Special guest researcher Dr. Lorenzo Rossi of the University of Florida, along with application scientist, Galen George, review real life published studies where root research has been used and will discuss where it's headed in the future.

1. Back to Our Roots:
A look at what's
happening in root
research now and into
the future
CI-600 In-situ Root Imager
CI-602 Narrow Gauge Root Imager
Webinar and Live Q&A
We will begin the webinar at 8:00 AM (PST) Sharp

2. Suzy is the Distributor Manager at
CID Bio-Science. She has been with
CID Bio-Science and CID Bio-Science
for 8 years.
Suzy Truitt
Webinar Moderator

3. Housekeeping
For ALL questions, please use the
Q&A function in Zoom.
Please refrain from using the Chat
function (highlighted in orange)
unless you are experiencing technical
difficulties – such as lack of sound or
video.
Suzy will be posting all relevant links
and any extra information needed in
the Chat.
Chat Box Q&A

4. Galen is an Application Scientist at
CID Bio-Science. He holds a BSc in
Biochemistry and a MSc degree in
Food Science from Michigan State
University. He is an IFT Certified
Food Scientist and has previously
managed an analytical food
laboratory and worked as a food
safety consultant for the food
manufacturing, produce, and
cannabis industries.
Galen George, MS, CFS
Webinar Presenter

5. AGENDA
CID Bio-Science
Live Q&A session with a CID
Bio-Science Application
Scientist
Q&A
Who is using the CI-
600/602? How are current
researchers utilizing the CI-
600/602?
Current Research
Introductions and CI-
600/602 overview, features,
and specifications
Overview Guest Speaker
Dr. Lorenzo Rossi from the
University of Florida

6. Founded in 1989, CID Bio-Science applies more than 30 years of experience in plant
research instrumentation to serve the research community and commercial needs.
We create non-destructive measurement tools that help researchers acquire consistent and high-quality data. Our instruments are known for their ability to produce
instant, accurate results, their durability, portability, and for data transparency. All our product line is engineered, tested, and manufactured under the same roof at our
headquarters in Camas, Washington, USA.
COMPANY OVERVIEW
CID Bio-Science

7. CI-600/602
OVERVIEW
Because root-growth dynamics change seasonally and respond
rapidly to various biotic and abiotic stressors, improved visibility
and accessibility to roots throughout the growing season is key
to improving crop management practices.
Non-destructive root images show a variety of features including
root system architecture, timing of new growth or dormancy, root
length or depth, mycorrhizal root tips, fungal infection, and
parasites or nematode cysts.
Installing clear, plastic tubes throughout the field provides
growers with a way to track changes to root systems in response
to fertilizer application, watering schedule, and root die-back
from disease, parasitic nematode attack, and mycorrhizal
inoculation.

8. CI-600/602
FEATURES
High-resolution images (up to 23.5 million pixels)
Linear scanning with no distortion
100, 300, 600, and 1200 DPI (602 only) scanning
resolutions
360-degree scans (21.59 × 19.56cm)
Live-updating root images
Included tablet computer powers scanner, operates
control software, and saves images
Portable and convenient to transport and insert into
root tubes
Allows observation of root growth and behavior over
multiple growing seasons
CI-600 Diameter = 2.5”
CI-602 Diameter = 1.8” (fits 2” inner diameter tubes)

9. RootSnap!
FEATURES
Multi-touch Interface, optimized for touch-screen
Measurements of root length, area, volume, diameter
& branching angle
Reports average values along with individual root
data
Individual roots can be mapped in under 6 seconds
Integrated image enhancement
Automated "Snap to Root" functionality
Comprehensive image analysis package
Time-series root analysis feature
Intuitive and efficient user interface

10. Track establishment and rooting of
new crops throughout the growing
season
Agriculture
Detect and diagnose plant
pathogens and disorders before
changes are visible above ground
Early Disease Detection
The CI-600/602 can be utilized to
time soil amendment applications
with root flush and investigate
efficacy of fertilizer or other soil
treatments.
Miscellaneous
Observe the development and
function of a plant’s root system
over time
Root Physiology
APPLICATIONS
CI-600/602

11. .
Minirhizotron as an In-Situ Tool for Assessing Sugarcane Root System
Growth and Distribution
1
Augusto Yukitaka Pessinatti Ohashi, Regina Célia de Matos Pires, André Luiz Barros de Oliveira Silva, Leonardo Nazário Silva dos Santos, and Edson Eiji Matsura
Agronomic Institute, Brazil; Goiano Federal Institute, Brazil; University of Campinas, Brazil
“Sugarcane roots grows
relatively fast after tube
installation. Ohashi et al. [5]
observed root growth in the first
30 days after installing tubes in
sugarcane second ratoon,
although the highest growth
rate was observed between 38
and 58 days. Our data regarding
first ratoon shows root rapid
growth during first 30 days
(Figure 3). Thus, it is suggested
to acquire root imager at higher
frequencies right after installing
the tubes, and then image
acquisition can be spaced over
time along crop cycle.”

12. “Monthly monitoring of the root zones of five
‘‘on’’ and ‘‘off’’ trees indicated one major
period of root growth during summer and a
shorter period of root growth during early
autumn (Figs. 1C, 1E, and 2). Vegetative shoot
flush and root growth showed alternating
growth patterns in ‘‘off’’ trees and the first
period of root flush started during early
summer (November) after cessation of the
first vegetative shoot flush in spring (Fig. 1C).
This root flush peaked in midsummer
(December), with ‘‘off’ trees showing a more
intense root flush compared with ‘‘on’’ trees,
in which new root growth was almost
completely absent (Fig. 2). A second period
of root flush started in autumn (end of
March), but during winter (from May
onward), no new root growth was observed
(Figs. 1E and 2). Interestingly, root growth on
the western side of the tree was more
pronounced than that of the eastern side of
the tree, for both ‘‘on’’ and ‘‘off’’ trees (Fig. 2).”
Fruit Load Limits Root Growth, Summer Vegetative Shoot Development, and
Flowering in Alternate-bearing ‘Nadorcott’ Mandarin Trees
2
Ockert P.J. Stander , Graham H. Barry, and Paul J.R. Cronjé
Department of Horticultural Science, Citrus Research International (Pty) Ltd, University of Stellenbosch, Private Bag X01, Matieland 7602, South Africa; XLnT Citrus, Cape Town, South Africa; Department of Horticultural
Science, Citrus Research International (Pty) Ltd, University of Stellenbosch, South Africa

13. “In order to test a
measurement of root growth
inhibition by stress condition,
we measure tomato root
growth under salt and metal
stresses treatment. We used
concentrating hydroponic
culture solution or applying
aluminum chloride into culture
solution for tomato hydroponic
culture. We were able to
detect decreased root growth
without destruction at these
conditions.”
Demonstration for non-destructive root growth
measurement at hydroponic culture by using a root imager
3
Takashi Ikeda, Itsuki Sasaki and Akiyoshi Ota
Meiji University, Japan

14. Dr. Lorenzo Rossi is an Assistant Professor affiliated with the Horticultural
Sciences Department and located at the UF/IFAS Indian River Research and
Education Center (IRREC) in Fort Pierce, FL. His main research area is in plant
root biology. He is a horticulturist with specific expertise related to root
anatomy, dynamics and root system architecture. Dr. Rossi’s research program
focuses on improving root health and growth on HLB-affected citrus, leading
to the development of environmentally sound and effective citrus
management methods. Particularly, his field and greenhouse trials aim to
study the root system architecture of HLB-affected citrus root stocks, as well
as, specialty crops such as peaches and olive trees. Dr. Rossi’s laboratory is
equipped with instruments needed for the study of root functions and
architecture, root traits, root physiology and root imaging.
Prior to Dr. Rossi’s appointment at UF/IFAS, he completed two post doctorate
positions: Texas A&M University and North Carolina State University. Originally
from Italy, he received his Ph.D. Degree in Plant Biology from Italy’s most
prestigious scientific university, Scuola Superiore Sant’Anna in Pisa, Italy.
Dr. Lorenzo Rossi
A S S I S TA N T P R O F E S S O R
H O R T I C U LT U R A L S C I E N C E S D E PA R T M E N T
I N D I A N R I V E R R E S E A R C H A N D E D U C AT I O N C E N T E R
U N I V E R S I T Y O F F L O R I D A

15. Rooting for the future
optimizing root and rhizosphere dynamics
for sustainable grapefruit production systems
Lorenzo Rossi, Ph.D.
April 9th, 2020

16. Lorenzo Rossi
Assistant Professor of Plant Root Biology
Plant Root Biology Laboratory
Dept. of Horticultural Sciences
UF/IFAS Indian River REC
Fort Pierce, FL
772-577-7341
l.rossi@ufl.edu
Howdy!

18. Why study root
traits in the citrus
HLB era?

19. 2015-2016
Citrus: a big deal for
Florida’s agriculture
(STOVER, 2016)

20. Citrus greening poses
threats to Florida groves

21. Asian citrus psyllid
(Diaphorina citri)
Candidatus
Liberibacter asiaticus
What is huanglongbing (HLB; citrus greening)?
Phloem
infection
?

22. HLB and roots
• By now, most citrus growers in Florida are well aware of
the foliar symptoms and damage caused by HLB.
• It is important to remember that only half of the tree is
visible. The root system is equally important for tree
health and fruit production but is difficult to observe.
• Candidatus Liberibacter asiaticus, the bacteria that causes
HLB, lives in and interferes with the function of the
phloem.
• This means that Liberibacter moves to and grows in all
parts of the tree.
• Under most environmental conditions and at most times
of the year, the roots of citrus trees act as sinks.

23. • To identify roots traits that can confer
resistance and/or tolerance
properties to rootstocks
• To correlate root traits to yield, fruit
quality, nutrient uptake efficiency
and environmental stresses resilience
• To help breeding programs in
creating citrus rootstocks that are
HLB resistance/tolerant
• To uncover the genetic, physiological,
and ecological basis of HLB infections

24. Field studies

26. Minirhizotrones
RootSnap! ™
In total: more than 600 mini-rhizotrones across the state of Florida in
collaboration with other Faculty members at UF/IFAS
200 mini-rhizotrones installed on grapefruit roots

27. Future perspectives
in HLB research
• Determination of optimal nutrient concentrations
for citrus under HLB-endemic conditions will
improve root health and increase tree productivity
• Development of new tools and approaches to
characterize citrus root-system structure,
architecture, and function will help uncovering the
genetic, physiological, and ecological basis of HLB
infections.
• Define the extent to which progression of HLB
involves changes in the relationship between the
plant and the soil microbial community will provide
us guidelines for rhizosphere management.

29. Acknowledgement

30. Thank you!
Plant Root Biology Laboratory
l.rossi@ufl.edu
@UFRootBioLab
@UFRoots

31. SUMMARY
Researchers in the academic, private, or government sectors
that require a fast, reliable, and non-destructive way to image
and analyze root systems.
WHO CAN BENEFIT FROM THE CI-600/602?
For any application related inquiries or problems, contact either
Galen George or Eric Munoz-Garcia – CID Bio-Science’s
application scientists!
SUPPORT & COLLABORATION
Uses for the CI-600/602 aren’t limited to just root physiology
studies. The technology can be applied to other fields of
research, such as agriculture, ecology, forestry, and many more!
APPLICATIONS AND USE CASES
The CI-600 and CI-602 provide the means to acquire rapid non-
destructive high-resolution root images in-situ. RootSnap!
Analysis software allows for the user to determine root
measurements by simply tracing the roots on the image.
BUILT FOR ACCURACY, SPEED, AND RELIABILITY

32. Built-in indices for analytes such as chlorophyll, anthocyanins,
carotenoids, and more! PLSR Modeling and FruitMaps
compatibility!
Reflectance / Absorbance / Transmittance
NEW CI-710 Leaf Spectrometer
Instantly and non-destructively assess Kiwi quality and maturity
indices like dry matter and brix.
Dry Matter / Brix
F-751 Kiwi Quality Meter

33. Get Connected
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CID Bio-Science, Inc.

34. DO YOU
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QUESTIONS?
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