The document describes the three types of hemolysis observed on blood agar: alpha, beta, and gamma. It also defines selective and differential media, provides examples of media used to isolate specific bacteria (E. coli, Neisseria gonorrhoeae), and discusses whether EMB agar can be used to isolate MRSA. It also describes the expected color changes on Triple Sugar Iron agar for Salmonella and defines a fastidious organism.

1. Answer: Describe The Three Different Types Of Hemolysis Observed On
Answer:
Describe The Three Different Types Of Hemolysis Observed On Blood Agar.
The utilization of blood agar media to observe hemolytic responses is a highly effective
method for identifying bacteria, especially streptococci (Marques-Garcia 2020). The
following are the several forms of hemolysis:
Alpha (α) Hemolysis:All around colonies, an unclear zone of incomplete disintegration of
red blood cells appears, frequently associated by a green to brown staining of the media.
S. pneumonia, as well as many other streptococci related to oral transmission, include
hemolytic bacteria.
Beta (β) Hemolysis:All around the colonies formed, a clear, colourless zone develops,
indicating that the Red Bloods Cells have been entirely lysed. Streptococcus pyogenes,
Streptococcus agalactiae, and numerous different streptococci subspecies are hemolytic
(Marques-Garcia 2020). Several bacteria apart from streptococcus species can be hemolytic,
notably Pseudomonas aeruginosa, Staphylococcus aureus, and Listeria monocytogenes, and
hemolytic responses are valuable screening tools for bacteria.
No (γ) Hemolysis:The colonies produce no visible hemolytic function or discolouration. This
type of hemolysis is also known as gamma hemolysis.
What Is A Selective Medium?
Selective media has substances that avoids some microorganisms from growing while
supporting others to thrive at the same time. The elevated salt level for mannitol salt agar,
such as when it inhibits the development of many microorganisms yet allows staphylococci
to proliferate (Knobloch-Westerwick, Westerwick and Sude et al. 2020).
What Is A Differential Medium?
Differential media has differential substances that supports groupings of microorganisms to
become visibly discriminated through the look of the colonies or accompanying media, due
to a differential among the two sets. One sort of differential media is blood agar that helps
bacteria to be identified according to the form of hemolysis they cause. Many common
enteric agars, like MacConkey & EMB agars, primarily include selective with gram (-)

2. coliforms and distinguish lactose-fermenting and non-lactose fermenting bacterium
(Knobloch-Westerwick, Westerwick and Sude et al. 2020).
Which Media Can Be Used To Isolate E. Coli Samples From Contaminated Lettuce?
MacConkey Agar can be used to isolate samples of E.coli from a contaminated sample of
Lettuce. MacConkey Agar is a selective and differential media containing bile salts and
crystal violet pigment that is beneficial in contrasting gram-negative bacteria such as E.coli
by differentiating lactose fermenting and non-fermenting species. Chooses the best for non-
meticulous gram-negatives; red colonies suggest lactose fermented, whereas white colonies
suggest no fermentation (Jung and Hoilat 2021).
Which Two Media Would Be Used To Identify A Sample Taken From A Patient With
Suspected Gonorrhoea?
Thayer-Martin Agar and Chocolate agar will be used to identify gonorrhoea suspected
patients (Mowlaboccus 2018). Both these media are enriched.
Thayer-Martin Agar:
They are the antibiotic-rich and selective medium that includes colistin (which destroys
total gram-negative coliform), vancomycin (which destroys gram-positive bacteria), and
nystatin (which destroys fungus). Inpatient specimens comprising vast quantities of
commensal bacteria, like the female vaginal tract, this type of medium is preferable to
choose for intolerant organisms like N. gonorrhoea.
Chocolate Agar:
The main purpose of using chocolate agar is to Culture picky organisms like Neisseria
gonorrhoeae since chocolate agar supports the growth of organisms that would not thrive
on a normal medium (Mowlaboccus 2018).
Would You Be Able To Grow A Sample Obtained From A Patient's Wound (Suspected To Be
Infected With MRSA) On EMB? Explain.
No, a sample collected from a patient's wound presumed to be affected with MRSA cannot
be grown on EMB. Blood agar, mannitol salt agar, and Baird-Parker agar bearing methicillin
alone or other antibiotics have typically been used to isolate MRSA (Deniz et al. 2021). The
Eosin Methylene Blue Agar is used for selective staining for gram-negative bacteria, so it
cannot be used for gram-positive MRSA. Therefore, either of the mentioned agar can be
used (Knobloch-Westerwick, et al., 2020).
What Is The Color Or TSI For Salmonella?

3. The Triple Sugar Iron test is a microbial assay that evaluates a microorganism's capacity to
ferment glucose and produces hydrogen sulfide. It is frequently utilized to differentiate
enteric bacteria like Salmonella. Since a prolonged incubation period might result in a false
negative, TSI Agar media should solely be analyzed after twenty-fours incubation times
(Alexan 2017).
The following table shows the color or TSI for salmonella:
Observation
Inference
Yellow slant/yellow butt
Fermentation of lactose, dextrose, and sucrose (optional).
Red slant/yellow butt
Fermentation of dextrose
Red slant, red butt
Absence of the fermentation of carbohydrates.

4. Blackening of the medium
Presence of hydrogen gas.
What Is A Fastidious Organism?
Any microorganism with complicated or specific dietary needs is called a fastidious
organism. Put another way; a picky creature will only thrive if specified resources are
present in its environment. Microbes that will solely develop if specific nutrients are
available in the culture medium are fastidious organisms. As a result, fastidiousness is
frequently regarded as impossible to cultivate using any available approach (Lainhart and
Burnham 2018).
References:
Alexan, A.F., 2017. Lactose fermenting Salmonella typhimurium, Salmonella stratford and
Salmonella blegdam. Egyptian Journal of Agricultural Research, 95(3), pp.1285-1296.
Deniz, N., Bayram, Y., Parlak, M., Irden, ?. and Güdücüo?lu, H., 2021. The determination of
meca gene presence in mrsa strains isolated from intensive care unit by conventional,
automated and pcr method. Eastern Journal of Medicine, 26(1).
Jung, B. and Hoilat, G.J., 2021. MacConkey Medium. StatPearls [Internet].
Knobloch-Westerwick, S., Westerwick, A. and Sude, D.J., 2020. Media choice and selective
exposure. Media effects: Advances in theory and research, pp.146-162.
Lainhart, W. and Burnham, C.A.D., 2018. Enhanced recovery of fastidious organisms from
urine culture in the setting of total laboratory automation. Journal of clinical
microbiology, 56(8), pp.e00546-18.
Marques-Garcia, F., 2020. Methods for hemolysis interference study in laboratory medicine–
a critical review. Ejifcc, 31(1), p.85.
Mowlaboccus, M., 2018. Molecular Epidemiology of, and Antimicrobial Resistance in,
Pathogenic Neisseria meningitidis from Western Australia.