This Presentation consists of different applications of GC-MS

1. Presented By – Amit Kumar
Dept.: (M.S. Pharm.) 1st Year
Pharmaceutical Analysis
Reg. No.: 23MSPA013

2. TABLE OF CONTENT
 INTRODUCTION
 WORKING
 APPLICATIONS OF GC-MS
 CONCLUSION
TABLE OF CONTENT

3. Introduction to GC-MS
GC
Separates the
mixture of
chemicals
MS
Provides
Identification &
Structural
information of
each chemicals
based on their
m/z ratio.
 It is actually two techniques that are combined to form a single method of analyzing mixtures of
chemicals.
 GC-MS is used both for the qualitative identification and for the quantitative measurement of individual
components in complex mixtures
 MS is used as a detector.
 Data from a GC-MS is three-dimensional, providing mass spectra that can be used for identity
confirmation or to identify unknown compounds.
 Considered as Gold standard in scientific analysis.

4. (1) carrier gas
(2) autosampler
(3) inlet
(4) analytical column
(5) Interface
(6) Vacuum
(7) ion source
(8) mass analyzer
(9) ion detector and
(10) PC
Pic Credit: Anthias Consulting

5. APPLICATIONS
OF GC-MS

6. DETECTION OF MICROPLASTICS
Pyr-GC/MS is a destructive technique.
 Fast and reproducible Pyr-GC/MS method for
the analysis of the 12 most common plastic
polymers .
1. polystyrene (PS)
2. polyethylene (PE)
3. polypropylene (PP),
4. polyvinylchloride (PVC),
5. polyethylene terephthalate (PET),
6. polycarbonate (PC),
7. polyurethane (PUR),
8. Nylon 6 (N-6),
9. Nylon 66 (N-66),
10. polymethyl methacrylate (PMMA),
11. styrene-butadiene copolymer (SBR)
12. acrylonitrile butadiene styrene copolymer (SBR)
plastic size <5 mm - environmental threat.
 Pyrolyzates and polymers libraries used for fast
identification and quantification

7. FORENSIC APPLICATIONS
• Investigation of fire scenes
• Detection and classification of ignitable liquids
• This tool may help increase the efficiency and objectivity of the forensic laboratory.

8. GEOCHEMICAL RESEARCH
Source: https://rb.gy/o229r3
Rock samples were collected
net zero carbon emission.

9. ASTROCHEMISTRY
• Instrument to a mass of less than 15 kg
• 1’x1’x1’ Size
• Operate robotically
• Survive the rigors of both the journey and the
Martian atmosphere
• Organic analysis of the soil on Mars.

10. DETECTION OF CARCINOMAS
Source: https://rb.gy/yf2ls2

11. CHEMICAL WARFARE
• Accurate detection and identification of dangerous chemicals, including
chemical warfare agent.
• Expanded market demand for small, rapid GC–MS systems (Field Portable)
• E.g: O-ethyl-S-2-diisopropylaminoethyl methylphosphonothiolate (VX)
• Detector : transmission quadrapole and ion trap cyclotron
http://go.aussiebum.com/tCC8Q6

12. DIAGNOSIS OF INFLAMMATORY DISASES
• Potential source for biomarkers.
• Breath components in patients with inflammatory diseases-enabling diagnosis and
treatment assessment

13. PHARMACEUTICALAPPLICATIONS
• Flurbiprofen is a non-steroidal anti-inflammatory agent, one of the propionic
acid group, which has significant anti-inflammatory, analgesic and antipyretic
properties.
• It contains a fluorine atom.

14. ENVIRONMENTAL MONITORING

15. BIOLOGICALANALYSIS
• Bioanalysis of body fluids to detect narcotics, barbiturates, alcohols, and drugs
• Dozens of congenital metabolic diseases called as inborn error of metabolism are now
detectable[11]

16. FOOD AND FRAGRANCE ANALYSIS
https://www.prescouter.com/2019/05/fla
vor-discovery-big-data-ai/

17. ANALYSIS OF PHYTOCONSTITUENTS
https://upload.wikimedia.org/wikipedia/commons/
1/1b/Amomum_nilgiricum_rhizome.jpg
The GC-MS analysis revealed the presence of twenty-three bioactive
compounds which include 9,12,15-octadecatrien-1-ol, n-Hexadecanoic
acid, octadecatrienol acid, methyl palmitate, and phytol.

18. CONCLUSION
GC-MS is an advanced technique that cannot be compared with other modern analytical
equipment but can be complemented by mass spectrophotometer to achieve GC-MS/MS. It has
broad range of applications that caters to academic research, quality control aswell as industrial
applications. Its concise, efficient, automated system gives fast, reproducible and effective results
that serve a key role in advancement of Science and Technology. This versatile analytical
technique could be explored for better prospects in future.[11]

19. REFERENCES
1. https://assets.thermofisher.com/TFS-Assets/CMD/Application-2.Notes/an-65632-gc-ms-
volatile-organic-compounds-drinking-water-an65632-en.pdf
3.https://www.sciencedirect.com/science/article/abs/pii/S1387380610000898?via%3Dihub
4.https://web.archive.org/web/20060829035244/http://appel.nasa.gov/items/Viking_GCMS_ca
se_07%2025%2006.pdf
5.https://analyticalscience.wiley.com/content/article-do/geochemical-gc-ms-guides-oil-shale-
exploration
6.https://pubmed.ncbi.nlm.nih.gov/26333204/
7.https://www.sciencedirect.com/science/article/pii/S2215016123001437
8.https://pubmed.ncbi.nlm.nih.gov/36757384/
9.https://www.sciencedirect.com/science/article/abs/pii/S2468170922000224
10.https://fjps.springeropen.com/articles/10.1186/s43094-021-00208-4
11.https://www.omicsonline.org/open-access/gc-ms-technique-and-its-analytical-applications-
in-science-and-technology-2155-9872.1000222.php?aid=33334
12.https://www.sciencedirect.com/science/article/abs/pii/S0003269723001203#:~:text=Exhaled
%20gas%20can%20be%20analyzed,end%20metabolites%20of%20systemic%20metabolism