1. Ryan Daniel Whetstone MS, PhD
365 S. Pacific Ave, Apt 4
Pittsburgh, PA 15224
412-779-9852
rdw8325@gmail.com
Education:
Graduate: Doctorate of Philosophy, Pharmaceutical Sciences
Department of Pharmaceutical Sciences, School of Pharmacy
University of Pittsburgh, Pittsburgh PA, December 2014
Thesis: The Effect of Inflammation on Stem Cell Mutagenesis and
Carcinogenesis Induced by Azoxymethane in Wild Type and Immune
Compromised Mice
Masters of Science, Biotechnology
School of Arts and Sciences
Johns Hopkins University, Baltimore MD, August 2003
GPA 3.9
Undergraduate: Bachelor of Arts, Chemistry
School of Arts and Sciences
West Virginia University, Morgantown WV, August 1998
Research and Work Experience:
Fall 2007- Teaching Assistant and Graduate Student
Present Department of Pharmaceutical Sciences, School of Pharmacy
University of Pittsburgh
505 Salk Hall
3501 Terrace Street
Pittsburgh, PA 15261
Thesis: The Effect of Inflammation on Stem Cell Mutagenesis and Carcinogenesis
Induced by Azoxymethane in Wild Type and Immune Compromised Mice
2006-2007 Medical Technician
University of Pittsburgh Medical Center
St. Margaret Hospital
815 Freeport Road
Pittsburgh, PA 15215
Patient Sample Analysis identifying Bacterial Infectious Agents

2. 2004-2006 Sr. Research Technician
Battelle
505 King Avenue
Columbus, OH 43201
Research: Applied Sciences Research and Development. Biacore surface plasmon
resonance instrument training and experience
2000-2004 Sr. Research Technician
Johns Hopkins University
1101 East 33rd Street
Baltimore, MD 21218
Research: Hematopoietic oncology, Programmed Cell Death 1 Ligand/Receptor
Chemotherapeutic Topoisomerase Inhibitors
1998-2000 Sr. Research Technician
The University of Texas at San Antonio
1 UTSA Circle
San Antonio, TX 78249
Research: Growth Zone and Resting Zone Chondrocytes clinical drug induction
and antagonism
Teaching Experience:
Teaching Assistant Biochemistry 1, Biochemistry 2, Drug Development 1b, Immunology,
2008-2010 Drug Development 2b, Anatomy & Physiology 1, Oncology/Hematology
Anatomy & Physiology 2
Publications:
2015 Ryan D. Whetstone and Barry Gold (2015). T-Cells Enhance Stem Cell
Mutagenesis in the Mouse Colon. Mutation Research. Accepted for Publication
Feb 24, 2015. Ms. Ref. No.: MUT-D-14-00105R3
2015 Submitted for Publication pending review (Feb 2015)
Ryan D. Whetstone and Barry Gold (2015). Quantification of Glucose-6-
Phosphate Dehydrogenase Mutations in Colonic Stem Cells. J. Vis. Exp.
2015 Submitted for Publication pending review (March 2015)
Ryan D. Whetstone and Barry Gold (2015). Colon Carcinogenesis in Wild Type
and Immune Compromised Mice after Treatment with Azoxymethane and/or
Dextran Sulfate Sodium. J. of Carcinogenesis.

3. 2001 Schwartz, Z., Sylvia, V. L., Luna, M. H., DeVeau, P., Whetstone, R., Dean, D.
D., Boyan, B. D. (2001). "The effect of 24R,25-(OH)(2)D(3) on protein kinase C
activity in chondrocytes is mediated by phospholipase D whereas the effect of
1alpha,25-(OH)(2)D(3) is mediated by phospholipase C." Steroids 66(9): 683-
694.
2001 Sylvia, V. L., Schwartz, Z., Del Toro, F., DeVeau, P., Whetstone, R., Hardin, R.
R., Dean, D. D., Boyan, B. D. (2001). "Regulation of phospholipase D (PLD) in
growth plate chondrocytes by 24R,25-(OH)2D3 is dependent on cell maturation
state (resting zone cells) and is specific to the PLD2 isoform." Biochim Biophys
Acta 1499(3): 209-221.
Presentations:
Oral: Stem Cell Mutagenesis and Carcinogenesis in the Colon of Wild Type and
Immune Compromised Mice after Treatment with Azoxymethane and/or Dextran
Sodium Sulfate. 16th
Annual DNA Repair Meeting. Wayne State University,
Detroit MI, May 17th
and 18th
2014
The Effect of Inflammation on Stem Cell Mutagenesis and Carcinogenesis
Induced by Azoxymethane in Wild Type and Immune Compromised Mice. School
of Pharmacy, Dissertation Defense. University of Pittsburgh, Pittsburgh PA, Nov
20th
2014
α/β T-cell Mediated Survival and Enhanced Somatic Stem Cell Mutation
Frequency in Wild Type mice vs. α/β T-cell Deficient mice. School of Pharmacy,
Graduate Student Seminar. University of Pittsburgh, Pittsburgh PA, Sept 2013
Somatic Stem Cell Mutation Frequency as determined by measuring Glucose-6
Phosphate Dehydrogenase null phenotypic Mutations in Wild Type mice and α/β
T-cell Deficient mice. School of Pharmacy, Graduate Student Seminar. University
of Pittsburgh, Pittsburgh PA, Oct 2012
Colon Cancer development induced by treatment with Azoxymethane and Dextran
Sulfate Sodium is enhanced in Wild Type mice vs. Immune Compromised mice.
School of Pharmacy, Graduate Student Seminar. University of Pittsburgh,
Pittsburgh PA, Sept 2011
The effect of treating Wild Type and athymic C57Bl/6 male mice with
Azoxymethane, Dextran Sulfate Sodium, and Azoxymethane + Dextran Sulfate
Sodium to induce colon cancer development. School of Pharmacy, Graduate
Student Seminar. University of Pittsburgh, Pittsburgh PA, Oct 2010
The potential role of T-cells potentiating Colon Cancer development induced by
Azoxymethane and Dextran Sulfate Sodium. School of Pharmacy, Graduate
Student Seminar. University of Pittsburgh, Pittsburgh PA, Nov 2009

4. The role of Inflammation in increasing the risk of Colorectal Cancer
Development. School of Pharmacy, Graduate Student Seminar. University of
Pittsburgh, Pittsburgh PA, Dec 2008
Poster: Ryan Whetstone and Barry Gold. Colon Stem Cell Mutagenesis and
Carcinogenesis in Wild Type and Immune Compromised Mice after Treatment
with Azoxymethane and/or Dextran Sulfate Sodium. 16th
Annual DNA Repair
Meeting. Wayne State University, Detroit MI, May 17th
and 18th
2014
Ryan Whetstone and Barry Gold. Colon Stem Cell Mutagenesis and
Carcinogenesis in Wild Type and Immune Compromised Mice after Treatment
with Azoxymethane and/or Dextran Sulfate Sodium. 26th
Annual UPCI Scientific
Retreat. Pittsburgh PA, June 19th
and 20th
2014
Ryan Whetstone and Barry Gold. Colon Stem Cell Mutagenesis and
Carcinogenesis in Wild Type and Immune Compromised Mice after Treatment
with Azoxymethane and/or Dextran Sulfate Sodium. Science 2014. University of
Pittsburgh, Pittsburgh PA, Oct 2nd
and 3rd
2014
Professional Affiliations :
2008-Present American Association of Pharmaceutical Scientists (AAPS)
2008-Present American Association for Cancer Research (AACR)