Antibody diversity presentation is created by creative biolabs. In the slideshare, we will detailed the mechanism of antibody divesity-gene rearrangement and antibody sequencing service. Principle of antibody diversity is completely understood. There is very close relationship in amino acide sequence and antibody function. As we know, there is huge diverse function between two antibodies which have almost same amino acid even if one amino acide is different. If you have any quesion, welcome to cantact us at info@creative-biolabs.com.

1. Antibody Diversity-Gene
Rearrangement
www.creative-biolabs.com

2. • Variable Domains
• Antigen-binding
• Activity
• Constant Domains
• Functional
• Activities
Antibody Structure and Function

3. What is Antibody Diversity ? Source of Antibody Diversity
• Millions of antigens
• Produce specific antibody against all antigens
• Diversification
•One gene for each protein.
•There are around 40000 genes in our genome.
But our immune system apparently produce antibody in the order of 1010
• Antibody Gene Rearrangement
• Junctional Diversity
• Antibody Isotype Switching
Problem ?
Antibody Diversity-Gene Rearrangement
Source of antibody
diversity
• Somatic Hypermutation
• Affinity Maturation

4. Hematopoietic stem cell
Lymphoid cell
Pro-B cell
Pre-B cell
Partial heavy-chain gene rearrangment
Complete heavy-chain gene rearrangment
Immature B cell
Light-chain gene rearrangment
Mature B cell
Change in RNA processing
CELL Ig EXPRESSED
None
None
None
μ Heavy chain
+surrogate light chain
mIgM
mIgM + mIgD
B Lymphocyte Development (1)
Bone marrow

5. B Lymphocyte Development （ 2 ）
Mature B cell
Differentiation
IgM-secreting plasma cells
Class switching
Memory B
cells of various
isotypes
Antigen stimulation
IgM
Plasma cells
secreting various
isotype
IgG IgA IgE
mIgM + mIgD
Peripheral lymphoid organs
Activated B cell

6. Organization of lg Genes

7. Somatic recombination
Polypeptide chain
Transcription
Somatic recombination
Germline DNA
D-J rearranged
DNA joined
Splicing
Translation
DNARNAProtein
Light chain Heavy chain
Antibody Gene Rearrangement

8. Heavy Chain Locus and VDJ Rearrangement

9. Kappa Light Chain Rearrangement

10. Recombination Signal Sequences (RSSs)
V(D)J recombinase = RAG1 and RAG2
Looping out of DNA
during rearrangement
Mechanism of Variable-Region DNA Rearrangements
• Between V, D, and J segments
• Signal for recombination
• 2 kinds
• 12 base pairs (bp) – 1 turn of DNA
• 23 bp – 2 turns of DNA
• 12 can only join to 23 and vice versa

11. • Monoclonal antibodies
A New World of Possibilities
• Engineering mice with human
immune systems
• Generating chimeric and
hybrid antibodies for clinical
use
• Antibody library: construct
more effective Ab for target

12. Antibody Sequencing Service by Creative Biolabs
• 100% accuracy
• For different
isotypes
• Within 10 days • For Various types
of antibody

13. CONTACT US
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-871-5806 Fax: 1-631-207-8356
Email: info@creative-biolabs.com
Web: www.creative-biolabs.com