The document describes a project to develop a herbal formulation using rosemary leaves and coconut oil extracted via maceration to treat alopecia. It outlines the methodology for extracting rosemary oil from leaves, formulating the herbal hair oil, and evaluating parameters like pH, viscosity, and acid value. The results showed the herbal hair oil had pleasant odor and dark green color, indicating rosemary leaves and coconut oil could successfully be formulated to treat hair loss and promote hair growth.

1. “DEVELOPMENT OF HERBAL
FORMULATION FOR THE TREATMENT
OF ALOPECIA”
Project Work Report Submitted In The Partial Fulfillment Of Academic Curriculum Of
Final Year B. Pharmacy, Eighth Semester Of
Dr. Babasaheb Ambedkar Marathwada University, Aurangabad
Submitted by
Momin Zafirul Islam Aqueel & Omkar Dalvi
Final Y B Pharm, Semester-VIII
Guided by
Mr. Bodkhe V.D
Assistant Professor
(Dept. of Pharmaceutical Chemistry)
Anand Charitable Sanstha’s College of Pharmaceutical Sciences and
Research, Ashti 414203
2021 – 2022
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2.  Aim: Formulation and evaluation of Essential hair oil for alopecia
By Maceration Process.
 Objective:
 This Prepration Is Generally Used To Increase Growth Of Hair To Identification Of
Physical Examination And Evaluation Test Of Herbal Hair Oil.
 To Investigate The Evaluation Parameters
 From The Research; It Is Clear That Pharmacognostic Work Is Carried Out.
 The Present Study Was Therefore Undertaken To Investigate The Formulation And
Evaluation Of The Herbal Hair Oil Was Carried Out
 Rosemarry oil are hair care formulation apply for cure of hair disorder such as
alopecia , hair fall, baldness
 Extraction of Oil From Rosemary Leaves by the process of Maceration.
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3. Literature review: formulation Of Herbal Prepration of Oil
Collection and preparation of Rosmary oil.
Check availability of required chemicals and equipments.
Collection and identification of drug profile and equipments.
Extraction of Oil From Rosemary Leaves by the process of
Maceration..
Evaluation parameters of Rosemary Oil.
Determination of pH.
Determination of Saponification value.
Determination . Viscosity & Acid value
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4. 1) Rosemary leaves:-
a)Synonym: - Rosemarinus officinalis
B) Family: - Mint Family Lamiaceae
c) Biological source :-
d) Cosmetic Uses : Stimulant, Analgesic, Anti-inflammatory,
Antiseptic, Anti-fungal, Anti-bacterial, Astringent, Disinfectant,
Antioxidant.
Odorous: Anti-stress, Cognition-enhancement, Psycho-stimulant,
Stimulant, Decongestant.
2.Methyl acetate:
It is a carboxylate ester with the formula CH₃COOCH₃.
It is a flammable liquid with a characteristically pleasant
smell.
•Used in the manufacturing of artificial leather.
•Used for the biodegradation of organic materials.
•Used as a plasticizer.
•Used to manufacture Lubricants. 4

5.  Requirements
 Ingredient:-Rosemary Leaves
 Chemicals: Mehtyl Acetate, Rose Water ,
 Equipments : Weighing balance, Beaker,Mortel Pistle, pH
meter, Measuring cylinder, Petri plate, Glass rod, Mixer, Nesseler
cylinder, ,Seprating Funnel,Glass Bottle
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6. FIG 1.COMMEN METHOD FOR EXTACTION OF OIL
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7. Step 1
Plant material is finely cut, crushed, or
ground into moderately coarse powder.
Step 2
•Solvent (Methyl Acetate) is added in
sufficient amount.
•Step 3
•The mixture is allowed to stand for 48
hours and is shaken occasionally.
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8. Step 4
•The liquid is strained.
•Step 5
•Formulation residue (Marc) is pressed
at Mortal pastel to Extarcting
remaining Oil.
Step 6
Put in open for
evaporation of Solvent
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9. .
Step 8
Liquids are clarified through
filtration or subsidence.
Step 7
Filtration Strained and expressed iquids
are mixed.
Step 9
•Adding ingredients.
1.Coconut oil
2.Fregrence (Rosewater)
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10. Step 10
pH Determination by blue litmus paper.
•Final formulation •Rosemary Residue after the
extracting Oil Content.
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11. Sr no Name Quantity
1 Rosemarry dried Leaves 40 g
2 Methyl Acetate 100 ml
3 Coconut Oil 5ml
4 Fregrence Rosewater 5ml
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12. Sensitivity Test
The Prepared Herbal Hair Oil Was Applied On 1 Cm Skin Of Hand And Exposed To
Sunlight For 4-5 Min.
Acid Value
Preparation Of 0.1 Molar Solution: Weighed 0.56 G KOH Pellets And Dissolved In 100
Ml Of Distilled Water And Stirred Continuously. The Prepared 0.1 Molar KOH Solution
Was Filled In The Burette. Preparation Of Sample: Measured 10 Ml Oil And Dissolved In
25 Ml Of Ethanol And 25 Ml Of Ether Mixture And Shacked. Added 1 Ml Of
Phenolphthalein Solution And Titrated With 0.1 Molar KOH Solution.
Saponification Value
Accurately Weighed 1 Ml Of Oil Into A 250 Ml Of Conical Flask And 10 Ml Of Ethanol :
Ether Mixture (2 : 1) Was Added. To This Flask 25 Ml Of 0.5 N Alcoholic KOH Was.
Kept The Flask For 30 Min. And The Flask Was Cooled. The Cooled Solution Was
Titrated Against 0.5 N Hcl Using Phenolphthalein Indicator.
Ph: The Ph Of Herbal Hair Oil Was Determined Using Ph Meter.
Viscosity: The Viscosity Was Determined Using Ostwald’s Viscometer.
Specific Gravity: Take The Specific Gravity Bottle, Rinsed It With Distilled Water, Dry
It,In Oven For 15 Minutes, Cool, Closed It With Cap And Weigh It (A). Now Fill The
Same Specific Gravity Bottle Th The Sample And Closed It With Cap And Again Weigh It
(B). Determine The Weight Of Sample Per Milliliter By Subtracting The Weight 12

13. Observation table:
Rosemary oil. Rosemary oil (Rosmarinus officinalis L.) is a medicinal
plant with diverse actions, including enhancing microcapillary perfusion,
which explain its usefulness in reducing hair loss and increasing hair
growth. All above ground plant parts of rosemary contain highly
aromatic and pleasantly scented essential oil, which has numerous
applications in the commercial production of liquors, scented consumer
products, perfumes, cosmetics, and aromatherapy
Sr. No Parameter Result
1 Formulation Oil
2 Apperence Good
3 pH 7.2
4 Odour Pleasent
5 Colour Dark Green
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14.  The Idea That Rosemary Oil Encourages Hair Growth May Come
From The Rosemary’s Basic Health Benefits. The Plant In
Essential Oil Form Is Said To:
 Have Anti-inflammatory Properties
 Promote Nerve Growth
 Improve Circulation
 We Concluded That Rosemary Oil Had Anti-inflammatory
Properties And Rosemary Essential Oil Strengthens Circulation.
As A Result, It Could Prevent Hair Follicles From Being Starved
Of Blood Supply, Dying Off, And Leading To Hair Loss.
 Study Concluded That Herbal Formulation For Alopecia Can Be
Successfully Formulated Using Rosemary Leaves & Coconut Oil
By Maceration Process, It Is Also Concluded The Use Of Natural
Formulation Having Very Less Or No Side Effect.
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15.  1.. N. Sanju, N. Arun and K. K. Roop, Cosmetic Technology, 1st Edition, Birla
Publications Pvt. Ltd, Delhi (2006) pp. 379-382.
 2. S. Kaul and S. Dwivedi, Indigeneous Ayurvedic Knowledge of Some Species in
the Treatment of Human Disease and Disorders, Int. J. Pharm. Life Sci., 1(1), 44-
49 (2010).
 3. B. M. Mithal and R. N. Shah, A Hand Book of Cosmetics, 1st Edition, Vallabh
Prakashan, Delhi (2000) pp. 141-142. 4. R. Shoba Rani Hiremath Textbook of
Industrial Pharmacy, 1st Edition, Orient Longaman Pvt. Ltd., Hyderabad (2007)
pp. 99-102.
 5. S. C. Bhatia, Perfumes, Soaps, Detergents and Cosmetics, 2nd Edition, CBS
Publishers and Distributions, Delhi (2001) pp. 639- 641.
 6. Indian Pharmacopoeia, Government of India, Ministry of Health and Family
Welfare, Published by, The Controller of Publication, Edition, Vol. II (1996).
 7. R. Oprean; M. Tamas; R. Sandulescu; L. Roman “Essential oil analysis. I.
Evaluation of essential oil composition using both GC and MS “ fingerprints. J.
Pharm. Biomed.
 8. A.Tezel; A. Hortacsu; O. Hortacsu, “Multi-component models for seed and
essential oil extraction” Supercritical Fluids. 15