Fourier-transform Infrared Analysis and In Vitro Antibacterial Activity of Or...BRNSS Publication Hub
The present research is to assess the Fourier transform infrared (FT-IR) analysis and antibacterial activities of Ormocarpum cochinchinense leaf extract using different solvents dimethyl sulfoxide (DMSO), ethyl acetate, ethanol (EtOH), methanol (MeOH), and chloroform. To investigation of FT-IR analysis and antibacterial activities become used agar well diffusion method. FT-IR vibrational bands confirmed that the fractions of O. cochinchinense had lots of biologically active compounds which include H–Bonded Phenols, alkanes, carboxylic acid, carboxylic acid, flavonoids, polyphenols, catechins, aromatics, and aliphatic amines. Antibacterial activity showed that the strongest activities had been produced by MeOH solvent reaction with all the human pathogens. This research may be concluded that MeOH solvent extract of O. cochinchinense might be a capability for the treatment of antibacterial activities.
Fatty Acid Pattern and Alkaloids of Echium RauwolfiiEditor IJCATR
The GC/MS analysis of hexane extract revealed the presence of palmitic acid as saturated fatty acid (1.05%), versus oleic
acid (2.18%), linoleic acid (1.13%), cis-8,11,14-eicosatrienoic acid (2.12%) as unsaturated fatty acids. On the other hand, CH2Cl2
extract contained palmitic acid methyl ester (3.55%), and methyl isostearate (1.17%) as saturated fatty acids, versus linoleic acid
methyl ester (3.57%) and linolenic acid methyl ester (10.01%) as unsaturated fatty acids. The GC/MS analysis of the alkaloid-rich
fraction indicated the presence of the pyrazolidine alkaloids petranine (2.97%), 7-angeloyl-9-(2-methylbutyryl) retronecine (4.22%), 7-
angeloylretronecine (0.59%) and 9-angeloylretronecine (0.47%).
The butanol extract showed the heights DPPH radical scavenging activity (IC50 = 14.3 μg),. while ethyl acetate extract was very weak
in activity (IC50 = 432.3 μg) and no activity with hexane and methylene chloride extract.
The antimicrobial potentials of E. rauwolfii extracts were examined. The inhibition of the fungi species by ethyl acetate extract exert
was comparable to Amphotericin B. The inhibition zone of the butanol extract against Streptococcus pneumonia was comparable to
Ampicillin, against Pseudomonas aeruginosa was comparable to Gentamicin and Escherichia coli was comparable to Gentamicin.
The cytotoxicity against HePG-2 of ethyl acetate extract and butanol extract were “very strong”, and that of hexane extract and
methylene chloride extract were “moderate”, against MCF-7 of ethyl acetate extract and butanol extract were “strong”, that of
methylene chloride extract was “moderate”, and that of hexane extract was “weak” and against HCT-116 of butanol extract was “very
strong”, of ethyl acetate extract was“strong”, of methylene chloride extract and hexane extract were “moderate”.
Fatty Acid Pattern and Alkaloids of Echium RauwolfiiEditor IJCATR
The GC/MS analysis of hexane extract revealed the presence of palmitic acid as saturated fatty acid (1.05%), versus oleic acid (2.18%), linoleic acid (1.13%), cis-8,11,14-eicosatrienoic acid (2.12%) as unsaturated fatty acids. On the other hand, CH2Cl2 extract contained palmitic acid methyl ester (3.55%), and methyl isostearate (1.17%) as saturated fatty acids, versus linoleic acid methyl ester (3.57%) and linolenic acid methyl ester (10.01%) as unsaturated fatty acids. The GC/MS analysis of the alkaloid-rich fraction indicated the presence of the pyrazolidine alkaloids petranine (2.97%), 7-angeloyl-9-(2-methylbutyryl) retronecine (4.22%), 7-angeloylretronecine (0.59%) and 9-angeloylretronecine (0.47%).
The butanol extract showed the heights DPPH radical scavenging activity (IC50 = 14.3 µg),. while ethyl acetate extract was very weak in activity (IC50 = 432.3 µg) and no activity with hexane and methylene chloride extract.
The antimicrobial potentials of E. rauwolfii extracts were examined. The inhibition of the fungi species by ethyl acetate extract exert was comparable to Amphotericin B. The inhibition zone of the butanol extract against Streptococcus pneumonia was comparable to Ampicillin, against Pseudomonas aeruginosa was comparable to Gentamicin and Escherichia coli was comparable to Gentamicin.
The cytotoxicity against HePG-2 of ethyl acetate extract and butanol extract were “very strong”, and that of hexane extract and methylene chloride extract were “moderate”, against MCF-7 of ethyl acetate extract and butanol extract were “strong”, that of methylene chloride extract was “moderate”, and that of hexane extract was “weak” and against HCT-116 of butanol extract was “very strong”, of ethyl acetate extract was“strong”, of methylene chloride extract and hexane extract were “moderate”.
Monographic Analysis of Catharanthus roseus.pptxvaibhavnamdev5
Catharanthus roseus, commonly known as bright eyes, Cape periwinkle, graveyard plant, Madagascar periwinkle, old maid, pink periwinkle, rose periwinkle, is a perennial species of flowering plant in the family Apocynaceae. It is native and endemic to Madagascar, but grown elsewhere as an ornamental and medicinal plant.
The tropical plant Catharanthus roseus (L) G. Don is one of the most studied plants because it is considered as a medicinal plant. C. roseus synthesize terpene indole alkaloids (TIA) with high medicinal and economical value such as ajmalicine, catharanthine, vindoline, vinblastine and vincristine, the last are considered anti-tumor agents.
This is a study undertaken on nutrient compositions of and processed products development from Son Tra (Docynia indica. The in-depth study of some bioactive substances of son tra fruit and its processed product development was undertaken as part of understanding existing market value chains, and identifying the necessary interventions to improve the performance of son tra.
Fourier-transform Infrared Analysis and In Vitro Antibacterial Activity of Or...BRNSS Publication Hub
The present research is to assess the Fourier transform infrared (FT-IR) analysis and antibacterial activities of Ormocarpum cochinchinense leaf extract using different solvents dimethyl sulfoxide (DMSO), ethyl acetate, ethanol (EtOH), methanol (MeOH), and chloroform. To investigation of FT-IR analysis and antibacterial activities become used agar well diffusion method. FT-IR vibrational bands confirmed that the fractions of O. cochinchinense had lots of biologically active compounds which include H–Bonded Phenols, alkanes, carboxylic acid, carboxylic acid, flavonoids, polyphenols, catechins, aromatics, and aliphatic amines. Antibacterial activity showed that the strongest activities had been produced by MeOH solvent reaction with all the human pathogens. This research may be concluded that MeOH solvent extract of O. cochinchinense might be a capability for the treatment of antibacterial activities.
Fatty Acid Pattern and Alkaloids of Echium RauwolfiiEditor IJCATR
The GC/MS analysis of hexane extract revealed the presence of palmitic acid as saturated fatty acid (1.05%), versus oleic
acid (2.18%), linoleic acid (1.13%), cis-8,11,14-eicosatrienoic acid (2.12%) as unsaturated fatty acids. On the other hand, CH2Cl2
extract contained palmitic acid methyl ester (3.55%), and methyl isostearate (1.17%) as saturated fatty acids, versus linoleic acid
methyl ester (3.57%) and linolenic acid methyl ester (10.01%) as unsaturated fatty acids. The GC/MS analysis of the alkaloid-rich
fraction indicated the presence of the pyrazolidine alkaloids petranine (2.97%), 7-angeloyl-9-(2-methylbutyryl) retronecine (4.22%), 7-
angeloylretronecine (0.59%) and 9-angeloylretronecine (0.47%).
The butanol extract showed the heights DPPH radical scavenging activity (IC50 = 14.3 μg),. while ethyl acetate extract was very weak
in activity (IC50 = 432.3 μg) and no activity with hexane and methylene chloride extract.
The antimicrobial potentials of E. rauwolfii extracts were examined. The inhibition of the fungi species by ethyl acetate extract exert
was comparable to Amphotericin B. The inhibition zone of the butanol extract against Streptococcus pneumonia was comparable to
Ampicillin, against Pseudomonas aeruginosa was comparable to Gentamicin and Escherichia coli was comparable to Gentamicin.
The cytotoxicity against HePG-2 of ethyl acetate extract and butanol extract were “very strong”, and that of hexane extract and
methylene chloride extract were “moderate”, against MCF-7 of ethyl acetate extract and butanol extract were “strong”, that of
methylene chloride extract was “moderate”, and that of hexane extract was “weak” and against HCT-116 of butanol extract was “very
strong”, of ethyl acetate extract was“strong”, of methylene chloride extract and hexane extract were “moderate”.
Fatty Acid Pattern and Alkaloids of Echium RauwolfiiEditor IJCATR
The GC/MS analysis of hexane extract revealed the presence of palmitic acid as saturated fatty acid (1.05%), versus oleic acid (2.18%), linoleic acid (1.13%), cis-8,11,14-eicosatrienoic acid (2.12%) as unsaturated fatty acids. On the other hand, CH2Cl2 extract contained palmitic acid methyl ester (3.55%), and methyl isostearate (1.17%) as saturated fatty acids, versus linoleic acid methyl ester (3.57%) and linolenic acid methyl ester (10.01%) as unsaturated fatty acids. The GC/MS analysis of the alkaloid-rich fraction indicated the presence of the pyrazolidine alkaloids petranine (2.97%), 7-angeloyl-9-(2-methylbutyryl) retronecine (4.22%), 7-angeloylretronecine (0.59%) and 9-angeloylretronecine (0.47%).
The butanol extract showed the heights DPPH radical scavenging activity (IC50 = 14.3 µg),. while ethyl acetate extract was very weak in activity (IC50 = 432.3 µg) and no activity with hexane and methylene chloride extract.
The antimicrobial potentials of E. rauwolfii extracts were examined. The inhibition of the fungi species by ethyl acetate extract exert was comparable to Amphotericin B. The inhibition zone of the butanol extract against Streptococcus pneumonia was comparable to Ampicillin, against Pseudomonas aeruginosa was comparable to Gentamicin and Escherichia coli was comparable to Gentamicin.
The cytotoxicity against HePG-2 of ethyl acetate extract and butanol extract were “very strong”, and that of hexane extract and methylene chloride extract were “moderate”, against MCF-7 of ethyl acetate extract and butanol extract were “strong”, that of methylene chloride extract was “moderate”, and that of hexane extract was “weak” and against HCT-116 of butanol extract was “very strong”, of ethyl acetate extract was“strong”, of methylene chloride extract and hexane extract were “moderate”.
Monographic Analysis of Catharanthus roseus.pptxvaibhavnamdev5
Catharanthus roseus, commonly known as bright eyes, Cape periwinkle, graveyard plant, Madagascar periwinkle, old maid, pink periwinkle, rose periwinkle, is a perennial species of flowering plant in the family Apocynaceae. It is native and endemic to Madagascar, but grown elsewhere as an ornamental and medicinal plant.
The tropical plant Catharanthus roseus (L) G. Don is one of the most studied plants because it is considered as a medicinal plant. C. roseus synthesize terpene indole alkaloids (TIA) with high medicinal and economical value such as ajmalicine, catharanthine, vindoline, vinblastine and vincristine, the last are considered anti-tumor agents.
This is a study undertaken on nutrient compositions of and processed products development from Son Tra (Docynia indica. The in-depth study of some bioactive substances of son tra fruit and its processed product development was undertaken as part of understanding existing market value chains, and identifying the necessary interventions to improve the performance of son tra.
General principles involved in management of poisoning- by rxvichu!!RxVichuZ
Hellow friends!!! I am back....with my 13th ppt!!
This ppt is regarding TOXICOLOGY,which happens to be my 1st....and i am happy to release the same on INDEPENDENCE DAY!!
Wishing a very happy and blissful Independence Day to all....i release my toxicology ppt regarding GENERAL PRINCIPLES IN POISONING MANAGEMENT.....
Since its my 1st attempt in Toxicology, i would love to hear ur reviews, and comments....so that i can improve in upcoming editions......
Keep reading...thanks for ur support!!!
With love and regards,
Vishnu.R.Nair (rxvichu-alwz4uh!!)
:) :)
Total Antioxidant Capacity of Labdane and Pimarane Diterpenoids of Juniperus ...Editor IJCATR
Although Juniperus phoenicea L is a widely distributed wild tree in the south of Saudi Arabia, but its phytochemical and physiological evaluation is still poor. The chromatographic separation of the CH2Cl2/MeOH, 1:1 extract of J, phoenicea L. fruits gave β-sitosterol, stigmasterol, four labdane and two pimarane diterpenoids, including new labdane diterpenoid. Additionally, the volatile compounds of the main petroleum ether extract, as well as the steam-volatile constituents were identified by GC/MS. The separated compounds were identified by spectral tools. The new diterpenoid was identified as 5,9,10-triepicupressic acid.
A previous false identification of sandracopimaric acid, from the fruits of Juniperus phoenicea L. grown in Egypt, was detected and declared to be revised.
Total antioxidant capacity of extracts was estimated using total antioxidant capacity kit of Biodiagnostic, based on Koracevic et al., for biological fluids, with slight modification to suit extracts. Ascorbic acid was used as a reference antioxidant compound. Reasonable results were obtained by applying the modified method on petroleum ether extract, methylene chloride extract and ethyl acetate extract of Juniperus phoenicea L.
Total Antioxidant Capacity of Labdane and Pimarane Diterpenoids of Juniperus ...Editor IJCATR
Although Juniperus phoenicea L is a widely distributed wild tree in the south of Saudi Arabia, but its
phytochemical and physiological evaluation is still poor. The chromatographic separation of the CH2Cl2/MeOH,
1:1 extract of J, phoenicea L. fruits gave β-sitosterol, stigmasterol, four labdane and two pimarane diterpenoids,
including new labdane diterpenoid. Additionally, the volatile compounds of the main petroleum ether extract, as
well as the steam-volatile constituents were identified by GC/MS. The separated compounds were identified by
spectral tools. The new diterpenoid was identified as 5,9,10 -triepicupressic acid.
A previous false identification of sandracopimaric acid, from the fruits of Juniperus phoenicea L. grown in Egypt,
was detected and declared to be revised.
Total antioxidant capacity of extracts was estimated using total antioxidant capacity kit of Biodiagnostic, based on
Koracevic et al., for biological fluids, with slight modification to suit extracts. Ascorbic acid was used as a
reference antioxidant compound. Reasonable results were obtained by applying the modified method on petroleum
ether extract, methylene chloride extract and ethyl acetate extract of Juniperus phoenicea L.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
General principles involved in management of poisoning- by rxvichu!!RxVichuZ
Hellow friends!!! I am back....with my 13th ppt!!
This ppt is regarding TOXICOLOGY,which happens to be my 1st....and i am happy to release the same on INDEPENDENCE DAY!!
Wishing a very happy and blissful Independence Day to all....i release my toxicology ppt regarding GENERAL PRINCIPLES IN POISONING MANAGEMENT.....
Since its my 1st attempt in Toxicology, i would love to hear ur reviews, and comments....so that i can improve in upcoming editions......
Keep reading...thanks for ur support!!!
With love and regards,
Vishnu.R.Nair (rxvichu-alwz4uh!!)
:) :)
Total Antioxidant Capacity of Labdane and Pimarane Diterpenoids of Juniperus ...Editor IJCATR
Although Juniperus phoenicea L is a widely distributed wild tree in the south of Saudi Arabia, but its phytochemical and physiological evaluation is still poor. The chromatographic separation of the CH2Cl2/MeOH, 1:1 extract of J, phoenicea L. fruits gave β-sitosterol, stigmasterol, four labdane and two pimarane diterpenoids, including new labdane diterpenoid. Additionally, the volatile compounds of the main petroleum ether extract, as well as the steam-volatile constituents were identified by GC/MS. The separated compounds were identified by spectral tools. The new diterpenoid was identified as 5,9,10-triepicupressic acid.
A previous false identification of sandracopimaric acid, from the fruits of Juniperus phoenicea L. grown in Egypt, was detected and declared to be revised.
Total antioxidant capacity of extracts was estimated using total antioxidant capacity kit of Biodiagnostic, based on Koracevic et al., for biological fluids, with slight modification to suit extracts. Ascorbic acid was used as a reference antioxidant compound. Reasonable results were obtained by applying the modified method on petroleum ether extract, methylene chloride extract and ethyl acetate extract of Juniperus phoenicea L.
Total Antioxidant Capacity of Labdane and Pimarane Diterpenoids of Juniperus ...Editor IJCATR
Although Juniperus phoenicea L is a widely distributed wild tree in the south of Saudi Arabia, but its
phytochemical and physiological evaluation is still poor. The chromatographic separation of the CH2Cl2/MeOH,
1:1 extract of J, phoenicea L. fruits gave β-sitosterol, stigmasterol, four labdane and two pimarane diterpenoids,
including new labdane diterpenoid. Additionally, the volatile compounds of the main petroleum ether extract, as
well as the steam-volatile constituents were identified by GC/MS. The separated compounds were identified by
spectral tools. The new diterpenoid was identified as 5,9,10 -triepicupressic acid.
A previous false identification of sandracopimaric acid, from the fruits of Juniperus phoenicea L. grown in Egypt,
was detected and declared to be revised.
Total antioxidant capacity of extracts was estimated using total antioxidant capacity kit of Biodiagnostic, based on
Koracevic et al., for biological fluids, with slight modification to suit extracts. Ascorbic acid was used as a
reference antioxidant compound. Reasonable results were obtained by applying the modified method on petroleum
ether extract, methylene chloride extract and ethyl acetate extract of Juniperus phoenicea L.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
2. ARTEMISININ
SYNONYM: SWEET WORMWOOD, SWEET ANNIE, ANNUAL
WORMWOOD,SWEET SAGEWORT
BIOLOGICAL SOURCE: IT IS A SEMISYNTHETIC
DERIVATIVE OF SESQUITERPENE LACTONE AND
SITUATED IN GLANDULAR TRICHOME OF LEAVE AND
EXTRACTED FROM THE PLANT OF ARTEMISIA ANNUA
(SWEET WORMWOOD).
FAMILY:ASTERACEAE/COMPOSITAE
IT IS MAINLY USED IN CHINESE SYSTEM
OF MEDICINE
GEOGRAPHICAL SOURCE: IT IS
MAINLY DISTRIBUTED IN MILD
LATITUDE OF FOUR CONTINENTS:
1. WESTERN AND CENTRAL
EUROPE
2. SOUTHEASTERN ASIA
3. SOUTHEASTERN NORTH
AMERICA
3. 4. SOUTHEASTERN SOUTH AMERICA
BUT IN INDIA IT IS MAINLY
CULTIVATED IN LUCKNOW AND
SRINAGAR WHICH IS EXTRACTED
BY PETROLEUM ETHER.
CHEMISTRY OF ARTEMISININ:
1. TETRACYCLIC STRUCTURE
WITH TRIOXANE RING AND A
LACTONE RING
2. TRIOXANE RING CONTAIN A
PEROXIDE BRIDGE WHICH IS
ACTIVE MOIETY OF MOLECULE.
ACTIVE CONSTITUENT OF
ARTEMISININ:
ARTEMISININ,DIHYDROARTEMISI
NIN, ARTEMISIN
4. MECHANISM OF ACTION OF
ARTEMISININ:
1. ARTEMISININS ARE PRODRUGS OF THE
BIOLOGICALLY ACTIVE
METABOLITE DIHYDROARTEMISININ
WHICH IS ACTIVE DURING
THE STAGE WHEN THE PARASITE IS
LOCATED INSIDE RBC.
2. SEVERAL LINE OF EVIDENCE INDICATE
THAT ARTEMISININS
EXERT THEIR ANTIMALARIAL ACTION BY
RADICAL
FORMATION THAT
DEPEND ON THEIR ENDOPEROXIDE BRIDGE.
3. WHEN THE PARASITE THAT CAUSES
MALARIA INFECT A RBC, IT
CONSUMES HEMOGLOBIN WITHIN ITS
DIGESTIVE VACUOLE, A
PROCESS THAT GENERATES OXIDATIVE
STRESS.
5. INDUSTRIAL PREPARATION OF ARTEMISININ:
TAKE A DRIED POWDER SAMPLE OF ARTEMISIA ANNUA
MIXED WITH METHANOL FOR 1 HOUR IN SOXHLET APPARATUS
EVAPORATED THE SOLUTION UNDER VACUUM UPTO 100 ML
AND MIXED WITH OR PARTITIONED WITH n-hexane
AFTER MIXING WITH n-hexane COLLECTED THE METHANOL LAYER AND
THAN ADD WATER
AGAIN PARTITIONED WITH ETHYL ACETATE
AFTER THIS 2 LAYER ARE SEPERATED 1. SEMIPOLAR LAYER (ETHYL
ACETATE LAYER) AND 2. POLAR LAYER(METHANOL WATER LAYER)
ETHYL ACETATE LAYER EVAPORATE TO GET CRUDE ARTEMISININ
6. CHEMICAL TEST
1. SAMPLE+fecl3+naI DEEP VIOLET COLOUR
2. SAMPLE+TRIPHENYLPHOSPHINE TRIPHENYLPHOSPHINE
OXIDE(INDICATE THE PRESENCE OF OR EXISTENCE OF AN
OXIDATIVE GROUP)
ESTIMATION AND IDENTIFICATION OF ARTEMISININ:
1. TLC METHOD:
a. PLATE: PRECOATED SILICA GEL 60F254
b. MOBILE PHASE: hexane(n):diethyl ether(6:4v/v)
c. SPRAYING REAGENT: ACETIC ACID : SULPHURIC ACID
:ANISALDEHYDE(100:2:1)
d. Rf: 0.20 ( PINK SPOT)
SAMPLE PREPARATION: 1mg IS EXTRACTED IS DISSOLVED IN 50 ML OF
MOBILE PHASE AND APPLIED IT ON PLATE.
7. STANDARD PREPARATION: 1mg OF STANDARD ARTEMISININ IS
DISSOLVED IN 100ml OF MOBILE PHASE AND APPLIED ON PLATE.
2. HPLC METHOD:
A. EQUIPMENT NAME: WATER C501
B. COLUMN: WATER C18
C. MOBILE PHASE: ACETONITRILE:WATER(85:15V/V)
D. FLOW RATE: 1.0 ml/min
E. DETECTION WAVELENGTH: 216nm-260nm
F. INJECTION VALUE: 20 µl
G. PUMP MODE: ISOCRATIC
H. RUN TIME: 4.68min
SAMPLE PREPARATION: 10mg ARTEMISININ DISSOLVED IN
MOBILE PHASE TO PRODUCE 10 ml
STANDARD PREPARATION: 1. 5g POWDER ARTEMISIA AND MIXED
WITH nhexane FOR 6hr AT 60+-2. AND AMBERED COLOURED FLASK
8. 2.COOLED AT ROOM TEMPERATURE AND FILTER THROUGH WHATMAN
NO 41 AND COLLECTED IN 250 ML AMBER COLOURED VOLUMETRIC
FLASK
3. MARC AGAIN REFLUX WITH 100 ml 0F nhexane FOR 1 HR
4. CONTENT WAS FILTERED AND EVAPORATED TO DRYNESS AT WATER
BATH AT 70 DEGREE CELSIUS
5. RESIDUE DISSOLVED AND DILUTED WITH MOBILE PHASE TO GET
PRODUCT
USE:
1. IT IS USED IN ANTIMALARIAL
2. IT IS USED IN GASTRIC INFECTION
3. IT IS USED IN TREATMENT OF SCHIZOPHRENIA
4. IT IS USED IN COLON AND HUMAN LEUKEMIA
5. IT IS USED IN SEVERAL BLOOD PARASITIC PROTOZOANS DISEASE