Method Development for Visible
Spectrophotometric Analysis of Ibuprofen in Pharmaceuticals
Ibuprofen is a prominent member of the group of non-steroidal anti-inflammatory drugs (NSAIDs), with good anti-inflammatory
action, a very effective analgesic, with increased antipyretic effect. The aim of this research was to exactly quantify pure Ibuprofen content in tablets of a pharmaceutical, by a spectrophotometric analysis method in the Visible range. Ibuprofen was quantitatively converted to a bright orange dye
with a yellowish shade, by a color reaction with alpha-naphthylamine
in the presence of sodium nitrite, in an absolute ethanol medium. Following the analysis, it was found 397.952 milligrams of pure Ibuprofen content / film-coated tablet of the pharmaceutical product. This value was very close to Ibuprofen content declared by the pharmaceutical manufacturer (400 milligrams), with a mean deviation of only 0.512 percent from the officially declared amount of active substance. The value found fits perfectly within the normal limits provided by the European and International Pharmacopoeias standards, taken over by the Romanian Pharmacopoeia, 10th Edition. The spectrophotometric analysis method was then successfully subjected to statistical analysis.
Investigation of linearity, detection limit (LD) and quantitation limit(LQ) of active substance from pharmaceutical tablets
The aim of this research was to exactly quantify pure sodium metamizole from tablets , using a
spectrophotometric analysis in Visible range. The method applied has been subjected to a validation protocol which consisted in analyzing the following parameters: linearity of the method, detection limit (LD) ,
quantitation limit (LQ). Following actual dosing, pure sodium metamizole amount in tablet of pharmaceutical was found to be
477.477 mg assigned to a percentage content of 95.495 %, very close to official declared amount (500 mg), with an maximum average percentage deviation of only 4.505 % from the official declared active substance content. This value was situated below the maximum admissible percentage deviation from stated active substance content (± 5%), established by Romanian Pharmacopoeia, X-th Edition rules.
Visible Spectrophotometric Analysis Method of Sodium Metamizole in Tablets
The aim of this research was to exactly quantify pure sodium metamizole from tablets , using a
spectrophotometric analysis in Visible range. The method applied has been subjected to a validation protocol which consisted in analyzing the following parameters: linearity of the method, detection limit (LD) ,
quantitation limit (LQ), Sandell’s sensitivity, interference of excipients, stability of prepared solutions, method and system precision, accuracy of the method. Following actual dosing, pure sodium metamizole amount
in tablet of pharmaceutical was found to be 477.477 mg assigned to a percentage content of 95.495 %, very close to official declared amount (500 mg), with an maximum average percentage deviation of only 4.505 % from the official declared active substance content. This value was situated below the maximum admissible percentage deviation from stated active substance content (± 5%), established by Romanian Pharmacopoeia, X-th Edition rules.
PREPARATION AND IN-VITRO EVALUATION OF ITRACONAZOLE LOADED NANOSPONGES FOR T...Mahewash Sana Pathan
Itraconazole is an imidazole derivative and used for the treatment of local and systemic fungal infections. It is a BCS Class II drug having very low solubility in water i.e. 1-4ng/ml. The oral use of Itraconazole is not much recommended as it has many side effects. The present research has been undertaken with the aim to develop a topical hydrogel formulation of Itraconazole loaded nanosponges to increase the solubility, permeability and stability of itraconazole. Itraconazole loaded nanosponge was prepared by emulsion solvent diffusion method by using different concentrations of ethyl cellulose as a polymer, Polyvinyl alcohol as surfactant and dichloromethane as cross linking agent. Physical characteristics of the nanosponges as well as the drug entrapment efficiency, percentage drug content, Percent yield, drug polymer compatibility, solubility studies of the nanosponges were investigated. Particle size analysis and surface morphology of nanosponges were performed. The scanning electron microscopy of nanosponges showed that they were spherical in shape and spongy in nature. Drug entrapment efficiency was found to be in the range of 42.75 % to 73.10 %. The optimized nanosponge formulation was loaded into hydrogel using carbopol 940 and studied for pH, viscosity, in vitro drug release. Of the nanosponge formulations prepared, F4 was found to show drug release of 70.62%. It was concluded that Itraconazole nanosponge hydrogel may have increased solubility and drug release
Department of Pharmaceutical Management and Regulatory Affairs, Lachoo Memorial College of Science & Technology, Pharmacy Wing, Jodhpur National University, Rajasthan – 342003
Levetiracetam is an anti-epileptic medicine used to treat seizures in epilepsy. It is used for partial-onset, myoclonic, or tonic–clonic seizures. General route of administration is by Oral and IV. But it is not recommended throughout an epileptic attack due to the risk of nausea or vomiting. The Intranasal (IN) route is a promising therapy option, as it allows medications to reach the brain directly, it is regarded an alternative to parenteral administration, and hence the effective dosage predicted via other administration routes is scheduled to be reduced using the IN route
Investigation of linearity, detection limit (LD) and quantitation limit(LQ) of active substance from pharmaceutical tablets
The aim of this research was to exactly quantify pure sodium metamizole from tablets , using a
spectrophotometric analysis in Visible range. The method applied has been subjected to a validation protocol which consisted in analyzing the following parameters: linearity of the method, detection limit (LD) ,
quantitation limit (LQ). Following actual dosing, pure sodium metamizole amount in tablet of pharmaceutical was found to be
477.477 mg assigned to a percentage content of 95.495 %, very close to official declared amount (500 mg), with an maximum average percentage deviation of only 4.505 % from the official declared active substance content. This value was situated below the maximum admissible percentage deviation from stated active substance content (± 5%), established by Romanian Pharmacopoeia, X-th Edition rules.
Visible Spectrophotometric Analysis Method of Sodium Metamizole in Tablets
The aim of this research was to exactly quantify pure sodium metamizole from tablets , using a
spectrophotometric analysis in Visible range. The method applied has been subjected to a validation protocol which consisted in analyzing the following parameters: linearity of the method, detection limit (LD) ,
quantitation limit (LQ), Sandell’s sensitivity, interference of excipients, stability of prepared solutions, method and system precision, accuracy of the method. Following actual dosing, pure sodium metamizole amount
in tablet of pharmaceutical was found to be 477.477 mg assigned to a percentage content of 95.495 %, very close to official declared amount (500 mg), with an maximum average percentage deviation of only 4.505 % from the official declared active substance content. This value was situated below the maximum admissible percentage deviation from stated active substance content (± 5%), established by Romanian Pharmacopoeia, X-th Edition rules.
PREPARATION AND IN-VITRO EVALUATION OF ITRACONAZOLE LOADED NANOSPONGES FOR T...Mahewash Sana Pathan
Itraconazole is an imidazole derivative and used for the treatment of local and systemic fungal infections. It is a BCS Class II drug having very low solubility in water i.e. 1-4ng/ml. The oral use of Itraconazole is not much recommended as it has many side effects. The present research has been undertaken with the aim to develop a topical hydrogel formulation of Itraconazole loaded nanosponges to increase the solubility, permeability and stability of itraconazole. Itraconazole loaded nanosponge was prepared by emulsion solvent diffusion method by using different concentrations of ethyl cellulose as a polymer, Polyvinyl alcohol as surfactant and dichloromethane as cross linking agent. Physical characteristics of the nanosponges as well as the drug entrapment efficiency, percentage drug content, Percent yield, drug polymer compatibility, solubility studies of the nanosponges were investigated. Particle size analysis and surface morphology of nanosponges were performed. The scanning electron microscopy of nanosponges showed that they were spherical in shape and spongy in nature. Drug entrapment efficiency was found to be in the range of 42.75 % to 73.10 %. The optimized nanosponge formulation was loaded into hydrogel using carbopol 940 and studied for pH, viscosity, in vitro drug release. Of the nanosponge formulations prepared, F4 was found to show drug release of 70.62%. It was concluded that Itraconazole nanosponge hydrogel may have increased solubility and drug release
Department of Pharmaceutical Management and Regulatory Affairs, Lachoo Memorial College of Science & Technology, Pharmacy Wing, Jodhpur National University, Rajasthan – 342003
Levetiracetam is an anti-epileptic medicine used to treat seizures in epilepsy. It is used for partial-onset, myoclonic, or tonic–clonic seizures. General route of administration is by Oral and IV. But it is not recommended throughout an epileptic attack due to the risk of nausea or vomiting. The Intranasal (IN) route is a promising therapy option, as it allows medications to reach the brain directly, it is regarded an alternative to parenteral administration, and hence the effective dosage predicted via other administration routes is scheduled to be reduced using the IN route
Extractive Spectrophotometric Determination of Ulipristal Acetate using Napht...Ratnakaram Venkata Nadh
Ulipristal acetate is used to treat uterine fibroids and for emergency birth control. The present study is a first report on development of a visible spectrophotometric method for determination of Ulipristal acetate present in bulk and tablet formulation. The method involves the sequential addition of HCl (0.1 N) and Napthol Blue Black solution to Ulipristal acetate. Cation formed on tertiary amine group of Ulipristal acetate attracts anion of naphthol blue black (an acid dye) to develop a coloured ion-association complex. From the aqueous phase, the chromophore is extractable into chloroform, which exhibits λmax at 640 nm. As per the existing guidelines of ICH, various parameters of the method were tested for validation. Regression analysis (r > 0.999) shows that the plotted calibration curve exhibits good linearity in the studied range of concentration (2.50 – 15.00 μg mL-1). The % recovery values falls in 99.80 – 100.72 range. %RSD results of both precision studies were observed in the range 0.007 – 0.560, indicating the satisfactory precision of the method. Low values of R.S.D. (< 1 %) were observed indicating that the proposed method is reproducible, accurate and precise. The proposed method can be used in quality control laboratories for routine analysis of Ulipristal acetate (bulk drug and pharmaceutical dosage forms) without requirement of expensive instruments.
Extractive Spectrophotometric Determination of Ulipristal Acetate using Napht...Ratnakaram Venkata Nadh
Ulipristal acetate is used to treat uterine fibroids and for emergency birth control. The present study is a first report on development of a visible spectrophotometric method for determination of Ulipristal acetate present in bulk and tablet formulation. The method involves the sequential addition of HCl (0.1 N) and Napthol Blue Black solution to Ulipristal acetate. Cation formed on tertiary amine group of Ulipristal acetate attracts anion of naphthol blue black (an acid dye) to develop a coloured ion-association complex. From the aqueous phase, the chromophore is extractable into chloroform, which exhibits λmax at 640 nm. As per the existing guidelines of ICH, various parameters of the method were tested for validation. Regression analysis (r > 0.999) shows that the plotted calibration curve exhibits good linearity in the studied range of concentration (2.50 – 15.00 μg mL-1). The % recovery values falls in 99.80 – 100.72 range. %RSD results of both precision studies were observed in the range 0.007 – 0.560, indicating the satisfactory precision of the method. Low values of R.S.D. (< 1 %) were observed indicating that the proposed method is reproducible, accurate and precise. The proposed method can be used in quality control laboratories for routine analysis of Ulipristal acetate (bulk drug and pharmaceutical dosage forms) without requirement of expensive instruments.
Method Development and Validation of Naftopidil by Reverse Phase-HPLC in Bulk...SriramNagarajan15
A new simple, accurate, rapid and precise isocratic High performance liquid chromatographic (HPLC) method was developed and validated for the determination of Etomidate (ETO) injection. The Method employs Waters HPLC system on Develosil –ods-UG column (300 x 3.9 mm x 5µm) and flow rate of 1.5 mL/min with a load of 20 µL. Acetonitrile and Phosphate buffer was used as mobile phase in the composition of 40:60. The Detection was carried out at 254 nm. Linearity ranges for Etomidate was 40-240 µg/ml respectively. Retention Time of Etomidate was found to be 12.061 minutes respectively. Percent recovery study values of Etomidate were found to be within 98-102 %. This newly developed method was successfully utilized for the Quantitative estimation of Etomidate in injectables. This method was validated for accuracy, precision, linearity and Robustness as per ICH guidelines.
Method Development and Validation of Naftopidil by Reverse Phase-HPLC in Bulk...SriramNagarajan15
A new simple, accurate, rapid and precise isocratic High performance liquid chromatographic (HPLC) method was developed and validated for the determination of Etomidate (ETO) injection. The Method employs Waters HPLC system on Develosil –ods-UG column (300 x 3.9 mm x 5µm) and flow rate of 1.5 mL/min with a load of 20 µL. Acetonitrile and Phosphate buffer was used as mobile phase in the composition of 40:60. The Detection was carried out at 254 nm. Linearity ranges for Etomidate was 40-240 µg/ml respectively. Retention Time of Etomidate was found to be 12.061 minutes respectively. Percent recovery study values of Etomidate were found to be within 98-102 %. This newly developed method was successfully utilized for the Quantitative estimation of Etomidate in injectables. This method was validated for accuracy, precision, linearity and Robustness as per ICH guidelines.
Development and Validation of Analytical Methods for Simultaneous Spectrophot...ijtsrd
A simple, rapid UV Visible spectrophotometric method for the quantification of Pioglitazone hydrochloride and Glimepiride in bulk drug and tablet formulation was developed and validated. UV Visible spectrophotometric methods have been developed for the Derivative Spectrophotometric Method, of Pioglitazone and glimepiride in bulk and pharmaceutical dosage forms. the sampling wavelengths selected are 210 nm and 218 nm over the concentration ranges of 1.5 7.5 µg ml and 0.2 1.0 µg ml for pioglitazone and glimepiride respectively. Tejaswini Kande | Pallavi Dhekale | Supriya Khatal | Priyanka Borude "Development and Validation of Analytical Methods for Simultaneous Spectrophotometric Determination of Pioglitazone and Glimepiride by Derivative Method" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-4 | Issue-1 , December 2019, URL: https://www.ijtsrd.com/papers/ijtsrd29699.pdf Paper URL: https://www.ijtsrd.com/pharmacy/analytical-chemistry/29699/development-and-validation-of-analytical-methods-for-simultaneous-spectrophotometric-determination-of-pioglitazone-and-glimepiride-by-derivative-method/tejaswini-kande
Phytochemical composition and antiradical activity of Sakersia africana Hook....Open Access Research Paper
The valorization of the medicinal plants of our country and determination of their impact on health due to their abundance of substances with various pharmacological effects are our principal objective. This study was evaluated the phytochemical screening and radical 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) scavenging activity of different extracts of Sakersia africana Hook. f.. The results revealed that Sakersia africana Hook. f. is rich in phenols compounds, sterols, triterpenes, alkaloids and reducing compound. The values in total phenols and proanthocyanidines are ranging respectively from 391.58 ± 0.04 to 777 ± 0.03 mg/100 g of drugs and 113.5 ± 3.17 to 653.5 ± 36.83 mg/100 g of drugs. Results also show that different extracts tested present antiradical activity with values of IC50 ranging from 164.21± 0.014 to 195.54± 0.012 % and abundance in bioactive compounds. This study could justify the use of Sakersia africana of some chronic diseases.
A new precise accurate and reliable validated method for the determination of Capecitabine was developed by using
reverse phase high performance liquid chromatography in pharmaceutical dosage forms. Spectrophotometer
determination was carried out at an absorption maximum of 240nm by using methanol. The linearity was over the
concentration range of 20-120 μg/ml with correlation coefficient 0.999. Chromatographic separation was carried
out by using a mobile phase of methanol: Acetonitrile: water (80:20:80 V/V) on Waters 2487 dual absorbance
column in an isocratic mode at a flow rate of 1.1 ml/min with UV detection at 240 nm. The developed methods were
found to be precise and accurate for the estimation of Capecitabine in pharmaceutical dosage forms and could be
used for routine analysis.
Keywords: Capecitabine, RP-HPLC, Spectrophotometry, Waters 2487 dual absorbance detector, Nova pack 300 ×
3.9mm 5μ as column, 240nm
Design, synthesis, characterization and biological evaluation of 3- (4-(7-chl...iosrjce
Novel thiazolidinone derivatives TQ-VI(1-10) were designed, synthesized and screened for
antimicrobial activity. Synthesis of 3-(4-(7-chloro-2-(4-chlorophenyl) 4-oxo-quinazolin-3(4H)-yl) phenyl) -
2-arylthiazolidin-4-one TQ-VI(1-10) have been achieved from the starting material 2-amino-4-chlorobenzoic
acid TQ-I on cyclization with p-chlorobenzoyl chloride TQ-II to yield 7-chloro-2-(4-chlorophenyl)-4H-3,1-
benzoxazin-4-one,TQ-III, which on treatment with p-phenylindiamine gave 3-(4-aminophenyl)-7-chloro-2-(4-
chlorophenyl)quinazolin-4(3H)-one, TQ-IV in good yield. Then, TQ-IV on reaction with substituted aromatic
aldehydes converted to TQ-V(1-10), which on cyclization with thioglycolic acid gave TQ-VI (1-10). All the
synthesized compounds have been characterized on the basis of IR,
1
H-NMR and mass spectral data. The
compounds containing 4-OH, 4-OCH3 and 3,4,5-(OCH3)3 showed good activity. The title compounds were
screened for qualitative (zone of inhibition) and quantitative antimicrobial activity (MIC) by agar cup plate
method and serial dilution technique, respectively. Among the synthesized compounds in the series, the
compound TQVI4 and TQVI5 were found to exhibit significant antifungal activity at lower concentration of
31.25 µg/ml, against A.niger. The compound TQVI5 and TQVI4 showed zone of inhibition of 17mm and 15mm
against A.niger and C.albicans respectively which is comparable to that of standard drug. The rest of the
analogues in the series displayed weak to moderate antimicrobial activity when compared to the standard
positive controls Ampicillin and Amphotericin B.
Synthesis and Characterization of Schiff Base from Aromatic Amine and Aromati...ijtsrd
The synthesis of Schiff base From Aromatic Amine And Aromatic P Nitro benzaldehyde was performed by a novel method of stirring followed by the addition of p nitro benzaldehydeandm nitro aniline 0.02M . Characterization of the synthesized compounds, determination of purity and identity of the compounds using following spectroscopic and chromatographic techniques Solubility, Thin Layer Chromatographic studies, Ultra Violet studied rotational and vibrational studies FT IR studies. The compounds were investigated for their Antimicrobial activity by cup plate method. Compound1 nitro 4 1 imino,4 nitrophenyl benzene was found to be the most active according to pharmacological evaluation exhibited antimicrobial. Ms. Chetana D. Patil | Mr. Digamber N. Bhosale | Ms. Smita P. Bedis "Synthesis and Characterization of Schiff Base from Aromatic Amine and Aromatic P-Nitro Benzaldehyde" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-5 , August 2019, URL: https://www.ijtsrd.com/papers/ijtsrd26401.pdfPaper URL: https://www.ijtsrd.com/pharmacy/medicinal-chemistry/26401/synthesis-and-characterization-of-schiff-base-from-aromatic-amine-and-aromatic-p-nitro-benzaldehyde/ms-chetana-d-patil
Iodometric Quantitative Analysis Method of Ascorbic Acid in Tablets
Ascorbic acid is a water-soluble vitamin provided with strong antioxidant action, that fulfills an important immune protective role of the body against infections and prevents various cancers appearance. The main goal of this study was to exactly quantify pure ascorbic acid in tablets of two pharmaceuticals. Proposed
objective consisted in improvement and application of a iodometric titration method in ascorbic acid quantitative analysis. Ascorbic acid content per tablet in both studied pharmaceuticals was 173.84 mg, very close to official stated amount of active substance (180 mg). Allowed percentage deviation from declared content of pure ascorbic acid was only 3.42 %, below maximum value of ± 5 % imposed by Romanian Pharmacopoeia 10-th Edition, according to European and International standards. Statistical analysis confirmed experimental obtained results and revealed low Standard Error value SE = 0.214476, which has fallen within normal limits. Confidence Level value (95.0 %) = 0.551328 and Standard Deviation SD = 0.525357. were within normal range of values. Relative Standard Deviation (Coefficient of variation or homogeneity) RSD = 26.268% was found below maximum range of accepted values (30-35%). P value = 7.44. 10-6 was located within normal limits, P < 0.001, so the experimental obtained results has shown highest statistical significance. Thus, studied titration method can be successfully used in quantitative
analysis of ascorbic acid from different samples.
BIOLOGICAL ACTIVITY OF THE OXIDIZED POLYSACCHARIDES
Bio-reactive polysaccharides have been most commonly used as drugs or drug delivery systems. The present paper describes the biological activity of some artificial and natural polyanionic polysaccharides.
Results regarding oxidized cellulose and carboxymethylcellulose
modified with benzocaine or N – hydroxy – 3,4-dihydroxybenzamide
(Didox) complete the picture of antiviral and antitumoral effects of polysaccharides. The biological tests regarding antiviral and antitumor activity showed that the introduction of benzocaine as a spacer unit between the main chain and a CMC carboxylic group enhances the antiviral and antitumor activity of carboxymethylcellulose.
Extractive Spectrophotometric Determination of Ulipristal Acetate using Napht...Ratnakaram Venkata Nadh
Ulipristal acetate is used to treat uterine fibroids and for emergency birth control. The present study is a first report on development of a visible spectrophotometric method for determination of Ulipristal acetate present in bulk and tablet formulation. The method involves the sequential addition of HCl (0.1 N) and Napthol Blue Black solution to Ulipristal acetate. Cation formed on tertiary amine group of Ulipristal acetate attracts anion of naphthol blue black (an acid dye) to develop a coloured ion-association complex. From the aqueous phase, the chromophore is extractable into chloroform, which exhibits λmax at 640 nm. As per the existing guidelines of ICH, various parameters of the method were tested for validation. Regression analysis (r > 0.999) shows that the plotted calibration curve exhibits good linearity in the studied range of concentration (2.50 – 15.00 μg mL-1). The % recovery values falls in 99.80 – 100.72 range. %RSD results of both precision studies were observed in the range 0.007 – 0.560, indicating the satisfactory precision of the method. Low values of R.S.D. (< 1 %) were observed indicating that the proposed method is reproducible, accurate and precise. The proposed method can be used in quality control laboratories for routine analysis of Ulipristal acetate (bulk drug and pharmaceutical dosage forms) without requirement of expensive instruments.
Extractive Spectrophotometric Determination of Ulipristal Acetate using Napht...Ratnakaram Venkata Nadh
Ulipristal acetate is used to treat uterine fibroids and for emergency birth control. The present study is a first report on development of a visible spectrophotometric method for determination of Ulipristal acetate present in bulk and tablet formulation. The method involves the sequential addition of HCl (0.1 N) and Napthol Blue Black solution to Ulipristal acetate. Cation formed on tertiary amine group of Ulipristal acetate attracts anion of naphthol blue black (an acid dye) to develop a coloured ion-association complex. From the aqueous phase, the chromophore is extractable into chloroform, which exhibits λmax at 640 nm. As per the existing guidelines of ICH, various parameters of the method were tested for validation. Regression analysis (r > 0.999) shows that the plotted calibration curve exhibits good linearity in the studied range of concentration (2.50 – 15.00 μg mL-1). The % recovery values falls in 99.80 – 100.72 range. %RSD results of both precision studies were observed in the range 0.007 – 0.560, indicating the satisfactory precision of the method. Low values of R.S.D. (< 1 %) were observed indicating that the proposed method is reproducible, accurate and precise. The proposed method can be used in quality control laboratories for routine analysis of Ulipristal acetate (bulk drug and pharmaceutical dosage forms) without requirement of expensive instruments.
Method Development and Validation of Naftopidil by Reverse Phase-HPLC in Bulk...SriramNagarajan15
A new simple, accurate, rapid and precise isocratic High performance liquid chromatographic (HPLC) method was developed and validated for the determination of Etomidate (ETO) injection. The Method employs Waters HPLC system on Develosil –ods-UG column (300 x 3.9 mm x 5µm) and flow rate of 1.5 mL/min with a load of 20 µL. Acetonitrile and Phosphate buffer was used as mobile phase in the composition of 40:60. The Detection was carried out at 254 nm. Linearity ranges for Etomidate was 40-240 µg/ml respectively. Retention Time of Etomidate was found to be 12.061 minutes respectively. Percent recovery study values of Etomidate were found to be within 98-102 %. This newly developed method was successfully utilized for the Quantitative estimation of Etomidate in injectables. This method was validated for accuracy, precision, linearity and Robustness as per ICH guidelines.
Method Development and Validation of Naftopidil by Reverse Phase-HPLC in Bulk...SriramNagarajan15
A new simple, accurate, rapid and precise isocratic High performance liquid chromatographic (HPLC) method was developed and validated for the determination of Etomidate (ETO) injection. The Method employs Waters HPLC system on Develosil –ods-UG column (300 x 3.9 mm x 5µm) and flow rate of 1.5 mL/min with a load of 20 µL. Acetonitrile and Phosphate buffer was used as mobile phase in the composition of 40:60. The Detection was carried out at 254 nm. Linearity ranges for Etomidate was 40-240 µg/ml respectively. Retention Time of Etomidate was found to be 12.061 minutes respectively. Percent recovery study values of Etomidate were found to be within 98-102 %. This newly developed method was successfully utilized for the Quantitative estimation of Etomidate in injectables. This method was validated for accuracy, precision, linearity and Robustness as per ICH guidelines.
Development and Validation of Analytical Methods for Simultaneous Spectrophot...ijtsrd
A simple, rapid UV Visible spectrophotometric method for the quantification of Pioglitazone hydrochloride and Glimepiride in bulk drug and tablet formulation was developed and validated. UV Visible spectrophotometric methods have been developed for the Derivative Spectrophotometric Method, of Pioglitazone and glimepiride in bulk and pharmaceutical dosage forms. the sampling wavelengths selected are 210 nm and 218 nm over the concentration ranges of 1.5 7.5 µg ml and 0.2 1.0 µg ml for pioglitazone and glimepiride respectively. Tejaswini Kande | Pallavi Dhekale | Supriya Khatal | Priyanka Borude "Development and Validation of Analytical Methods for Simultaneous Spectrophotometric Determination of Pioglitazone and Glimepiride by Derivative Method" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-4 | Issue-1 , December 2019, URL: https://www.ijtsrd.com/papers/ijtsrd29699.pdf Paper URL: https://www.ijtsrd.com/pharmacy/analytical-chemistry/29699/development-and-validation-of-analytical-methods-for-simultaneous-spectrophotometric-determination-of-pioglitazone-and-glimepiride-by-derivative-method/tejaswini-kande
Phytochemical composition and antiradical activity of Sakersia africana Hook....Open Access Research Paper
The valorization of the medicinal plants of our country and determination of their impact on health due to their abundance of substances with various pharmacological effects are our principal objective. This study was evaluated the phytochemical screening and radical 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) scavenging activity of different extracts of Sakersia africana Hook. f.. The results revealed that Sakersia africana Hook. f. is rich in phenols compounds, sterols, triterpenes, alkaloids and reducing compound. The values in total phenols and proanthocyanidines are ranging respectively from 391.58 ± 0.04 to 777 ± 0.03 mg/100 g of drugs and 113.5 ± 3.17 to 653.5 ± 36.83 mg/100 g of drugs. Results also show that different extracts tested present antiradical activity with values of IC50 ranging from 164.21± 0.014 to 195.54± 0.012 % and abundance in bioactive compounds. This study could justify the use of Sakersia africana of some chronic diseases.
A new precise accurate and reliable validated method for the determination of Capecitabine was developed by using
reverse phase high performance liquid chromatography in pharmaceutical dosage forms. Spectrophotometer
determination was carried out at an absorption maximum of 240nm by using methanol. The linearity was over the
concentration range of 20-120 μg/ml with correlation coefficient 0.999. Chromatographic separation was carried
out by using a mobile phase of methanol: Acetonitrile: water (80:20:80 V/V) on Waters 2487 dual absorbance
column in an isocratic mode at a flow rate of 1.1 ml/min with UV detection at 240 nm. The developed methods were
found to be precise and accurate for the estimation of Capecitabine in pharmaceutical dosage forms and could be
used for routine analysis.
Keywords: Capecitabine, RP-HPLC, Spectrophotometry, Waters 2487 dual absorbance detector, Nova pack 300 ×
3.9mm 5μ as column, 240nm
Design, synthesis, characterization and biological evaluation of 3- (4-(7-chl...iosrjce
Novel thiazolidinone derivatives TQ-VI(1-10) were designed, synthesized and screened for
antimicrobial activity. Synthesis of 3-(4-(7-chloro-2-(4-chlorophenyl) 4-oxo-quinazolin-3(4H)-yl) phenyl) -
2-arylthiazolidin-4-one TQ-VI(1-10) have been achieved from the starting material 2-amino-4-chlorobenzoic
acid TQ-I on cyclization with p-chlorobenzoyl chloride TQ-II to yield 7-chloro-2-(4-chlorophenyl)-4H-3,1-
benzoxazin-4-one,TQ-III, which on treatment with p-phenylindiamine gave 3-(4-aminophenyl)-7-chloro-2-(4-
chlorophenyl)quinazolin-4(3H)-one, TQ-IV in good yield. Then, TQ-IV on reaction with substituted aromatic
aldehydes converted to TQ-V(1-10), which on cyclization with thioglycolic acid gave TQ-VI (1-10). All the
synthesized compounds have been characterized on the basis of IR,
1
H-NMR and mass spectral data. The
compounds containing 4-OH, 4-OCH3 and 3,4,5-(OCH3)3 showed good activity. The title compounds were
screened for qualitative (zone of inhibition) and quantitative antimicrobial activity (MIC) by agar cup plate
method and serial dilution technique, respectively. Among the synthesized compounds in the series, the
compound TQVI4 and TQVI5 were found to exhibit significant antifungal activity at lower concentration of
31.25 µg/ml, against A.niger. The compound TQVI5 and TQVI4 showed zone of inhibition of 17mm and 15mm
against A.niger and C.albicans respectively which is comparable to that of standard drug. The rest of the
analogues in the series displayed weak to moderate antimicrobial activity when compared to the standard
positive controls Ampicillin and Amphotericin B.
Synthesis and Characterization of Schiff Base from Aromatic Amine and Aromati...ijtsrd
The synthesis of Schiff base From Aromatic Amine And Aromatic P Nitro benzaldehyde was performed by a novel method of stirring followed by the addition of p nitro benzaldehydeandm nitro aniline 0.02M . Characterization of the synthesized compounds, determination of purity and identity of the compounds using following spectroscopic and chromatographic techniques Solubility, Thin Layer Chromatographic studies, Ultra Violet studied rotational and vibrational studies FT IR studies. The compounds were investigated for their Antimicrobial activity by cup plate method. Compound1 nitro 4 1 imino,4 nitrophenyl benzene was found to be the most active according to pharmacological evaluation exhibited antimicrobial. Ms. Chetana D. Patil | Mr. Digamber N. Bhosale | Ms. Smita P. Bedis "Synthesis and Characterization of Schiff Base from Aromatic Amine and Aromatic P-Nitro Benzaldehyde" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-5 , August 2019, URL: https://www.ijtsrd.com/papers/ijtsrd26401.pdfPaper URL: https://www.ijtsrd.com/pharmacy/medicinal-chemistry/26401/synthesis-and-characterization-of-schiff-base-from-aromatic-amine-and-aromatic-p-nitro-benzaldehyde/ms-chetana-d-patil
Iodometric Quantitative Analysis Method of Ascorbic Acid in Tablets
Ascorbic acid is a water-soluble vitamin provided with strong antioxidant action, that fulfills an important immune protective role of the body against infections and prevents various cancers appearance. The main goal of this study was to exactly quantify pure ascorbic acid in tablets of two pharmaceuticals. Proposed
objective consisted in improvement and application of a iodometric titration method in ascorbic acid quantitative analysis. Ascorbic acid content per tablet in both studied pharmaceuticals was 173.84 mg, very close to official stated amount of active substance (180 mg). Allowed percentage deviation from declared content of pure ascorbic acid was only 3.42 %, below maximum value of ± 5 % imposed by Romanian Pharmacopoeia 10-th Edition, according to European and International standards. Statistical analysis confirmed experimental obtained results and revealed low Standard Error value SE = 0.214476, which has fallen within normal limits. Confidence Level value (95.0 %) = 0.551328 and Standard Deviation SD = 0.525357. were within normal range of values. Relative Standard Deviation (Coefficient of variation or homogeneity) RSD = 26.268% was found below maximum range of accepted values (30-35%). P value = 7.44. 10-6 was located within normal limits, P < 0.001, so the experimental obtained results has shown highest statistical significance. Thus, studied titration method can be successfully used in quantitative
analysis of ascorbic acid from different samples.
BIOLOGICAL ACTIVITY OF THE OXIDIZED POLYSACCHARIDES
Bio-reactive polysaccharides have been most commonly used as drugs or drug delivery systems. The present paper describes the biological activity of some artificial and natural polyanionic polysaccharides.
Results regarding oxidized cellulose and carboxymethylcellulose
modified with benzocaine or N – hydroxy – 3,4-dihydroxybenzamide
(Didox) complete the picture of antiviral and antitumoral effects of polysaccharides. The biological tests regarding antiviral and antitumor activity showed that the introduction of benzocaine as a spacer unit between the main chain and a CMC carboxylic group enhances the antiviral and antitumor activity of carboxymethylcellulose.
The keyboard remains the least ergonomically designed computer device
Ergonomic devices are often designed to provide more comfort and to increase productivity but they can also help avoid pain and specific injuries. The ergonomic design of a computer keyboard needs expertise in ergonomics and biomechanics. The existence of a large category of typists with slow typing skills, visually searching the seemingly random keyboard, including novice users and the analysis of existing standards and keyboards leads to the conclusion that existing QWERTY based keyboards still remain the least ergonomically designed computer devices and need to be improved. This paper discusses the existing standards in ergonomics and the various commercial keyboards and makes observations about the ergonomic design features and the wrong recommendations of some standards.
Particularities of Statin Therapy in Diabetic Patients
The purpose of conducted study was to determine which of the different types of statins ensure a better control of biological markers in diabetic patients and which of the lipid molecules studied, could be a first choice of lipid-lowering therapy in people suffering from diabetes. The measurement of blood glucose
levels depending on type of statin used, was another target of this research. Dyslipidemia was a major risk factor for cardiovascular complications in people with diabetes. It was found that atorvastatin was the most
effective statin in controlling dyslipidemia in diabetic, because has ensured optimum control of HDL, LDL - cholesterol, triglycerides and glycemic values, effect which was resulted from the particular chemical structure of atorvastatin. Atorvastatin was discovered to be the best predictor in diabetes mellitus type 2 treatment, with a sensitivity about 78% and specificity to 45%., the most highest values compared to Rosuvastatin and Simvastatin. Area Under the Curve (AUC = 0.610; AUC >0.600)
Pharmacological Principles Used in Patient Monitoring with Type 2 Diabetes
This study assessed the medication used in type 2 diabetes treatment, depending on the glycaemia level and set out the oral anti-diabetics which are recommended, in three study stages: admission, hospitalization
and discharge. Eighty patients were selected and diagnosed with diabetes mellitus 2 type, who were registered in the diabetes and nutrition diseases department within Sf. Apostol Andrei Galati Hospital. They
were subjected to a series of laboratory tests: blood count, glycosylated haemoglobin, glycaemia level. It were established main classes of anti-diabetic drugs outpatient used and the main types of anti diabetic agents administrated to patients requiring hospitalization, compared to high glycaemia values. It was given also, the medication used to normalize blood glucose levels during hospitalization and also at discharge. The biguanides associated with sulphonylureas drugs did not provide an adequate glycaemia control, so insulin must be combined with Metformin to normalize blood glucose levels as soon as possible. Glycaemia control was improved and the hypoglycemia risk was reduced regarding obese patient undergoing treatment
with insulin, to whom biguanides were administered.
Photochemical Stability of a Cotton Fabric Surface Dyed with a Reactive TriphenodioxazIne Dye
The paper describes the photochemical stability of a commercial triphenodioxazine dye (Reactive Blue_204) linked onto a cotton fabric. Preliminary studies have shown that as a result of irradiation, the dye and its photodegradation products can pass directly onto the skin under conditions that mimic human perspiration and cause side-effects. The cotton dyed fabric was photo irradiated at different time intervals. Standard methods were employed to evaluate the color strength
at various levels of pH, temperature, dyeing contact time, and salt concentration. The influence of UV radiation at different doses ( > 300 nm) on the structural and color modifications of the dyed cotton
fabrics was studied. Structural modifications before and after irradiation were compared by applying FTIR, UV–Vis, and near infrared chemical imaging (NIR–CI) techniques. Color modifications were investigated with the CIELAB system. Color differences significantly increased with the irradiation dose. High irradiation doses caused changes in the dye structure.
A RETROSPECTIVE STUDY ABOUT INSTITUTIONALIZED ELDERLY LIFE CONDITIONS IN THREE SOCIAL CARE CENTERS
A retrospective study was made on 195 institutionalized elderly people and was carried out between 2002-2006 in three social care centers from Galati county: “Sf. Spiridon” retirement home (A), “Stefan cel Mare” social care center (B) and Medicalsocial center (C). The aim of this study is to find out the real social life conditions of institutionalized elderly, the shortcomings and positive aspects of their existence, in order to design future measures to improve quality of elderly life in all aspects. Actual quality of life was assessed by, taking into account physical and psychosocial environment,
quality of elderly social care, satisfaction degree of assisted persons in relation to living
conditions and existing relationships, their health state, as well as to identify the causes
that led institutionalization of such persons As working tools were used: individual questionnaire, training manual, sampling lists, summary tables. The study undertaken in Romania confirmed that elderly coming from “Sf. Spiridon “retirement home have the highest average age (up to 75 years old). Loneliness was the main reason which caused elderly admission for institutionalization in three nursing homes (49.6% among the elderly in the Sf. Spiridon retirement home (A), 43.2% for those from “Stefan cel Mare” social care center (B) and 38.1% of the elderly from Medical-social center (C). Other important reasons were represented by lack of housing and insufficient storage house space. The highest elderly percentage who came into social care centers A, B, C on their self will is 85.3%, 64.9% and 45.2%, respectively.
Photochemical stability of cellulose textile surfaces painted with some reactive azo-triazine dyes
The influence of ultraviolet irradiation of different doses (k[300 nm) on the structural and color modifications of cotton fabrics painted with four different
azo-triazine dyes (Reactive Yellow 143, Reactive Orange 13, Reactive Red 183, and Reactive Red 2) was studied. High irradiation doses up to 3500 J cm-2 led to changes in
the dyes structures. Structural changes before and after the
complete irradiation were compared by applying FTIR,
UV–Vis, and near infrared chemical imaging techniques.
Color modifications were also investigated. Color differences significantly increased with the irradiation dose for all the studied samples.
DIETARY SURVEY IN AN OBESE POPULATION
The body mass index (BMI) shows a rate of 17.4% patients suffering from first degree fatness and 56.5% from second degree fatness. The frequency of the illness increases with age, the differences established being statistically important for a p of O. 001. 45. 6 % persons were diagnosed with HTA, with differences statiscally important at a p of 0.01, so the number of cases increases with age. The same situation is to be found in cardiovascular illnesses (angina pectoris, ischaemic cardiopathy). The high level of cholesterol is more frequent at older people, and still the differences established are statistically not important. In these conditions, the food diet must be adapted to the patient's condition. A comparative study was made on groups of age, using the following groups of food: cereal derivatives, vegetables, fruits, dairy produce, meat, fish. In most of the cases, the differences established are statistically non important. So, even if the diagnosis is known, the patient doesn't give up his feeding habits.
SPECTROSCOPY ANALYSIS OF REACTIVE RED 2 DYE
Reactive Red 2 is a reactive red dye used for textile painting and printing. Before using in chemical laboratory analysis, this reactive dye must be evaluated through validation methods, which consists of: linearity, specificity, precision, accuracy and stability of solutions. The aim of this study is to present co-linearity method, which is a little part of validation methods. It is graphical represented the dependence of absorbances obtained through spectroscopy analysis in visible domain (544 nm), under some values of concentrations. Statistical parameters and the concentrations domain, for which the method presents linearity, will be calculated.
SPECTROSCOPY ANALYSIS METHOD OF TWO REACTIVE DYES USED IN TEXTILE INDUSTRY
Reactive Blue 204 and Reactive Red 183 are two reactive dyes
frequently used for textile painting and printing. Before using in chemical laboratory analysis,
these reactive dyes must be evaluated through validation methods which consists of linearity, stability of solutions, specificity, precision and accuracy. The purpose of this study is to present the validation process of these two reactive dyes. First of all it is graphical represented the dependence of absorbances obtained through spectroscopy analysis in visible domain (636 nm and 503 nm), under some values of concentrations. Statistical parameters and the concentration
domains for which the method presents linearity will be calculated. After that, stability of
solutions, specificity, precision and accuracy including some statistical parameters, will accomplish the validation of process.
RESEARCHES ON IMMUNE SYSTEM RESPONSE, SKIN AND HEPATIC REACTIVITY IN LABORATORY ANIMALS AFTER RETRO-AURICULAR TOPICAL ADMINISTRATION OF TWO REACTIVE TEXTILE DYES
Ergonomic devices are often designed to provide more comfort and to increase productivity but they can also help avoid pain and specific injuries. The ergonomic design of a computer keyboard needs expertise in ergonomics and biomechanics. The existence of a large category of typists with slow typing skills, visually searching the seemingly random keyboard, including novice users, and the analysis of existing standards and keyboards leads to the conclusion that existing QWERTY-based keyboards still remain the least ergonomically designed computer devices and need to be improved. This paper discusses the existing standards in ergonomics and the various commercial keyboards and makes observations about the ergonomic design features and the wrong recommendations of some standards.
Reactive dyes are synthetic organic compounds used on a wide scale in the textile industry, for painting materials of different types and compositions (e.g. 100% cotton, wool, natural satin, viscose, synthetic fibers). Reactive dyes are solid compounds (powders) completely water-soluble at normal temperature and pressure conditions. Their structures contain chromophore groups, which generate color, and autochrome groups, which determine the compound's water solubility and the capacity to fix the textile fiber. Such organic compounds absorb UV-Vis radiations at specific wavelengths, corresponding to maximum absorption peaks, in both solution and dyed fiber. The human organism, through the dyed clothing, comes in direct contact with those dyes which can undergo modifications once exposed to UV radiations, having the possibility to reach the organism via transdermal transport. As it is known, the provoked negative effects are stronger during summer when UV radiations are more intense and in order to reduce their intensity dark-colored clothing is avoided. Dyes can be transformed into compounds that are easily absorbed into the skin. Some of these metabolites can be less toxic than the original corresponding dye, whilst others, such as free radicals, are potentially cancerous. Knowledge of the biological effects of the organic dyes, reactive dyes in particular, correlated with their structural and physical characteristics, permanently consists an issue of high scientific and practical interest and its solution may contribute to the diminishing of risk factors and improving population health. UV radiation's influence on the structural and color modifications of textile materials was studied. Color modifications are due to structural changes in aromatic and carbonyl groups. In most cases, photo-oxidative processes were identified in the dye structure. Dyeing was performed using non-irradiated and irradiated cotton painted with reactive blue dye 204.
Textile dyes have been reported as causing various stages of contact dermatitis. Reactive dyes are widely applied in dyeing cellulose fiber-based textiles (100% cotton), skin fibers (hemp, flax), regenerated cellulose (cellulose acetate, viscose), protein fibers (natural silk, wool). The human body comes in contact daily with such compounds. This aspect is important for elucidating their biological effects on the human body, in correlation with Physico-chemical properties. Dyes are chemical compounds containing chromophore and autochrome groups. The authors herein report results concerning the influence of UV irradiation with λ > 300 nm on the structure and properties of different colored textiles. Subjects to study were textiles painted with four azo-triazine-based dyes which were exposed to 100 h UV irradiation time and irradiation dose values up to 3500 J cm-2. The five azo dyes were: reactive orange 13, reactive red 183, reactive yellow 143, reactive blue 204, and reactive red 2. Structural modifications as a result of irradiation were undertaken by UV-Vis spectroscopy. It was observed that during UV exposure there occurred partial dyes detachment from the textiles, accompanied by glucosidic units and dye photodecomposition by C–N bond scission and degradation of aromatic entities and azo-based chromophores. Color modifications were also investigated. Color differences significantly increased with the irradiation dose for all the studied samples.
Chimie Anorganica - Curs 2, Sef Lucr. Dr. Farm. Gavat Cristian-Catalin, Facultatea de Bioinginerie Medicala, Departamentul de Stiinte Biomedicale, Universitatea de Medicina si Farmacie GRIGORE T. POPA, Iasi, Str. Universitatii nr. 16, Cod Postal 700115, Romania
Chimie Anorganica - Curs 1 , Sef Lucr. Dr. Farm. Gavat Cristian-Catalin, Facultatea de Bioinginerie Medicala, Departamentul de Stiinte Biomedicale, Universitatea de Medicina si Farmacie GRIGORE T. POPA, Iasi, Str Universitatii nr. 16, Cod Postal 700115, Romania
More from Grigore T. Popa University of Medicine and Pharmacy, 16 Universitatii Street, Iasi, 700115, Romania (19)
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...University of Maribor
Slides from:
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Track: Artificial Intelligence
https://www.etran.rs/2024/en/home-english/
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
2. solution, 150 µg/mL (0.015%). The maximum absorption
wavelength was found to be at λ max. = 458 nm (Fig. 1).
Fig. 1 Absorption spectrum of a bright orange dye with a yellowish shade
obtained from ibuprofen
Specific absorptivity or specific extinction represented the
absorbance (as a measure of the absorption of the selected
electromagnetic radiation) of a solution layer with a thickness
of 1 cm and a concentration of 1% (g / 100 mL) It was
calculated and the result obtained was shown below.
Analysis method description. Ibuprofen, 2- (4-isobutyl-
phenyl) propanoic acid, a non-steroidal anti-inflammatory drug
derived from propionic acid, present as an active substance in a
pharmaceutical product called Ibuprofen Grindex®
, reacted
completely with an α-naphthylamine 0.04 % solution, in the
presence of sodium nitrite NaNO2 4% aqueous solution, by
heating 5 minutes at 50-60°C, followed by a forced cooling for
10 minutes on an ice bath, that led to the quantitative
production of a bright orange colored compound with a
yellowish shade (an azo-dye), which was obtained in an
amount perfectly equivalent to Ibuprofen present in the studied
sample. By spectrophotometric analysis of the obtained azo-
dye at the wavelength corresponding to its absorption
maximum λ max. = 458 nm, Ibuprofen in the analyzed, sample
was directly spectrophotometrically measured, in relation to the
absolute ethanol p.a. as a control (Fig. 2).
Fig. 2. Chemical reaction pathway of Ibuprofen assigned to the synthesis of a
bright orange dye with a yellowish shade
The absorbances of ten standard solutions (0.75 µg-mL –
15.00 µg/mL) were measured. These solutions have been
prepared directly from the standard working solution 150
µg/mL according to Table I. Final solutions volume in each
graduated test tube was 20 mL, after making up to the mark
with absolute ethanol (TABLE I).
TABLE I. Obtaining the set of standard ibuprofen solutions from standard
working solution
mL Ibuprofen
standard working
solution,
150 µg/mL
Required reagents added to standard solutions
mL alpha-
naphthylamine,
0.04 %
mL sodium
nitrite, NaNO2, 4
%
mL absolute
ethanol
0.1 0.5 0.3 19.1
0.2 0.5 0.3 19.0
0.3 0.5 0.3 18.9
0.4 0.5 0.3 18.8
0.6 0.5 0.3 18.6
0.8 0.5 0.3 18.4
1.0 0.5 0.3 18.2
1.2 0.5 0.3 18.0
1.6 0.5 0.3 17.6
2.0 0.5 0.3 17.2
B.1. Ibuprofen sample preparation and calculation procedure.
Average weight of a pharmaceutical tablet was mc =
0.5661 g = 566.1 mg. The official active substance content of
pure ibuprofen on the pharmaceutical tablet was 400 mg. a =
0.0516 g of ibuprofen powder and passed with 10 mL of
absolute ethyl alcohol in a Berzelius beaker. The obtained
solution was transferred into a volumetric flask of volume V =
100 mL. A volume of v = 0.3 mL was measured, into a
graduated test tube of volume VP = 20 mL and α-
naphthylamine solution 0.04% a NaNO2, 4% were added (table
II). It was heated on a water bath to 50-60 ⁰C at constant
temperature for 5 minutes. The sample solution was kept on an
ice bath for 10 minutes and then filled with absolute ethanol to
the mark. (TABLE II). Mean sample absorbance was Ap =
0.273.
TABLE II. PREPARATION OF THE SAMPLE IBUPROFEN SOLUTION
Sample
solution of
Ibuprofen
Grindex®
(mL)
Reagents added to the sample solution
mL alpha-
naphthylamine,
0.04 % solution
mL sodium
nitrite,
NaNO2, 4 %
aqueous
mL absolute
ethanol
3. solution
0.3 0.5 0.3 18.9
B.2. Statistic analysis.. The aim was to determine the
following parameters: the linearity of the proposed method,
detection limit (LD), quantitation limit (LQ), stability of the
standard solutions, and system precision that consisted of the
standard solutions containing pure Ibuprofen and the UV-VIS
spectrophotometer utilized for measurements, as a whole.
Linearity of the proposed method. The linearity of an
analysis process consisted of the ability to lead to results
directly proportional to the concentration of an analyte in a
given sample, within a given range (0.75 μg/mL-15.00
μg/mL). The correlation coefficient had to be R > 0.999 and
linear regression coefficient R2
≥ 0.999. Microsoft Office
Excel 2016 software was used (TABLE III).
TABLE III. STATISTICAL PARAMETERS OF THE LINEARITY
Observations
(measured
absorbance
values)
Regression Statistics
Correlation
coefficient
(Multiple
R)
Linear
regression
coefficient
R square,
R2)
Adjusted R
square
(adjusted
R2)
Standard
error of the
linear
regression
(SE)
10 0.999676 0.999352 0.999271 0.004974
Detection limit (LD) and quantitation limit (LQ),
Detection limit (LD) was the smallest amount of analyte that
could be detected in a sample compared to a blank. It was
evaluated using the formula: LD = 3. SE/ slope (A). SE has
represented standard error of the regression line. The
Quantitation limit (LQ) was given by the lowest analyte
concentration in a sample, which could be quantified. Its value
was calculated as follows: LQ = 10. SE / slope (B).
Stability of the prepared standard solutions A standard
solution with a concentration of 5.0 μg/mL was chosen from
the set of ten standard solutions prepared (from Table I). This
solution was investigated for 32 hours at λ = 458 nm from the
time of its preparation, at various intervals, in normal storage
conditions. Relative standard deviation (coefficient of
variation) RSD %: RSD % = (SD. 100)/ X Average (C). For
accurate measurements, RSD ≤ 5%. (TABLE IV).
TABLE IV. STABILITY ANALYSIS OF IBUPROFEN STANDARD SOLUTIONS
Number of
experimental
trials
Stability over time of the standard solutions containing
pure Ibuprofen
Time
(measured
in hours)
Mean
measured
absorbance
A(λ)
The
mean
value
Standard
deviation
(SD)
Relative
standard
deviation
(RSD %)
1. 0 0.271
0.2671 0.003871 1.4493
2. 2 0.270
3. 4 0.270
4. 6 0.268
5. 12 0.269
6. 18 0.266
7. 24 0.263
8. 32 0.260
System Precision. For system precision investigation, a
target standard solution of 1.50 μg./ mL was chosen from the
standard solutions set (from Table I) and processed under
established experimental conditions. Standard deviation (SD)
and RSD% were calculated. RSD ≤ 5% (TABLE V).
TABLE V. SYSTEM PRECISION ANALYSIS
Number of
experimental
trials
System precision, as a whole, that consists of the standard
solutions containing pure Ibuprofen and the UV-VIS
spectrophotometer
Standard
solution
of
Ibuprofen,
1.50
μg/mL
Mean
measured
absorbance
A(λ)
The
mean
value
Standard
deviation
(SD)
Relative
standard
deviation
(RSD %)
1. 0.271
0.1146 0.003050 2.6614
2. 0.270
3. 0.270
4. 0.268
5. 0.269
III. RESULTS
a. Calculation of Ibuprofen concentration Cp (μg/mL)
taken into study, from the calibration graph (Fig. 3).
Fig. 3 Calibration graph obtained for Ibuprofen standard solutions (0.75
μg/mL -15.00 μg/mL)
From the regression line y = 0.039 x + 0.0608 (Fig, 3), y =
Ap = 0.273 and x = Cp (μg/mL). Thus, Cp (μg/mL), = (0.273 –
0.0608)/0.039 (1). Cp = 5.441 μg/mL pure Ibuprofen. Ap =
0.273 = mean absorbance of the sample solution.
b. Calculation of Ibuprofen pure content (X µg) from VP
= 20 mL solution existing in the graduated test tube,
depending on CP (µg/mL) found above: CP µg →1 mL
solution, so in VP = 20 mL solution were X = 20. CP µg of
Pure Ibuprofen (2). X = 20. 5.441. So, X = 108.82 µg of
Ibuprofen.
c. Ibuprofen pure content estimation (Y µg) from the
initial solution existing in V = 100 mL volumetric flask,
depending on X (µg) value: If X µg Ibuprofen → v = 0.3 mL
sample solution, then in V = 100 mL volumetric flask were Y
= (V. X) / v µg pure Ibuprofen (3). Y = (100. 108.82) / 0.3.
Thus, Y = 36273.33 µg pure Ibuprofen
4. d. Ibuprofen pure content evaluation (Y1 µg transformed
in mg), calculated on a pharmaceutical tablet, as a final
result, reported to mc = 0.5661 g = 566.1 mg: It is known
that: Y (µg) pure Ibuprofen → a = 0.0516 g ibuprofen powder,
then in mc = 0.5661 g average weight of a pharmaceutical
tablet were: Y1 = (mc. Y) / a Ibuprofen (4). Y1 = (0.5661
36273.33) / 0.0516. Thus, Y1 = 397952.173 µg of pure
Ibuprofen / pharmaceutical tablet = 397.952 mg of pure
Ibuprofen/ pharmaceutical tablet, as a final result (TABLE
VI).
TABLE VI. CALCULTATION OF IBUPROFEN PURE CONTENT BY FILM-COATED
TABLET
Analyzed
sample solution
of Ibuprofen
Calculated parameters
Mean
measured
absorbance
(AP)
Sample
solution
concentration
(µg/mL)
(µg)
Ibuprofen
content /
film-coated
tablets
(mg)
Ibuprofen
content /
film-coated
tablets
Ibuprofen
Grindex®
tablets
0.273 5.441 397952.173 397.952
e. Percentage content (Z%) estimation of pure ibuprofen
in film-coated tablet, depending on Y1 from above expressed
in mg and reported to the pure official content of ibuprofen
on the pharmaceutical tablet, that was 400 mg:
For 100 % content → 400 mg of pure Ibuprofen, then for
Y1 (mg) Ibuprofen it was: Z = (Y1 .100) / 400 % = (Y1 / 4)
% (5). It was concluded that: Z = 397.952 / 4 = 99.488 %.
Thus, Z = 99.488 % of Pure Ibuprofen percentage content /
pharmaceutical tablet.
Statistical analysis results. Linearity of the method.
Spectrophotometric analysis of Ibuprofen has shown very
good linearity, regression coefficient value was R2
= 0.9994
(fig. 4). R2
≥ 0.9990. The standard error of the regression line
(SE) was SE = 0.004974, which had a corresponding, highly
low value (Table III).
Detection limit (LD) and quantitation limit (LQ) were
calculated with formulas (A) and (B) written above. LD =
0,383 μg/ mL and LQ = 1,275 μg/ mL, have been within the
normal limits. Stability of the standard solutions. Following
the calculation, it was found that the value of the SD standard
deviation = 0.003871, and the relative standard deviation was
calculated with the formula (C) as follows: RSD = (0.003871.
100) / 0.2671 = 1.4493%. Thus, RSD = 1.4493%. It was
found, according to table IV, that the solutions were stable
during at least 32 hours from their preparation.
System Precision estimation The absorbances
corresponding to the chosen standard solution 1.50 μg/ mL
proved to be very close to each other (Table VI). According to
formula (C) the relative standard deviation RSD = (0.003050.
100) / 0.1146 = 2.6614%. Thus, RSD = 2.6614%, was within
the normal limits, RSD ≤. 5% (Table V).
IV. CONCLUSIONS
It was found an amount of 397.952 mg of pure Ibuprofen
content / film-coated tablet. This value was very close to
Ibuprofen content declared by the pharmaceutical
manufacturer (400 milligrams), with a mean percentage
deviation of only 0.512 % from the officially declared amount
of active substance and felt within the normal limits (˂ 5%).
The applied analysis method was linear over the entire
chosen concentration range of 0.75 μg / mL -15.00 μg / mL.
R2
= 0.999352 R2
≥ 0.9990 and the correlation coefficient R =
0.999676, R > 0.9990 have been fit perfectly in the normal
range of values. LD = 0,383 μg/ mL and LQ = 1,275 μg/ mL.
Aqueous solution of 5.0 µg/mL Ibuprofen was particularly
stable and could be stored at least 32 hours, the value of the
relative standard deviation RSD = 1.4493%, RSD ≤ 5%. The
system composed by the standard solutions of Ibuprofen and
UV-Vis Spectrophotometer presented statistically a very good
precision assigned by the standard relative deviation value,
which was RSD = 2.6614%, RSD ≤ 5%. The proposed method
can be successfully applied in practice to quantitative
Ibuprofen analysis from different pharmaceutical tablet forms,
according to the actual European and international standards.
ACKNOWLEDGMENT
This study is simply an objective scientific research paper
that does not aim to confirm or deny the official results
obtained by the pharmaceutics manufacturing company, nor
to cause any damage to its image. We, the authors, declare no
conflict of interests.
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