cultivation, isolation,purification and characterization of microorganism Amjad Afridi
Microorganisms can be studied through cultivation, isolation, purification, and characterization techniques. Samples are collected from various sources and streaked onto agar plates using aseptic technique to prevent contamination. Single colonies are picked and restreaked to obtain a pure culture of a single microbial species. The isolated microbes are then characterized through morphological examination, staining techniques, and biochemical tests to identify the microorganism.
General methods of studying microorganisms cultivation, isolation,purificatio...Amjad Afridi
Microorganisms can be studied through cultivation, isolation, purification, and characterization techniques. Samples are collected from various sources and streaked onto agar plates using aseptic technique to prevent contamination. Single colonies are then purified by streaking and incubating to obtain a pure culture of a single microbial species. The isolated microbe can be characterized through morphological analysis under a microscope, biochemical testing of metabolic activities, and other identification methods. This allows scientists to determine the type of microorganism being studied.
This document discusses various types of culture media and biochemical tests used in microbiology. It provides details on basal, enriched, selective, indicator, transport, and storage media. It also describes several common biochemical tests including the indole, triple sugar iron, citrate, urea, phenylpyruvic acid, mannitol motility, and gram staining tests. The document explains the procedures and expected results for each of these tests which are used to identify and differentiate between bacterial species.
Bacteria Classification By Gram Staining EssayChristy Hunt
Bizzozero staining procedure involves classifying tissues into three categories based on their mitotic activity as seen under the microscope: category I tissues with low mitotic activity, category II tissues with moderate mitotic activity, and category III tissues with high mitotic activity. The staining procedure uses proliferating cell nuclear antigen (PCNA) to label proliferating cells and support Bizzozero's 1894 tissue classification system based on mitotic index determined by examining hematoxylin and eosin stained slides under the microscope. The experiment aims to evaluate if PCNA staining agrees with Bizzozero's original tissue categorization into
Outline:
1. Difference between Light microscopy and
electron microscopy
2. Decribe methods for the isolation of
microorganisms in pure culture
3. Techniques for studying live bacteria
4. Distinguish between a simple stain and a
differential stain and give examples
5. Identify steps in the Gram stain procedure
6. List the major categories of microbial characteristics
used to identify microorganisms. Explain why some of
these give more specific info for identification than others
cultivation, isolation,purification and characterization of microorganism Amjad Afridi
Microorganisms can be studied through cultivation, isolation, purification, and characterization techniques. Samples are collected from various sources and streaked onto agar plates using aseptic technique to prevent contamination. Single colonies are picked and restreaked to obtain a pure culture of a single microbial species. The isolated microbes are then characterized through morphological examination, staining techniques, and biochemical tests to identify the microorganism.
General methods of studying microorganisms cultivation, isolation,purificatio...Amjad Afridi
Microorganisms can be studied through cultivation, isolation, purification, and characterization techniques. Samples are collected from various sources and streaked onto agar plates using aseptic technique to prevent contamination. Single colonies are then purified by streaking and incubating to obtain a pure culture of a single microbial species. The isolated microbe can be characterized through morphological analysis under a microscope, biochemical testing of metabolic activities, and other identification methods. This allows scientists to determine the type of microorganism being studied.
This document discusses various types of culture media and biochemical tests used in microbiology. It provides details on basal, enriched, selective, indicator, transport, and storage media. It also describes several common biochemical tests including the indole, triple sugar iron, citrate, urea, phenylpyruvic acid, mannitol motility, and gram staining tests. The document explains the procedures and expected results for each of these tests which are used to identify and differentiate between bacterial species.
Bacteria Classification By Gram Staining EssayChristy Hunt
Bizzozero staining procedure involves classifying tissues into three categories based on their mitotic activity as seen under the microscope: category I tissues with low mitotic activity, category II tissues with moderate mitotic activity, and category III tissues with high mitotic activity. The staining procedure uses proliferating cell nuclear antigen (PCNA) to label proliferating cells and support Bizzozero's 1894 tissue classification system based on mitotic index determined by examining hematoxylin and eosin stained slides under the microscope. The experiment aims to evaluate if PCNA staining agrees with Bizzozero's original tissue categorization into
Outline:
1. Difference between Light microscopy and
electron microscopy
2. Decribe methods for the isolation of
microorganisms in pure culture
3. Techniques for studying live bacteria
4. Distinguish between a simple stain and a
differential stain and give examples
5. Identify steps in the Gram stain procedure
6. List the major categories of microbial characteristics
used to identify microorganisms. Explain why some of
these give more specific info for identification than others
The document provides an overview of microbiology and laboratory techniques used for the identification of microorganisms. It discusses the types of microorganisms including bacteria, viruses, and fungi. It also describes various sterilization and staining methods used in the laboratory such as Gram staining, acid-fast staining, and biochemical tests to identify bacteria. These techniques are important tools for classifying bacteria and determining the cause of infectious diseases.
Isolation of bacteria is an important step in diagnosing bacterial infections. There are various methods used for isolating bacteria, including culture methods using solid or liquid media, and non-culture methods like PCR. For culture, appropriate specimens are collected and transported to the lab, where microscopy is first performed to view bacteria. The specimens are then plated on selective and non-selective media and incubated under optimal conditions for bacterial growth. Isolates are identified based on colony characteristics. Automated systems can also be used to more rapidly detect bacterial growth through liquid culture.
Isolation of bacteria is an important step in diagnosing bacterial infections. There are various methods used for isolating bacteria, including culture methods using solid or liquid media, and non-culture methods like PCR. For culture, appropriate specimens are collected and transported to the lab, where microscopy is first performed to view bacteria. The specimens are then plated on selective and non-selective media and incubated under optimal conditions for bacterial growth. Isolates are identified based on colony characteristics. Automated systems can also be used to more rapidly detect bacterial growth through liquid culture.
Physical Factors: Temperature,Physical Factors: pH of the Extracellular Envir...Md Azizul Haque
The document describes an experiment to determine the pH requirements of microorganisms. It explains that microbial growth is influenced by environmental pH, with each species having an optimal pH range for growth, usually between pH 4-9 for bacteria. The experiment aims to observe the growth of different microbes at various pH levels to determine their pH classifications as acidophilic, neutralophilic, or alkalophilic.
The document describes experiments conducted to isolate, characterize, and identify an unknown species of bacteria collected from soil in Flagstaff, Arizona. A series of tests were performed on the isolated bacteria, including Gram staining, endospore staining, catalase testing, carbohydrate testing, and more. Results of the tests were used to compare the unknown bacteria to known species in Bergey's Manual of Systematic Bacteriology in order to identify the bacteria. Preliminary analysis indicated the bacteria were adapted to the alkaline soil and climate conditions of Flagstaff's high altitude location.
The student was given an unknown mixed culture and used quadrant streaking to isolate a pure colony. A Gram stain showed the isolated bacteria was Gram positive. Motility tests found it was non-motile. A series of metabolic tests for properties like citrate utilization, carbohydrate fermentation, and catalase presence were done. By comparing the results to database entries, the unknown bacteria was identified as Staphylococcus.
Microbial biotechnology unit 1 [B.Sc Biotechnology].pptxHariniRaja4
This document summarizes key aspects of isolating, screening, and maintaining industrially important microbes. It discusses isolating microbes from various environmental sources and using primary and secondary screening to identify microbes that can produce desired products. Some important characteristics of producer strains are described. Techniques for maintaining microbes include periodic transfer, overlaying with oil, saline suspension, storage in soil or silica gel, lyophilization, and storage in liquid nitrogen. Strain improvement methods to increase yield or modify characteristics are also summarized, such as mutagenesis, optimizing growth conditions and nutrition, and site-directed mutation.
Fa1zanS: Methods of studying biochemical properties of microorganismFaizan Siddiqui
Methods of studying biochemical properties of microorganism, how they are cultured in laboratory and how they play key role in maintaining the equilibrium of our environment, their role in energy production, agriculture, ecological balance. Media: Providing Nutrients in the Laboratory. Incubation, Inspection, and Identification. Methods of cultivation
Staining techniques are used in microbiology to identify bacteria under a microscope. There are several types of staining including simple staining with one dye, Gram staining which differentiates bacteria as Gram-positive or Gram-negative based on cell wall structure, and acid-fast staining used to identify Mycobacterium species. Biochemical tests such as IMViC (Indole, Methyl Red, Voges-Proskauer, Citrate) are also used to identify bacteria based on their metabolic reactions and products.
Characterization of Bacteria Isolated from Tropical Soils of Puerto Rico ramoncolon7
This document summarizes a student research project that aimed to characterize bacteria isolated from soils in Puerto Rico. Soil samples were collected and bacteria were isolated on agar plates before being purified through restreaking. Gram staining identified the bacteria as either gram-positive or gram-negative. Genomic DNA was extracted and amplified via PCR. However, when tested on indicator plates, the isolated bacteria did not show any antibiotic production against E. coli or M. luteus as hypothesized. While full characterization was not completed due to time constraints, the student plans future work identifying the bacteria through DNA sequencing.
Bacteriology physiology 1-mbbs-y2-5-oct2011---2Lawrence James
The document discusses bacterial physiology and growth. It covers the following key points:
1) Bacteria require nutrients like carbon, nitrogen, oxygen, hydrogen, sulfur, and phosphorus for growth. They also require growth factors and microelements.
2) Environmental factors that affect bacterial growth include temperature, pH, oxygen availability, and water availability.
3) Bacteria are commonly grown in the laboratory using solid or liquid culture media, which must be sterilized to obtain a pure culture and prevent contamination.
4) The bacterial growth curve consists of four phases: lag, log (exponential), stationary, and death phases. The bacteria acclimate, multiply rapidly, stop growing due to lack of nutrients,
The document discusses bacterial physiology and growth. It covers the following key points:
1) Bacteria require nutrients like carbon, nitrogen, oxygen, hydrogen, sulfur, and phosphorus for growth. They also require growth factors and microelements.
2) Environmental factors that affect bacterial growth include temperature, pH, oxygen availability, and water availability.
3) Bacteria are commonly grown in the laboratory using solid or liquid culture media, which must be sterilized to obtain a pure culture and prevent contamination.
4) The bacterial growth curve consists of four phases: lag, log (exponential), stationary, and death phases. The bacteria acclimate, multiply rapidly, stop growing due to lack of nutrients,
Bacteriology physiology 1-mbbs-y2-5-oct2011---2Lawrence James
The document discusses bacterial physiology and growth. It covers the following key points:
1) Bacteria require nutrients like carbon, nitrogen, oxygen, hydrogen, sulfur, and phosphorus for growth. They also require growth factors and microelements.
2) Environmental factors that affect bacterial growth include temperature, pH, oxygen availability, and water availability.
3) Bacteria are commonly grown in the laboratory using solid or liquid culture media, which must be sterilized to obtain a pure culture and prevent contamination.
4) The bacterial growth curve consists of four phases: lag, log (exponential), stationary, and death phases. The bacteria acclimate, multiply rapidly, stop growing due to lack of nutrients,
This research aimed to characterize bacteria isolated from soils in Puerto Rico. Two soil samples were collected and various tests were performed on the isolated bacteria, including Gram staining, DNA purification and amplification, and testing for antibiotic production. One isolated bacterium (S15UPRC-RISEAMGP30SP01A1) was identified as a gram-positive cocci resembling Staphylococcus and did not produce antibiotics. The other (S15UPRC-RISERBCR30P01A2) was a gram-positive Bacillus but did not produce antibiotics. Neither bacterium could be fully characterized due to negative PCR results, failing to confirm the hypothesis of positive antibiotic production. Further tests are needed to fully characterize the
The document discusses general principles for diagnosing infectious diseases, including:
1. Physical examination, clinical diagnosis, and epidemiological assessment help identify possible pathogens.
2. Laboratory tests are needed to confirm the causative agent, including microscopic examination, culture-based methods, and immunological or molecular detection techniques.
3. Proper specimen collection, transport, and timing are important for accurate diagnostic results.
The document discusses various methods for diagnosing important bacterial diseases through laboratory examination. Effective diagnosis allows for timely treatment and control measures. Key methods discussed include microscopy, culture techniques, biochemical reactions, serological identification, and molecular diagnosis. Microscopy can identify bacterial morphology and staining properties. Culture techniques isolate bacteria on selective media and examine colony characteristics. Biochemical tests identify metabolic properties. Serology detects bacterial antigens and antibodies. Molecular methods like PCR and sequencing provide sensitive, specific identification and can detect non-culturable bacteria. Together, these diagnostic methods allow clinicians to initiate appropriate treatment and control of bacterial outbreaks.
The document provides information on the diagnosis of important bacterial diseases. It discusses the importance of quick diagnostic results for effective treatment during disease outbreaks. It covers the types and activities of various antimicrobial classes against different bacteria and microorganisms. It also describes the scope of bacterial infections, types of bacteria, why diagnosis is needed, recommended diagnostics for various diseases, steps in diagnosis, prerequisites for laboratory examination including appropriate sample collection and transport, and various microbiological diagnostic techniques like microscopy, culture, and biochemical identification.
This document provides an overview of wound healing, its functions, stages, mechanisms, factors affecting it, and complications.
A wound is a break in the integrity of the skin or tissues, which may be associated with disruption of the structure and function.
Healing is the body’s response to injury in an attempt to restore normal structure and functions.
Healing can occur in two ways: Regeneration and Repair
There are 4 phases of wound healing: hemostasis, inflammation, proliferation, and remodeling. This document also describes the mechanism of wound healing. Factors that affect healing include infection, uncontrolled diabetes, poor nutrition, age, anemia, the presence of foreign bodies, etc.
Complications of wound healing like infection, hyperpigmentation of scar, contractures, and keloid formation.
How to Add Chatter in the odoo 17 ERP ModuleCeline George
In Odoo, the chatter is like a chat tool that helps you work together on records. You can leave notes and track things, making it easier to talk with your team and partners. Inside chatter, all communication history, activity, and changes will be displayed.
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The document provides an overview of microbiology and laboratory techniques used for the identification of microorganisms. It discusses the types of microorganisms including bacteria, viruses, and fungi. It also describes various sterilization and staining methods used in the laboratory such as Gram staining, acid-fast staining, and biochemical tests to identify bacteria. These techniques are important tools for classifying bacteria and determining the cause of infectious diseases.
Isolation of bacteria is an important step in diagnosing bacterial infections. There are various methods used for isolating bacteria, including culture methods using solid or liquid media, and non-culture methods like PCR. For culture, appropriate specimens are collected and transported to the lab, where microscopy is first performed to view bacteria. The specimens are then plated on selective and non-selective media and incubated under optimal conditions for bacterial growth. Isolates are identified based on colony characteristics. Automated systems can also be used to more rapidly detect bacterial growth through liquid culture.
Isolation of bacteria is an important step in diagnosing bacterial infections. There are various methods used for isolating bacteria, including culture methods using solid or liquid media, and non-culture methods like PCR. For culture, appropriate specimens are collected and transported to the lab, where microscopy is first performed to view bacteria. The specimens are then plated on selective and non-selective media and incubated under optimal conditions for bacterial growth. Isolates are identified based on colony characteristics. Automated systems can also be used to more rapidly detect bacterial growth through liquid culture.
Physical Factors: Temperature,Physical Factors: pH of the Extracellular Envir...Md Azizul Haque
The document describes an experiment to determine the pH requirements of microorganisms. It explains that microbial growth is influenced by environmental pH, with each species having an optimal pH range for growth, usually between pH 4-9 for bacteria. The experiment aims to observe the growth of different microbes at various pH levels to determine their pH classifications as acidophilic, neutralophilic, or alkalophilic.
The document describes experiments conducted to isolate, characterize, and identify an unknown species of bacteria collected from soil in Flagstaff, Arizona. A series of tests were performed on the isolated bacteria, including Gram staining, endospore staining, catalase testing, carbohydrate testing, and more. Results of the tests were used to compare the unknown bacteria to known species in Bergey's Manual of Systematic Bacteriology in order to identify the bacteria. Preliminary analysis indicated the bacteria were adapted to the alkaline soil and climate conditions of Flagstaff's high altitude location.
The student was given an unknown mixed culture and used quadrant streaking to isolate a pure colony. A Gram stain showed the isolated bacteria was Gram positive. Motility tests found it was non-motile. A series of metabolic tests for properties like citrate utilization, carbohydrate fermentation, and catalase presence were done. By comparing the results to database entries, the unknown bacteria was identified as Staphylococcus.
Microbial biotechnology unit 1 [B.Sc Biotechnology].pptxHariniRaja4
This document summarizes key aspects of isolating, screening, and maintaining industrially important microbes. It discusses isolating microbes from various environmental sources and using primary and secondary screening to identify microbes that can produce desired products. Some important characteristics of producer strains are described. Techniques for maintaining microbes include periodic transfer, overlaying with oil, saline suspension, storage in soil or silica gel, lyophilization, and storage in liquid nitrogen. Strain improvement methods to increase yield or modify characteristics are also summarized, such as mutagenesis, optimizing growth conditions and nutrition, and site-directed mutation.
Fa1zanS: Methods of studying biochemical properties of microorganismFaizan Siddiqui
Methods of studying biochemical properties of microorganism, how they are cultured in laboratory and how they play key role in maintaining the equilibrium of our environment, their role in energy production, agriculture, ecological balance. Media: Providing Nutrients in the Laboratory. Incubation, Inspection, and Identification. Methods of cultivation
Staining techniques are used in microbiology to identify bacteria under a microscope. There are several types of staining including simple staining with one dye, Gram staining which differentiates bacteria as Gram-positive or Gram-negative based on cell wall structure, and acid-fast staining used to identify Mycobacterium species. Biochemical tests such as IMViC (Indole, Methyl Red, Voges-Proskauer, Citrate) are also used to identify bacteria based on their metabolic reactions and products.
Characterization of Bacteria Isolated from Tropical Soils of Puerto Rico ramoncolon7
This document summarizes a student research project that aimed to characterize bacteria isolated from soils in Puerto Rico. Soil samples were collected and bacteria were isolated on agar plates before being purified through restreaking. Gram staining identified the bacteria as either gram-positive or gram-negative. Genomic DNA was extracted and amplified via PCR. However, when tested on indicator plates, the isolated bacteria did not show any antibiotic production against E. coli or M. luteus as hypothesized. While full characterization was not completed due to time constraints, the student plans future work identifying the bacteria through DNA sequencing.
Bacteriology physiology 1-mbbs-y2-5-oct2011---2Lawrence James
The document discusses bacterial physiology and growth. It covers the following key points:
1) Bacteria require nutrients like carbon, nitrogen, oxygen, hydrogen, sulfur, and phosphorus for growth. They also require growth factors and microelements.
2) Environmental factors that affect bacterial growth include temperature, pH, oxygen availability, and water availability.
3) Bacteria are commonly grown in the laboratory using solid or liquid culture media, which must be sterilized to obtain a pure culture and prevent contamination.
4) The bacterial growth curve consists of four phases: lag, log (exponential), stationary, and death phases. The bacteria acclimate, multiply rapidly, stop growing due to lack of nutrients,
The document discusses bacterial physiology and growth. It covers the following key points:
1) Bacteria require nutrients like carbon, nitrogen, oxygen, hydrogen, sulfur, and phosphorus for growth. They also require growth factors and microelements.
2) Environmental factors that affect bacterial growth include temperature, pH, oxygen availability, and water availability.
3) Bacteria are commonly grown in the laboratory using solid or liquid culture media, which must be sterilized to obtain a pure culture and prevent contamination.
4) The bacterial growth curve consists of four phases: lag, log (exponential), stationary, and death phases. The bacteria acclimate, multiply rapidly, stop growing due to lack of nutrients,
Bacteriology physiology 1-mbbs-y2-5-oct2011---2Lawrence James
The document discusses bacterial physiology and growth. It covers the following key points:
1) Bacteria require nutrients like carbon, nitrogen, oxygen, hydrogen, sulfur, and phosphorus for growth. They also require growth factors and microelements.
2) Environmental factors that affect bacterial growth include temperature, pH, oxygen availability, and water availability.
3) Bacteria are commonly grown in the laboratory using solid or liquid culture media, which must be sterilized to obtain a pure culture and prevent contamination.
4) The bacterial growth curve consists of four phases: lag, log (exponential), stationary, and death phases. The bacteria acclimate, multiply rapidly, stop growing due to lack of nutrients,
This research aimed to characterize bacteria isolated from soils in Puerto Rico. Two soil samples were collected and various tests were performed on the isolated bacteria, including Gram staining, DNA purification and amplification, and testing for antibiotic production. One isolated bacterium (S15UPRC-RISEAMGP30SP01A1) was identified as a gram-positive cocci resembling Staphylococcus and did not produce antibiotics. The other (S15UPRC-RISERBCR30P01A2) was a gram-positive Bacillus but did not produce antibiotics. Neither bacterium could be fully characterized due to negative PCR results, failing to confirm the hypothesis of positive antibiotic production. Further tests are needed to fully characterize the
The document discusses general principles for diagnosing infectious diseases, including:
1. Physical examination, clinical diagnosis, and epidemiological assessment help identify possible pathogens.
2. Laboratory tests are needed to confirm the causative agent, including microscopic examination, culture-based methods, and immunological or molecular detection techniques.
3. Proper specimen collection, transport, and timing are important for accurate diagnostic results.
The document discusses various methods for diagnosing important bacterial diseases through laboratory examination. Effective diagnosis allows for timely treatment and control measures. Key methods discussed include microscopy, culture techniques, biochemical reactions, serological identification, and molecular diagnosis. Microscopy can identify bacterial morphology and staining properties. Culture techniques isolate bacteria on selective media and examine colony characteristics. Biochemical tests identify metabolic properties. Serology detects bacterial antigens and antibodies. Molecular methods like PCR and sequencing provide sensitive, specific identification and can detect non-culturable bacteria. Together, these diagnostic methods allow clinicians to initiate appropriate treatment and control of bacterial outbreaks.
The document provides information on the diagnosis of important bacterial diseases. It discusses the importance of quick diagnostic results for effective treatment during disease outbreaks. It covers the types and activities of various antimicrobial classes against different bacteria and microorganisms. It also describes the scope of bacterial infections, types of bacteria, why diagnosis is needed, recommended diagnostics for various diseases, steps in diagnosis, prerequisites for laboratory examination including appropriate sample collection and transport, and various microbiological diagnostic techniques like microscopy, culture, and biochemical identification.
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This document provides an overview of wound healing, its functions, stages, mechanisms, factors affecting it, and complications.
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Healing can occur in two ways: Regeneration and Repair
There are 4 phases of wound healing: hemostasis, inflammation, proliferation, and remodeling. This document also describes the mechanism of wound healing. Factors that affect healing include infection, uncontrolled diabetes, poor nutrition, age, anemia, the presence of foreign bodies, etc.
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LAND USE LAND COVER AND NDVI OF MIRZAPUR DISTRICT, UPRAHUL
This Dissertation explores the particular circumstances of Mirzapur, a region located in the
core of India. Mirzapur, with its varied terrains and abundant biodiversity, offers an optimal
environment for investigating the changes in vegetation cover dynamics. Our study utilizes
advanced technologies such as GIS (Geographic Information Systems) and Remote sensing to
analyze the transformations that have taken place over the course of a decade.
The complex relationship between human activities and the environment has been the focus
of extensive research and worry. As the global community grapples with swift urbanization,
population expansion, and economic progress, the effects on natural ecosystems are becoming
more evident. A crucial element of this impact is the alteration of vegetation cover, which plays a
significant role in maintaining the ecological equilibrium of our planet.Land serves as the foundation for all human activities and provides the necessary materials for
these activities. As the most crucial natural resource, its utilization by humans results in different
'Land uses,' which are determined by both human activities and the physical characteristics of the
land.
The utilization of land is impacted by human needs and environmental factors. In countries
like India, rapid population growth and the emphasis on extensive resource exploitation can lead
to significant land degradation, adversely affecting the region's land cover.
Therefore, human intervention has significantly influenced land use patterns over many
centuries, evolving its structure over time and space. In the present era, these changes have
accelerated due to factors such as agriculture and urbanization. Information regarding land use and
cover is essential for various planning and management tasks related to the Earth's surface,
providing crucial environmental data for scientific, resource management, policy purposes, and
diverse human activities.
Accurate understanding of land use and cover is imperative for the development planning
of any area. Consequently, a wide range of professionals, including earth system scientists, land
and water managers, and urban planners, are interested in obtaining data on land use and cover
changes, conversion trends, and other related patterns. The spatial dimensions of land use and
cover support policymakers and scientists in making well-informed decisions, as alterations in
these patterns indicate shifts in economic and social conditions. Monitoring such changes with the
help of Advanced technologies like Remote Sensing and Geographic Information Systems is
crucial for coordinated efforts across different administrative levels. Advanced technologies like
Remote Sensing and Geographic Information Systems
9
Changes in vegetation cover refer to variations in the distribution, composition, and overall
structure of plant communities across different temporal and spatial scales. These changes can
occur natural.
Strategies for Effective Upskilling is a presentation by Chinwendu Peace in a Your Skill Boost Masterclass organisation by the Excellence Foundation for South Sudan on 08th and 09th June 2024 from 1 PM to 3 PM on each day.
This slide is special for master students (MIBS & MIFB) in UUM. Also useful for readers who are interested in the topic of contemporary Islamic banking.
ISO/IEC 27001, ISO/IEC 42001, and GDPR: Best Practices for Implementation and...PECB
Denis is a dynamic and results-driven Chief Information Officer (CIO) with a distinguished career spanning information systems analysis and technical project management. With a proven track record of spearheading the design and delivery of cutting-edge Information Management solutions, he has consistently elevated business operations, streamlined reporting functions, and maximized process efficiency.
Certified as an ISO/IEC 27001: Information Security Management Systems (ISMS) Lead Implementer, Data Protection Officer, and Cyber Risks Analyst, Denis brings a heightened focus on data security, privacy, and cyber resilience to every endeavor.
His expertise extends across a diverse spectrum of reporting, database, and web development applications, underpinned by an exceptional grasp of data storage and virtualization technologies. His proficiency in application testing, database administration, and data cleansing ensures seamless execution of complex projects.
What sets Denis apart is his comprehensive understanding of Business and Systems Analysis technologies, honed through involvement in all phases of the Software Development Lifecycle (SDLC). From meticulous requirements gathering to precise analysis, innovative design, rigorous development, thorough testing, and successful implementation, he has consistently delivered exceptional results.
Throughout his career, he has taken on multifaceted roles, from leading technical project management teams to owning solutions that drive operational excellence. His conscientious and proactive approach is unwavering, whether he is working independently or collaboratively within a team. His ability to connect with colleagues on a personal level underscores his commitment to fostering a harmonious and productive workplace environment.
Date: May 29, 2024
Tags: Information Security, ISO/IEC 27001, ISO/IEC 42001, Artificial Intelligence, GDPR
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it describes the bony anatomy including the femoral head , acetabulum, labrum . also discusses the capsule , ligaments . muscle that act on the hip joint and the range of motion are outlined. factors affecting hip joint stability and weight transmission through the joint are summarized.
2. Methods of the oxygen reduction for anaerobic
bacteria culture:
Kitt-Tarozzi medium
MPB with 1% glucose and pieces of cooked liver
or fat-free meat on bottom for absorption of gases.
The medium should be heated before inoculation
for 10-20 minutes in order to remove the air
oxygen and then cool and inoculated. After
inoculation the medium should be covered with a
layer of sterile vaseline
The material is added into two test tubes, one of
which is heated to 80 ° C for 20 minutes in order
to destroy the non-spore forms. The growth of
bacteria is determined by the turbidity of the
medium and the formation of bubbles of carbon
dioxide on the surface of a layer of liquid vaseline.
Non-spore bacteria grow only in a test tube
without heating, spore bacteria - clostridia - in
both tubes.
3. Methods of the oxygen reduction for anaerobic
bacteria culture:
Gas Pak
4. Methods of the oxygen reduction for anaerobic
bacteria culture:
Biological method
5. Isolation of anaerobic bacteria in pure culture from
clinical specimens for bacteria identification
Day 1
1. Patients’ samples are collected and inoculated into 2-4 tubes with
Kitt-Tarozzi medium (enrichment media). One tube is set unheated,
and the other is heated at 800C for 20 minutes.
Incubate at 370C for 18-24h
Day 2
1. Study of media turbidity and gases formation
- Whithout heating - After 800C heating for 25 min
Differentiation of non-sporing and sporing anaerobes,
anaerobes and aerobes, vegetative cells and
heat resistant spores
6. Isolation of anaerobic bacteria in pure culture from
clinical specimens for bacteria identification
Day 2
2. Smear preparation and staining: (Gram stain,
Special staining for spores demonstration)
spores
Ozheshko stain
vegetative cells
7. Isolation of anaerobic bacteria in pure culture from
clinical specimens for bacteria identification
Day 2
3. Subculture of bacteria from enrichment media for pure culture
isolation may be run by two methods:
• Zeissler’s method (transfer method).
On the firs and second plate the confluent growth may not allow
obtaining well-separated colonies. They will be seen then in the third
plate, where much less bacteria grow.
• Veinberg’s method (sequential dilutions).
The colonies of anaerobes will appear black in this medium and the
separated colonies can be examined and extracted for subculture.
Incubate at 370C for 18-24h
8. Scheme of pathogenic Clostridia pure culture isolation
(Zeisler’s method and Veinberg’s method) and identification
Specimen: ______________________________________________________________
1 stage Kit-Tarozzi liquid medium
After inoculation medium is heated
under 80о
С for 25 min
1. 2.
Gram stain Ozheshko stain
2 stage Zeisler’s method Veinberg’s method
Sugar blood agar
Wilson-Blair agar
3 stage
9. Isolation of anaerobic bacteria in pure culture from
clinical specimens for bacteria identification
Day 3
• Veinberg’s method (sequential dilutions).
The colonies of anaerobes will appear black in this medium and the
separated colonies can be examined and extracted for subculture.
10. Isolation of anaerobic bacteria in pure culture from
clinical specimens for bacteria identification
Day 3 Typical colonies should be examined and smear prepared for
checking of the purity of the colonies.
If the colonies are pure they
may be subcultured
Subculture of the typical pure colonies in Kitt-Tarozzi medium Obtaining of the enough
growth of bacteria for culture
identification
Day 4 Study of Kitt-Tarozzi media turbidity and gases formation If positive – bacterial growth
occurs.
Smear preparation and staining:
Gram stain; Special staining for spores demonstration
The appearance of Gram+
rods with spores indicates the
presence of Clostridia
Subculture of bacteria from enrichment media in differential
(Hiss` media etc.) for pure culture identification.
If the bacterial growth is pure
culture the bacteria may be
subcultured
Day 5 Demonstration of sugarlytic and proteolytic properties,
Study of antigenic structure.
Run of neutralization reaction in lab animals.
Culture identification.
Identification of the type of
toxin for proper
immunotherapy.
11. Isolation of anaerobic bacteria in pure culture from
clinical specimens for bacteria identification
Species Carbohydrates fermentation Spores
location
Oxygen
requirements
Glu Lac Mal
1 Cl. botulinum
AG - AG
subterminal Obligate
anaerobes
2 Cl. tetani
- - -
terminal Obligate
anaerobes
3 Cl. perfringens AG AG AG subterminal microaerofilic
Differential properties of Clostridia spp.
