Affinity chromatography

1. Affinity
Chromatography
By......
Nandini Parkavi .S
15BBT028

2. Affinity Chromatography.....
• Separating biochemical mixtures based on a highly
specific interaction between antigen and antibody,
enzyme and substrate, or receptor and ligand.

3. Principle
• Stationary phase - mobile phase
• The target molecule becoming trapped on a stationary
phase
• The other molecules in the mobile phase
• Elution

4. Affinity chromatography can be
used to:
• Purify and concentrate a substance from a mixture into a
buffering solution
• Reduce the amount of unwanted substances in a mixture
• Discern what biological compounds bind to a particular
substance
• Purify and concentrate an enzyme solution.

5. Antigen - Antibody

6. Purification of mouse IgG1
Mouse IgG1 binds strongly to protein G

7. Polyclonal antibody