groups seeking adventure and exploration. Their packages include activities like beach vacations, mountain retreats, cruises, tours, and more. With options in popular destinations and affordable prices, Q-breaks aims to make customers' dreams of traveling come true and change the way they holiday forever. Customers have flexibility to purchase multiple packages to suit different family members and travel needs.
This document provides information about vacation ownership plans with QVI Club that offer long-term affordable vacations for 5-30 years. Memberships include options for 1 or 2 week vacations annually or biennially for the membership period, allowing 60-15 weeks of vacations over 30 years depending on the plan. Details are provided on sample memberships, resort locations in Asia and Africa, transportation and amenities. Special short-term packages combining stays at QVI Club and XchangeWorld resorts are also described.
This document summarizes the findings of a study on audience constructions in 10 commercial broadcast stations in Switzerland. The study found that:
1) Most stations did not have a clear conception of their target audience and the conceptions that did exist were vague.
2) While stations used audience research, the findings did not often lead to revised audience conceptions.
3) Program preparation was guided more by economic conceptions of audiences rather than their actual needs.
4) The audience's point of view was not systematically incorporated into editorial feedback and reviews.
How transparency and accountability can make development work Twaweza
1. Transparency and accountability efforts in Tanzania helped improve several development issues by publicizing information and enabling public pressure.
2. Initiatives like investigative journalism on education funding, publicizing audit reports, and creating a water point feedback mechanism informed citizens and compelled action from leaders.
3. This led to outcomes like schools receiving delayed funds, increased oversight of public money, and a focus on unequal water access.
4. The document argues that development is improved when citizens can use information to know what's happening, connect with others, and exert pressure for more responsive leadership and service delivery.
BioCision is a life science research and development company that standardizes processes from lab to patient. Our award-winning products enable researchers, clinicians and manufacturers to protect temperature-sensitive therapeutics, biological samples and biomaterials. By addressing temperature stability, BioCision strives to improve the success of therapeutic discovery and enable effective care delivery.
Presented at ISBER 2015, Dr. Alex Esmon, Senior Product Manager at Thermo Fisher Scientific explains how to manage the cold chain to minimize pre-analytical variability. In his presentation, he discusses three scenarios: the importance of maintaining sample conditions, concerns during inventorying, and sample transport (including unit transfer and content transfer). To read more about biobanking best practices, check out our Inside Biobanking blog here: http://acceleratingscience.com/biobanking/
A telecom audit is an analysis of a company's telecommunications expenditures like local service, long distance, data, internet, wireless, and conferencing. The audit identifies billing errors and overcharges, which commonly amount to 15-25% of annual telecom spend. Conducting an audit can secure credits and refunds while reducing ongoing service costs. Tri-Solve is an expert auditing firm that identifies savings through audits without requiring changes to service providers. Their process involves analyzing bills at their office and presenting results with minimal client involvement.
This document provides the address of a residence designed by Michelle Kaufmann Design located in San Francisco, CA. The address given is 3457 Moore Drive in Los Angeles, CA. The document lists two addresses but does not provide any other details about the residence or design firm.
The document discusses In-Voice, a telecommunications solution provider that aims to simplify telecommunications and reduce costs for users. It offers various services and devices including callback services, softphone applications, global phone numbers and compatible mobile devices. In-Voice's services are designed to make communication affordable, flexible and convenient.
This document contains quotes from Stanford University praising the CoolCell cell freezing container. Stanford University found that the CoolCell provided slightly better cell viability (>90%) than their current containers and generates no isopropanol waste. Overall, the CoolCell has proven to set a new standard in cryopreservation according to Stanford University. The document also contains graphs showing that DNA exposed to ultraviolet light breaks down over time, while DNA protected by the Dark Reader maintains its supercoiled structure. The Dark Reader also blocks ambient light and ultraviolet light better than being in the dark.
Cyber bullying takes many forms such as harassment, threats, denigration, impersonation, and exclusion carried out online. Statistics show that over 40% of kids have experienced cyber bullying at least once with the majority not telling their parents. Both boys and girls can be cyber bullies with older teens being more likely. Teachers, parents, and teens all have roles to play in addressing cyber bullying through school policies, monitoring online activities, encouraging victims to speak up, and protecting personal information. The key is a preventative response through education and open communication.
The document appears to be contact information for an author or blogger named Toha MH. It includes their email address, blog site, and web site. It also includes links to additional writing samples and stories on other sites. The document is written in another language, but primarily serves to provide the author's online profiles and links to their published creative works.
The document discusses the many roles of a librarian in a vocational/technical school setting. It outlines that librarians must be flexible and think outside the box as their patrons are non-traditional library users. Librarians are encouraged to define their patron population's needs, which include students, teachers, curriculum materials, technology support, and public library resources. They should look at circulation statistics, class use, computer labs, and study spaces to identify how to best serve patrons' needs. The document provides examples of roles including curriculum specialist, technology specialist, public relations specialist, and more to engage patrons face-to-face, extend library hours, and develop collections to meet the school's needs.
Q-breaks offers a variety of exciting vacation packages around the world for families, couples, and groups seeking adventure and exploration. Their packages include activities like beach vacations, mountain retreats, cruises, tours, and more. With options in popular destinations and affordable prices, Q-breaks aims to make customers' dreams of traveling come true and change the way they holiday forever. Customers have flexibility to purchase multiple packages to suit different family members and travel needs.
This document provides information about vacation ownership plans with QVI Club that offer long-term affordable vacations for 5-30 years. Memberships include options for 1 or 2 week vacations annually or biennially for the membership period, allowing 60-15 weeks of vacations over 30 years depending on the plan. Details are provided on sample memberships, resort locations in Asia and Africa, transportation and amenities. Special short-term packages combining stays at QVI Club and XchangeWorld resorts are also described.
This document summarizes the findings of a study on audience constructions in 10 commercial broadcast stations in Switzerland. The study found that:
1) Most stations did not have a clear conception of their target audience and the conceptions that did exist were vague.
2) While stations used audience research, the findings did not often lead to revised audience conceptions.
3) Program preparation was guided more by economic conceptions of audiences rather than their actual needs.
4) The audience's point of view was not systematically incorporated into editorial feedback and reviews.
How transparency and accountability can make development work Twaweza
1. Transparency and accountability efforts in Tanzania helped improve several development issues by publicizing information and enabling public pressure.
2. Initiatives like investigative journalism on education funding, publicizing audit reports, and creating a water point feedback mechanism informed citizens and compelled action from leaders.
3. This led to outcomes like schools receiving delayed funds, increased oversight of public money, and a focus on unequal water access.
4. The document argues that development is improved when citizens can use information to know what's happening, connect with others, and exert pressure for more responsive leadership and service delivery.
BioCision is a life science research and development company that standardizes processes from lab to patient. Our award-winning products enable researchers, clinicians and manufacturers to protect temperature-sensitive therapeutics, biological samples and biomaterials. By addressing temperature stability, BioCision strives to improve the success of therapeutic discovery and enable effective care delivery.
Presented at ISBER 2015, Dr. Alex Esmon, Senior Product Manager at Thermo Fisher Scientific explains how to manage the cold chain to minimize pre-analytical variability. In his presentation, he discusses three scenarios: the importance of maintaining sample conditions, concerns during inventorying, and sample transport (including unit transfer and content transfer). To read more about biobanking best practices, check out our Inside Biobanking blog here: http://acceleratingscience.com/biobanking/
The document provides information about BenchTop "K" Series Freeze Dryers (Lyophilizers) manufactured by VirTis. Key details include:
- The freeze dryers are offered in a range of condenser temperatures from -55°C to -105°C and condenser sizes from 3 to 8 liters, allowing for processing of different sample sizes.
- They include a microprocessor controller for monitoring parameters, hot gas defrost for quick turnaround between cycles, and options for freezing and drying samples in the condenser.
- A variety of optional accessories are available to accommodate different sample containers and configurations.
The CoolCell is an alcohol-free cell freezing container that provides a more consistent freezing rate of -1°C/minute compared to traditional containers like Mr. Frosty that use alcohol. The CoolCell controls the freezing rate more consistently without any fluids or maintenance, saving costs compared to alcohol-based containers that require replacing the alcohol every 5 uses. Studies also show the CoolCell has better cell viability outcomes compared to containers like Mr. Frosty.
The document describes how to snap-freeze samples using a CoolRack and CoolBox or insulated ice pan. It explains that placing the CoolRack directly on dry ice allows samples to be snap-frozen without alcohol in about 7 minutes. Samples can be left in the CoolRack while on dry ice or transferred to storage. Using a CoolBox keeps the CoolRack frozen for around 10 hours with the lid closed and reduces dry ice needs. Proper safety precautions around dry ice are emphasized.
Leveraging New Refrigerant Technologies: Phase Change Materials in Cold Chain...Cryopak
The purpose of a temperature controlled package is to maintain an interior thermal environment sufficient to meet the product’s temperature requirements.
Refrigerant packs in combination with insulated containers make up the bulk of temperature controlled packages.
The most commonly employed refrigerant material is water often with some additives to alter its properties.
Cryopreservation is a process that preserves biological material such as cells, tissues, organs, and embryos at very low temperatures. It allows for long-term storage. Key aspects covered in the document include:
- A brief history of cryopreservation including early pioneers and discoveries.
- Cryoprotectants like glycerol and DMSO are used to prevent ice crystal formation and reduce cell damage during freezing and thawing.
- Different cryopreservation techniques exist like slow freezing, rapid freezing, and stepwise freezing which control ice formation.
- Cryopreserved materials can be stored long-term in liquid nitrogen at -196°C or other cryogenic temperatures where biological activity is effectively stopped
This document discusses several glycol products from Dow for various applications. Dowfrost is a food-grade propylene glycol used in food processing and solar/thermal storage. Dowfrost HD is an industrial-strength propylene glycol for hydronics and geothermal. Dowtherm SR-1 and 4000 are ethylene glycols for industrial cooling. Dowcal contains ethylene and propylene glycols and is aluminum safe. All products provide freeze protection down to -60°F and burst protection to -35°F. Chempoint is Dow's national distributor with warehouses around the US that provide glycol products, fluid analysis, and technical support. Glycol is an effective and affordable means of
Freezing food involves lowering the temperature so that water inside the food freezes into ice crystals. This immobilizes water and reduces water activity to preserve foods. There are different freezing methods like air blast, tunnel, or immersion freezing that allow control over crystal size. Smaller crystals from fast freezing cause less damage to cell structure and texture. Proper frozen storage and thawing are also important to maintain food quality.
Freezing has been successfully employed for the long-term preservation of many foods, providing a significantly extended shelf life.
The process involves lowering the product temperature generally to -18 °C or below.The extreme cold simply retards the growth of microorganisms and slows
down the chemical changes that affect quality or cause food to spoil.
During freezing the cellular solution present in the food matrix is cooled to its initial freezing point, and further cooling causes the water molecule to
separate, forming ice crystal.
The migration of water molecules during crystallization led to an increase in osmotic pressure, further enhancing the water permeability of the cell membranes. This transport of water molecules, if not controlled, can eventually affect the microstructure of the frozen produce.
The freezing process occurs in two successive steps, i.e,
” NUCLEATION” and “CRYSTAL GROWTH”.
This document provides an overview of the freeze drying (lyophilization) process. It discusses the three main steps: freezing, primary drying, and secondary drying. Freezing involves cooling the material below its freezing point. Primary drying occurs under vacuum where water sublimates from the frozen material. Secondary drying removes unfrozen water under higher temperature and lower pressure. The document also describes the components and working of freeze drying equipment, including the refrigeration system, vacuum system, product chamber, and condenser. Common excipients used in lyophilized formulations are discussed, such as bulking agents, stabilizers, and buffers.
How is Phase Change Material used for storing thermal energy. Thermal battery, store energy from ambient and use in air-conditioning, differential power tariffs. Reduce global warming, save energy, green technology
This document discusses two types of freezers used in fish processing - immersion freezers and spray freezers. Immersion freezers freeze fish by submerging them in a liquid refrigerant bath, allowing for efficient heat transfer. Spray freezers spray fish with a refrigerated liquid like liquid nitrogen, rapidly freezing the surface. Both methods freeze fish quickly while preventing freeze burn. Immersion freezing risks refrigerant penetrating the fish, while spray freezing has high operating costs due to liquid nitrogen usage. The document examines the working principles, advantages, and disadvantages of each freezer type.
The document discusses 10 considerations for validating an autoclave: 1) Choosing the right sterilization cycle; 2) Which load configurations to use; 3) Choosing the right control for liquid cycles; 4) Determining the most difficult items to sterilize and worst-case locations; 5) Issues with using wired thermocouples; 6) Wireless data loggers provide more accurate temperature monitoring; 7) Placement of biological and chemical indicators; 8) Conducting sterilization trials; 9) Evaluating sterilization results; and 10) Documenting the validation report.
There are two main methods of freezing food commercially - fast freezing at temperatures below -25°C which forms small ice crystals, and slow freezing above -24°C which forms larger crystals that can damage the food. Common freezing techniques include air blast, fluidized bed, plate or belt, immersion, and cryogenic freezing using liquid nitrogen or carbon dioxide. The freezing method chosen depends on factors like the food quality desired, type and shape of the food, package used, required flexibility, and costs. Equations can be used to estimate freezing times based on properties of the food and freezing conditions.
Simultaneous Heating and Cooling functionality for a Lunch BoxIRJET Journal
This document describes a proposed lunch box design that enables simultaneous heating and cooling functionality. It would contain two separate compartments - one that stays hot and one that stays cold. A thermoelectric cooler module placed between the compartments would allow heat to be pumped from the cold to the hot side, maintaining different temperatures in each. An Arduino, temperature sensor, DC-DC converter and other electronic components would control the thermoelectric cooler module to heat or cool food as needed over extended periods. The design aims to improve on traditional insulated containers by allowing addition of energy to prolong freshness of foods stored inside.
KOLD-DRAFT has been manufacturing commercial ice machines that produce the best ice in the world. KOLD-DRAFT's exclusive "upside-down" horizontal evaporator ice-making system produces pure, solid, sparkling ice that cools faster, melts slower, and doesn’t distort the taste of beverages.
This document discusses frozen sections and cryostats. Frozen sections are prepared without dehydration or embedding to enable rapid diagnosis within 10 minutes. They have applications in intraoperative diagnosis, enzyme histochemistry, immunohistochemistry, and other techniques. Tissue is frozen using liquid nitrogen or other cryogenic methods, turning water within the tissue to ice which acts as an embedding medium for sectioning. Cryostats maintain low temperatures, typically -20 to -30°C, for sectioning frozen tissue blocks. Optimal cutting temperatures vary by tissue type and whether the tissue is fixed.
Lab Armor Beads are metallic beads that can replace water in water baths, aluminum blocks in dry baths, and ice in ice buckets. The beads provide temperature control while keeping experiments dry and free from microbial contamination. They save time and money by eliminating the need to empty, clean, and refill traditional water baths. The beads also help laboratories become more organized and eco-friendly.
KOLD-DRAFT Ice Machines Produce Pure, Quality Cocktail Ice CubesKOLD DRAFT
KOLD-DRAFT Ice machines produce pure, clear and large 1" ice cubes essential for cocktails and fine beverages. World famous ice and ice machines, KOLD-DRAFT.
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Adhd Medication Shortage Uk - trinexpharmacy.comreignlana06
The UK is currently facing a Adhd Medication Shortage Uk, which has left many patients and their families grappling with uncertainty and frustration. ADHD, or Attention Deficit Hyperactivity Disorder, is a chronic condition that requires consistent medication to manage effectively. This shortage has highlighted the critical role these medications play in the daily lives of those affected by ADHD. Contact : +1 (747) 209 – 3649 E-mail : sales@trinexpharmacy.com
TEST BANK For Basic and Clinical Pharmacology, 14th Edition by Bertram G. Kat...rightmanforbloodline
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share - Lions, tigers, AI and health misinformation, oh my!.pptxTina Purnat
• Pitfalls and pivots needed to use AI effectively in public health
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A proprietary approach developed by bringing together the best of learning theories from Psychology, design principles from the world of visualization, and pedagogical methods from over a decade of training experience, that enables you to: Learn better, faster!
Local Advanced Lung Cancer: Artificial Intelligence, Synergetics, Complex Sys...Oleg Kshivets
Overall life span (LS) was 1671.7±1721.6 days and cumulative 5YS reached 62.4%, 10 years – 50.4%, 20 years – 44.6%. 94 LCP lived more than 5 years without cancer (LS=2958.6±1723.6 days), 22 – more than 10 years (LS=5571±1841.8 days). 67 LCP died because of LC (LS=471.9±344 days). AT significantly improved 5YS (68% vs. 53.7%) (P=0.028 by log-rank test). Cox modeling displayed that 5YS of LCP significantly depended on: N0-N12, T3-4, blood cell circuit, cell ratio factors (ratio between cancer cells-CC and blood cells subpopulations), LC cell dynamics, recalcification time, heparin tolerance, prothrombin index, protein, AT, procedure type (P=0.000-0.031). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and N0-12 (rank=1), thrombocytes/CC (rank=2), segmented neutrophils/CC (3), eosinophils/CC (4), erythrocytes/CC (5), healthy cells/CC (6), lymphocytes/CC (7), stick neutrophils/CC (8), leucocytes/CC (9), monocytes/CC (10). Correct prediction of 5YS was 100% by neural networks computing (error=0.000; area under ROC curve=1.0).
Here is the updated list of Top Best Ayurvedic medicine for Gas and Indigestion and those are Gas-O-Go Syp for Dyspepsia | Lavizyme Syrup for Acidity | Yumzyme Hepatoprotective Capsules etc
Muktapishti is a traditional Ayurvedic preparation made from Shoditha Mukta (Purified Pearl), is believed to help regulate thyroid function and reduce symptoms of hyperthyroidism due to its cooling and balancing properties. Clinical evidence on its efficacy remains limited, necessitating further research to validate its therapeutic benefits.
2. CoolProducts
CoolProducts enable biological sample consistency,
reproducibility and standardization – user to user, site to
site, trial to trial.
CoolRack® CoolBoxTM CoolCell®
Error-Free / Ice-Free / Alcohol-Free /
Temperature Control Sample Handling Cell Freezing
2
3. Problem: Lack of Standardization
“The lack of standardized, high-quality biospecimens has
been widely recognized as one of the most significant
roadblocks to the progress of cancer research.”
- NIH / NCI / OBBR
• Worldwide effort to develop new standards for collecting,
handling, and processing pre-analytical samples (blood,
tissue, tumor, other biological specimens or sample
materials)
• EU: http://www.devicelink.com/ivdt/archive/09/04/003.html
• NCI/NIH: http://biospecimens.cancer.gov/practices/default.asp
• NIH Office of Biorepositories and Biospecimen Research 2010 Symposium: http://
www.brnsymposium.com/meeting/brnsymposium/2010/
• Duke: http://web.genome.duke.edu/cores/biorepository/protocols/
• Molecular Epidemiology Workshop, Shanghai, 2008
3
4. Solution: CoolProducts
Reproducible and standardized cooling, (snap)freezing, thawing
CoolRack in
water bath
CoolRack
in dry ice
Five consecutive freeze/thaw cycles using a CoolRack module show
highly reproducible freezing and thawing profiles
Biotech International (November 2010)
4
5. How does it work?
• CoolRack modules are made of a novel thermo-
conductive alloy material
• Replaces “direct immersion” into temperature
sources such as ice, dry ice, LN2, water bath
• Thermo-conductivity is the transfer of heat from
higher temperature region to lower temperature
region
• CoolRack on Ice: Heat from the relatively warmer
CoolRack module is transferred to the ice (dry ice,
LN2) until equilibrium is reached
• CoolRack in Water Bath: Heat is transferred from
water bath toward relatively cooler CoolRack until
equilibrium is reached
• CoolRack novel alloy evenly distributes the
temperature across all wells providing very uniform
and consistent temperature to all samples
5
6. CoolRack
CoolRack
Error-Free /
Cooling, Freezing or
Thawing
6
7. Ice Cooling
“BioCision’s proprietary and patented portable bench top tools reduce
variability and ensure consistency in clinical sample handling. How
samples are handled prior to analysis is critical to the outcome of experiments in
basic and clinical research. "Biotech International” (November 2010)
Current method of cooling samples in ice. The CoolRack modules instantly introduce
random layout can result in mis-identification, organization and consistent temperature to all
errors in aliquoting and temperature variability. samples (+/- 0.1oC).
7
8. CoolRack
Versatile
• CoolRack rapidly adapts to any temperature
• from -150oC through 100oC+
• cooling (ice), snap-freezing (dry ice, LN2),
• thawing/incubating (water bath, heat block)
Uniform temperature
• +/-0.1oC temp uniformity to all wells
• standardize assay to assay, user to use, site to site
Patented novel alloy construction
• very rapid thermo-conductivity/adaptivity
• temperature assurance – no mess, no uncertainty
8
9. Modular
For a variety of biological specimens
CoolRack
tube holders
CoolSink
Plate holders
CoolTray
platforms
9
10. Versatile
For a variety of applications
-150oC -78oC 0o to 4oC 37oC +
Liquid Nitrogen Dry Ice Ice Water Bath
• Eliminate direct immersion • Replaces direct contact with • Gain organization & stability • Keeps tubes out of water –
into LN2 dry ice in ice reduced contamination, labels
• All tubes are upright • Eliminates ethanol bath on • Even temperature distribution stay dry
• Same freezing profile for all the bench – cost savings, to all samples even as ice melt • Consistent thawing profile for
samples reduced hazard & waste • Labels stay dry and legible for all samples
• ThermalTray provides • CoolRack PF provides equal better sample identification • All wells at identical
elevation of samples & or faster snap-freezing than • Reduced risk of sample temperature - eliminates edge
increased distance between ethanol slurry contamination effect on multi-well plates
hands and LN2 • Standardized freezing - • Rapidly goes from ambient to • Stable support of tubes &
• Rapidly adapts from ambient minimized standard deviation in <4oC in 90 seconds plates
(25oC) to LN2 temp (-150oC) in freeze profiles • Using ThermalTray, 10-15
12-14 minutes • Improved work flow hours of <4oC with one pan of
• Adapts to -78oC in 5-7 ice
minutes
BioCision’s tools are unique and replace very rudimentary methods of sample preservation
(freezing, snap-freezing) and handling (cooling, thawing)
10
11. CoolRack Performance
Cooling Rate - Ice
§ Ambient to < 4oC in 60-90 seconds
§ 4-6 hours of use when placing CoolRack directly on
ice
§ Up to 14 hours cooling with a single pan of ice with
ThermalTray
Cooling rate on ice
Snap-Freezing Rate – Dry Ice
§ Equal or better freeze rate than dry ice/
ethanol bath
§ No alcohol required – cost savings, reduced
hazard
§ Consistent freeze profile for all samples
Freeze rate on dry ice Standard deviation
11
12. CoolSink Performance
Ø Multi-well plates placed directly on ice for
cooling do not achieve critical < 4oC
temperature in any of the wells (Fig. 2, A)
Ø CoolSink modules evenly distribute the ice
temperature to all wells (Fig. 2, B and C)
• All wells are <4oC
• Curvature of the plate accounts for center
well variability (Fig.1)
Figure 1. Variance in height of undersurface of 96-well flat- Figure 2. Final equilibrium well temperature for a 96-well flat bottom plate
bottom plate. The x and y axes represent the well number in direct contact with (A) crushed ice, (B) CoolSink 96Fwith a dry interface,
and letter, and the z axis represents the relative deviation of and (C) CoolSink 96F with aqueous conductive medium in the interface.
the surface in units of 0.001 in. Data was collected using a Colors represent 0.5°C temperature intervals of the corresponding plate
ball dial indicator with a resolution of 0.0001 in. wells. The white cell represents the well that was fitted with the
thermocouple probe. Published in Biotechniques, November 2010
12
13. Kits and Accessories
• CoolBox
• Ice-free cooling container
• CoolSystem
• Include ThermalTray LP, CoolRack(s),
large ice pan
• Accessories
• Ice pans
• Rectangular (9L & 4L) and round (4L)
o
• Temperature strips (1-8 C)
• LCD display adheres to all thermo-conductive modules
• CoolMat histology cutting mat
• Protects surface of ThermalTray and scalpel blade
13
14. Customer Feedback - CoolRack
Steffan Vartanian - Genentech
Don’t you hate it when you spend all morning preparing RNA or harvesting lysates, only to
come back from lunch to find your precious samples drowning in a pool of melted ice? ……
But after using the Thermal Tray and CoolRack a few times, my soggy-sample days are
officially over! Not only do these tools keep your samples chilled for hours, but they also
keep everything organized, safe, and secure. BioCision's products are very well made, well
designed, and just plain look cool. Highly recommended!
Hans B - Perkin Elmer
We have been using the CoolRack for snap freezing our samples for the past weeks with
great success. Since we were on a tight schedule we performed some validation in house
and were very pleased with the results.
14
16. CoolBox
Consistent ice-free cooling and freezing
• cartridges provide up to 10 hours of ice-free cooling or freezing
• continuous +/- 0.1oC consistency to all wells
Versatile
• five temperature options using cartridges,
dry ice or LN2
• portable
• accommodates variety of tubes & plates
Highly recommended for
• PCR and RNA work, cell culture, cooling in biosafety cabinet, GMP/GLP
suites and vivarium, bio-hazardous containment, short transport
16
17. CoolBox 30 & CoolBox Microplate
CoolBox Microplate System
• Ice-free SBS-format plate & tube cooling &
freezing
o
• Includes CoolBox, lid, blue +2 C cartridge
• Accommodates all SBS format modules -
CoolSinks, CoolRack PCRs, CoolRack 1mlx96
• Sold separately OR with CoolRack/CoolSink
CoolBox 30 System
• Ice-free tube cooling / freezing
o
• Includes CoolBox, lid, blue +2 C cartridge, red
stage, purple insulator pad
• Accommodates all 30-well CoolRacks
• Sold separately OR with CoolRack
17
18. CoolBox Performance
mmmm
-25oC with thermal regulator
pad placed between CoolRack
and dry ice
Snap-freezing at -78oC with
CoolRack directly on 200cc dry
ice
NOTE: Performance may vary depending on ambient
conditions, starting temperature and other specific set-up
criteria. See instructions for complete information.
18
19. CoolBox Advantage
CoolBox Gel-Filled Container
Well-to-well Temperature Variance: Well-to-well Temperature Variance:
+/-0.1oC Up to 4oC
Cooling Media: Cooling Media:
LN2, Dry ice, Wet ice, Cartridge Gel
Temperature range: Temperature range:
-150oC through +150oC Fixed at -20oC or 0oC
Tube & Plate Formats: Tube & Plate Formats:
0.5ml conical, 1.5ml conical, 1.5-2.0ml 0.5ml conical, 1.5ml conical, PCR tubes
cylindrical, 12.5mm dia cryos, mass
spec tubes, PCR 96- and 384-well
plates, 6-, 12-, 24-, 48-, 96-well plates
Cooling Duration: Cooling Duration:
10h (2ºC), 6h (-12ºC), 5h (-25ºC), 8h (0ºC), 3h (-21ºC)
6h (-78ºC)
Down Time: Down-Time:
None – switch cartridge with another 4-6 hours to re-freeze gel-filled tube rack
frozen one from the freezer or replenish
dry ice, LN2
CoolBox provides temperature uniformity and versatility
19
20. Customer Feedback - CoolBox
Fraser Moss Ph.D. - Case Western Reserve University, Dept. of Physiology and
Biophysics, President Scientist Solution
BioCision has now eliminated the ice factor and have the CoolBox in which you can keep the
96 well CoolRack…..I'm using it to keep my RNA cold for oocyte injections right now. I'll be
using it to assemble my PCR reactions in the not to distant future. This has been a great new
little toy to have on the bench recently because our departmental ice machine broke in April
and still is not fixed!
Laura Matthews - Sarah Cannon Research Institute (SCRI)
I am writing regarding the demo CoolRack and CoolBox that we have been testing. They are
both great products. The CoolRacks have eliminated the mess of mushy cardboard freezer
boxes. The samples also freeze much faster in the CoolRacks. The CoolBox was especially
helpful in the transport of fresh biopsies on dry ice from one location to another
20
22. CoolCell
Highest reproducibility
• -1oC/minute freeze rate
• Every freeze run is exactly the same
• Identical freezing profiles every time
Highest cell viability
• Cell lines, PBMC, primary cells, stem cells, yeast
Alcohol-free
• No alcohol – no fluids
• No on-going alcohol expense or waste
• No maintenance
• No variability due to changes in alcohol
22
23. CoolCell
Eliminates alcohol-induced freezing variation
• Many researchers currently use alcohol-filled containers to
achieve a -1oC/minute freeze rate when freezing cells in
-80oC freezer
Alcohol-filled container
• The alcohol must be replaced every 5 uses because the
alcohol changes each time it’s frozen
• Alcohol absorbs moisture and evaporates
• CoolCell uses no alcohol (no fluids)
• Nothing changes
• Identical cell freezing results every time
23
24. Reproducible Results
Five consecutive freeze cycles in CoolCell show fusion
time and cooling profiles are identical every time
24
25. High Cell Viability
In primary human cells, CoolCell significantly
outperforms
25
26. CoolSystemTM CRYO
• Kit for ice-free vial preparation in a laminar flow hood & alcohol-free cell
freezing in a -80oC freezer
• CoolBox CFT provides ice-free 0-4oC work station for 30 cryo vials and
allows one-handed vial opening and closing
• CoolCell provides alcohol-free and standardized cell freezing
CoolSystem CRYO (#BCS-142) includes CoolBox CFT30 System and CoolCell
26
27. CoolCell Advantage
CoolCell Alcohol-Filled Container
Very consistent -1oC/min rate Inconsistent freeze rate
• All vials have uniform freeze rate • Each run is different due to alcohol
every time degradation
• Radially symmetric – design enables • Two circles of wells – different freeze rates
consistency for each circle
No alcohol Requires 250ml alcohol
• No hazardous fluids • Replace alcohol every 5 uses (250ml)
• Solid core and insulation design • Keep track of # of uses
• No pre-cooling required • Alcohol degrades slightly each time it s
used
• Pre-cool alcohol in refrigerator
Cost savings ~$338/year maintenance per unit
• No maintenance cost • Change alcohol weekly (~$6/wk)
• Pays for itself quickly • Dispose haz waste (~$0.75/wk)
• 10 units = $3,380 to use EVERY YEAR
Lid opens easily Screw cap very difficult to remove when
• Not cold to the touch when removing frozen
from the -80C freezer • Protective glove required when handling
frozen unit
Better cell viability Good cell viability
Ready to use again in 5 minutes Takes >1 hour for device to return to
room temp for re-use
Low impact on freezer Large thermal mass impacts local
• 1/3 the heat impact on freezer freezer area
compared to alcohol-filled units • Large heat capacity removed from alcohol
impacts nearby samples
27
28. Customer Feedback - CoolCell
Rohit G. - Stanford University
We run a registry, in which large amounts of PBMCs are processed for long-term
cyropreservation.………Overall, the CoolCell has proven to us to set a new bar in
cryopreservation.
Srujan P. - Merck Pharmaceutical, Vaccine Manufacturing
I have already purchased 21 CoolCells, I have obtained a CoolBox, and I recently ordered a
96well flat bottom CoolCell system. All these will be used for our assays…. I already passed
on my evaluation of the cool cell and the other groups are pretty excited and are going to use
them.
Sam Knight - Ceramisphere (Australia)
Purely altruistically, I wanted to mention that one big advantage of the CoolCell is that you can
use dry ice to do your freezing if you don't have a -80. I spent nearly a year getting terrible
viability-recovery on cell freezing with dry ice (in an alcohol device) followed by storage in LN2,
and this on HeLa cells which aren't exactly hard to store. I then bought a CoolCell and now I
can just use standard cryo-preservation techniques (DMSO, 20% NBS) and get nearly 100%
viability on thawing.
28
30. Standardizing Clinical Sample Handling
• Clinical sample handling has become increasingly important
• growing number of multi-national clinical trials
• increasing use of biomarkers
• the move toward personalized medicine.
• BioCision products address common and often overlooked
inconsistent procedures in every laboratory
• Non-standardized methods of cooling/freezing/thawing
• Consistency. Reproducibility. Standardization.
• Test to test
• Researcher to researcher
• Lab to lab
• Site to site
32. Genentech Amgen Pfizer Roche Harvard MIT
CalTech UCSF Glaxo Bristol Myers Squibb Novartis
Translational Genomics Gilead Sciences Merck
MedImmune ATCC Genpharm Elan Pharmaceuticals
Centocor Boehringer Ingelheim Oncomed NIH/NCI
Takeda Pharmaceuticals CDC Covance XOMA
Thank you
Quest Diagnostics Otsuka America Pharmaceuticals
Salk Institute Burnham Institute Scripps Institute
Stanford University Yale Genzyme EMD Serono
Shire HGT Alnylam Wyeth Cell-Systems
Jackson Labs Beth Israel Deaconess Hospital HHMI
Whitehead Institute Harvard Systems Biology
Exelixis Charles River Labs Sanofi Pasteur Penn State
Lawrence Berkeley Lab Batelle UC Berkeley
Cedars Sinai Hospital Cleveland Clinic IDEXX Biogen
Perkin Elmer Genetics Blood Systems Research Inst.
Copyright 2011. Patents pending. All rights reserved.