This study examined the relationship between acculturation and knowledge of health harms and benefits of smoking among Latinos in Minnesota. A survey of 804 Latino adults found high knowledge that smoking causes lung cancer and heart disease, though smokers had less knowledge of heart disease risks. More acculturated respondents knew less common health links. Smokers perceived more smoking benefits than non-smokers, and greater acculturation predicted perceiving more benefits. The study used multidimensional acculturation measures to better understand how culture influences beliefs about smoking.