Despite greatly improved understanding of endothelial heterogeneity, the number of molecules discriminating human arterial and venous endothelium remains limited. Indeed, there have been few reports validating markers proposed in animal model studies in freshly isolated human tissues. We report here the global characterization of freshly isolated human umbilical arterial and venous endothelial cell (HUAECs and HUVECs) plasma membrane proteins using an experimentally validated label-free quantitative LC-MS/MS platform.

1. Open Journal of
Proteomics
Peertechz Journals

2. RESEARCH ARTICLE
Proteomic Analysis Reveals GLUT1 to be a Novel
Discriminating Marker of Human Arterial Endothelium In
vivo and loss of Venous Identity in Cell Culture
 Richardson MR, Cahan P, Lai X, Yoder MC, Witzmann FA
 *Corresponding author: Frank A Witzmann, PhD, Department of
Cellular and Integrative Physiology, Indiana University School of
Medicine 635 Barnhill Drive, Room 362A, Indianapolis, Indiana,
46202- 5120, Tel: (317) 278-5741; Fax: (317) 274-3318;
 Dates: Received: 07 July, 2016; Accepted: 01 August, 2016;
Published: 02 August, 2016
 Citation: Richardson MR, Cahan P, Lai X, Yoder MC, Witzmann FA
(2016) Proteomic Analysis Reveals GLUT1 to be a Novel
Discriminating Marker of Human Arterial Endothelium In vivo and loss
of Venous Identity in Cell Culture. Open J Proteomics 1(1): 001-007.

3. Abstract
 Despite greatly improved understanding of
endothelial heterogeneity, the number of molecules
discriminating human arterial and venous
endothelium remains limited. Indeed, there have
been few reports validating markers proposed in
animal model studies in freshly isolated human
tissues. We report here the global characterization
of freshly isolated human umbilical arterial and
venous endothelial cell (HUAECs and HUVECs)
plasma membrane proteins using an experimentally
validated label-free quantitative LC-MS/MS
platform.

4. Thank you
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