Application and evolution techniques of crop response to bio fertilizers & Methods of Inoculation

2. BIO FERTILIZERS
 A biofertilizer is a substance which contains
living micro organism which, when applied to
seeds, plant surfaces, or soil, colonize the
rhizosphere or the interior of the plant and
promotes growth by increasing the supply or
availability of primary nutrients to the host
plant.

3.  Biofertilizer are low cost renewable sources of plant
nutrients.
 Improve soil fertility and crop productivity.
 They are ideal input for starting organic farming.
Maintenance of soil health.
Minimize environmental pollution.
Cut down the use of chemical fertilizer.

4. Media used for mass culture as follows:
 Rhizobium : YEMA ( Yeast extract mannitol Agar)
 Azospirillum : Dobereiners mallic acid broth with
Sodium chloride.
 Azatobactor : Waksmanna No .77 broth.
 Phosbacteria : Pikovaskys broth.
 Pseudomonas : Kings B broth.
 Trichoderma : PDB (Potato Dextrose Broth)

5. Bio – Fertilizer application methods
1. Seed treatment
2. Root dipping
3. Set treatment
4. Soil applications

6. SEED TREATMENT
 Seed treatment is a most common method
adopted for all types of inoculants.
 The seeds treatment is effective and economic.
 The seeds are treated with biofertilizer are kept in
shed for 30 minutes and then seed become ready
for sowing.
 20 gram of biofertilizer is used for 1 kg of seeds.
 50 ml of liqiud biofertilizer is used for 1 kg of
seeds.

8. SET TREATMENT
 Set treatment is used for the crops which
is multiplies by vegetative body of the
crops.
 Eg; Sugarcane, cut pieces of Potato and
base of banana suckers.
 Prepare the culture suspension by 1 kg of
bio fertilizers with 40-50 L of water.

10. SEEDLING DIPPING
 The seedlings are uprooted from nursery and
cleaned their roots in water dipped in solution
of bio fertilizer.
 And kept in atleast 20 minutes and transplant
immediately.
 For root dipping : Dissolve the 1 packet of
biofertilizer with 20 liters of water (200 – 300
plants).
 One packet in 2 liters is suffficient to treat
200- 3oo sets under cutting method.

12. SOIL APPLICATION
 The mixture of bio fertilizer + compost +
soil applied on land before sowing of seed
or transplanting of the main field.
 The mixture of bio fertilizer and cattle
manure/soil sprinkled with water is then
broadcast into the soil at the time of
sowing or at the time of irrigation in
standing crop.