The document discusses the typical analysis pipeline and challenges for ChIP-seq data, which involves mapping millions of sequencing reads to the genome, detecting protein binding sites (peaks), normalizing data for comparison, and visualizing thousands of enriched regions. It provides an overview of common tools for each step, including Bowtie for mapping, MACS for peak calling, and CEAS or UCSC Genome Browser for visualization. An example analysis workflow is shown that filters read qualities and parses the data.
The document contains a series of short, disjointed sentences in multiple languages including English and Chinese about various topics such as numbers, dates, and places. It does not seem to form a coherent paragraph or convey a clear message on any particular topic.
The document provides an overview of Chip-seq data analysis. It discusses the Chip-seq technology, visualization of genomic data, command line analysis including quality checking, alignment, peak calling, annotation, and motif finding. It also discusses downstream analysis such as comparing samples, analyzing region occupancy, and web resources for Chip-seq analysis.
Next-generation genomics: an integrative approachHong ChangBum
This document summarizes a presentation on next-generation genomics and integrative analysis. It discusses the types of genomic data available from techniques like genome sequencing, RNA sequencing, ChIP-seq, and epigenomics. It explains that integrative analysis can help annotate functional features, infer variant function, and understand gene regulation. Approaches to integration include data reduction, unsupervised clustering, and supervised Bayesian networks. Large-scale datasets can be accessed through browsers, add-ons, and standalone tools to generate novel hypotheses. Future work includes more integrated community resources with search capabilities.
This document discusses the bioinformatics analysis of ChIP-seq data. It begins with an overview of ChIP-seq experiments and the major steps in processing and analyzing the sequencing data, including quality control, alignment, peak calling, and downstream analyses. Pipelines for automated analysis are described, such as Cluster Flow and Nextflow. The talk emphasizes that there is no single correct approach and the analysis depends on the biological question and experimental design.
This document discusses several transcription factors involved in hematopoiesis and lymphocyte development, including HOXB4, PU.1, E2A, EBF, Pax5, Bcl-6, and Blimp-1. It describes how these transcription factors regulate gene expression and cellular differentiation at different stages of blood cell development, often through complex regulatory networks and interactions with other transcription factors. Mutation or misregulation of these transcription factors can lead to blood cell disorders or cancers.
1. Sequence analysis was performed on DNA from Glycine max (soybean) cultured cells using T3 and T7 primers, and the resulting sequences were edited and aligned.
2. The edited sequences showed high similarity, with some discrepancies, and contained restriction enzyme cut sites for EcoRI and XhoI as well as a polyA tail.
3. Multiple sequence alignments identified differences between the T3 and T7 sequences, with the greatest similarity from positions 51-900.
O Município de Urussanga, em ação civil pública movida pelo Ministério Público de Santa Catarina (MPSC), foi condenado a adequar a acessibilidade de todas as 10 unidades básicas de saúde municipais. A sentença, confirmada por acórdão do Tribunal de Justiça de Santa Catarina, fixou o prazo de 12 meses para a execução das obras de adaptação necessárias.
This document provides instructions for transforming Lactobacillus bacteria using electroporation. Key steps include inoculating a bacterial culture, harvesting cells at stationary phase, washing the cells, subjecting them to thermal shock and electroporation with plasmid DNA, incubating to allow for expression, then plating and selecting for transformed colonies using antibiotic resistance. The overall process is estimated to take 3-4 days.
The document contains a series of short, disjointed sentences in multiple languages including English and Chinese about various topics such as numbers, dates, and places. It does not seem to form a coherent paragraph or convey a clear message on any particular topic.
The document provides an overview of Chip-seq data analysis. It discusses the Chip-seq technology, visualization of genomic data, command line analysis including quality checking, alignment, peak calling, annotation, and motif finding. It also discusses downstream analysis such as comparing samples, analyzing region occupancy, and web resources for Chip-seq analysis.
Next-generation genomics: an integrative approachHong ChangBum
This document summarizes a presentation on next-generation genomics and integrative analysis. It discusses the types of genomic data available from techniques like genome sequencing, RNA sequencing, ChIP-seq, and epigenomics. It explains that integrative analysis can help annotate functional features, infer variant function, and understand gene regulation. Approaches to integration include data reduction, unsupervised clustering, and supervised Bayesian networks. Large-scale datasets can be accessed through browsers, add-ons, and standalone tools to generate novel hypotheses. Future work includes more integrated community resources with search capabilities.
This document discusses the bioinformatics analysis of ChIP-seq data. It begins with an overview of ChIP-seq experiments and the major steps in processing and analyzing the sequencing data, including quality control, alignment, peak calling, and downstream analyses. Pipelines for automated analysis are described, such as Cluster Flow and Nextflow. The talk emphasizes that there is no single correct approach and the analysis depends on the biological question and experimental design.
This document discusses several transcription factors involved in hematopoiesis and lymphocyte development, including HOXB4, PU.1, E2A, EBF, Pax5, Bcl-6, and Blimp-1. It describes how these transcription factors regulate gene expression and cellular differentiation at different stages of blood cell development, often through complex regulatory networks and interactions with other transcription factors. Mutation or misregulation of these transcription factors can lead to blood cell disorders or cancers.
1. Sequence analysis was performed on DNA from Glycine max (soybean) cultured cells using T3 and T7 primers, and the resulting sequences were edited and aligned.
2. The edited sequences showed high similarity, with some discrepancies, and contained restriction enzyme cut sites for EcoRI and XhoI as well as a polyA tail.
3. Multiple sequence alignments identified differences between the T3 and T7 sequences, with the greatest similarity from positions 51-900.
O Município de Urussanga, em ação civil pública movida pelo Ministério Público de Santa Catarina (MPSC), foi condenado a adequar a acessibilidade de todas as 10 unidades básicas de saúde municipais. A sentença, confirmada por acórdão do Tribunal de Justiça de Santa Catarina, fixou o prazo de 12 meses para a execução das obras de adaptação necessárias.
This document provides instructions for transforming Lactobacillus bacteria using electroporation. Key steps include inoculating a bacterial culture, harvesting cells at stationary phase, washing the cells, subjecting them to thermal shock and electroporation with plasmid DNA, incubating to allow for expression, then plating and selecting for transformed colonies using antibiotic resistance. The overall process is estimated to take 3-4 days.
1) The document discusses several topics related to an upcoming meeting, including the agenda, dates, and locations.
2) Key items on the agenda are a report from the director, a discussion of new initiatives, and a review of budgets.
3) The director will provide an overview of current activities and progress made on ongoing projects.
The document provides details of the Nandawadagi Lift Irrigation Scheme in 3 stages. Stage 1 includes the head works like intake canal, pump house, rising main, pumping machinery, delivery chamber, transmission line and substation. Stage 2 lists the physical progress of components as 100% completion. Stage 3 summarizes the scheme by dividing the command area into 3 blocks and the proposed allocation of irrigation potential to different areas.
The document discusses an automated system for controlling electrical energy. It describes how electrical energy is produced and consumed, and some challenges with electrical grids. It then introduces an automated system for managing electrical energy production and distribution that aims to increase efficiency and reduce costs and environmental impact. Key features of the system include automated monitoring and control of energy production facilities, centralized data collection and analysis, and automated distribution of energy based on demand.
The TRP53 gene contains 11 exons and 10 introns. Exon 1 is 128 base pairs and exon 11 is 523 base pairs, which is the longest exon. The introns range in size from 5 base pairs to 793 base pairs. The gene is located on chromosome 11 in humans and encodes a tumor protein involved in regulating the cell cycle and suppressing tumor formation.
Site directed mutagenesis of β2-microglobulin PowerPoint PresentationTyler Liang
This document describes site-directed mutagenesis experiments performed on beta-2 microglobulin (β2m) to study its role in amyloidosis. Primers were designed to introduce mutations D39V and I5T into β2m. Polymerase chain reaction and transformation were used to generate mutant plasmids, which were sequenced to confirm the mutations. Future studies are planned to introduce mutations W61F and W96F to further study β2m stability and aggregation.
1. Academia has different data needs and constraints than industry, including heterogeneous systems, slow adoption of new technologies, and data management as an afterthought.
2. The main players in academia are large genome centers, individual "genome hackers", and bench scientists, with varying levels of investment in infrastructure and optimization.
3. Genomic data comes in many formats, especially flat text files with standardized formats like VCF for storing variant calls.
O Photoshop, editor de fotografias mais usado e famoso, está passando por mudanças bastante importantes para os seus usuários com a adoção de um novo sistema de compra e utilização, a Creative Cloud. Agora, você paga por mês para usar os softwares da Adobe em planos mais acessíveis. Você pode pagar por apenas um software ou vários, sendo que o preço sofre alteração para cada tipo de conta. Todas elas possuem acesso direto à rede Behance Network, um portal de divulgação artística voltado principalmente para designers.
The document summarizes the agenda for a church meeting. It includes:
1. Opening prayer and welcome by Pastor Smith
2. Reports from the building committee, treasurer, and children's ministry director
3. Closing prayer by an elder of the church
The document discusses different data formats used for storing genomic and biological data, including flat text files, variant call format (VCF) files, and JSON documents. It provides examples of data stored in each format, such as genomic sequences, variant calls, and metadata. The formats vary in their structure, from unstructured text to structured fields. Properly formatting and organizing biological data allows for efficient analysis and sharing of information between researchers.
This document reports statistics from various regions in Mongolia in 2016. It provides data on population, health, economic activity, and transportation. Some key details include:
- The population of Ulaanbaatar was 1,224,629 in 2016, with 50.3% living in ger districts and 22.7% living in apartment blocks.
- In western Mongolia, the average age was 29.8 years for herders with livestock between 0-5 horses. Herders with 1-5 horses accounted for 25% of the population.
- Transportation statistics show 513 vehicles were registered in western Mongolia in 2016, with 61.8% being used for cargo transport and 14.7%
Smart cards are credit card sized devices that can store and process data securely. They contain embedded microchips and memory to store user identification details like PIN codes. Smart cards use cryptographic techniques like DES, RSA, and SHA standards to encrypt data and authenticate users. They have on-board security features like secure application modules (SAMs) and personal identification numbers (PINs) to prevent unauthorized access. Common uses of smart cards include payment cards, ID cards, SIM cards, and access cards.
RNASeqR: RNA-Seq workflow for case-control studyKuanHaoChao
This R package is designed for case-control RNA-Seq analysis (two-group). There are six steps: "RNASeqRParam S4 Object Creation", "Environment Setup", "Quality Assessment", "Reads Alignment & Quantification", "Gene-level Differential Analyses" and "Functional Analyses". Each step corresponds to a function in this package. After running functions in order, a basic RNASeq analysis would be done easily.
Metagenomic projects provide a unique window into the genetic composition of microbial communities. To date, metagenomic analyses have focused primarily on studying the composition of microbial populations and inferring shared metabolic pathways. In this work we analyze how high-quality metagenomic data can be leveraged to infer the composition of transcriptional regulatory networks through a combination of in silico and in vitro methods. Using the SOS response as a case example, we analyze human gut microbiome data to determine the composition of the SOS meta-regulon in a natural context. Our analysis provides proof of concept that the existing knowledgebase on regulatory networks and reference genomes can be effectively leveraged to mine meta-genomic data and reconstruct multi-species regulatory networks. This approach allows us to identify de novo the core elements of the human gut SOS meta-regulon, highlighting the relevance of error-prone polymerases in this stress response, and identifies putative novel SOS protein clusters involved in cell wall biogenesis, chromosome partitioning and restriction modification. The methodology implemented in this work can be applied to other metagenomic datasets and transcriptional systems, potentially providing the means to compare regulatory networks across metagenomes. The use of metagenomic data to analyze transcriptional regulatory networks provides a realistic snapshot of these systems in their natural context and allows probing at their extended composition in non-culturable organisms, yielding insights into their interconnection and into the overall structure of transcriptional systems in microbiomes.
Cameroun - Repertoire des projets prioritaires à besoins de financementinvestincameroon
The document is composed primarily of strings of random characters and symbols with no discernible meaning. It references various strings of letters and symbols but lacks any coherent sentences, structure or meaning.
how to analyze the data which is available with the wet lab results and we can analyze more by using bioinformatics tools. here we can learn how to analyze the unknown data.
This document provides statistics on various topics in three languages from 2016. It reports data on internet usage, mobile phone adoption, economic indicators and more. Some key findings include:
- In 2016, internet usage in the country reached 27.1 million people and mobile phone penetration was at 26.6 per 100 people.
- Mobile phone usage grew but fixed broadband internet declined. The most popular applications were social media, messengers and online video.
- Mobile phone and internet access has expanded across demographics and regions, though a digital divide still exists between urban and rural areas.
The document describes the process of transcription and translation in a cell. It shows how DNA is transcribed into mRNA by RNA polymerase in the nucleus. The mRNA is then exported through the nuclear pore into the cytoplasm. In the cytoplasm, ribosomes translate the mRNA into a protein by linking amino acids specified by the mRNA codons.
Khóa luận tốt nghiệp Quản trị kinh doanh: Hoàn thiện công tác đào tạo nguồn nhân lực tại công ty TNHH MTV xi măng Sông Gianh cho các bạn làm luận văn tham khảo
1) The document discusses several topics related to an upcoming meeting, including the agenda, dates, and locations.
2) Key items on the agenda are a report from the director, a discussion of new initiatives, and a review of budgets.
3) The director will provide an overview of current activities and progress made on ongoing projects.
The document provides details of the Nandawadagi Lift Irrigation Scheme in 3 stages. Stage 1 includes the head works like intake canal, pump house, rising main, pumping machinery, delivery chamber, transmission line and substation. Stage 2 lists the physical progress of components as 100% completion. Stage 3 summarizes the scheme by dividing the command area into 3 blocks and the proposed allocation of irrigation potential to different areas.
The document discusses an automated system for controlling electrical energy. It describes how electrical energy is produced and consumed, and some challenges with electrical grids. It then introduces an automated system for managing electrical energy production and distribution that aims to increase efficiency and reduce costs and environmental impact. Key features of the system include automated monitoring and control of energy production facilities, centralized data collection and analysis, and automated distribution of energy based on demand.
The TRP53 gene contains 11 exons and 10 introns. Exon 1 is 128 base pairs and exon 11 is 523 base pairs, which is the longest exon. The introns range in size from 5 base pairs to 793 base pairs. The gene is located on chromosome 11 in humans and encodes a tumor protein involved in regulating the cell cycle and suppressing tumor formation.
Site directed mutagenesis of β2-microglobulin PowerPoint PresentationTyler Liang
This document describes site-directed mutagenesis experiments performed on beta-2 microglobulin (β2m) to study its role in amyloidosis. Primers were designed to introduce mutations D39V and I5T into β2m. Polymerase chain reaction and transformation were used to generate mutant plasmids, which were sequenced to confirm the mutations. Future studies are planned to introduce mutations W61F and W96F to further study β2m stability and aggregation.
1. Academia has different data needs and constraints than industry, including heterogeneous systems, slow adoption of new technologies, and data management as an afterthought.
2. The main players in academia are large genome centers, individual "genome hackers", and bench scientists, with varying levels of investment in infrastructure and optimization.
3. Genomic data comes in many formats, especially flat text files with standardized formats like VCF for storing variant calls.
O Photoshop, editor de fotografias mais usado e famoso, está passando por mudanças bastante importantes para os seus usuários com a adoção de um novo sistema de compra e utilização, a Creative Cloud. Agora, você paga por mês para usar os softwares da Adobe em planos mais acessíveis. Você pode pagar por apenas um software ou vários, sendo que o preço sofre alteração para cada tipo de conta. Todas elas possuem acesso direto à rede Behance Network, um portal de divulgação artística voltado principalmente para designers.
The document summarizes the agenda for a church meeting. It includes:
1. Opening prayer and welcome by Pastor Smith
2. Reports from the building committee, treasurer, and children's ministry director
3. Closing prayer by an elder of the church
The document discusses different data formats used for storing genomic and biological data, including flat text files, variant call format (VCF) files, and JSON documents. It provides examples of data stored in each format, such as genomic sequences, variant calls, and metadata. The formats vary in their structure, from unstructured text to structured fields. Properly formatting and organizing biological data allows for efficient analysis and sharing of information between researchers.
This document reports statistics from various regions in Mongolia in 2016. It provides data on population, health, economic activity, and transportation. Some key details include:
- The population of Ulaanbaatar was 1,224,629 in 2016, with 50.3% living in ger districts and 22.7% living in apartment blocks.
- In western Mongolia, the average age was 29.8 years for herders with livestock between 0-5 horses. Herders with 1-5 horses accounted for 25% of the population.
- Transportation statistics show 513 vehicles were registered in western Mongolia in 2016, with 61.8% being used for cargo transport and 14.7%
Smart cards are credit card sized devices that can store and process data securely. They contain embedded microchips and memory to store user identification details like PIN codes. Smart cards use cryptographic techniques like DES, RSA, and SHA standards to encrypt data and authenticate users. They have on-board security features like secure application modules (SAMs) and personal identification numbers (PINs) to prevent unauthorized access. Common uses of smart cards include payment cards, ID cards, SIM cards, and access cards.
RNASeqR: RNA-Seq workflow for case-control studyKuanHaoChao
This R package is designed for case-control RNA-Seq analysis (two-group). There are six steps: "RNASeqRParam S4 Object Creation", "Environment Setup", "Quality Assessment", "Reads Alignment & Quantification", "Gene-level Differential Analyses" and "Functional Analyses". Each step corresponds to a function in this package. After running functions in order, a basic RNASeq analysis would be done easily.
Metagenomic projects provide a unique window into the genetic composition of microbial communities. To date, metagenomic analyses have focused primarily on studying the composition of microbial populations and inferring shared metabolic pathways. In this work we analyze how high-quality metagenomic data can be leveraged to infer the composition of transcriptional regulatory networks through a combination of in silico and in vitro methods. Using the SOS response as a case example, we analyze human gut microbiome data to determine the composition of the SOS meta-regulon in a natural context. Our analysis provides proof of concept that the existing knowledgebase on regulatory networks and reference genomes can be effectively leveraged to mine meta-genomic data and reconstruct multi-species regulatory networks. This approach allows us to identify de novo the core elements of the human gut SOS meta-regulon, highlighting the relevance of error-prone polymerases in this stress response, and identifies putative novel SOS protein clusters involved in cell wall biogenesis, chromosome partitioning and restriction modification. The methodology implemented in this work can be applied to other metagenomic datasets and transcriptional systems, potentially providing the means to compare regulatory networks across metagenomes. The use of metagenomic data to analyze transcriptional regulatory networks provides a realistic snapshot of these systems in their natural context and allows probing at their extended composition in non-culturable organisms, yielding insights into their interconnection and into the overall structure of transcriptional systems in microbiomes.
Cameroun - Repertoire des projets prioritaires à besoins de financementinvestincameroon
The document is composed primarily of strings of random characters and symbols with no discernible meaning. It references various strings of letters and symbols but lacks any coherent sentences, structure or meaning.
how to analyze the data which is available with the wet lab results and we can analyze more by using bioinformatics tools. here we can learn how to analyze the unknown data.
This document provides statistics on various topics in three languages from 2016. It reports data on internet usage, mobile phone adoption, economic indicators and more. Some key findings include:
- In 2016, internet usage in the country reached 27.1 million people and mobile phone penetration was at 26.6 per 100 people.
- Mobile phone usage grew but fixed broadband internet declined. The most popular applications were social media, messengers and online video.
- Mobile phone and internet access has expanded across demographics and regions, though a digital divide still exists between urban and rural areas.
The document describes the process of transcription and translation in a cell. It shows how DNA is transcribed into mRNA by RNA polymerase in the nucleus. The mRNA is then exported through the nuclear pore into the cytoplasm. In the cytoplasm, ribosomes translate the mRNA into a protein by linking amino acids specified by the mRNA codons.
Khóa luận tốt nghiệp Quản trị kinh doanh: Hoàn thiện công tác đào tạo nguồn nhân lực tại công ty TNHH MTV xi măng Sông Gianh cho các bạn làm luận văn tham khảo
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20091110 Technical Seminar ChIP-seq Data Analysis
1. Tools and challenges for
ChIP-seq data analysis
Alba Jené Sanz
Biomedical Genomics Lab (UPF)
2. Overview
1. ChIP-seq – The basics
2. Typical pipeline
3. Challenges in ChIP-seq data analysis
4. To take into account
5. Available tools
6. Analysis example
7. Future Challenges
8. Where to look for help
15. 3. Challenges in ChIP-seq data analysis
Millions of segments that need a fast mapping to the genome (allowing
mismatches or gaps, performance issues)
Peak detection – find the exact binding site
Data normalization – compare results, background noise
Visualization – thousands of enriched regions. UCSC, JBrowse…
16. 4. To take into account
Transcription Factors vs Nucleosomes / Histone modifications
Control available?
Sequencing depth bias in Control vs IP
Different alignment methods produce different peak calling results, but the difference is
not as much as the one due to different peak caller or replicate
Many differences on peak callers can be explained by the different thresholds used
Some peak callers may be specific to some data types
Consistency may be used to set threshold if replicates are available
17. 4. To take into account
There are many tools for the analysis of ChIP-
seq data, but no standards yet
22. 5. Available tools
Uses regional averaging to mitigate sample fluctuations in the control library
Uses the control to model the distribution across the genome using the Poisson
distribution (BG). After identifying candidate peaks significantly enriched over the
BG, a local labda is estimated using windows around each peak to eliminate local
biases
Open-source, open to contributions (Artistic License) and being actively
improved
Easy to use and fast-responding developers
Compares very well to other methods
29. 6. Analysis example
MACS pipeline
Output:
- Peak locations in BED and XLS format (genome browser)
- Tag count in wiggle format (genome browser)
- Bimodal model in R scripts
40. 7. Future challenges
Re-analyze data with new algorithms – sequences remain the same
ChIP-seq combined with Chromatin Conformation Capture (3C) –
long-range physical interactions
Technical improvements: RNA-seq will benefit from longer reads
Integrated computational analyses – integration of TF, histone
marks, methylation, polymerase loading to predict regulatory output