2. Membrane Filter
• membrane filter is a poly-nuclear membrane composed
of thousands of micropores. Through membrane filter,
particles larger than the aperture are filtered onto the
surface. It is used to filter various solutions, remove
bacteria and impurities. Also, the membrane filter is a
critical part of the syringe filter and has different
parameters and types
Types of Membrane Filter
• flat membrane
• curly membrane.
3. • membrane filter is mainly domestic film, including MCE,
Nylon, PTFE, MCE,PES, CA, PVDF, GF,PP. PTFE-L,
MCE, PES, CA, PVDF
• Classification of filter membranes
• Filter membranes are classified according to the size of
retained raw water particles, and the pores of the
membranes are divided into microfiltration membrane
(MF),
• ultrafiltration membrane (UF),
• nanofiltration membrane (NF) and
• reverse osmosis membrane (RO).
4. • MF membrane has a pore diameter of 0.05um or more
or molecular weight of 1,000 or more. It is intended to
remove colloids and polymer organic matter.
• Construction
• Membrane filters are made of thin and flat membranes
of cellulose derivatives, such as, cellulose acetate and
cellulose nitrate. These filters are brittle when in dry
condition and can be stored for an indefinite period.
The filters are between 50 and 150 µ thick and are
available in sizes upto 60 cm2
5. Working:
• A membrane filter has 400 to 500 million pores per
square centimetre of filter surface. The pores are
absolutely uniform in size and occupy about 80% of
filter volume. To avoid rapid clogging of a membrane,
pre-filtration is often required. The selection of a
membrane filter for a particular application depends on
the particles to be removed.
• Uses: These filters are mainly used for sterilization of
both aqueous and oily liquids. The membrane filters
cannot be used for filtration of organic solvents, such as
alcohols, ketones, esters and chloroform
6. Components of Validation Study
• Destructive testing –
• Non destructive testing –
- Bubble point test
- Diffusion test
- Pressure Holding testing
• Water intrusion test -
• Filter Inertness –
• Operating Conditions –
• Fibers –
• Endotoxins –
• Toxicity
7. • Destructive Testing
• Select 0.22 µm filter discs
Solution of culture media -Brevundimonas diminuta -
Pseudomonas diminuta
The effluent is then passed through a second 0.45 µm
assay filter disc
placed on an agar plate and incubated