The document provides guidelines for laboratory safety. It outlines personal protective equipment that should be worn, such as lab coats, gloves, and enclosed shoes. It describes emergency procedures for incidents involving splashes to the eyes, body, or spills. Good laboratory conduct is also discussed, including following instructions, restraining loose clothing and jewelry, and not eating/drinking in the lab. Proper use and handling of microscopes is explained.

1. BASICS OF LABORATORY
SAFETY
Introduction to
Microbiology Laboratory
Donna C. Sullivan, PhD

2. Before Beginning Any Laboratory
Exercise
 Know the dangers
associated with all
materials you will use in
this laboratory exercise
 Know the proper way of
using all the materials
provided for the
exercise
 Know all the protective
measures you must use
during the exercise

3. Personal Protection
 Wear an apron or lab
coat in the proper
manner
 Wear gloves in the
proper manner
 Wear a face shield in
the proper manner

4. Personal Protection
 Tie long hair neatly at
the back of the neck
 Wear shoes that
enclose your entire feet

5. Emergency Procedures
 Know where all exit
doors are located
 If a reagent splashes
onto your face or eyes,
wash your face at the
eyewash station for
several minutes

6. Emergency Procedures
 If an irritating or harmful
reagent splashes onto
your body, use the
emergency shower to
wash your body for
several minutes
 Know where the fire
extinguisher and fire
blanket are located

7. Emergency Procedures
 If a reagent spills onto the workbench or
floor, notify the instructor immediately for
cleanup instructions.
 If a glass container gets broken, notify the
instructor immediately for cleanup
instructions.
 Report all accidents, injuries and close calls
immediately to the instructor.

8. Good Laboratory Conduct
 Follow all instructions
 Do not play in the
laboratory
 Do not use any
laboratory equipment
until you are told to do
so
 Do not perform any
laboratory procedure
until you are told to do
so

9. Good Laboratory Conduct
 Do not bring food,
beverages or tobacco
products into the
laboratory
 Do not apply cosmetics
in the laboratory
 Do not eat or drink in
the laboratory
 Restrain loose clothing
and jewelry

10. Before You Leave The Laboratory
 Clean your workbench with
disinfectant at the beginning
and at the end of the
laboratory exercise
 Leave all equipment,
samples and reagents in the
lab
 Leave your lab coat (apron)
in the lab
 Wash your hands with soap
and water

11. COMPOUND MICROSCOPE

12. COMPOUND MICROSCOPE

13. COMPOUND MICROSCOPE

14. COMPOUND MICROSCOPE

15. Compound Light Microscope

16. Using the Microscope
 To carry the microscope grasp the
microscopes arm with one hand. Place your
other hand under the base.
 Place the microscope on a table with the arm
toward you.
 Turn the coarse adjustment knob to raise the
body tube.
 Revolve the nosepiece until the low-power
objective lens clicks into place.

17. Total Magnification

18. PREPARING WET MOUNTS

19. PREPARING WET MOUNT

20. ADJUSTING MICROSCOPE