Abstract. Bissenbay Akerke
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The document discusses using methylene blue (MB) to treat perinatal brain damage caused by hypoxic-ischemic events. In vitro cell culture studies showed MB has a protective effect on neuronal progenitor and oligodendrocyte cell lines by inhibiting reactive oxygen species production and restoring ATP levels. However, in vivo studies using an animal model of neonatal hypoxic-ischemic injury did not provide conclusive results due to time limitations. While cell culture studies showed promising results, further research is still needed to determine MB's exact mechanisms of action and effectiveness in treating different types of perinatal brain injuries.
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This document summarizes a study on developing a novel protein interaction platform to study neurodegeneration. The study cultured neuronal stem cells to form neurospheres, which were used as a model system. Lentiviral transfection was optimized to introduce target genes stably into the neurospheres. The goal was to analyze protein interactions of Alzheimer's disease proteins by co-immunoprecipitation of neurosphere cultures expressing tagged target proteins. This model aims to further the understanding of molecular mechanisms in neurodegenerative disorders like Alzheimer's disease.luisetto m. brain response in some systemic immune condition-toxicological as...
luisetto m. brain response in some systemic immune condition-toxicological as...M. Luisetto Pharm.D.Spec. Pharmacology
The document discusses the relationship between the immune system and brain conditions from a toxicological perspective. It summarizes several studies that found:
1) The immune system plays an important role in brain development and any immune activation during development can affect later neural function, immune function, mood and cognition.
2) A drug called fingolimod that modulates sphingosine-1-phosphate receptors, reducing lymphocyte migration from lymph nodes, significantly reduced relapse rates and disability progression in multiple sclerosis patients compared to placebo.
3) Systemic immune status can influence local brain tissue conditions, and this effect could be considered a type of toxicological effect worth further investigation to better understand disease pathogenesis and develop new pharmacologicalCambridge Scientist Comes Close to Multiple Sclerosis Cure
A community-based neurological practice in Charlotte, NC, The Neurological Institute is dedicated to improving patient care. In addition, The Neurological Institute prioritizes medical education and research in Charlotte, NC. The research in question focuses on conditions such as Parkinson’s disease, Alzheimer’s disease, and multiple sclerosis (MS).
Senior Semester Final Project Poster
1. Alzheimer's disease is a major health concern that causes cognitive decline through β-amyloid accumulation and plaque formation in the brain.
2. Resveratrol has been shown to inhibit β-amyloid accumulation by binding to the proteins and changing their conformation to a non-toxic form, reducing plaque formation and neuronal damage.
3. While studies support resveratrol's potential as a therapeutic for Alzheimer's disease, long-term clinical trials in humans are still needed to confirm its safety and efficacy.
Oligodendrocytes As Regulators Of Neuronal Networks During Early Postnatal De...
Taruna Ikrar, MD., PhD (Coauthor Oligodendrocytes as Regulators of Neuronal Networks during Early Postnatal Development)
SSC4a Report (final)
This study investigated the effects of noradrenaline (NA) on human B cells in vitro to explore its potential role in post-stroke infection. Peripheral blood mononuclear cells were isolated from healthy volunteers and cultured with varying concentrations of NA. Flow cytometry was used to identify and analyze B cell subpopulations. The results showed that higher NA concentrations increased the total number of B cells but decreased their viability. NA also significantly reduced the number of marginal zone B cells, which are important for innate immune defense, at 48 hours. This suggests that NA exposure may impair B cell function and contribute to post-stroke immunosuppression and increased risk of infection through its effects on marginal zone B cells and other innate-like lymphocytes.
Mitochondrial aging 10.04.2019
Mitochondria are organelles found in cells that are involved in biochemical processes like respiration and energy production. Mitochondrial dysfunction increases during aging and age-related disorders. The crosstalk between mitochondrial biogenesis, which generates new mitochondria, and mitophagy, which eliminates damaged mitochondria, is important for maintaining mitochondrial health and cellular homeostasis in response to physiological and environmental cues. Exercise can stimulate mitochondrial biogenesis and mitophagy through activation of AMPK signaling pathways, and may help treat various diseases by modulating mitochondrial dynamics proteins.
Ms preventable & strategies for remission
This document discusses the causes and treatment of multiple sclerosis from a holistic perspective. It argues that MS and other chronic autoimmune diseases are caused by a combination of dietary deficiencies, viruses, and bacteria interacting in the gut. It summarizes research showing how gut bacteria can trigger autoimmune responses. The document advocates treating and preventing MS through diet, exercise, plant medicine, and focusing on gut health in order to strengthen immunity. Sugar and processed foods are identified as inflammatory and as providing an environment for harmful bacteria and viruses to thrive.
Cv feb 2016
• Extensive research experience in ocular translational research in uveitis, retinal ischemia, glaucoma, neurodegenerative disease, and retrocorneal fibrosis.
• In-depth knowledge and wet laboratory experience from basic science to translational research: Cellular/molecular biology, Microbiology, Pathology, Biochemistry, Neuroimmunology, in vivo rodent translational research.
• Independent self-motivated scientist working for multiple research projects from perform experiments, analyze data, generate manuscripts, grant, or oral presentations
• Experience in managing lab, collaboration, supervising/mentoring students and fellows.
Baker ectrims teaching slides
This document summarizes a presentation given by Professor David Baker on B cell therapy for multiple sclerosis. It discusses B cell subsets, the role of B cells in the pathogenesis of MS, response to different therapies as a key experiment to understand biology, and repopulation characteristics of immune cells after treatment. It also provides diagrams on immune cell differentiation and repopulation, the two compartment model of treating inflammatory lesions in MS, phenotypes seen in active MS lesions, and types of monoclonal antibodies used as treatments.
Austism CBD
Immune system dysregulation is well-recognized in autism and thought to be part of the etiology of this disorder. The endocannabinoid system is a key regulator of the immune system via the cannabinoid receptor type 2 (CB2R) which is highly expressed on macrophages and microglial cells. We have previously published significant differences in peripheral blood mononuclear cell CB2R gene expression in the autism population. The use of the Gc protein-derived Macrophage Activating Factor (GcMAF), an endogenous glycosylated vitamin D binding protein responsible for macrophage cell activation has demonstrated positive effects in the treatment of autistic children. In this current study, we investigated the in vitro effects of GcMAF treatment on the endocannabinoid system gene expression, as well as cellular activation in blood monocyte-derived macrophages (BMDMs) from autistic patients compared to age-matched healthy developing controls.
Conclusions
This study presents the first observations of GcMAF effects on the transcriptionomics of the endocannabinoid system and expression of CB2R protein. These data point to a potential nexus between endocannabinoids, vitamin D and its transporter proteins, and the immune dysregulations observed with autism.
"Bioluminescent Imaging of Histidyl-Transfer RNA Synthetase-Induced Myositis ...
My talk from the American College of Rheumatology Meeting in Boston, MA, November 14-19, 2014: • "Bioluminescent Imaging of Histidyl-Transfer RNA Synthetase-Induced Myositis Reveals Early-Phase Involvement of NF-kB-Mediated Inflammation".
PLOS one 2015
This research article characterizes cancer stem cells derived from MYCN-dependent medulloblastoma (MB), a type of pediatric brain tumor. The researchers established tumor-derived neurosphere cell lines from a genetically engineered mouse model of MB driven by MYCN overexpression. They found that a fraction of these neurospheres were growth factor independent, expressed the stem cell marker CD133, failed to differentiate when MYCN was withdrawn, and were highly tumorigenic when implanted in mouse brains. Treatment with an aurora kinase inhibitor that degrades MYCN extended survival in mice with MB allografts, suggesting it may convert the cancer stem cells to a less aggressive state. The study demonstrates MYCN plays a critical role in expansion and
Luisetto m et al (2017) immune shock chronologic event in some brain patho...
Luisetto m et al (2017) immune shock chronologic event in some brain patho...M. Luisetto Pharm.D.Spec. Pharmacology
Elevated levels of the cytokine interleukin-6 (IL-6) have been found in the brains and cerebrospinal fluid of individuals with autism spectrum disorder. A review of literature found that IL-6 plays an important role in central nervous system development and is normally expressed at low levels, but chronic over-expression of IL-6 in mice causes neurological alterations. Studies also found IL-6 was significantly increased in the cerebellum of autistic patients compared to controls. Additionally, over-expressing IL-6 in cerebellar granule cells in vitro impaired cell adhesion and migration and stimulated excessive formation of excitatory synapses, suggesting elevated IL-6 may contribute to autism pathogenesis by disrupting neuralDiapos seminario microglía
Microglia-Derived Exosomal microRNA-151-3p Enhances Functional Healing After Spinal Cord Injury by Attenuating Neuronal Apoptosis via Regulating the p53/p21/ CDK1 Signaling Pathway
Similar to Abstract. Bissenbay Akerke (20)
Ameliorating mitochondrial dysfunction as a treatment of perinatal
Ameliorating mitochondrial dysfunction as a treatment of perinatal
Development and implementation of a novel interactome platform in studies on ...
Development and implementation of a novel interactome platform in studies on ...
luisetto m. brain response in some systemic immune condition-toxicological as...
luisetto m. brain response in some systemic immune condition-toxicological as...
Cambridge Scientist Comes Close to Multiple Sclerosis Cure
Cambridge Scientist Comes Close to Multiple Sclerosis Cure
Oligodendrocytes As Regulators Of Neuronal Networks During Early Postnatal De...
Oligodendrocytes As Regulators Of Neuronal Networks During Early Postnatal De...
Stem_cell_therapies_for_perinatal_brain_injuries-libre
Stem_cell_therapies_for_perinatal_brain_injuries-libre
"Bioluminescent Imaging of Histidyl-Transfer RNA Synthetase-Induced Myositis ...
"Bioluminescent Imaging of Histidyl-Transfer RNA Synthetase-Induced Myositis ...
Luisetto m et al (2017) immune shock chronologic event in some brain patho...
Luisetto m et al (2017) immune shock chronologic event in some brain patho...
Abstract. Bissenbay Akerke
- 1. Ameliorating mitochondrial dysfunction as a treatment of perinatal brain damage Akerke Bissenbay, BSc; Anton Kichev, PhD. Perinatal Brain Injury Group, King’s College London Abstract Brain injuries occurring during perinatal period are caused mainly by hypoxic-ischemic (HI) events and/or inflammation and make up 70% in the overall child mortality and disabilities. HI induced injury develops through complex cascade of pathophysiological processes with the activation of both necrotic and apoptotic cell death leading to damage and death of neurons and glial cells. During that processes mitochondrial dysfunction plays a primary role. Despite the advances in the neonatal care in the last years the treatment of perinatal HI injury is still limited. There is thus, an obvious need for more effective treatment strategies and mitochondria- targeted treatments are promising candidates for research. Increasing body of evidence is accumulating about the beneficial role of Methylene Blue (MB) treatment for improving the mitochondrial function and cell survival in HI conditions. Our in vitro cell culture studies confirmed for the first time the potential protective effect of MB on neuronal progenitor and oligodendrocyte cell lines. The protective effect of MB was mediated through the inhibition of reactive oxygen species (ROS) production by MB in both cell lines and restoration of hampered ATP levels in oligodendrocyte precursor cell line during HI. In vitro studies with microglial cell lines did not identified MB inhibitory role for microglial cells activation under inflammatory conditions as previously suggested. In vivo studies with animal model of neonatal HI treated with MB did not give conclusive results due to time limitations of this project. Despite the promising results obtained with cell culture studies, there are still many unanswered questions regarding the exact mechanism of MB action and its effect on HI induced perinatal brain injury. Further studies are needed in order to determine the potential beneficial role of MB treatment in the context of different perinatal brain injuries and main mechanisms by which MB protects neuronal/glial cells.