You want a flexible and multi-usable GC or GCMS system with increased application range and detection limits? From 1ul injection till automated pyrolysis GCMS.
1) Headspace gas chromatography allows the analysis of volatile compounds in samples without directly injecting the sample into the GC. This prevents non-volatile residues from interfering.
2) Key factors that affect headspace sensitivity and analysis include temperature, phase ratio (sample volume), partition coefficient, and concentration - which influence the concentration of analyte in the headspace.
3) Techniques like multiple headspace extraction and cryofocusing can be used to concentrate analytes and improve detection of compounds in complex samples.
Gas chromotography (Histroy, Importance, Classification)Talal Khan
This Presentation gives the brief introduction of Gas Chromatography, its History, and its Classifications.
It also describes the mechanism through which it is operated.
A separation technique in which the mobile phase is a gas. Gas chromatography is always carried out in a column.
Separating mixtures of gases or volatile materials based primarily on their physical properties.
This document provides an overview of gas chromatography. It begins by defining chromatography and tracing the history of gas chromatography from its origins in 1903 to its development in the 1940s-1950s. It then describes the basic components and working principles of gas chromatography, including the mobile phase, stationary phase, factors that influence separation, and common instrumentation. It also discusses different types of chromatography techniques and gas chromatography columns. In summary, the document provides a comprehensive introduction to gas chromatography, its history, principles, instrumentation and applications.
These slides give an introduction to gas chromatography, It also guides analyst to a proper selection of liner, column, and some main operating conditions.
This document discusses HPLC columns, including:
1. Silica is commonly used as the surface for HPLC columns, with silanols bonding to the surface. Pore size and surface area impact analyte retention and loading capacity.
2. Column particle sizes have decreased over time from 100 μm to below 2 μm, increasing theoretical plate counts. Column dimensions and particle sizes are selected based on the application.
3. Pore size should be larger than analyte molecules to allow entry without hindrance. Pore sizes of 60-80Å or 95-300Å are recommended for small molecules or proteins, respectively.
Gas chromatography is a technique used to separate components of a mixture. It was invented in 1901 by Russian botanist Mikhail Tswett. Key developments include John Porter Martin developing the first gas-liquid chromatograph in the 1950s. Gas chromatography-mass spectrometry allows identification of separated components. The technique works by vaporizing a sample and carrying it by a carrier gas through a column coated with a stationary phase, separating components based on how they partition between the mobile and stationary phases.
1) Headspace gas chromatography allows the analysis of volatile compounds in samples without directly injecting the sample into the GC. This prevents non-volatile residues from interfering.
2) Key factors that affect headspace sensitivity and analysis include temperature, phase ratio (sample volume), partition coefficient, and concentration - which influence the concentration of analyte in the headspace.
3) Techniques like multiple headspace extraction and cryofocusing can be used to concentrate analytes and improve detection of compounds in complex samples.
Gas chromotography (Histroy, Importance, Classification)Talal Khan
This Presentation gives the brief introduction of Gas Chromatography, its History, and its Classifications.
It also describes the mechanism through which it is operated.
A separation technique in which the mobile phase is a gas. Gas chromatography is always carried out in a column.
Separating mixtures of gases or volatile materials based primarily on their physical properties.
This document provides an overview of gas chromatography. It begins by defining chromatography and tracing the history of gas chromatography from its origins in 1903 to its development in the 1940s-1950s. It then describes the basic components and working principles of gas chromatography, including the mobile phase, stationary phase, factors that influence separation, and common instrumentation. It also discusses different types of chromatography techniques and gas chromatography columns. In summary, the document provides a comprehensive introduction to gas chromatography, its history, principles, instrumentation and applications.
These slides give an introduction to gas chromatography, It also guides analyst to a proper selection of liner, column, and some main operating conditions.
This document discusses HPLC columns, including:
1. Silica is commonly used as the surface for HPLC columns, with silanols bonding to the surface. Pore size and surface area impact analyte retention and loading capacity.
2. Column particle sizes have decreased over time from 100 μm to below 2 μm, increasing theoretical plate counts. Column dimensions and particle sizes are selected based on the application.
3. Pore size should be larger than analyte molecules to allow entry without hindrance. Pore sizes of 60-80Å or 95-300Å are recommended for small molecules or proteins, respectively.
Gas chromatography is a technique used to separate components of a mixture. It was invented in 1901 by Russian botanist Mikhail Tswett. Key developments include John Porter Martin developing the first gas-liquid chromatograph in the 1950s. Gas chromatography-mass spectrometry allows identification of separated components. The technique works by vaporizing a sample and carrying it by a carrier gas through a column coated with a stationary phase, separating components based on how they partition between the mobile and stationary phases.
Inductively coupled plasma mass spectrometryMohamed Fayed
ICP-MS has been widely used for elemental analysis in various fields such as environmental, clinical, and geological applications. It functions by inductively coupling plasma to generate ions from a sample, which are then sorted by mass and detected. Key advantages include excellent detection limits in the parts per trillion range, ability to detect multiple elements simultaneously, and capacity for isotopic analysis. The instrument features a sample introduction system that turns the sample into an aerosol, an ionization region where the plasma converts atoms into ions, ion extraction interfaces that transport ions into the mass spectrometer, and ion optics that focus the ion beam.
This document provides possible causes and suggested remedies for common gas chromatography (GC) troubleshooting issues. Some key issues addressed include no peaks, missing peaks, too small peaks, increasing or decreasing retention times, declining or rising baselines, interfering peaks, spikes, ghost peaks, broad peaks, fronting, tailing, cut tops, negative peaks, and double peaks. Suggested remedies include checking detector/electronics, gas flow, column installation, temperature programming, sample concentration, and column condition. The document is authored by Hassan Alnajem and provides troubleshooting guidance for resolving a variety of common GC performance problems.
Chromatography is a physical separation method that separates a mixture into its individual components. It works by distributing the analytes between a stationary and mobile phase. There are different types of chromatography defined by the mobile and stationary phases used, including gas chromatography (GC) which uses a gas as the mobile phase. GC is commonly used with capillary columns for high resolution separation of volatile organic compounds. It works by separating compounds based on their partitioning between the stationary phase coating on the column and the carrier gas mobile phase.
This document discusses key terminology and parameters used in headspace gas chromatography (HSGC). It explains the differences between a loop-pressure system and a pressure balanced system. Key terms defined include oven temperature, needle temperature, transfer line temperature, thermostatting time, pressurization time, injection time, withdrawal time, GC cycle time, and vial venting time. The document also covers loop fill time, loop equilibration time, inject time, and multiple headspace extraction.
This document provides an overview of gas chromatography. It describes the basic components and process of gas chromatography including the carrier gas, sample injection system, columns, temperature and pressure programming, and common detectors like the thermal conductivity detector and flame ionization detector. The goal of gas chromatography is to separate a mixture into individual components using a mobile gas phase and stationary column packing material over time based on differences in how components partition between the two phases.
This ppt consist of basic principle of GC-MS, instrumentation of GC-MS, components of GC-MS ,Advantages and disadvantages of GC-MS and application of GC-MS
Gas chromatography is a process that separates components of a mixture using an inert gas as the mobile phase. It involves vaporizing a sample and injecting it onto the head of a chromatographic column containing a stationary phase. The sample is then transported through the column by the flow of the mobile phase gas. Common detectors used in gas chromatography include the thermal conductivity detector, flame ionization detector, and electron capture detector. The flame ionization detector is highly sensitive for detecting organic compounds containing carbon. The electron capture detector is selective for compounds containing electronegative atoms like halogens.
This document provides an overview of high performance liquid chromatography (HPLC) presented by Ravi Pratap Pulla. It introduces HPLC and its history. Key topics covered include HPLC components like columns and systems. Applications to pharmaceutical analysis are discussed. The document also reviews some basic HPLC terminology and concepts.
This document describes a study comparing two methods for determining distillation yield curves in heavy crude oils: ASTM D-5307 simulated distillation using gas chromatography (SIMDIS GC) and ASTM D-2892 physical distillation. The researchers found large uncertainties using ASTM D-5307 and made some improvements. They analyzed Mexican Istmo and Maya crude oil samples using both methods, finding good agreement between the results. The statistical analysis supported SIMDIS GC as a good alternative for measuring distillation curves in heavy crude oils.
This document discusses Thermogravimetric Analysis (TGA) and Differential Scanning Calorimetry (DSC). TGA measures the change in weight of a sample during heating or cooling, while DSC measures the heat absorbed or released by a sample during phase transitions or chemical reactions. Both techniques provide information about physical and chemical changes in materials as functions of temperature. The document describes the principles, instrumentation, experimental procedures, sources of error, and applications of TGA and DSC for characterizing materials.
Dissolution procedure development and validation, USP 1092Md. Saddam Nawaz
This document discusses the development and validation of dissolution procedures according to USP<1092>. It provides general comments on the purpose of dissolution testing and discusses key aspects of developing a discriminating and reproducible method, including choice of medium, apparatus, study design, sampling, and validation. The document outlines factors to consider for various dosage forms and provides examples of typical dissolution conditions and acceptance criteria.
This document discusses gas chromatography (GC). It notes that there are two main types: GSC and GLC. GLC is more widely used due to more available stationary phases. GLC uses partition as its principle, with a liquid stationary phase coated on a solid support and a gas mobile phase. Key criteria for GC are volatility and thermostability of compounds. Common carrier gases are nitrogen, helium, and hydrogen. Detectors discussed include thermal conductivity, flame ionization, electron capture, and nitrogen phosphorous detectors. Recorders and integrators are used to record and provide additional data on chromatographic peaks.
Reviews the SFC technique along with applications and case studies.
Use of animations disturbs the view. For full ppt, drop me a mail at varadbende96@gmail.com
Gas chromatography is a technique used to separate volatile organic compounds using a mobile gas phase and stationary phase in a column. Key components include an injection port to introduce the sample, an oven to heat the column and volatilize compounds, and a detector. Differences in how compounds partition between the mobile and stationary phases allows separation as they migrate through the column at different rates. Common detectors include the flame ionization detector.
this presentation presents introduction about high performance thin layer chromatography, its features, principle and instrumentation along with its applications. it also gives comparison between TLC and HPTLC. instrumentation is given in a sequence for easier understanding of instrument.
Esco Containment Barrier Isolator (CBI) facilitates the isolation of a product or process while providing the required conditions for a sterile/ aseptic environment. It is configured to operate at positive or negative pressure. This equipment provides a comprehensive range of personnel
and product protection in addition to protection for the surrounding work areas and the environment.
CBI's design has complete compliance to PIC/s and EU cGMP standardswith its 19 mm radius coved internal corners in a single piece chamber (no perforations or grilles for contaminants to be trapped on all 4 corners). Its Rear Return Filter ensures that ducts are not contaminated. The system comes in either recirculatory or single pass airflow.
Applications
• Pharmacy Compounding (Chemotherapy/TPN)
• As a Class III Cabinet for Biosafety Levels (BSL) 3 and 4
• Small Batch Sterility Testing
• Small-scale Potent Material Handling
• Cell Processing
• Aseptic Processing
• Research and Development
aixergee - Process Optimization for the Cement IndustryLOESCHE
The document discusses process optimization for the cement industry. It describes how process optimization can help cement producers get better results without major investment by identifying limitations, understanding root causes, and providing solutions. It provides an overview of aixergee's approach to process optimization, which includes data collection, analysis using modeling and simulations, developing proposals for improvements, and engineering support. It also includes several examples of process optimizations performed for cement plants.
Inductively coupled plasma mass spectrometryMohamed Fayed
ICP-MS has been widely used for elemental analysis in various fields such as environmental, clinical, and geological applications. It functions by inductively coupling plasma to generate ions from a sample, which are then sorted by mass and detected. Key advantages include excellent detection limits in the parts per trillion range, ability to detect multiple elements simultaneously, and capacity for isotopic analysis. The instrument features a sample introduction system that turns the sample into an aerosol, an ionization region where the plasma converts atoms into ions, ion extraction interfaces that transport ions into the mass spectrometer, and ion optics that focus the ion beam.
This document provides possible causes and suggested remedies for common gas chromatography (GC) troubleshooting issues. Some key issues addressed include no peaks, missing peaks, too small peaks, increasing or decreasing retention times, declining or rising baselines, interfering peaks, spikes, ghost peaks, broad peaks, fronting, tailing, cut tops, negative peaks, and double peaks. Suggested remedies include checking detector/electronics, gas flow, column installation, temperature programming, sample concentration, and column condition. The document is authored by Hassan Alnajem and provides troubleshooting guidance for resolving a variety of common GC performance problems.
Chromatography is a physical separation method that separates a mixture into its individual components. It works by distributing the analytes between a stationary and mobile phase. There are different types of chromatography defined by the mobile and stationary phases used, including gas chromatography (GC) which uses a gas as the mobile phase. GC is commonly used with capillary columns for high resolution separation of volatile organic compounds. It works by separating compounds based on their partitioning between the stationary phase coating on the column and the carrier gas mobile phase.
This document discusses key terminology and parameters used in headspace gas chromatography (HSGC). It explains the differences between a loop-pressure system and a pressure balanced system. Key terms defined include oven temperature, needle temperature, transfer line temperature, thermostatting time, pressurization time, injection time, withdrawal time, GC cycle time, and vial venting time. The document also covers loop fill time, loop equilibration time, inject time, and multiple headspace extraction.
This document provides an overview of gas chromatography. It describes the basic components and process of gas chromatography including the carrier gas, sample injection system, columns, temperature and pressure programming, and common detectors like the thermal conductivity detector and flame ionization detector. The goal of gas chromatography is to separate a mixture into individual components using a mobile gas phase and stationary column packing material over time based on differences in how components partition between the two phases.
This ppt consist of basic principle of GC-MS, instrumentation of GC-MS, components of GC-MS ,Advantages and disadvantages of GC-MS and application of GC-MS
Gas chromatography is a process that separates components of a mixture using an inert gas as the mobile phase. It involves vaporizing a sample and injecting it onto the head of a chromatographic column containing a stationary phase. The sample is then transported through the column by the flow of the mobile phase gas. Common detectors used in gas chromatography include the thermal conductivity detector, flame ionization detector, and electron capture detector. The flame ionization detector is highly sensitive for detecting organic compounds containing carbon. The electron capture detector is selective for compounds containing electronegative atoms like halogens.
This document provides an overview of high performance liquid chromatography (HPLC) presented by Ravi Pratap Pulla. It introduces HPLC and its history. Key topics covered include HPLC components like columns and systems. Applications to pharmaceutical analysis are discussed. The document also reviews some basic HPLC terminology and concepts.
This document describes a study comparing two methods for determining distillation yield curves in heavy crude oils: ASTM D-5307 simulated distillation using gas chromatography (SIMDIS GC) and ASTM D-2892 physical distillation. The researchers found large uncertainties using ASTM D-5307 and made some improvements. They analyzed Mexican Istmo and Maya crude oil samples using both methods, finding good agreement between the results. The statistical analysis supported SIMDIS GC as a good alternative for measuring distillation curves in heavy crude oils.
This document discusses Thermogravimetric Analysis (TGA) and Differential Scanning Calorimetry (DSC). TGA measures the change in weight of a sample during heating or cooling, while DSC measures the heat absorbed or released by a sample during phase transitions or chemical reactions. Both techniques provide information about physical and chemical changes in materials as functions of temperature. The document describes the principles, instrumentation, experimental procedures, sources of error, and applications of TGA and DSC for characterizing materials.
Dissolution procedure development and validation, USP 1092Md. Saddam Nawaz
This document discusses the development and validation of dissolution procedures according to USP<1092>. It provides general comments on the purpose of dissolution testing and discusses key aspects of developing a discriminating and reproducible method, including choice of medium, apparatus, study design, sampling, and validation. The document outlines factors to consider for various dosage forms and provides examples of typical dissolution conditions and acceptance criteria.
This document discusses gas chromatography (GC). It notes that there are two main types: GSC and GLC. GLC is more widely used due to more available stationary phases. GLC uses partition as its principle, with a liquid stationary phase coated on a solid support and a gas mobile phase. Key criteria for GC are volatility and thermostability of compounds. Common carrier gases are nitrogen, helium, and hydrogen. Detectors discussed include thermal conductivity, flame ionization, electron capture, and nitrogen phosphorous detectors. Recorders and integrators are used to record and provide additional data on chromatographic peaks.
Reviews the SFC technique along with applications and case studies.
Use of animations disturbs the view. For full ppt, drop me a mail at varadbende96@gmail.com
Gas chromatography is a technique used to separate volatile organic compounds using a mobile gas phase and stationary phase in a column. Key components include an injection port to introduce the sample, an oven to heat the column and volatilize compounds, and a detector. Differences in how compounds partition between the mobile and stationary phases allows separation as they migrate through the column at different rates. Common detectors include the flame ionization detector.
this presentation presents introduction about high performance thin layer chromatography, its features, principle and instrumentation along with its applications. it also gives comparison between TLC and HPTLC. instrumentation is given in a sequence for easier understanding of instrument.
Esco Containment Barrier Isolator (CBI) facilitates the isolation of a product or process while providing the required conditions for a sterile/ aseptic environment. It is configured to operate at positive or negative pressure. This equipment provides a comprehensive range of personnel
and product protection in addition to protection for the surrounding work areas and the environment.
CBI's design has complete compliance to PIC/s and EU cGMP standardswith its 19 mm radius coved internal corners in a single piece chamber (no perforations or grilles for contaminants to be trapped on all 4 corners). Its Rear Return Filter ensures that ducts are not contaminated. The system comes in either recirculatory or single pass airflow.
Applications
• Pharmacy Compounding (Chemotherapy/TPN)
• As a Class III Cabinet for Biosafety Levels (BSL) 3 and 4
• Small Batch Sterility Testing
• Small-scale Potent Material Handling
• Cell Processing
• Aseptic Processing
• Research and Development
aixergee - Process Optimization for the Cement IndustryLOESCHE
The document discusses process optimization for the cement industry. It describes how process optimization can help cement producers get better results without major investment by identifying limitations, understanding root causes, and providing solutions. It provides an overview of aixergee's approach to process optimization, which includes data collection, analysis using modeling and simulations, developing proposals for improvements, and engineering support. It also includes several examples of process optimizations performed for cement plants.
This document provides information about the presenter and the topic they will be presenting on, which is gas chromatography. It outlines the learning objectives, contents, and activities for the presentation. The presentation will cover the history, principle, theory, instrumentation, advantages, and disadvantages of gas chromatography. It will discuss the essential parts of a gas chromatograph such as the sample injection system, columns, detectors, and carrier gas. The activities include drawing the basic setup of a GC, identifying the most commonly used carrier gas, predicting compound elution order, and listing factors that affect resolution.
Gas Chromatography Thermo Fisher ScientificYuniarHasani
What is Chromatography?
Separation process that is achieved by distributing the analytes to be separated
between a mobile phase and a stationary phase.
Sample transported by mobile phase
Some components in sample interact more strongly with stationary phase
and are more strongly retained
The SenseTek automatic purge unit is designed to automatically clean the pipes of Stratos aspirating fire detection systems on a programmed schedule. It uses compressed air to blow through the pipes to remove pollutants. It has a PLC controller with 7 cleaning programs of varying intervals and can be powered by the detector's power supply. It is suitable for heavy polluted environments like warehouses, production facilities, and stables to prevent the pipes from clogging and extend the life of the detector's filter.
www.envimart.vn - ĐT: 028 77727979 - sales@envimart.vn - Nền tảng cung cấp thiết bị, vật tư ngành nước và môi trường. Chuyên cung cấp vật tư cho dự án xử lý nước sạch, nước thải và môi trường. Envimart luôn đồng hành, tin cậy với đối tác nhà thầu, nhà tích hợp và người sử dụng.
This document provides information on various high pressure and mixing equipment produced by ILSHIN AUTOCLAVE, including:
1. High pressure reactors for reactions up to 8000 psi and 250°C.
2. Pressure ovens for curing and sterilization up to 1000 bar and 120°C.
3. Isostatic presses applying isostatic force in liquids like oil or water up to 6000 bar.
4. Supercritical carbon dioxide systems for extraction and drying using supercritical CO2.
5. Agitators, high shear mixers, and three roll mills for efficient mixing of materials up to 70,000 centipoise viscosity.
The document discusses using a multi-functional injection port to expand the utility of gas chromatography. It describes six analytical techniques - direct split injection, evolved gas analysis, thermal desorption, simple pyrolysis, thermal desorption followed by pyrolysis, and heart-cut GC/MS analysis of volatile fractions - that can be performed using the injection port. It also discusses how the programmable injector can perform a pre-separation, and two-dimensional gas chromatography. The injection port allows for thermal extraction of samples via thermal desorption, evolved gas analysis, or pyrolysis, providing advantages of greater accuracy, higher precision, and time savings over conventional sample preparation methods.
This document summarizes the features of a CO2 incubator model. It has a PID microprocessor controller, heated outer door, tempered glass inner door, and maintains temperature uniformity while minimizing condensation. It has alarms for temperature and CO2 levels out of tolerance. It uses infrared sensors for fast CO2 recovery and stability. Extensive copper is used throughout to control contamination, and it maintains temperature, CO2 levels, and humidity to replicate mammalian environments for tissue and cell culture. It comes with a 5-year parts warranty and lifetime warranty on the water jacket chamber.
The document summarizes an analysis of residual solvents using an Agilent 8890 GC system according to a USP method. Key aspects include:
1) The Agilent 8890 GC was configured with dual FIDs, two columns, and an autosampler to analyze class 1-3 residual solvents.
2) Samples were prepared according to the USP method and run in triplicate to determine repeatability of retention times and peak areas.
3) Results for various class 1-3 solvents showed good repeatability with retention time RSDs below 1% and area RSDs below 5% on both columns.
PCR
PCR is a method widely used in Molecular biology to make many copies of a specific DNA segment.
Using PCR it is possible to generate thousand millions of copies of a particular section of DNA from very small amount of DNA.
PCR was originally developed in 1983 by the American Biochemists Kary Mullis. He was awarded the Nobel Prize in Chemistry in 1993 for his pioneering work.
The SCO6AD CO2 incubator features a dry heat decontamination cycle that maintains 180°C for 120 minutes, satisfying global standards. It has the shortest decontamination cycle time on the market without needing to remove the IR CO2 sensor. Extensive copper and a decontamination token add reassurance that microbes will not affect test results. The incubator has precise temperature control, IR CO2 sensors for fast recovery, and a 5-year warranty.
Aseptic toxic SKAN Isolator PSI-L for VarioSys filling machines with H2O2 SKA...FrankMartinLehmann
This presentation describes a new way of investing and working with aseptic toxic barrier isolators in a much more flexible way than was previously possible. The key point is the interchangeable filling machine equipment VarioSys for different packaging formats like vials or nested syringes that can be complemented with lyophilisation processes. Other process equipment can be mounted on L-flanges, e.g. sterility test pumps, bag fillers, stopper bag filling equipment and equipment for many other pharmaceutical processes. The compact design of the isolator inside the cleanroom, including the air handling system and switchboards, enables the pharmaceutical industry to have a fast start-up of all needed equipment. The standardization is another factor to shorten the project time schedule until the start of production for fully qualified equipment.
Eta constant temperature and humidity test chamberMark Tung
ETA Constant Temperature and Humidity Test Chamber is used to test the performance of materials in various environments. It provides a
simulated environment for testing the heat resistance, cold resistance, dry resistance and humidity resistance of materials. Suitable for
electronics, electrical appliances, communications, instrumentation, vehicles, plastic products, metals, food, chemistry, building materials,
medical, aerospace products, scientific research and other industries
This webinar will provide pesticides residue analysts with valuable information on the development and optimization of gas chromatographic separations and mass spectrometry methods for the analysis of pesticide residues in food. The expert speakers will share their knowledge in understanding the critical points of the method, assisting analysts in modifying existing methods, and understanding instrumental and software technologies with the goal of improving laboratory productivity and reducing the overall cost per sample. The results of experiments for both screening and quantification workflows, using the latest technology, will be presented.
The document summarizes information from an emissions testing webinar presented by Gregory Cole, Luke Norton, and Cameron Rapoport on February 12th, 2016. It discusses various standards and regulations for emissions testing of valves, including API 622, API 624, ISO 15848-1, and TA-Luft. The standards describe test procedures, parameters, leakage classes and allowances to qualify different types and sizes of valves. Future revisions to standards aim to reduce allowable leakage levels and eliminate re-torques.
Analysis of Volatile Organic Compounds (VOCs) in Air Using US EPA Method TO-17PerkinElmer, Inc.
EPA Method TO-171 is used to determine toxic compounds in air after they have been collected onto sorbent tubes. These tubes can either adsorb specific compounds or adsorb a broad range of compounds, quantitatively. Adsorbent tubes have many applications in the investigation of volatile organic compounds (VOCs) found in EPA Method TO-17. Examples include indoor air, fence line, stack, workplace, personal monitoring and soil gas. The type of tube used, and whether the sampling is passive or active, depends upon the need at the particular site being investigated.
This application note demonstrates that the PerkinElmer TurboMatrix™ Thermal Desorber and the PerkinElmer Clarus® SQ 8 GC/MS will meet and exceed the criteria set forth in EPA method TO-17. Detailed instrument method parameters are presented, with precision, recovery, linearity and detection limit results.
Analysis of Volatile Organic Compounds (VOCs) in Air Using US EPA Method TO-17PerkinElmer, Inc.
1) The document evaluates the use of a PerkinElmer TurboMatrix Thermal Desorber and Clarus SQ 8 GC/MS for analyzing volatile organic compounds in air samples collected using EPA Method TO-17.
2) Results showed excellent precision, linearity, and detection limits across 82 compounds, meeting or exceeding TO-17 criteria. Detection limits allowed increasing the sampling volume from 4 to 10 liters.
3) The system provides an accurate, precise, and cost-effective method for air sampling and analysis using sorbent tubes as specified in EPA Method TO-17.
Analysis of Volatile Organic Compounds (VOCs) in Air Using U.S. EPA Method TO-17PerkinElmer, Inc.
EPA Method TO-17 is used to determine toxic compounds in air after they have been collected onto sorbent tubes. These tubes can either adsorb specific compounds or adsorb a broad range of compounds, quantitatively. Adsorbent tubes have many applications in the investigation of volatile organic compounds (VOCs) found in EPA Method TO-17. Examples include indoor air, fence line, stack, workplace, personal monitoring and soil gas. The type of tube used, and whether the sampling is passive or active, depends upon the need at the particular site being investigated. This application note demonstrates that the PerkinElmer TurboMatrix™ Thermal Desorber and the PerkinElmer Clarus® SQ 8 GC/MS will meet and exceed the criteria set forth in EPA method TO-17. Detailed instrument method parameters are presented, with precision, recovery, linearity and detection limit results.
The document describes various life science products for sample preparation and analysis, including:
1) MonoSpin SPE columns for solid phase extraction of small volume samples.
2) MonoTip pipette tip SPE for efficient concentration and purification of peptide and protein samples.
3) Titansphere Phos-TiO kits for selective enrichment of phosphopeptides from protein digests.
4) MonoSpin ProA and ProG kits for rapid antibody purification using monolithic silica spin columns.
5) MonoCap high resolution LC columns for high efficiency peptide and protein separation.
The PHASER Pro is an advanced gas chromatography olfactometry (GC-O) system that allows analysts to use their sense of smell to detect and identify aroma and odor compounds separated by GC. It features a programmable transfer line to deliver separated compounds efficiently to the sniffing port, preventing heat stress or degradation. Software is included to record voice notes and assign intensities to smells detected from the column effluent at different retention times. The system provides an easy way for analysts to use their sense of smell to identify compounds in samples.
Guard columns are used to protect analytical columns in gas chromatography from contamination. A guard column is a short piece of deactivated fused silica tubing placed before the analytical column that traps contaminants from samples. When the guard column becomes dirty, a portion can be removed without affecting the analytical column's performance. Built-in guard columns integrate the guard column directly into the analytical column, avoiding potential leakage issues from separate connections.
The Titansphere Phos-TiO MP Kit from GL Sciences efficiently enriches both singly and multiply phosphorylated peptides. It employs a new protocol that fractions the peptides separately, preventing ion suppression and delivering higher recovery compared to traditional methods. The kit recovers more phosphorylated peptides in total, with singly phosphorylated peptides eluting in acidic buffer and multiply phosphorylated peptides eluting in basic buffer.
The document discusses PEEK columns for HPLC and LC/MS. PEEK columns offer better chemical inertness compared to stainless steel columns. This reduces issues caused by metallic impurities interacting with analytes like phosphate groups. PEEK columns are available with InertSustain and Inertsil packing materials in particle sizes from 1.9 μm to 10 μm. They provide excellent peak shapes without analyte adsorption. An ordering guide provides the catalog numbers for various PEEK column configurations.
Pyrolysis Gas Chromatography. PY-GCMS Geert Alkema
Pyrolysis is the breaking apart of chemical bonds through thermal energy. Analytical pyrolysis introduces solid and high molecular weight samples to a gas chromatograph by breaking them down. It is used to minimize sample preparation and analyze whole samples. Applications include forensics, polymers, and microorganisms. Samples are placed in a quartz tube or vial and pyrolyzed. Liquid samples can also be injected and pyrolyzed. The OPTIC inlet allows for fast, high temperature pyrolysis for both solid and liquid samples.
Unlock the Future of Search with MongoDB Atlas_ Vector Search Unleashed.pdfMalak Abu Hammad
Discover how MongoDB Atlas and vector search technology can revolutionize your application's search capabilities. This comprehensive presentation covers:
* What is Vector Search?
* Importance and benefits of vector search
* Practical use cases across various industries
* Step-by-step implementation guide
* Live demos with code snippets
* Enhancing LLM capabilities with vector search
* Best practices and optimization strategies
Perfect for developers, AI enthusiasts, and tech leaders. Learn how to leverage MongoDB Atlas to deliver highly relevant, context-aware search results, transforming your data retrieval process. Stay ahead in tech innovation and maximize the potential of your applications.
#MongoDB #VectorSearch #AI #SemanticSearch #TechInnovation #DataScience #LLM #MachineLearning #SearchTechnology
Enchancing adoption of Open Source Libraries. A case study on Albumentations.AIVladimir Iglovikov, Ph.D.
Presented by Vladimir Iglovikov:
- https://www.linkedin.com/in/iglovikov/
- https://x.com/viglovikov
- https://www.instagram.com/ternaus/
This presentation delves into the journey of Albumentations.ai, a highly successful open-source library for data augmentation.
Created out of a necessity for superior performance in Kaggle competitions, Albumentations has grown to become a widely used tool among data scientists and machine learning practitioners.
This case study covers various aspects, including:
People: The contributors and community that have supported Albumentations.
Metrics: The success indicators such as downloads, daily active users, GitHub stars, and financial contributions.
Challenges: The hurdles in monetizing open-source projects and measuring user engagement.
Development Practices: Best practices for creating, maintaining, and scaling open-source libraries, including code hygiene, CI/CD, and fast iteration.
Community Building: Strategies for making adoption easy, iterating quickly, and fostering a vibrant, engaged community.
Marketing: Both online and offline marketing tactics, focusing on real, impactful interactions and collaborations.
Mental Health: Maintaining balance and not feeling pressured by user demands.
Key insights include the importance of automation, making the adoption process seamless, and leveraging offline interactions for marketing. The presentation also emphasizes the need for continuous small improvements and building a friendly, inclusive community that contributes to the project's growth.
Vladimir Iglovikov brings his extensive experience as a Kaggle Grandmaster, ex-Staff ML Engineer at Lyft, sharing valuable lessons and practical advice for anyone looking to enhance the adoption of their open-source projects.
Explore more about Albumentations and join the community at:
GitHub: https://github.com/albumentations-team/albumentations
Website: https://albumentations.ai/
LinkedIn: https://www.linkedin.com/company/100504475
Twitter: https://x.com/albumentations
Building RAG with self-deployed Milvus vector database and Snowpark Container...Zilliz
This talk will give hands-on advice on building RAG applications with an open-source Milvus database deployed as a docker container. We will also introduce the integration of Milvus with Snowpark Container Services.
Securing your Kubernetes cluster_ a step-by-step guide to success !KatiaHIMEUR1
Today, after several years of existence, an extremely active community and an ultra-dynamic ecosystem, Kubernetes has established itself as the de facto standard in container orchestration. Thanks to a wide range of managed services, it has never been so easy to set up a ready-to-use Kubernetes cluster.
However, this ease of use means that the subject of security in Kubernetes is often left for later, or even neglected. This exposes companies to significant risks.
In this talk, I'll show you step-by-step how to secure your Kubernetes cluster for greater peace of mind and reliability.
Why You Should Replace Windows 11 with Nitrux Linux 3.5.0 for enhanced perfor...SOFTTECHHUB
The choice of an operating system plays a pivotal role in shaping our computing experience. For decades, Microsoft's Windows has dominated the market, offering a familiar and widely adopted platform for personal and professional use. However, as technological advancements continue to push the boundaries of innovation, alternative operating systems have emerged, challenging the status quo and offering users a fresh perspective on computing.
One such alternative that has garnered significant attention and acclaim is Nitrux Linux 3.5.0, a sleek, powerful, and user-friendly Linux distribution that promises to redefine the way we interact with our devices. With its focus on performance, security, and customization, Nitrux Linux presents a compelling case for those seeking to break free from the constraints of proprietary software and embrace the freedom and flexibility of open-source computing.
In the rapidly evolving landscape of technologies, XML continues to play a vital role in structuring, storing, and transporting data across diverse systems. The recent advancements in artificial intelligence (AI) present new methodologies for enhancing XML development workflows, introducing efficiency, automation, and intelligent capabilities. This presentation will outline the scope and perspective of utilizing AI in XML development. The potential benefits and the possible pitfalls will be highlighted, providing a balanced view of the subject.
We will explore the capabilities of AI in understanding XML markup languages and autonomously creating structured XML content. Additionally, we will examine the capacity of AI to enrich plain text with appropriate XML markup. Practical examples and methodological guidelines will be provided to elucidate how AI can be effectively prompted to interpret and generate accurate XML markup.
Further emphasis will be placed on the role of AI in developing XSLT, or schemas such as XSD and Schematron. We will address the techniques and strategies adopted to create prompts for generating code, explaining code, or refactoring the code, and the results achieved.
The discussion will extend to how AI can be used to transform XML content. In particular, the focus will be on the use of AI XPath extension functions in XSLT, Schematron, Schematron Quick Fixes, or for XML content refactoring.
The presentation aims to deliver a comprehensive overview of AI usage in XML development, providing attendees with the necessary knowledge to make informed decisions. Whether you’re at the early stages of adopting AI or considering integrating it in advanced XML development, this presentation will cover all levels of expertise.
By highlighting the potential advantages and challenges of integrating AI with XML development tools and languages, the presentation seeks to inspire thoughtful conversation around the future of XML development. We’ll not only delve into the technical aspects of AI-powered XML development but also discuss practical implications and possible future directions.
Unlocking Productivity: Leveraging the Potential of Copilot in Microsoft 365, a presentation by Christoforos Vlachos, Senior Solutions Manager – Modern Workplace, Uni Systems
Alt. GDG Cloud Southlake #33: Boule & Rebala: Effective AppSec in SDLC using ...James Anderson
Effective Application Security in Software Delivery lifecycle using Deployment Firewall and DBOM
The modern software delivery process (or the CI/CD process) includes many tools, distributed teams, open-source code, and cloud platforms. Constant focus on speed to release software to market, along with the traditional slow and manual security checks has caused gaps in continuous security as an important piece in the software supply chain. Today organizations feel more susceptible to external and internal cyber threats due to the vast attack surface in their applications supply chain and the lack of end-to-end governance and risk management.
The software team must secure its software delivery process to avoid vulnerability and security breaches. This needs to be achieved with existing tool chains and without extensive rework of the delivery processes. This talk will present strategies and techniques for providing visibility into the true risk of the existing vulnerabilities, preventing the introduction of security issues in the software, resolving vulnerabilities in production environments quickly, and capturing the deployment bill of materials (DBOM).
Speakers:
Bob Boule
Robert Boule is a technology enthusiast with PASSION for technology and making things work along with a knack for helping others understand how things work. He comes with around 20 years of solution engineering experience in application security, software continuous delivery, and SaaS platforms. He is known for his dynamic presentations in CI/CD and application security integrated in software delivery lifecycle.
Gopinath Rebala
Gopinath Rebala is the CTO of OpsMx, where he has overall responsibility for the machine learning and data processing architectures for Secure Software Delivery. Gopi also has a strong connection with our customers, leading design and architecture for strategic implementations. Gopi is a frequent speaker and well-known leader in continuous delivery and integrating security into software delivery.
Removing Uninteresting Bytes in Software FuzzingAftab Hussain
Imagine a world where software fuzzing, the process of mutating bytes in test seeds to uncover hidden and erroneous program behaviors, becomes faster and more effective. A lot depends on the initial seeds, which can significantly dictate the trajectory of a fuzzing campaign, particularly in terms of how long it takes to uncover interesting behaviour in your code. We introduce DIAR, a technique designed to speedup fuzzing campaigns by pinpointing and eliminating those uninteresting bytes in the seeds. Picture this: instead of wasting valuable resources on meaningless mutations in large, bloated seeds, DIAR removes the unnecessary bytes, streamlining the entire process.
In this work, we equipped AFL, a popular fuzzer, with DIAR and examined two critical Linux libraries -- Libxml's xmllint, a tool for parsing xml documents, and Binutil's readelf, an essential debugging and security analysis command-line tool used to display detailed information about ELF (Executable and Linkable Format). Our preliminary results show that AFL+DIAR does not only discover new paths more quickly but also achieves higher coverage overall. This work thus showcases how starting with lean and optimized seeds can lead to faster, more comprehensive fuzzing campaigns -- and DIAR helps you find such seeds.
- These are slides of the talk given at IEEE International Conference on Software Testing Verification and Validation Workshop, ICSTW 2022.
Dr. Sean Tan, Head of Data Science, Changi Airport Group
Discover how Changi Airport Group (CAG) leverages graph technologies and generative AI to revolutionize their search capabilities. This session delves into the unique search needs of CAG’s diverse passengers and customers, showcasing how graph data structures enhance the accuracy and relevance of AI-generated search results, mitigating the risk of “hallucinations” and improving the overall customer journey.
Observability Concepts EVERY Developer Should Know -- DeveloperWeek Europe.pdfPaige Cruz
Monitoring and observability aren’t traditionally found in software curriculums and many of us cobble this knowledge together from whatever vendor or ecosystem we were first introduced to and whatever is a part of your current company’s observability stack.
While the dev and ops silo continues to crumble….many organizations still relegate monitoring & observability as the purview of ops, infra and SRE teams. This is a mistake - achieving a highly observable system requires collaboration up and down the stack.
I, a former op, would like to extend an invitation to all application developers to join the observability party will share these foundational concepts to build on:
3. 4th
generation of the world’s most powerful inlet for Gas Chromatography
OPTIC Multimode Inlet changes your
GC or GCMS into a flexible and multi-
usable system with increased
application range and detection limits
4. One Inlet, More Analytical Options
OPTIC Inlet is much more than any other Inlet
6. Your GCMS would be able to handle:
Cold and Hot Split/Splitless injections
Pyrolysis (liquid and solid samples)
Large Volume Injections
In-liner derivatisation
LC-GC coupling possibility
Headspace
CryoFocusing on the column
Thermal Desorbtion
On-Column injections
Automatic liner exchange
7. Direct heating technology
Low thermal mass
Septum or Merlin Seal
Septum purge line
Carrier line
Split line
High power
O-ring
Liner I.D. = 3.4mm
Oven wall
Cooling by Air, CO2, LN2
computer modeled temp. profile Max temp.: 600˚C
Max ramp : 60˚C/sec
Thermocouple
Solvent Monitor
patented
8. Benefits of OPTIC Inlet
Real Septum Purge flow
Different septa ca be used: Plug type
Disk type
Merlin Microseal ™
Metal column ferrule can be used
9. Benefits of OPTIC Inlet
Low thermal mass >:
High temperatures (up to 600°C)
Fast ramp rates (up to 60°C/sec)
Fast cool down time
Relatively large liner diameter (3.4 mm i.d.) >:
150µl At-Once LVI
Direct Thermal Desorbtion (DTD)
Difficult Matrix Introduction (DMI)
In-Liner derivatisation
Solid and Liquid sample Pyrolysis
Best temperature profile >:
good transfer of high molecular components
11. No discrimination
No evaporate from the syringe needle
More control over the true injected volume
Less thermal degradation (Less problems with thermally labile
compounds degrade due to the thermal stress upon injection)
Better reproducibility with SPME
Very good reproducibility, also using CombiPAL
Benefits of cold injections
14. Benefits of Large Volume Injection
Sample vol 1000ml 10ml 10ml
Elution vol. 2000µl 2000µl 200µl
Mass in 1µl 5pg 0.05pg 0.5pg
Injection vol. 1µl 100µl 100µl
Mass injected 5pg 5pg 50pg
Less sample and solvent is needed and
finally more is arrived at the detector.
Less sample and solvent is needed and
finally more is arrived at the detector.
15. LVI; Why so good with OPTIC ?
(s)10 20 30 40 50
30 o
C
15
0
-15
Temp.
In the liner
C5
C6
EtOAc
MeOH
Good evaporate cooling effect because inlet
body has a very low thermal mass
16. LVI Solvent Vent Monitor Function
in OPTIC
Sensor
Output
Time
Inject Vent valve closes
Sensor in split lineSensor in split line
(standard available)(standard available)
17. Difficult Matrix Introduction (DMI) Raw sample or dirty
extract is put into micro vial and placed directly into liner
In-injector thermal desorption or pyrolysis of liquid or solid
samples
What is Difficult Matrix Introduction (DMI)?
18. d)
OP TIC
Injector
e)
Ca rrier In
Split Flow
Column Flow
Transport liner with sample into OPTIC inlet
Seal and purge the inlet. Apply selective exclusion principle for the analysis:
First temperature increased to just below BP of solvent
Solvent is vented through split line
Temperature and pressure ramped up to achieve selective transfer
Dispose microvial after analysis, clean and re-use liner
DMI step by step:DMI step by step:
What is Difficult Matrix Introduction (DMI)?
19. Pyrolysis Liquid
T (°C)
t (min)
injection solvent
elimination
pyrolysis
600
40
0 1 5
Liquid injection: simple to automate pyrolysis
Sample inlet
Vapour outlet - Temperature program to 600°C
- Fast ramp rate (60°C/sec)
20. Pyrolysis Solid
- Sample in micro vial
- Fast ramp rate (60°C/sec)
- Temperature program to 600°C
- Multi-Shot Pyrolysis possible
- Evolved Gas Analysis (EGA) possible
21. On-Column solution
No press fit
No liner
No modification to OPTIC
Inlet
Easy installation
Real On-Column
New type Merlin Microseal
can be used
22. CryoTrap Option
Different ramp rates for same component.
60°C/sec
30°C/sec
10°C/sec
Cryogenic cold trapping is frequently used for narrowing the
chromatographic band and improving the detection limit.
OPTIC-4 Cryotrap is the fastest heating trap > resulting in sharp peaks
23. ATAS GL – Thermal Desoption Solution (TDS)
Main points:Main points:
- Direct heatingDirect heating
- Trapping on the column (one desorption from liner to column)Trapping on the column (one desorption from liner to column)
- No dead volumeNo dead volume
- Real septum purgeReal septum purge
- Real Splitless transfer from sample to columnReal Splitless transfer from sample to column
24. LINERS
Fritted liner, frit on 15 mm
liner for split injections (single necked)
Empty liner for splitless injections
Baffled splitless liner
Fritted liner on 20 mm with single taper
“A” type packed liner for large volume injections
“8270” sintered liner for large volume injections
Sintered glass liner with taper
LINEX DMI Liner
DMI Liner Insert (micro vial)
25. OPTIC Injection Modes
Combined TD / Pyrolysis
Injection port reactor
Micro Headspace / Micro vial
SPME-GC
SPE-GC
TCT-GC
GPC-GC
LC-GC
Comprehensive LC x GC
Automatically changing liners
Hot split injection
Hot splitless injection
Cold split injection
Cold splitless injection
On-column injection
Large Volume Injection
Gas enrichment
Direct thermal desorption
Pyrolysis (in liner)
Headspace