Adhatoda vasica and gingiber officinale is medicinal properties are using respiratory tract infection causing disease. " Antibacterial activity of adhadosa vasica and gingiber offivinale against respiratory infections causing bacteria

1. Antibacterial activity of Adhatoda Vasica and
Zingiber officinale against Respiratory
Infection Causing Bacteria
M. Kokila
Ph. D Research Scholar
Department of Microbiology
Annamlai University
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2. Introduction:
Respiratory tract infections are refers to infectious disease
involved in respiratory tract
The respiratory tract infectious microorganism such as
Bacteria and Virus
Respiratory tract infection are classified into two types
1)Lower respiratory tract infection (Pneumonia,
Bronchitis)
2) Upper respiratory tract infection (influenza and
common cold)
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3. LRTI Symptoms:
Shortness of breath weakness,
High fever,
Coughing and Fatigue,
URTI Symptoms:
Cough,
Sore throat,
Runny nose,
Nasal congestion,
Headache,
Low grade fever,
Facial pressure and sneezing 3

4. Objective of the study
•To collect the respiratory tract infection causing bacterial species.
•To collect the Adhatoda vasica and Zingiber officinale leaf.
•To study the antimicrobial activity against the respiratory tract
infection causing bacteria.
Materials and Methods:
The leaf of A. vasica and Zingiber officinale was used in
this experiment were collected from market in Chidambaram.
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5. Test Microorganisms:
• The pathogens used this experiment were obtained from
microbiology laboratory, Department of Microbiology, Annamalai
University, Annamalai nagar.
• Bacterial strains Collected were Staphylococcus aureus,
Pseudomonas aeruginosa and Klebsiella pneumonia.
• The bacterial strains were maintained by transferring them
weekly in nutrient broth.
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6. •The plant leaves was washed with 70% alcohol and then rinsed with sterilized
distilled water.
• Collected leaves were dried in shade at room temperature for 20 days. The
thoroughly air dried leaves milled to powder weighed and stored in bottles.
• 2g of powdered materials mixed with 20ml of sterile distilled water and kept on
rotary shaker for 12 hours at 30ºC. There after it was filtered with the help of
Whatsman no: 1 filter paper.
• The filtrate then centrifuged at 2000 rpm for 10 min then the supernatant was
collected and stored at 4ºC for further use.
Preparation of Adhathoda vasica leaf extract
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7. •The ginger were washed with clean sterile distilled water and allowed to air dry
for one hour.
• 200g fresh ginger were crushed and dried in the sun for 7 days. The dried
rhizome was then crushed in a mortar and pestle to form a fine powder pestle.
• 2g of powdered materials mixed with 20ml of sterile distilled water and kept on
rotary shaker for 12 hours at 30ºC. There after it was filtered with the help of
Whatsman no: 1 filter paper.
• The filtrate then centrifuged at 2000 rpm for 10 min then the supernatant was
collected and stored at 4ºC for further use
Preparation of Ginger extract
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8. Antibacterial Activity
Disc diffusion method:
• In the paper disc diffusion test, sterile paper disc were soaked in
the A. Vasica and ginger extract for 2 hrs.
•The best bacterial strains of 0.2ml were spread on the surface of
Muller Hinton agar plates by using “L” rod and the disc was
placed at the center place of the plate aseptically.
• They were incubated at 37ºC for 24 hrs for the test bacteria.
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9. Results:
The result of the present study showed that the extract of
Adhatoda vasica and ginger (Zingiber officinale) indicated the
presence of potent antibacterial properties against the tested
pathogens such as Staphylococcus aureus, Pseudomonas aeruginosa
and Klebsiella pneumonia. The extract are against respiratory tract
causing bacteria’s
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10. Conclusion:
The present study was undertaken with the main objectives
to study the antibacterial activity of aqueous extract of Adhatoda
vasica and (Ginger) Zingiber Officinale against human respiratory
tract infection causing pathogens such as Staphylococcus aureus,
Pseudomonas aeruginosa. The Adhatoda vasica leaf extract and
Zingiber Officinale was found effective against Staphylococcus
aureus and Klebsiella pneumonia and Pseudomonas aeruginosa
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