The CoolCell is an alcohol-free cell freezing container that provides a more consistent freezing rate of -1°C/minute compared to traditional containers like Mr. Frosty that use alcohol. The CoolCell controls the freezing rate more consistently without any fluids or maintenance, saving costs compared to alcohol-based containers that require replacing the alcohol every 5 uses. Studies also show the CoolCell has better cell viability outcomes compared to containers like Mr. Frosty.
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Karthik Muralidharan on research on achieving universal quality primary educa...Twaweza
A presentation by Prof. Karthik Muralidharan on research on achieving universal quality primary education in India. This was presented at the Commission for Science and Technology (COSTECH) in Dar es Salaam, Tanzania, on June 19, 2014, to an audience of researchers.
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BioCision is a life science research and development company that standardizes processes from lab to patient. Our award-winning products enable researchers, clinicians and manufacturers to protect temperature-sensitive therapeutics, biological samples and biomaterials. By addressing temperature stability, BioCision strives to improve the success of therapeutic discovery and enable effective care delivery.
Presentation by Rachel Steinacher, on IPA and RCTsTwaweza
A presentation by Rachel Steinacher, Research Manager for IPA-Kenya (Innovations for Poverty Action), on IPA and RCTsThis was presented at the Commission for Science and Technology (COSTECH) in Dar es Salaam, Tanzania, on June 19, 2014, to an audience of researchers.
BioCision is a life science research and development company that standardizes processes from lab to patient. Our award-winning products enable researchers, clinicians and manufacturers to protect temperature-sensitive therapeutics, biological samples and biomaterials. By addressing temperature stability, BioCision strives to improve the success of therapeutic discovery and enable effective care delivery.
If you're a wine lover, then be sure to check out this presentation about how thermoelectric wine coolers work! We'll walk you through the basic technology, and help you decide if one of these appliances is the right choice for preserving your wine collection.
KOLD-DRAFT has been manufacturing commercial ice machines that produce the best ice in the world. KOLD-DRAFT's exclusive "upside-down" horizontal evaporator ice-making system produces pure, solid, sparkling ice that cools faster, melts slower, and doesn’t distort the taste of beverages.
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These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
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Microteaching is a unique model of practice teaching. It is a viable instrument for the. desired change in the teaching behavior or the behavior potential which, in specified types of real. classroom situations, tends to facilitate the achievement of specified types of objectives.
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This includes all relevant anatomy and clinical tests compiled from standard textbooks, Campbell,netter etc..It is comprehensive and best suited for orthopaedicians and orthopaedic residents.
Adv. biopharm. APPLICATION OF PHARMACOKINETICS : TARGETED DRUG DELIVERY SYSTEMSAkankshaAshtankar
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ADVANCED BIOPHARMACEUTICS & PHARMACOKINETICS : UNIT 5
APPLICATION OF PHARMACOKINETICS : TARGETED DRUG DELIVERY SYSTEMS By - AKANKSHA ASHTANKAR
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ASA GUIDELINE
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ABDOMINAL TRAUMA in pediatrics part one.drhasanrajab
Abdominal trauma in pediatrics refers to injuries or damage to the abdominal organs in children. It can occur due to various causes such as falls, motor vehicle accidents, sports-related injuries, and physical abuse. Children are more vulnerable to abdominal trauma due to their unique anatomical and physiological characteristics. Signs and symptoms include abdominal pain, tenderness, distension, vomiting, and signs of shock. Diagnosis involves physical examination, imaging studies, and laboratory tests. Management depends on the severity and may involve conservative treatment or surgical intervention. Prevention is crucial in reducing the incidence of abdominal trauma in children.
2. CoolCell
An alcohol-free cell freezing
container
CoolCell
Replaces “Mr. Frosty”
An alcohol-filled freezing container
Mr. Frosty
2
3. Background
Most cell culture researchers freeze cells in a
o
-80 C freezer prior to storing in liquid nitrogen
o
They need a slow freeze rate of -1 C/minute
“Mr. Frosty” is commonly used now
It uses 100% iso-propylalcohol (IPA) to control the freezing
o
rate to approximately -1 C/minute
“CoolCell” controls the freezing rate MORE
CONSISTENTLY
with no alcohol – no fluids – no maintenance – no cost
3
4. “Mr. Frosty”
The alcohol in “Mr. Frosty” must be replaced
every 5 uses in order to keep the freeze rate
o
near the desired -1 C/minute. This means…..
you have to keep track of how many times you use it (hassle!)
you have to purchase alcohol (expense!)
you have to dispose used alcohol (hassle, smell & expense!)
More importantly, it also means that the alcohol
level changes every time it is used
“Mr. Frosty” results in different freezing profile if not maintained
properly
It is a heat sucker “Caution” can ruin your samples/probes in
freezer
4
5. CoolCell
o
CoolCell delivers a very consistent -1 C/minute
freeze rate without alcohol
Freezing profiles are extremely consistent every time
Less variability and high repeatability in your research
Fusion time and cooling profiles are
highly reproducible from run to run
5
6. CoolCell
CoolCell outperforms “Mr. Frosty” in cell viability
Researchers care most about cell viability (i.e. how many cells
are still viable after they are thawed out at a later date)
In primary human cells, CoolCell significantly outperforms
6
7. Additional CoolCell Advantages
No on-going cost – COST SAVINGS!
“Mr. Frosty” costs approx. $350/year for alcohol purchase & disposal
Lid is easy to open – even when frozen
“Mr. Frosty” screw cap is very difficult to open when frozen
Not cold to the touch and not “frosty” when frozen
“Mr. Frosty” requires special gloves to handle and is very slippery due to frost/ice
on exterior
Ready to use again after only 5 minutes
“Mr. Frosty” requires about 1.5 hours for alcohol to thaw before it can be used
again
7
8. CoolCell vs “Mr. Frosty”
CoolCell “Mr. Frosty”
No alcohol Requires 250ml alcohol
• No hazardous fluids • Replace alcohol every 5 uses (250ml)
• Solid core and insulation design • Keep track of # of uses
• No pre-cooling required • Alcohol degrades slightly each time it’s used
• Pre-cool alcohol in refrigerator
Very consistent -1oC/min rate Inconsistent freeze rate
• All vials have uniform freeze rate every time • Each run is slightly different due to alcohol
• Radially symmetric – design enables consistency degradation
• Two circles of wells – different freeze rates for each
circle
Cost savings ~$338/year maintenance per unit
• No maintenance cost • Change alcohol weekly (~$6/wk)
• Pays for itself quickly • Dispose haz waste (~$0.75/wk)
• 10 units = $3,380 to use EVERY YEAR
Lid opens easily Screw cap very difficult to remove when frozen
• Not cold to the touch when removing from the -80C • Protective gloves required when handling frozen unit
freezer
Better cell viability Good cell viability
Ready to use again in 5 minutes Takes >1 hour for device to return to room
temp for re-use
Low impact on freezer Large thermal mass impacts local freezer area
• 1/3 the heat impact on freezer compared to alcohol- • Large heat capacity removed from alcohol impacts
filled units nearby samples
8