This document discusses using a systems biology approach to identify external proteins involved in maintaining the stemness of human embryonic stem cells. Microarray and protein interaction data will be analyzed to build an interactome model and identify candidate proteins. In silico experiments and predictions will be used to design further experimental validation, including RNA interference and qPCR experiments using hESCs and differentiated cells, to better understand stemness and support applications in regenerative medicine and drug screening.

1. a Systems Biology approach
identifying external relays involved in stemness
of human embryonic stem cells

2. hESCs
Blastocyst

4. Stemness

5. • Fundamental research
•Regenerative medicine
• Drug screening
The human embryonic stem cells

6. Data analysis
Microarrays

7. Interactome
Data analysis
Model design
Microarrays
Transcriptome
Protein-Protein interaction network
• Node = Protein
• Edge = Physical interaction

8. Enrichments
Interactome
Data analysis
Model design
DRY / In silico
experiments
Microarrays
Transcriptome

9. Enrichments
Interactome
Data analysis
Model design
Predictions/
Hypothesis
DRY / In silico
experiments
Microarrays
Transcriptome
Candidates

10. Enrichments
Interactome
Data analysis
Model design
Predictions/
Hypothesis
DRY / In silico
experiments
Microarrays
Transcriptome
Candidates

11. Enrichments
Interactome
Data analysis
Model design
Predictions/
Hypothesis
DRY / In silico
experiments
Microarrays
Transcriptome
Candidates

12. 0
1
2
3
4
5
hESCs
Differentiated cells
Enrichments
Interactome
Data analysis
Model design
Experimental
design
Predictions/
Hypothesis
DRY / In silico
experiments
Microarrays
Transcriptome
Candidates
qPCR

13. 0
1
2
3
4
5
hESCs
Differentiated cells
Enrichments
Interactome
Data analysis
Model design
Experimental
design
Predictions/
Hypothesis
DRY / In silico
experiments
Microarrays
Transcriptome
Candidates
qPCR

14. Enrichments
Interactome
Data analysis
Model design
WET / In vitro
experiments
Experimental
design
Predictions/
Hypothesis
DRY / In silico
experiments
Microarrays
Transcriptome
Candidates
hESCHEK 293T
RNA interference
qPCR

15. Enrichments
Interactome
Data analysis
Model design
WET / In vitro
experiments
Experimental
design
Predictions/
Hypothesis
DRY / In silico
experiments
Microarrays
Transcriptome
Candidates
RNA interference
qPCR

16. Understanding better the stemness
In order to mobilise the potential of hESCs
The human embryonic stem cells
@VMournet
Alper Uzun, PhD.