The accumulation of partial glycerides such as monoglyceride (MG) and diglyceride (DG) is one of the rate-limiting steps in plant oil methanolysis catalyzed by Rhizopus oryzae producing triacylglycerol lipase.
To convert partial glycerides efficiently into their corresponding methyl esters (MEs), we attempted to use a mono- and diacylglycerol lipase (mdlB) derived from Aspergillus oryzae. By considering cost efficiency,
R. oryzae and recombinant mdlB-producing A. oryzae were immobilized independently within polyurethane foam biomass support particles and directly utilized as a whole-cell biocatalyst. The mdlBproducing A. oryzae effectively exhibited substrate specificity toward MG and DG and was then used
for the methanolysis of intermediate products (approximately 82% ME), which were produced using R.
oryzae. In the presence of 5% water, the use of mdlB-producing A. oryzae resulted in less than 0.1% of MG and DG, whereas a considerable amount of triglyceride was present in the final reaction mixture. On
the basis of these results, we developed a packed-bed reactor (PBR) system, which consists of the first column with R. oryzae and the second column containing both R. oryzae and mdlB-producing A. oryzae.
Ten repeated-batch methanolysis cycles in the PBR maintained a high ME content of over 90% with MG
and DG at 0.08–0.69 and 0.22–1.45%, respectively, indicating that the PBR system can be used for longterm repeated batchmethanolysis with partial glycerides at lowlevels. The proposedmethod is therefore effective for improving enzymatic biodiesel production.
Use of mono- and diacylglycerol lipase as immobilized fungal whole cells to c...UMTC
Use of mono- and diacylglycerol lipase as immobilized fungal whole cells to
convert residual partial glycerides enzymatically into fatty acid methyl esters
CHARACTERIZATION OF MUSTY ODOR IN STREPTOMYCES SP. S10 UNDER NUTRIENT STRE...SHAHIRAH
The purpose of this study is to investigate the effect of phosphate concentration to the taste and odor production from tropical country, particularly in Malaysia. Musty odor is usually related to the presence of geosmin and 2-methylisoborneol (2-MIB) and the odor can be noticed even at concentrations as low as 10 ng/L. Actinomycetes are known to be the source of geosmin and 2-MIB. The existence of these compounds is still not actively researched in Malaysia since the problem is not severe yet. However, if these odors continue to exist in the drinking water system, it will impact the water system management with economic losses and complaints from customers. Streptomyces spp. strain S10, which was isolated from a water reservoir in Malaysia, has the ability to produce geosmin when cultivated in basal salt (BS) solid medium, but no 2-MIB production occurred during growth in BS medium. Strain S10 could produce higher levels of geosmin when the phosphate concentration was limited to 0.05 mg/L, with a yield of 17.53 ± 3.12 × 105 ng/L, compared with growth in BS medium and this yield was significantly different (P < 0.05) from the yield produced in the presence of phosphate at the other concentrations tested. This study found that S10 produced more geosmin when phosphate was limited. It can be concluded that the amount of phosphate in the water surface should be monitored regularly to prevent the production of these odor-producing strains in tropical areas.
The accumulation of partial glycerides such as monoglyceride (MG) and diglyceride (DG) is one of the rate-limiting steps in plant oil methanolysis catalyzed by Rhizopus oryzae producing triacylglycerol lipase.
To convert partial glycerides efficiently into their corresponding methyl esters (MEs), we attempted to use a mono- and diacylglycerol lipase (mdlB) derived from Aspergillus oryzae. By considering cost efficiency,
R. oryzae and recombinant mdlB-producing A. oryzae were immobilized independently within polyurethane foam biomass support particles and directly utilized as a whole-cell biocatalyst. The mdlBproducing A. oryzae effectively exhibited substrate specificity toward MG and DG and was then used
for the methanolysis of intermediate products (approximately 82% ME), which were produced using R.
oryzae. In the presence of 5% water, the use of mdlB-producing A. oryzae resulted in less than 0.1% of MG and DG, whereas a considerable amount of triglyceride was present in the final reaction mixture. On
the basis of these results, we developed a packed-bed reactor (PBR) system, which consists of the first column with R. oryzae and the second column containing both R. oryzae and mdlB-producing A. oryzae.
Ten repeated-batch methanolysis cycles in the PBR maintained a high ME content of over 90% with MG
and DG at 0.08–0.69 and 0.22–1.45%, respectively, indicating that the PBR system can be used for longterm repeated batchmethanolysis with partial glycerides at lowlevels. The proposedmethod is therefore effective for improving enzymatic biodiesel production.
Use of mono- and diacylglycerol lipase as immobilized fungal whole cells to c...UMTC
Use of mono- and diacylglycerol lipase as immobilized fungal whole cells to
convert residual partial glycerides enzymatically into fatty acid methyl esters
CHARACTERIZATION OF MUSTY ODOR IN STREPTOMYCES SP. S10 UNDER NUTRIENT STRE...SHAHIRAH
The purpose of this study is to investigate the effect of phosphate concentration to the taste and odor production from tropical country, particularly in Malaysia. Musty odor is usually related to the presence of geosmin and 2-methylisoborneol (2-MIB) and the odor can be noticed even at concentrations as low as 10 ng/L. Actinomycetes are known to be the source of geosmin and 2-MIB. The existence of these compounds is still not actively researched in Malaysia since the problem is not severe yet. However, if these odors continue to exist in the drinking water system, it will impact the water system management with economic losses and complaints from customers. Streptomyces spp. strain S10, which was isolated from a water reservoir in Malaysia, has the ability to produce geosmin when cultivated in basal salt (BS) solid medium, but no 2-MIB production occurred during growth in BS medium. Strain S10 could produce higher levels of geosmin when the phosphate concentration was limited to 0.05 mg/L, with a yield of 17.53 ± 3.12 × 105 ng/L, compared with growth in BS medium and this yield was significantly different (P < 0.05) from the yield produced in the presence of phosphate at the other concentrations tested. This study found that S10 produced more geosmin when phosphate was limited. It can be concluded that the amount of phosphate in the water surface should be monitored regularly to prevent the production of these odor-producing strains in tropical areas.
Methyl Red (MR) and Voges-Proskauer (VP) Test principle, Method, Interpretation & QC #MR & VP
Mallu Medicos Lounge
As the channel name suggests, our channel will be a perfect lounge for the malayali medicos..we wil be covering videos which will be like lecture classes related to the subjects biochemistry and microbiology in which we are specialised.. It will be a better learning experience for the students especially for those who are not able to understand and follow the normal classes in college..we assure the students that you will get a basic idea regarding the topic and extra reading can be done from the reference textbooks..
Our Partner Channel
Health & Voyage channel link - https://youtu.be/nzKqRVjlwc0
Qualification
Maneesha M Joseph
MSc MLT (Microbiology)
Assistant Professor
Baby memorial college of allied Health science
Kozhikode
#Methyl Red Test
#Voges-Proskauer Test
#MRVP Procedure
#MRVP Tests
#MR-VP Tests
#VP (Voges Proskaeur) Test
#MR/VP Test
#Methyl red (MR) and Voges-Proskauer (VP)
#Methyl red test in Microbiology
#Medical
#Microbiology
#MR/VP Test malayalam lecturer
#Mallu Medicos Lounge
#MalluMedicosLounge
#MLT
#Channel introduction
#HealthAndVoyage
Tinospora Cordifolia the magical Herb (Giloy)Vedant Patel
Advanced Herbal drug technology,A Presentation on
Extraction, isolation and standardization of Phytochemicals in Crude extract of Tinospora Cordifolia (Giloy, gulvel,giloe, Amrita,garo).It Shows presence of flavonoids and Alkaloids which shows Anti-cancer,Anti-oxidants, Anti-viral, Anti-inflammatory and Anti-allergic activity by boosting host immune system. it also involves different test for identification of Alkaloids, flavonoids, saponins,tanins, glycoside.
A discussion on the media and biochemical tests as discussed by Ms. Caryl Villalon, RN, MT. Covers the descriptions of the media and biochemical tests. How to perform the tests, properties of the tests, media and reagents used, and the results of the test. Pictures of positive and negative results are also shown in the slide.
Sugar fermentation tests, Cetrimide agar medium, Hugh Leifson medium Shivam kumar Sriwas
Combined presentation on:-
1. Sugar fermentation tests
2. Cetrimide agar
3. Hugh Leifson medium
Covering principle, preparation of the medium, test protocol, result interpretations, test conclusions.
In experimemt of isolation of vactwria using special purpose media W.pdfmichaelazach6427
In experimemt of isolation of vactwria using special purpose media What is the chemical
composition of EMB agar? The mannitol salt agar?
Solution
The special purpose media used for isolation of bacteria or also called enrichment media where
you selectively allow one of the organims to grow with selective media composition. So the
EMB agar also called as Eosin Methylene blue agar media chemical composition is peptone,
lactose, dipotassium phosphate, eosin Y (dye), methylene blue (dye), and agar.
Mannitol salt agar is also a differential growth media, It contains a high concentration (about
7.5%-10%) of salt (NaCl), making it selective for Gram-positive bacteria since this level of salt
is inhibitory to most other bacteria. It is also a differential medium for mannitol-fermenting
staphylococci, containing carbohydrate mannitol and the indicator phenol red, a pH indicator for
detecting acid produced by mannitol-fermenting staphylococci..
Methyl Red (MR) and Voges-Proskauer (VP) Test principle, Method, Interpretation & QC #MR & VP
Mallu Medicos Lounge
As the channel name suggests, our channel will be a perfect lounge for the malayali medicos..we wil be covering videos which will be like lecture classes related to the subjects biochemistry and microbiology in which we are specialised.. It will be a better learning experience for the students especially for those who are not able to understand and follow the normal classes in college..we assure the students that you will get a basic idea regarding the topic and extra reading can be done from the reference textbooks..
Our Partner Channel
Health & Voyage channel link - https://youtu.be/nzKqRVjlwc0
Qualification
Maneesha M Joseph
MSc MLT (Microbiology)
Assistant Professor
Baby memorial college of allied Health science
Kozhikode
#Methyl Red Test
#Voges-Proskauer Test
#MRVP Procedure
#MRVP Tests
#MR-VP Tests
#VP (Voges Proskaeur) Test
#MR/VP Test
#Methyl red (MR) and Voges-Proskauer (VP)
#Methyl red test in Microbiology
#Medical
#Microbiology
#MR/VP Test malayalam lecturer
#Mallu Medicos Lounge
#MalluMedicosLounge
#MLT
#Channel introduction
#HealthAndVoyage
Tinospora Cordifolia the magical Herb (Giloy)Vedant Patel
Advanced Herbal drug technology,A Presentation on
Extraction, isolation and standardization of Phytochemicals in Crude extract of Tinospora Cordifolia (Giloy, gulvel,giloe, Amrita,garo).It Shows presence of flavonoids and Alkaloids which shows Anti-cancer,Anti-oxidants, Anti-viral, Anti-inflammatory and Anti-allergic activity by boosting host immune system. it also involves different test for identification of Alkaloids, flavonoids, saponins,tanins, glycoside.
A discussion on the media and biochemical tests as discussed by Ms. Caryl Villalon, RN, MT. Covers the descriptions of the media and biochemical tests. How to perform the tests, properties of the tests, media and reagents used, and the results of the test. Pictures of positive and negative results are also shown in the slide.
Sugar fermentation tests, Cetrimide agar medium, Hugh Leifson medium Shivam kumar Sriwas
Combined presentation on:-
1. Sugar fermentation tests
2. Cetrimide agar
3. Hugh Leifson medium
Covering principle, preparation of the medium, test protocol, result interpretations, test conclusions.
In experimemt of isolation of vactwria using special purpose media W.pdfmichaelazach6427
In experimemt of isolation of vactwria using special purpose media What is the chemical
composition of EMB agar? The mannitol salt agar?
Solution
The special purpose media used for isolation of bacteria or also called enrichment media where
you selectively allow one of the organims to grow with selective media composition. So the
EMB agar also called as Eosin Methylene blue agar media chemical composition is peptone,
lactose, dipotassium phosphate, eosin Y (dye), methylene blue (dye), and agar.
Mannitol salt agar is also a differential growth media, It contains a high concentration (about
7.5%-10%) of salt (NaCl), making it selective for Gram-positive bacteria since this level of salt
is inhibitory to most other bacteria. It is also a differential medium for mannitol-fermenting
staphylococci, containing carbohydrate mannitol and the indicator phenol red, a pH indicator for
detecting acid produced by mannitol-fermenting staphylococci..
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
Comparative structure of adrenal gland in vertebrates
Biochemicaltests (1)
1. Test Medium Substrate Product(s) Reagent pH indicator + appearance organisms +
Amylase starch agar starch glucose, maltose.
dextrins
iodine colorless zone
after iodine
B. subtilis
Caseinase skim milk agar casein amino acids,
peptides
clear zone B. subtilis
DNase DNase agar DNA nucleotides methyl green colorless zone S. aureus
Lipase spirit blue agar lipid glycerol. fatty
acids
blue/clear zone,
blue ppt.
S. aureus
Eosin Methylene
Blue (EMB) agar 1
EMB agar lactose organic acids metallic green;
fish eyes
E. coli;
E. aerogenes
MacConkey’s agar 1
MacConkey’s
agar
lactose organic acids neutral red pink (+ ppt.) E. coli;
E. aerogenes
Mannitol Salt Agar
(MSA) 2
MSA mannitol organic acids phenol red yellow media S. aureus
Indole TSB tryptophan indole Kovac’s reagent red at surface E. coli
Methyl Red MRVP broth glucose organic acids methyl red red throughout E. coli
Voges-Proskauer MRVP broth glucose non-acids VP-A & VP-B red at surface E. aerogenes
Citrate Utilization Simmons’ citrate
agar
citrate Na2CO3 bromthymol
blue
royal blue E. aerogenes
TSI: H2S Gas TSI agar iron (sulfate) iron sulfide phenol red black ppt. S. typhimurium
TSI: Sugar Ferm. TSI agar glucose, lactose,
sucrose
organic acids phenol red yellow P. vulgaris
(also + for H2S)
Urease (Christensen’s)
urea agar
urea ammonia, CO2 phenol red pink Proteus
Catalase hydrogen
peroxide (H2O2)
water, oxygen bubbles Staphylococci
Coagulase fibrinogen clumping factor-
fibrinogen complex
blue ppt., solid S. aureus
Hemolysis: α 5% sheep’s blood
agar
hemoglobin (red) biliverdin
(green/brown)
green/brown
zone
S. pneumoniae
Hemolysis: β 5% sheep’s blood
agar
red blood cells lysed red blood
cells
clear zone S. pyogenes,
S. aureus
Carb. Ferm. Series glucose, lactose,
sucrose broth
glucose, lactose,
sucrose
organic acids,
CO2
phenol red yellow, CO2 in
Durham tube
Nitrate Reduction tryptic nitrate
broth
nitrate nitrite; ammonia nitrate A & B,
zinc
red color
Oxidase blue color P. aeruginosa
Litmus litmus milk casein, lactose various litmus various
Microbiology: Biochemical Tests
1
selective for gram-negative bacteria; differential results indicated 2
selective for salt-tolerant staphylococci; differential results indicated
