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20100426 Bio Cision Product Overview(3)
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- 2. CoolProducts CoolRackTM CoolBoxTM CoolCellTM Modular thermo- Ice-free cooling and Alcohol-free cell conductive tube and freezing freezing container plate holders Modular tools for cooling, (snap)freezing and thawing biomedical samples 2
- 3. Does this lookfamiliar? Most researchers still entrust their precious samples to this type of cooling method 3
- 4. Here’s the samebucket again…. But in this one, the samples are safely placed in one of our CoolRack modules 4
- 5. Why are CoolProductsnecessary? Worldwide effort to develop new standards for collecting, handling, and processing pre-analytical samples (blood, tissue, tumor, other biological specimens or sample materials) EU: http://www.devicelink.com/ivdt/archive/09/04/003.html NCI/NIH: http://biospecimens.cancer.gov/practices/default.asp “The lack of standardized, high-quality biospecimens has been widely recognized as one of the most significant roadblocks to the progress of cancer research.” NIH Office of Biorepositories and Biospecimen Research 2010 Symposium: http://www.brnsymposium.com/meeting/brnsymposium/2010/ Duke: http://web.genome.duke.edu/cores/biorepository/protocols/ Molecular Epidemiology Workshop, Shanghai, 2008 5
- 6. It’s all aboutthe curves…. 6
- 7. ….the temperature curves Each sample goes through the 0oC transition the same way. Cool, freeze and thaw - identical each and every time Consistent curves mean temperature control and experimental repeatability…..INSTANT STANDARDIZATION 7
- 8. CoolProducts CoolRackTM CoolBoxTM CoolCellTM Modular thermo- Ice-free cooling and Alcohol-free cell conductive tube and freezing freezing container plate holders 8
- 9. CoolRack Versatility - rapidly adaptsto any temperature - can be used for a wide temperature range - from -150oC through +200oC Consistency & Standardization - uniform temperature profiles for all samples - inexpensive, comprehensive modular solution -CoolRacks, CoolSinks, CoolBox, CoolTrays - reduced user error - standardize - assay to assay, user to user, lab to lab, site to site Organization - indexed & organized sample management - labels stay dry & legible - minimize risk of contamination Better sample organization Patented novel alloy construction & temperature consistency - very rapid thermo-conductivity/adaptivity 9
- 10. CoolRack Tube holders - somewith profile-fit Plate holders - multi-well & PCR bbbbbb Platforms - rectangular & round Modular system is customizable, scalable and versatile 10
- 11. CoolRack -150oC -78oC 0o to 4oC 37oC + Liquid Nitrogen Dry Ice Ice Water Bath • Eliminate direct immersion • Replaces direct contact with • Gain organization & stability • Keeps tubes out of water – into LN2 dry ice in ice reduced contamination, labels • All tubes are upright • Eliminates ethanol bath on • Even temperature distribution stay dry • Same freezing profile for all the bench – cost savings, to all samples even as ice melt • Consistent thawing profile for samples reduced hazard & waste • Labels stay dry and legible for all samples • ThermalTray provides • CoolRack PF provides equal better sample identification • All wells at identical elevation of samples & or faster snap-freezing than • Reduced risk of sample temperature - eliminates “edge increased distance between ethanol slurry contamination effect” on multi-well plates hands and LN2 • Standardized freezing - • Rapidly goes from ambient to • Stable support of tubes & • Rapidly adapts from ambient minimized standard deviation in <4oC in 90 seconds plates (25oC) to LN2 temp (-150oC) in freeze profiles •Using ThermalTray, 10-15 12-14 minutes • Improved work flow hours of <4oC with one pan of • Adapts to -78oC in 5-7 ice minutes Temperature control without directly immersing samples 11
- 12. CoolRack Cooling - Ice Ambient to < 4oC in 60-90 seconds 4-6 hours of use when placing CoolRack directly on ice Up to 14 hours cooling with a single pan of ice with ThermalTray Cooling rate on ice Snap-Freezing – Dry Ice Equal or better freeze rate than dry ice/ethanol bath No alcohol required – cost savings, reduced hazard Consistent freeze profile for all samples Freeze rate on dry ice Standard deviation 12
- 13. CoolProducts CoolRackTM CoolBoxTM CoolCellTM Modular thermo- Ice-free cooling and Alcohol-free cell conductive tube and freezing freezing container plate holders 13
- 14. CoolBox Ice-free cooling andfreezing - 4 hrs w/o lid, 10 hrs with lid (+2oC) - 3 hrs w/o lid, 6 hrs with lid (-12oC) Versatile - five temperature options in one box using cartridges, dry ice or LN2 - portable - accommodates a variety of tubes & plates Consistent temperature +2oC - all wells within 0.1oC regardless of position even as cartridges thaw Highly recommended - for highly-sensitive applications such as PCR and RNA, use in the hood, cleanroom, vivarium, short transporting, bio-hazardous containment Portable ice-free cooling or freezing 14
- 15. CoolBox -25oC with thermal regulator pad placed between CoolRack and dry ice Snap-freezing at -78oC with CoolRack directly on 200cc dry ice 15
- 16. CoolBox CoolBox Gel-Filled Container Temperature range: Temperature range: -150oC through +150oC Fixed at -20oC or 0oC Cooling Media: Cooling Media: LN2, Dry ice, Wet ice, Cartridge Gel Well-to-well Temperature Variance: Well-to-well Temperature Variance: 0.1oC Up to 4oC Tube & Plate Formats: Tube & Plate Formats: 0.5ml conical, 1.5ml conical, 1.5-2.0ml 0.5ml conical, 1.5ml conical, PCR tubes cylindrical, 12.5mm dia cryos, mass spec tubes, PCR 96- and 384-well plates, 6-, 12-, 24-, 48-, 96-well plates Cooling Duration: Cooling Duration: 10h (2ºC), 6h (-12ºC), 5h (-25ºC), 8h (0ºC), 3h (-21ºC) 6h (-78ºC) Down Time: Down-Time: None – switch cartridge with another 4-6 hours to re-freeze gel-filled tube rack frozen one from the freezer or replenish dry ice, LN2 CoolBox is more versatile, more consistent 16
- 17. CoolProducts CoolRackTM CoolBoxTM CoolCellTM Modular thermo- Ice-free cooling and Alcohol-free cell conductive tube and freezing freezing container plate holders 17
- 18. CoolCell Alcohol-free cellfreezing No hassle No on-going expense No variability High reproducibility Better cell viability Faster work flow CryoPrep System everything you need for cell prep in the hood – CoolRack CFT, CoolBox, CoolCell 18
- 19. It’s all aboutthe curves….. Fusion time and cooling profiles are highly reproducible from run to run 19
- 20. Better cell viability In primary human cells, CoolCell significantly outperforms 20
- 21. CoolCell CoolCell Alcohol-Filled Container No alcohol Requires 250ml alcohol •No hazardous fluids •Replace alcohol every 5 uses (250ml) •Solid core and insulation design •Keep track of # of uses •No pre-cooling required •Alcohol degrades slightly each time it’s used •Pre-cool alcohol in refrigerator Very consistent -1oC/min rate Inconsistent freeze rate •All vials have uniform freeze rate every •Each run is slightly different due to alcohol time degradation •Radially symmetric – design enables •Two circles of wells – different freeze rates for consistency each circle Cost savings ~$338/year maintenance per unit •No maintenance cost •Change alcohol weekly (~$6/wk) •Pays for itself quickly •Dispose haz waste (~$0.75/wk) •10 units = $3,380 to use EVERY YEAR Lid opens easily Screw cap very difficult to remove when •Not cold to the touch when removing frozen from the -80C freezer •Protective glove required when handling frozen unit Better cell viability Good cell viability Ready to use again in 5 minutes Takes >1 hour for device to return to room temp for re-use Low impact on freezer Large thermal mass impacts local freezer •1/3 the heat impact on freezer compared area to alcohol-filled units •Large heat capacity removed from alcohol impacts nearby samples 21
- 22. Customer quotes StanfordUniversity “We run a registry, in which large amounts of PBMCs are processed for long-term cyropreservation. After testing the CoolCell out, we found slightly better cell viability (>90%) than our current cell freezing containers, and there is no Isopropanol waste generated. Overall, the CoolCell has proven to us to set a new bar in cryopreservation.” Merck Pharmaceuticals “I have already purchased 21 CoolCells, I have obtained a CoolBox, and I recently ordered a 96well flat bottom CoolBox system. All these will be used for our assays. I need one more product to complete my collection of ice-free cooling devices. I already passed on my evaluation of the CoolCell and the other groups are pretty excited and are going to use them.“ UC Berkeley “Our lab at UC Berkeley recently tried out demo models for the CoolRackM90, CoolSink, and Thermal Tray and we loved them and thought they were very useful. We were just amazed by how quickly the Cool Rack chills our tubes within minutes while preventing the possibility of water getting in and contaminating our samples. ……We decided to get all of them after trying them out due to their effectiveness. Our lab manager has been looking for a company that was offering such products and fortunately, we've the right place!” 22
- 23. Genentech Amgen Pfizer Roche Harvard MIT CalTech USCF Glaxo Bristol Myers Squibb Novartis Translational Genomics Gilead Sciences Merck MedImmune Genpharm Elan Pharmaceuticals Centocor Boehringer Ingelheim Oncomed NIH/NCI Takeda Pharmaceuticals CDC Covance XOMA Quest Diagnostics Otsuka America Pharmaceuticals Thank you. Salk Institute Burnham Institute Scripps Institute Stanford University Yale Genzyme EMD Serono Shire HGT Alnylam Pharmaceuticals Cell-System Jackson Labs Beth Israel Deaconess Hospital HHMI Whitehead Institute Harvard Systems Biology Michelle Nemits Exelixis Charles River Labs Sanofi Pasteur Penn State VP Sales & Business Development Lawrence Berkeley Lab Batelle UC Berkeley Mobile: 415-336-4415 Cedars Sinai Hospital Cleveland Clinic IDEXX Biogen Perkin Elmer Genetics Blood Systems Research Inst. BC Cancer Research Center USC Pacific Bioscience UCLA Precision Therapeutics Northwestern 23