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1. Why is there a color difference in Serratia marcescens grown at the two different
temperatures? 2. Was E . coli killed when incubated at 4 C ? If yes, how? If no, what specifically
prevented it from growing? 3. Which organism was more heat resistant in the Lethal Effects of
Temperature experiment? Why? Briefly define the following terms: 4. mesophile: 5. thermal
death time: 6. thermal death point: 7. Organisms that grow at 0 C and have optimum growth
temperature of 1 5 C or lower are called 8. Based on your class data- describe the follow-up
experiment that would allow you to determine TDP (thermal death point) of E.coli 9. What are
psychrotrophic organisms? What is their importance? 0. What is the optimum temperature of
growth of most pathogens? Why?

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