A protein that was isolated by a team of university scientists is suspected to be expressed on a yet
to be identified lymphocyte. Outline a series of experiments that you would design to identify the
cells expressing the protein.
Solution
The protein expressing gene is identified on DNA. The gene of interest and the plamid are cut
with same restriction endonuclease. The obtained gene is inserted in the plasmid. The plasmid is
transformed into cell line. The transformed plasmid containing a marker gene such as antibiotic
resistance gene or LacZ is useful to screen the presence of newly transformed gene. Screening
for the presence of desired gene is done by hybridization or other molecular techniques. The
desired gene expression can be studied by gene knockout or RNA interference..
TỔNG HỢP HƠN 100 ĐỀ THI THỬ TỐT NGHIỆP THPT TOÁN 2024 - TỪ CÁC TRƯỜNG, TRƯỜNG...
A protein that was isolated by a team of university scientists is su.pdf
1. A protein that was isolated by a team of university scientists is suspected to be expressed on a yet
to be identified lymphocyte. Outline a series of experiments that you would design to identify the
cells expressing the protein.
Solution
The protein expressing gene is identified on DNA. The gene of interest and the plamid are cut
with same restriction endonuclease. The obtained gene is inserted in the plasmid. The plasmid is
transformed into cell line. The transformed plasmid containing a marker gene such as antibiotic
resistance gene or LacZ is useful to screen the presence of newly transformed gene. Screening
for the presence of desired gene is done by hybridization or other molecular techniques. The
desired gene expression can be studied by gene knockout or RNA interference.
