The document summarizes a study that examined the mortality effects of transfluthrin diffusers on three types of mosquitoes - pyrethroid-susceptible Aedes aegypti, pyrethroid-susceptible Culex quinquefasciatus, and pyrethroid-resistant Aedes aegypti. The study was conducted over 8 weeks inside and outside US military tents in northern Florida. The results showed that transfluthrin diffusers were highly effective at killing susceptible mosquitoes but not resistant mosquitoes, and the effectiveness varied based on environmental factors like temperature and the location of sentinel cages.

1. Assessing transfluthrin mortality against Aedes aegypti
and Culex quinquefasciatus inside and outside US military
tents in a northern Florida environment
Registration No. 22MP02013 / Paper Code :-MPHE203/Group ‘’C”
1
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Benjamin E. McMillan/2022

2. Contents
 Introduction
 Materials And Methods
 Results
 Discussions
 Conclusion
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3. Introduction
 The study examined the mortality effects of passive resin transfluthrin
diffusers on three types of mosquitoes:
a. Pyrethroid-susceptible Aedes aegypti (colony-reared sentinel strain)
b. Pyrethroid-susceptible Culex quinquefasciatus (colony-reared sentinel strain)
c. Pyrethroid-resistant Aedes aegypti (a separate strain with resistance to
insecticides).
 The transfluthrin diffusers released approximately 5 mg of active ingredient
per 24 hours.
 The study was conducted over an 8-week period.
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4. Introduction
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The study and analyzed the over an 8-week period.
Use
Transfluthrin
Use of
Spatial
Repellents in
Military IVM
Operational
Constraints
in Military
Scenarios
Passive
Control
Techniques
Effectiveness
of Spatial
Repellents
Research
Objectives

5. Materials And Methods
 Several environmental factors were investigated for their impact on mosquito
mortality:
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Effect of
Environmental
Significant
Interactions
Sentinel Cage
Location
Temperature positively correlated with an increase in observed mean
mosquito mortality, suggesting higher temperatures contributed to
greater mortality.
. These interactions showed complex relationships between these
factors and their influence on mosquito mortality.
The mortality measurements varied based on where the
sentinel cages were placed (inside the tent, at the tent
doorway, or outside the tent).

6. Materials And Methods
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Study location and treatment on 29.866178°N,
82.040696°W
A. Inside the
tent, at the
tent B.
doorway, or
C/D. outside
the tent E.
Collections).
1-s2.0-
S2667114X2100

7. Materials And Methods
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Statistical
analysis
ANOVA
Effect of
Temperature:
Mosquito
Strain
Exposure
Time
Data
Representation
Environmental
Data

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Results

9. Results
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10. Discussions
 Transfluthrin diffusers are effective in killing susceptible
Aedes aegypti and Culex quinquefasciatus mosquitoes, but not
pyrethroid-resistant Ae. aegypti mosquitoes.
 The transfluthrin-infused devices' effectiveness was influenced
by environmental factors, which combined to impact mosquito
mortality in the field.
 1-s2.0-S2667114X21000613-mmc1.xlsx (live.com)
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11. Conclusion
 Transfluthrin was highly effective in causing
mortality against the susceptible Ae. aegypti
and Cx. quinquefasciatus mosquitoes under
field conditions.
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12. This Presentation is Prepared by
"Wash Your Hands Regularly, Wear Your Masks Properly & Maintain Safe Distance"
“Rohan Ranjan Waliya”
Class-MPHE /Group : “C”/Pwaliya8@pu.ac.in