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Ammonia Oxidising Archaea
George Foot, Ke Meng & Dan Wilson

Tweetable abstract: Archaea are now recognised as a dominant organism in the
oxidation of ammonia #AOA #HR926EnviroMicrob @GeorgeKeDan

1. Introduction
A fundamental component of the
nitrogen cycle is nitrification (fig. 1).
This involves the biological oxidation of
ammonia to nitrate. Until recently it had
been assumed that ammonia oxidation
was carried out exclusively by ammonia
oxidising bacteria (AOB) [1, 2].
However, subsequent research revealed
that ammonia oxidizing archaea (AOA)
could be more significant in some
environments [fig. 2; 1, 3]

2. The Thaumarchaeota
In 2008 Brochier-Armanet et al.
[4] suggested a third archaeal
phylum characterised by the
ability to oxidise ammonia [5].
These archaea are ubiquitous [6]
and may therefore represent a
significant component of global
ammonia oxidation.

3. Biochemistry

4. Atmospheric chemistry

The biochemistry of archaeal ammonia
oxidation is unique and remains
unresolved. AOA share genes only
distantly related to those encoding the
ammonia monooxygenase (AMO) of AOB.
Currently there is no evidence that the
product of ammonia oxidation by AOA is
hydroxylamine and in fact it has been
suggested that nitroxyl could instead be
the product [7]. Nitroxyl may then be
oxidised to nitrite via a nitroxyl
oxidoreductase [8]. Furthermore Nitric
oxide may also be important in the
archaeal ammonia oxidation process.

AOA generate large amount of greenhouse
gases such as methane and nitrous oxide [5].
Furthermore Nitrosopumilus maritimus has
been demonstrated to be a biological source
of methylphosphonate. This could explain
part of the high concentration of methane in
surface oceans [9].

5. Future research

Rapid development of genome sequencing will
allow more efficient comparative studies
permitting for a greater understanding of the
evolutionary origins of AOA.
Furthermore, future research should focus on
furthering our understanding on AOA
biochemistry.

Figure captions
Figure 1: Schematic of the global N cycle [1]
Figure 2: Archaeal amoA genes compared to
bacterial amoA genes in a range of topsoils and
deeper soil layers[10]

Figure 1: Nitrogen cycle [ref. 1]

Figure 2: AOA & AOB abundance [ref. 10]
References:
[1] Schleper, C. (2008). Metabolism of the deep. Nature, 456, 712-714.
[2] Zhang, L. M., Offre, P. R., He, J. Z., Verhamme, D. T., Nicol, G. W., & Prosser, J. I. (2010). Autotrophic ammonia
oxidation by soil thaumarchaea. Proceedings of the National Academy of Sciences, 107, 17240-17245.
[3] Könneke, M., Bernhard, A.E., José, R., Walker, C.B., Waterbury, J.B., & Stahl, D.A. (2005). Isolation of an autotrophic
ammonia-oxidizing marine archaeon. Nature, 437, 543-546.
[4] Brochier-Armanet C, Boussau B, Gribaldo S, & Forterre P. (2008). Mesophilic Crenarchaeota: proposal for a third
archaeal phylum, the Thaumarchaeota. Nat. Rev. Microbiol., 6, 245–52
[5] Stahl, D.A., & de la Torre, J.R. (2012). Physiology and diversity of ammonia-oxidizing archaea. Annual review of
microbiology, 66, 83-101.
[6] Offre, P., Spang, A., & Schleper, C. (2013). Archaea in biogeochemical cycles. Annu. Rev. Microbiol, 67, 437–57.
[7] Walker CB ,de la Torre JR ,Klotz MG, Urakawa H,Pinel N,et al.(2010). Nitrosopumilus maritimus genome reveals
unique mechanisms for nitrification and autotrophy in globally distributed marine crenarchaea. Proc. Natl. Acad. Sci.,
107, 8818–23
[8] Hatzenpichler, R. (2012). Diversity, Physiology, and Niche Differentiation of Ammonia- Oxidizing Archaea. Applied
and Environmental Microbiology. 78,21, 7501–7510.
[9] Reeburgh W.S. (2007). Oceanic methane biogeochemistry. Chem. Rev., 107,486–513.
[10] Leininger S, Urich T, Schloter M, Schwark L, Qi J, et al. (2006). Archaea predominate among ammonia-oxidizing
prokaryotes in soils. Nature, 442, 806–9.

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Ammonia Oxidising Archaea poster

  • 1. Ammonia Oxidising Archaea George Foot, Ke Meng & Dan Wilson Tweetable abstract: Archaea are now recognised as a dominant organism in the oxidation of ammonia #AOA #HR926EnviroMicrob @GeorgeKeDan 1. Introduction A fundamental component of the nitrogen cycle is nitrification (fig. 1). This involves the biological oxidation of ammonia to nitrate. Until recently it had been assumed that ammonia oxidation was carried out exclusively by ammonia oxidising bacteria (AOB) [1, 2]. However, subsequent research revealed that ammonia oxidizing archaea (AOA) could be more significant in some environments [fig. 2; 1, 3] 2. The Thaumarchaeota In 2008 Brochier-Armanet et al. [4] suggested a third archaeal phylum characterised by the ability to oxidise ammonia [5]. These archaea are ubiquitous [6] and may therefore represent a significant component of global ammonia oxidation. 3. Biochemistry 4. Atmospheric chemistry The biochemistry of archaeal ammonia oxidation is unique and remains unresolved. AOA share genes only distantly related to those encoding the ammonia monooxygenase (AMO) of AOB. Currently there is no evidence that the product of ammonia oxidation by AOA is hydroxylamine and in fact it has been suggested that nitroxyl could instead be the product [7]. Nitroxyl may then be oxidised to nitrite via a nitroxyl oxidoreductase [8]. Furthermore Nitric oxide may also be important in the archaeal ammonia oxidation process. AOA generate large amount of greenhouse gases such as methane and nitrous oxide [5]. Furthermore Nitrosopumilus maritimus has been demonstrated to be a biological source of methylphosphonate. This could explain part of the high concentration of methane in surface oceans [9]. 5. Future research Rapid development of genome sequencing will allow more efficient comparative studies permitting for a greater understanding of the evolutionary origins of AOA. Furthermore, future research should focus on furthering our understanding on AOA biochemistry. Figure captions Figure 1: Schematic of the global N cycle [1] Figure 2: Archaeal amoA genes compared to bacterial amoA genes in a range of topsoils and deeper soil layers[10] Figure 1: Nitrogen cycle [ref. 1] Figure 2: AOA & AOB abundance [ref. 10]
  • 2. References: [1] Schleper, C. (2008). Metabolism of the deep. Nature, 456, 712-714. [2] Zhang, L. M., Offre, P. R., He, J. Z., Verhamme, D. T., Nicol, G. W., & Prosser, J. I. (2010). Autotrophic ammonia oxidation by soil thaumarchaea. Proceedings of the National Academy of Sciences, 107, 17240-17245. [3] Könneke, M., Bernhard, A.E., José, R., Walker, C.B., Waterbury, J.B., & Stahl, D.A. (2005). Isolation of an autotrophic ammonia-oxidizing marine archaeon. Nature, 437, 543-546. [4] Brochier-Armanet C, Boussau B, Gribaldo S, & Forterre P. (2008). Mesophilic Crenarchaeota: proposal for a third archaeal phylum, the Thaumarchaeota. Nat. Rev. Microbiol., 6, 245–52 [5] Stahl, D.A., & de la Torre, J.R. (2012). Physiology and diversity of ammonia-oxidizing archaea. Annual review of microbiology, 66, 83-101. [6] Offre, P., Spang, A., & Schleper, C. (2013). Archaea in biogeochemical cycles. Annu. Rev. Microbiol, 67, 437–57. [7] Walker CB ,de la Torre JR ,Klotz MG, Urakawa H,Pinel N,et al.(2010). Nitrosopumilus maritimus genome reveals unique mechanisms for nitrification and autotrophy in globally distributed marine crenarchaea. Proc. Natl. Acad. Sci., 107, 8818–23 [8] Hatzenpichler, R. (2012). Diversity, Physiology, and Niche Differentiation of Ammonia- Oxidizing Archaea. Applied and Environmental Microbiology. 78,21, 7501–7510. [9] Reeburgh W.S. (2007). Oceanic methane biogeochemistry. Chem. Rev., 107,486–513. [10] Leininger S, Urich T, Schloter M, Schwark L, Qi J, et al. (2006). Archaea predominate among ammonia-oxidizing prokaryotes in soils. Nature, 442, 806–9.