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Alcohlic Fermentation
Presented by:- Bushra
Presented to:- Sir Shehroz khan
Topic
•It is a common type of fermantaion
•It is used in baverages and bakeries product.
• C3H403 C2H50H + CO2
• (Pyruvic acid) (Alcohol)
Alcohlic Fermentation
• The enzyme alcohol dehydrogenase plays a central role in the most ancient form of
biotechnology: alcoholic fermentation.
•Yeast and many bacteria produce alcohol dehydrogenases. These microbial enzymes
catalyze the last step in the conversion of food into metabolic energy, creating ethanol.
•Sugars are broken down and used for energy, forming ethanol as the waste
product, which is excreted into the liquid surrounding the cell.
Making Alcohol
•We have harnessed this process to produce alcoholic beverages: yeast is allowed to
ferment grain sugars to form beer, and yeast is allowed to ferment grape juice to form
wine.
•Alcohol dehydrogenases in microbes function as tetramers.
•They are zinc-containing enzymes that utilize glucose.
Making Alcohol
•Each glucose molecule is broken down in a 10-step process called glycolysis.
•The product of glycolysis is two three-carbon sugars, called pyruvates, and ATP
(adenosine triphosphate).
• The two pyruvates are then converted into ethanol and carbon dioxide.
Making Alcohol
The overall process of fermentation is to convert glucose sugar to alcohol and carbon dioxide gas:
C6H12O6  2 CH3CH2OH + 2 CO2
sugar alcohol carbon dioxide gas
(glucose) (ethyl alcohol or ethanol)
Making Alcohol
Making Alcohol
Making Alcohol
Breaking Down Alcohol
Alcohol dehydrogenase
CH3CH2OH + 2 NAD  CH3CHO + 2 NADH
alcohol cofactor aldehyde cofactor
(ethanol) (acetaldehyde)
Acetaldehyde dehydrogenase 2
CH3CHO + H2O  CH3COOH
aldehyde
(acetaldehyde) (acetic acid)
Breaking Down Alcohol
Acetaldehyde dehydrogenase 2
CH3CHO + H2O  CH3COOH
aldehyde
(acetaldehyde) (acetic acid)
The acetic acid can be used to form fatty acids (watch that waistline!), or it can
be further broken down into CO2 and water.
Alcohol dehydrogenase provides a line of defense against a common toxin in
our environment.
•Alcohol dehydrogenase also modifies other alcohols, sometimes producing
even more dangerous products:
•Methanol, which is commonly used to “denature” ethanol rendering it
undrinkable, is converted to formaldehyde by alcohol dehydrogenase.
Dangers of Alcohol
• The formaldehyde then causes severe damage, attacking proteins and
embalming them.
•Small amounts of methanol cause blindness, as the sensitive proteins in the
retina are attacked, and larger amounts, perhaps a glassful, lead to widespread
damage and death.
Dangers of Alcohol
Breaking Down Methanol
Alcohol dehydrogenase
CH3OH + 2 NAD  CH3CHO + 2 NADH
alcohol cofactor aldehyde cofactor
(methanol) (formaldehyde)
Alcohol dehydrogenase
CH3CH2OH + 2 NAD  CH3CHO + 2 NADH
alcohol cofactor aldehyde cofactor
(ethanol) (acetaldehyde)
• Our bodies create at least nine different forms of alcohol
dehydrogenase, each with slightly different properties.
• Most of these are found primarily in the liver, including the 3 form
• The form is found in the lining of the stomach.
• Each enzyme is composed of two subunits.
• Ethanol is not the only target or substrate of these enzymes, they also make
important modifications to retinol, steroids, and fatty acids.
Structure & Function
Alcohlic Fermentation by Bushra

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Alcohlic Fermentation by Bushra

  • 2. Alcohlic Fermentation Presented by:- Bushra Presented to:- Sir Shehroz khan Topic
  • 3. •It is a common type of fermantaion •It is used in baverages and bakeries product. • C3H403 C2H50H + CO2 • (Pyruvic acid) (Alcohol) Alcohlic Fermentation
  • 4. • The enzyme alcohol dehydrogenase plays a central role in the most ancient form of biotechnology: alcoholic fermentation. •Yeast and many bacteria produce alcohol dehydrogenases. These microbial enzymes catalyze the last step in the conversion of food into metabolic energy, creating ethanol. •Sugars are broken down and used for energy, forming ethanol as the waste product, which is excreted into the liquid surrounding the cell. Making Alcohol
  • 5. •We have harnessed this process to produce alcoholic beverages: yeast is allowed to ferment grain sugars to form beer, and yeast is allowed to ferment grape juice to form wine. •Alcohol dehydrogenases in microbes function as tetramers. •They are zinc-containing enzymes that utilize glucose. Making Alcohol
  • 6. •Each glucose molecule is broken down in a 10-step process called glycolysis. •The product of glycolysis is two three-carbon sugars, called pyruvates, and ATP (adenosine triphosphate). • The two pyruvates are then converted into ethanol and carbon dioxide. Making Alcohol
  • 7. The overall process of fermentation is to convert glucose sugar to alcohol and carbon dioxide gas: C6H12O6  2 CH3CH2OH + 2 CO2 sugar alcohol carbon dioxide gas (glucose) (ethyl alcohol or ethanol) Making Alcohol
  • 10. Breaking Down Alcohol Alcohol dehydrogenase CH3CH2OH + 2 NAD  CH3CHO + 2 NADH alcohol cofactor aldehyde cofactor (ethanol) (acetaldehyde) Acetaldehyde dehydrogenase 2 CH3CHO + H2O  CH3COOH aldehyde (acetaldehyde) (acetic acid)
  • 11. Breaking Down Alcohol Acetaldehyde dehydrogenase 2 CH3CHO + H2O  CH3COOH aldehyde (acetaldehyde) (acetic acid) The acetic acid can be used to form fatty acids (watch that waistline!), or it can be further broken down into CO2 and water.
  • 12. Alcohol dehydrogenase provides a line of defense against a common toxin in our environment. •Alcohol dehydrogenase also modifies other alcohols, sometimes producing even more dangerous products: •Methanol, which is commonly used to “denature” ethanol rendering it undrinkable, is converted to formaldehyde by alcohol dehydrogenase. Dangers of Alcohol
  • 13. • The formaldehyde then causes severe damage, attacking proteins and embalming them. •Small amounts of methanol cause blindness, as the sensitive proteins in the retina are attacked, and larger amounts, perhaps a glassful, lead to widespread damage and death. Dangers of Alcohol
  • 14. Breaking Down Methanol Alcohol dehydrogenase CH3OH + 2 NAD  CH3CHO + 2 NADH alcohol cofactor aldehyde cofactor (methanol) (formaldehyde) Alcohol dehydrogenase CH3CH2OH + 2 NAD  CH3CHO + 2 NADH alcohol cofactor aldehyde cofactor (ethanol) (acetaldehyde)
  • 15. • Our bodies create at least nine different forms of alcohol dehydrogenase, each with slightly different properties. • Most of these are found primarily in the liver, including the 3 form • The form is found in the lining of the stomach. • Each enzyme is composed of two subunits. • Ethanol is not the only target or substrate of these enzymes, they also make important modifications to retinol, steroids, and fatty acids. Structure & Function