Adaptation through Livelihood Diversification & Irrigation: A Case Study of Agro-Pastoralist Youth in South Omo, Ethipoia – Sustainable solutions to water and climate challenges I – 2023 Water for Food Global Conference.pptx
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Livestock destocking have been started in 1980s by NGOs and state agencies (Slaughter destocking related to meat supply during drought
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Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
The increased availability of biomedical data, particularly in the public domain, offers the opportunity to better understand human health and to develop effective therapeutics for a wide range of unmet medical needs. However, data scientists remain stymied by the fact that data remain hard to find and to productively reuse because data and their metadata i) are wholly inaccessible, ii) are in non-standard or incompatible representations, iii) do not conform to community standards, and iv) have unclear or highly restricted terms and conditions that preclude legitimate reuse. These limitations require a rethink on data can be made machine and AI-ready - the key motivation behind the FAIR Guiding Principles. Concurrently, while recent efforts have explored the use of deep learning to fuse disparate data into predictive models for a wide range of biomedical applications, these models often fail even when the correct answer is already known, and fail to explain individual predictions in terms that data scientists can appreciate. These limitations suggest that new methods to produce practical artificial intelligence are still needed.
In this talk, I will discuss our work in (1) building an integrative knowledge infrastructure to prepare FAIR and "AI-ready" data and services along with (2) neurosymbolic AI methods to improve the quality of predictions and to generate plausible explanations. Attention is given to standards, platforms, and methods to wrangle knowledge into simple, but effective semantic and latent representations, and to make these available into standards-compliant and discoverable interfaces that can be used in model building, validation, and explanation. Our work, and those of others in the field, creates a baseline for building trustworthy and easy to deploy AI models in biomedicine.
Bio
Dr. Michel Dumontier is the Distinguished Professor of Data Science at Maastricht University, founder and executive director of the Institute of Data Science, and co-founder of the FAIR (Findable, Accessible, Interoperable and Reusable) data principles. His research explores socio-technological approaches for responsible discovery science, which includes collaborative multi-modal knowledge graphs, privacy-preserving distributed data mining, and AI methods for drug discovery and personalized medicine. His work is supported through the Dutch National Research Agenda, the Netherlands Organisation for Scientific Research, Horizon Europe, the European Open Science Cloud, the US National Institutes of Health, and a Marie-Curie Innovative Training Network. He is the editor-in-chief for the journal Data Science and is internationally recognized for his contributions in bioinformatics, biomedical informatics, and semantic technologies including ontologies and linked data.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Adaptation through Livelihood Diversification & Irrigation: A Case Study of Agro-Pastoralist Youth in South Omo, Ethipoia – Sustainable solutions to water and climate challenges I – 2023 Water for Food Global Conference.pptx
2. Background: Ethiopian Lowlands
● Increasing frequency of drought and
other natural disasters - flooding,
desert locust infestation
● Traditional rangeland management
and governance system has
weakened
● Limited infrastructure and weak
markets (minimal commercial
orientation)
→ threatening the viability and sustainability
of pastoralism
→ leading more pastoralists to decrease
their reliance on livestock and agricultural
production for sustenance and income
generation
→ changing livelihood system –higher
diversification
2
3. Background: Resilience in Pastoral Areas (RIPA)
Project
WHO: iDE is implementing a USAID Feed the Future
project with other partners - Global Communities and
GOAL
WHERE: lowlands of Ethiopia, inhabited by pastoralists
and agro-pastoralists
WHAT: improving agro-pastoralists’ resilience - ability to
recover, adapt, and mitigate against the effects of
climate change
OUTCOME: improve the lives of people transitioning out
of pastoralism (ToPs) by maximizing their livelihood
diversification and financial inclusion opportunities
3
4. Our Approach: Human-Centered Design
4
Solution-focused - focused on designing a solution
with the end-user
Empathy based - gathers insights that understand
why someone conducts a behavior
Iterative - celebrates changing the design approach,
tools, techniques to best understand the problem
and adjust solutions
Creative - continuously creating and making low-
high fidelity solutions
Leading with people to design to context
Solutions
DESIRABILITY
VIABILITY
FEASIBILITY
Solutions
5. Our Approach: Human-Centered Design
5
Currently piloting youth
engagement in different Income
generating activities (IGAs) and
value chains
Understand agro-pastoral
youths’ latent needs,
challenges, motivations, and
aspirations, and the barriers
and opportunities in market
system
Ideated, designed, and tested more
sustainable and market based
livelihood opportunities
DISCOVER EXPERIMENT DELIVER
8. Youth Group in Dassanech, South Omo
Name of the
group
Harif Commercial Fodder Producer
Enterprise
Location Dasenech woreda, Lobet kebele
Group
formation date
01 July 2020
Total members 34 (20 Female and 14 Male)
Monthly
savings
50 ETB per member per
month group saving (~$1)
Crops Panic (Panicum maximum) and
Rhodes grass
9. Youth Group in Dassanech, South Omo
Average profit per youth per sale
$117
From the total production, 70% of
the fodder is sold, while remaining
30% is used to feed own livestock
5 harvests since January, 2021
Harvest Seed
sales
(kg)
Profit
(USD)
Herbage
sales
(bales)
Profit
(USD)
1st – – – –
2nd 233 1,553 958 1,804
3rd 255.5 1,988 1150 2,044
4th 300 2,000 1700 3,400
5th 225 1,500 850 1,700
10. ● Prevents livestock death or offtaking measures, can
maintain livestock health
● Contributes to family food security and nutrition
(maintain key nutrient sources)
● Generates income to support a changing lifestyle which
requires purchase of more goods, less reliance on own
cereal and livestock production
● Increases entrepreneurship and market connectivity
through producing and selling to customers
● Decreases dependency on government and organizations
for aid; addresses free hand-out mentality
Impact
11. Lessons Learned
Working on existing experience: piloted fodder production in
previous project, allowing for development of partnership with
research center, selection of best species, and improved
practices
Collaboration with a research institution: research
organization's technical support and co-investment was
essential for the effective startup of commercial fodder
production
Strong government support: The woreda agriculture office
gave commercial fodder-producing organizations land,
irrigation water access, and technical assistance
12. What's Next
Potential for seed certification for multiplication: extensive process that requires supervision
throughout all stages and meeting stringent requirements - could allow youth support to scale up
and sell to out growing companies
Reduce post-harvest loss: Develop seed and fodder storage facilities - help extend shelf life and
increase transportability
Improve irrigation water management: managed by local government Water, Mineral and Energy
office - address issues with administration to improve access and use
Improve pump use & maintenance: pumps often out of operation for more than 10 days a month
due to a lack of fuel, spare parts, and skilled workers to perform repairs - improve training for fodder
producers and other youth
Explore crop rotation and intercropping: pilot rotating and/or intercropping fodder species with
other nitrogen fixing crops
Editor's Notes
Introduce myself - I’m a senior Designer working with iDE - International Development Enterprises, in our Ethiopia country office
Today I’d like to share with you about how iDE is working with agro-pastoralist youth in the lowlands of Ethiopia to mitigate against the effects of climate change on their livelihood through irrigated fodder production
Dr. tsegaye has already shared about the current drought situation in Ethiopia and Kenya, so I’ll share a few more specifics about the area where iDE is implementing a USAID funded project. We are currently working across the whole region shown in this map, but the case study I am sharing today focuses on South Omo zone here in blue. There is a large river, called Omo River, which runs through the region and empties into Lake turkana, where Ethiopia share a portion of the lake with Kenya
between June and December of 2022, the project implementation area , experienced one of the worst droughts recorded in 40 years in the Horn of Africa.
In south Omo Zone, more than 300k people of the zone have been severely affected by drought
14,000 livestock have died over the past 6 months and over 3 million livestock need urgent water and fodder.
Over the past several years, the area has also experienced annual flooding, the river overflow causes damage to crops while also displacing people near the river
In addition to the effects of natural disasters, S.Omo pastoralists are also affected by a weakening traditional rangeland management system, especially as ethnic groups engage in conflict over resources, and they are also affected by weak infrastructure and markets
So in general, the confluence of these factors are affecting the viability and sustainability of agro-pastoralists’ livelihood
Since they are not able to maintain their herds or grow cereal crops as they used to, the younger generations are being pushed to change or diversify their livelihood
So that brings us to the project iDE is implementing in this region - the Resilience in Pastoral Areas, or RIPA project. It has multiple components - including improving disaster risk management and rangeland management strategies, improving food insecurity through nutrition initiatives, and improving livestock and crop production systems.
iDE is mainly focusing on engaging youth to diversify their livelihood opportunities, in order to generate income from other natural resources and activities which are somewhat less climate dependent
Why youth?
While the younger generation grew up in families with large herd sizes and less climatic shocks, now they are increasingly facing higher frequency of droughts, natural shocks, growing population pressure
In the past when drought hit, their families would rely on government or NGO handouts of food and fodder, then would continue with previous rangeland management, sometimes having to travel further for water and grass
this model is no longer sustainable bc of the severity of today’s droughts, and because it breeds dependency rather than encouraging adaptation or new approaches
**Using a market based approach, iDE is facilitating youth to adapt and diversify their agro-pastoral livelihood system - so they can manage and use critical resources like water in a different way AND and at the same time generate income
Pastoral communities have often been misunderstood - previous government or NGO approaches have often been based on a highland agricultural practices and knowledge
We used Human-Centered Design to better understand the unique challenges and opportunities that youth face in adapting and changing their livelihood system
Without going into too much detail on HCD, it is essentially a collaborative innovation methodology which allows us to design context-specific solutions, and lead with solutions, not problems
So, rather than only focusing on drought as a problem, in this project, we tried to understand the youth journey and experience of adaptation and diversification
We started by exploring youths’ needs, challenges, motivations, and also uncovering the barriers and opportunities in the environment and the market system
We then developed ideas and potential business models with community members through a co-design process
And currently, we are engaging youth in various IGAs and value chains
→ One of the main IGAs we are engaging youth in is irrigated commercial fodder production due to the presence of the Omo River in their area, the dire need for livestock feed, and youth’s interest and openness towards trying irrigated agriculture (a new practice)
Youth were organized in groups and registered as a youth enterprise, youth started saving in a shared savings account, which they use to purchase inputs
iDE connected youth to a regional research center to access improved fodder seeds which are appropriate for the agro-ecology in the area, and to be trained in appropriate land preparation, seeding, management, irrigation, and harvest practices
Using diesel pump to access water from Omo River and using furrow irrigation practice, watering about once per week
Mainly growing Panicum and Rhodas because it is appropriate for the area as well as fast-growing - the youth are able to harvest after ~45 days, meaning they can also produce multiple times throughout the year
They manage the production and harvest practices as a group on land designated by the government
The frequency of irrigation depends on the availability of water, the fodder producers irrigate the crop 1 a week and the time required to irrigate may be 7 hours for 4 hectare of land
From the production, they are feeding their own livestock, selling fodder to other agro-pastoralists in local market; in some cases selling to iDE and other NGOs to redistribute as part of crisis response activities
Introduce to a local group producing fodder for the past several years to give some insight into their practice and how they are producing and generating income thus far
This group formed in 2020, and includes both male and female members, which share tasks and responsibilities
Youth are earning profit from selling both the herbage and seed - linked to local traders as well as to some private companies (mainly for seed)
Use about 30% of herbage to feed own livestock remaining 70% the sell
*graph is showing amount sold, not total production
Cash from sales is used to cover HH goods, school fees, etc.
Some profit saved in the group’s bank account at MFI and re-invested in the enterprise
40% production of fodder sold at local market to cover the cost of food and other basic 40% production of fodder sold at local market to cover the cost of food and other basic needs, 30% of fodder consumed own livestock and the rest 30% sold as bele needs , 30% of fodder consumed own livestock and the rest 30% sold as bale
**this has a significant impact on their livelihoods, bc on one hand it’s allowing youth to continue raising livestock and benefit from it as a source of food and asset base
On the other hand, it’s generating income to cover costs that they previously might not have had but now need to adapt to given the changing lifestyle
**iDE’s market based approach to fodder production is supporting youth to adapt in a way that is desirable for them and is also appropriate for the changing environment and natural resources available because it’s using the nearby river for irrigation and reducing dependence on traveling far distances for fodder