This study examines the protective effects of Methylene Blue (MB) on neuronal and glial cells exposed to oxygen-glucose deprivation (OGD), a model of hypoxic-ischemic injury in the developing brain. Key findings include: 1) MB treatment after OGD exposure protected neuronal progenitor and oligodendrocyte precursor cells from cell death. 2) MB reduced reactive oxygen species production and restored ATP levels in neuronal progenitor cells after OGD. 3) MB treatment restored ATP production in oligodendrocyte precursor cells following OGD. The results suggest MB has a protective effect on brain cells during OGD-induced cell death by decreasing reactive oxygen species and restoring energy production through