Water is essential for life due to its unique properties arising from hydrogen bonding between polar water molecules. Water's polarity allows it to dissolve many other polar substances and ions, acting as a universal solvent and transport medium in organisms. Water's high specific heat capacity and heat of vaporization enable important thermal regulation processes. In blood, glucose, amino acids, and ions are carried in the plasma due to their polarity, while fats and cholesterol require transport in lipoprotein complexes due to their nonpolar nature. Oxygen is only slightly soluble in water alone, and is mainly transported bound to hemoglobin in red blood cells. The document discusses these topics through examples and comparisons to methane.
these slides include the basic structure of water how they form bonds how they interact and its physical and chemical properties it also include the biochemical importance of water .
Title: Structure and Properties of Water
Introduction:
Water is a vital molecule for all forms of life. Its unique structure and properties are crucial for various biological, chemical, and physical processes. Understanding the molecular structure and the resulting properties of water can provide insight into its essential role in nature and science.
1. Molecular Structure of Water:
Water (H₂O) is composed of two hydrogen atoms covalently bonded to one oxygen atom. The oxygen atom is more electronegative than hydrogen, resulting in a polar covalent bond. This polarity creates a partial negative charge near the oxygen atom and a partial positive charge near the hydrogen atoms.
2. Geometry and Bond Angles:
Water has a bent or V-shaped molecular geometry due to the two lone pairs of electrons on the oxygen atom. The bond angle between the hydrogen-oxygen-hydrogen (H-O-H) atoms is approximately 104.5 degrees. This angle is less than the ideal tetrahedral angle of 109.5 degrees due to the repulsion between the lone pairs of electrons.
3. Hydrogen Bonding:
One of the most significant properties of water is its ability to form hydrogen bonds. Each water molecule can form up to four hydrogen bonds: two through its hydrogen atoms and two through the lone pairs on its oxygen atom. These hydrogen bonds are responsible for many of water's unique properties.
4. High Cohesion and Adhesion:
Cohesion refers to the attraction between water molecules, while adhesion refers to the attraction between water molecules and other surfaces. The hydrogen bonds contribute to water's high cohesion, which is why water has a high surface tension. This allows insects to walk on water and causes water droplets to form. Adhesion helps water to stick to other surfaces, which is important for processes like capillary action in plants.
5. High Specific Heat Capacity:
Water has a high specific heat capacity, meaning it can absorb a lot of heat energy before its temperature increases significantly. This property helps to moderate Earth's climate and allows organisms to maintain stable internal temperatures.
6. High Heat of Vaporization:
Water requires a significant amount of energy to transition from a liquid to a gas due to the strength of hydrogen bonds. This property is crucial for regulating temperature through processes like sweating and transpiration.
7. Density and Ice Formation:
The density of water decreases as it freezes. In liquid water, molecules are closely packed but still moving, whereas in ice, water molecules form a crystalline lattice that is less dense than liquid water. This is why ice floats on water, providing insulation for aquatic life in cold environments.
8. Solvent Properties:
Water is often referred to as the "universal solvent"
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
2. Understandings, Applications and Skills
Statement Guidance
2.2 U.1 Water molecules are polar and hydrogen
bonds form between them.
2.2 U.2 Hydrogen bonding and dipolarity explain
the cohesive, adhesive, thermal and
solvent properties of water.
Students should know at least one
example of a benefit to living
organisms of each property of water.
Transparency of water and maximum
density at 4°C do not need to be
included.
2.2 U.3 Substances can be hydrophilic or
hydrophobic.
2.2 A.1 Comparison of the thermal properties of
water with those of methane.
Comparison of the thermal properties
of water and methane assists in the
understanding of the significance of
hydrogen bonding in water.
2.2 A.2 Use of water as a coolant in sweat.
2.2 A.3 Modes of transport of glucose, amino
acids, cholesterol, fats, oxygen and sodium
chloride in blood in relation to their
solubility in water.
3. • A water molecule consists of an oxygen
atom covalently bound to two hydrogen
atoms
• Since O is more electronegative than H,
an unequal sharing of electrons occurs
• This creates a polar covalent
bond, with H having a partial positive
charge and O having a partial negative
charge
• The partial + charge is attracted to the
partial – charge creating an
intermolecular attraction between the
water molecules called a “Hydrogen
bond.”
• H-bonds are the strongest of the
intermolecular bonding, but is still
considered a weak bond; however since
there are so many H2O molecules they
give water its unique properties and
make it essential to life on this planet
2.2 U.1 Water molecules are polar and hydrogen bonds form between
them.
4. 2.2 U.2 Hydrogen bonding and dipolarity explain the cohesive, adhesive,
thermal and solvent properties of water.
Thermal Property
• Water has a high specific heat
capacity (amount of energy needed to
raise temperature of a substance by a
certain temperature level). Basically,
water can absorb a lot of heat and give
off a lot of heat without drastically
changing the temperature of water.
• Water’s high specific heat capacity results
from the extensive hydrogen bonding
between the water molecules.
• Water also has a high latent heat of
vaporization which means it takes a lot of
heat to evaporate water from a liquid to
a vapor. This is very important as a
cooling mechanism for humans. As we
sweat, the water droplets absorb heat
from our skin causing the water to
evaporate and our bodies to cool down.
http://community.thefoundry.co.uk/discussion/topic.aspx?f=9&t=77728
6. 2.2 A.1 Comparison of the thermal properties of water with those
of methane.
Methane
• waste product of
anaerobic respiration in
certain prokaryotes living
in anaerobic conditions
• Methane can be used as a
fuel
• If present in the
atmosphere it contributes
to the greenhouse effect.
Methane Water
Formula CH4 H2O
Molecular mass 16 18
Bonding Single covalent
Polarity nonpolar polar
Density (g cm-3) 0.46 1
Specific Heat Capacity
(J g-1 oc-1)
2.2 4.2
Latent heat of
vapourisation (J g-1)
760 2257
Melting point (oC) -182 0
Boiling point (oC) -160 100
https://upload.wikimedia.org/wikipedia/commons/5/55/3D_methane.PNG
https://commons.wikimedia.org/wiki/Water_molecule#mediaviewer/File:Water_molecule.svg
Key chemical property that causes
the major differences seen in the
physical properties.
Methanogenic prokaryotes
• can be found in swamps,
wetlands, the guts of
animals (including cattle
and sheep)
• can also be found in waste
dumps
7. 2.2 U.2 Hydrogen bonding and dipolarity explain the cohesive, adhesive,
thermal and solvent properties of water.
Cohesive Properties
• Water is a polar molecule, with a negative oxygen end and a positive hydrogen end.
• Hydrogen bonds that exist between water molecules create a high level of
attraction linking water molecules together. This attraction between two of the
same molecules is called cohesion.
• These cohesive forces allow water to move up vascular tissue in plants against
gravity. It also creates surface tension on water that allows some organisms to walk
on water.
http://www.kellyisola.com/tag/transformation-2/
8. 2.2 U.2 Hydrogen bonding and dipolarity explain the cohesive, adhesive,
thermal and solvent properties of water.
Adhesive Properties
• Not only does water bind
strongly to itself, it also forms H-
bonds with other polar
molecules. This is
called adhesion.
• This is an important property in
transpiration as well, as water
adheres to the cellulose in the
walls of the xylem vessels
• As water is evaporated from the
stomata, the adhesion can help
the water move up through the
xylem
Capillary Action
9. 2.2 U.2 Hydrogen bonding and dipolarity explain the cohesive, adhesive,
thermal and solvent properties of water.
Solvent Properties
• Water is known as the “universal solvent” because of its ability to dissolve many substances
because of its polarity.
• Water is able to dissolve other polar molecules such as many carbohydrates, proteins and
DNA; and positively and negatively charged ions such as Na+.
• This is essential because it allows water to act as a transport medium (blood and cytoplasm)
of important molecules in biological organisms
10. 2.2 U.3 Substances can be hydrophilic or hydrophobic.
• All substances that dissolve in water
are hydrophilic, including polar
molecules such as glucose, and
particles with positive or negative
charges such as sodium and chloride
ions.
• Substances that water adheres to,
cellulose for example, are also
hydrophilic.
http://www.middleschoolchemistry.com/img/content/multimedia/chapter_5/lesson_7/glucose.jpg
hydrophilic
( water loving )
This term is used to describe substances
that are chemically attracted to water.
A space filling molecular diagram of glucose
showing the positive and negative charges
11. • Molecules are hydrophobic if
they do not have negative or
positive charges and are
nonpolar
• All lipids are hydrophobic,
including fats and oils
• Hydrophobic molecules dissolve
in other solvents such as
propanone (acetone)
http://upload.wikimedia.org/wikipedia/commons/thumb/f/f1/Water_and_oil.jpg/450px-Water_and_oil.jpg
hydrophobic
( water fearing )
This term is used to describe substances that are
insoluble in water
2.2 U.3 Substances can be hydrophilic or hydrophobic.
12. 2.2 A.3 Modes of transport of glucose, amino acids, cholesterol, fats,
oxygen and sodium chloride in blood in relation to their solubility in
water.
Blood Plasma
• Blood transports many different
substances to different parts of the body
using a variety of methods
• Water is critical both as a solvent in which
many of the body's solutes dissolve
• In addition, due to its polarity water is
a great solvent of other polar molecules and
ions. This is vital because it allows water to
act as a transport medium (blood and
cytoplasm) of important molecules in
biological organisms.
Plasma 55%
• 91% Water
• 7% Blood Proteins
• 2% Nutrients (amino acids, sugars, lipids)
• Hormones and ions
Cellular Components 45%
• White Blood cells
• Red Blood Cells
13. http://4.bp.blogspot.com/71TuXJIWv8o/UChT59p73
fI/AAAAAAAAAFY/B1zkMgT-dlA/s1600/Glucose.png
Glucose
• Glucose is polar making it a
soluble molecule in water,
making it possible to be
transported in the blood
plasma
• Blood plasma consists
mainly of water (95%) plus
dissolved substances which
it transports.
2.2 A.3 Modes of transport of glucose, amino acids, cholesterol, fats,
oxygen and sodium chloride in blood in relation to their solubility in
water.
14. Amino acids
• Positive and negative charges (due to the amine and acid groups) therefore soluble in water
• R group varies, can be polar, non-polar or charged
• R group determines the degree of solubility
• carried by the blood plasma
There is an internal transfer of a hydrogen
ion from the -COOH group to the -NH2
group to leave an ion with both a
negative charge and a positive charge.
http://chemwiki.ucdavis.edu/Under_Construction/Chemguide_(Jim_Clark)/Properties_of_Organic_
Compounds/XIII._Amino_Acids_and_Other_Biochemistry/A._Amino_Acids/2._Acid-
Base_Reactions_of_Amino_Acids
2.2 A.3 Modes of transport of glucose, amino acids, cholesterol, fats,
oxygen and sodium chloride in blood in relation to their solubility in
water.
15. 2. Fats
• Large, non-polar molecules
• insoluble in water
• They are carried in blood inside
lipoprotein complexes (in the plasma)
1. Cholesterol
• molecules are hydrophobic, apart
• from a small hydrophilic region at
one end
• This is not enough to make
cholesterol dissolve in water
• They are carried in blood in
lipoprotein complexes (in the
plasma)
3. Lipoprotein complex
• Outer layer consists of single layer of
phospholipid molecules
• hydrophilic phosphate heads of the
phospholipids face outwards and are in
contact with water
• The hydrophobic hydrocarbon tails face
inwards and are in contact with the fats
• cholesterol molecules are positioned in the
phospholipid monolayer - hydrophilic region
facing outwards
• Proteins are also embedded in the
phospholipid layer (hence the name)
http://chienlab.wikispaces.com/file/view/lip
oprotein.jpg/45882185/lipoprotein.jpg
2.2 A.3 Modes of transport of glucose, amino acids, cholesterol, fats,
oxygen and sodium chloride in blood in relation to their solubility in
water.
16. O = O
Oxygen
• Non-polar molecule
• Due to the small size of an oxygen
molecule it is soluble in water, but
only just
• water becomes saturated with
oxygen at relatively low
concentrations
• As temperature increases the
solubility of oxygen decreases
• At body temperature (37 °C) very
little oxygen can be carried by the
plasma, too little to support aerobic
respiration
• hemoglobin in red blood cells carry
the majority of oxygen
• Hemoglobin has (4) binding sites for
oxygen
https://upload.wikimedia.org/wikipedia/commons/thumb/3/3d/1GZX_Haemoglobin
.png/480px-1GZX_Haemoglobin.png
Hemoglobin
2.2 A.3 Modes of transport of glucose, amino acids, cholesterol, fats,
oxygen and sodium chloride in blood in relation to their solubility in
water.
17. Sodium Chloride
• ionic compound
• freely soluble in water
• dissolving to form sodium ions
(Na+) and chloride ions (Cl-)
• carried in the blood plasma
http://www.northland.cc.mn.us/biology/Biology1111/animations/dissolve.swf
2.2 A.3 Modes of transport of glucose, amino acids, cholesterol, fats,
oxygen and sodium chloride in blood in relation to their solubility in
water.